Name: Laceyella thermophila CCTC AB 2015040
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140689

Type strain:

Species: Laceyella thermophila

Culture col. no.: CCTC AB 2015040, NBRC 110772, YIM 79486, DSM 46882, JCM 30657, BCRC 16987, CCTCC AB 2015040

Strain history: W.-J. Li; Yunnan Inst. of Microbiol., Yunnan Univ., China; YIM 79486.

NCBI tax ID(s): 1960874 (species)

SI-ID 403481

DSM 46882

Section

Laceyella thermophila CCTC AB 2015040 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from sediment sample collected from Jinze hot spring.

Gram-positive
spore-forming
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Thermoactinomycetaceae*
	Genus	*Laceyella*
	Species	**Laceyella thermophila**
	Full Scientific Name (LPSN)	Laceyella thermophila Ming et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43458]	Gram stain	positive

[Ref.: #43458]	Incubation period	7 days
[Ref.: #43458]	Cultivation medium used	T5 agar

Multimedia content

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Caption:

Medium 65 50°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

T5 agar

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Culture medium growth

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Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

		Temperature
[Ref.: #43458]	growth	37-65 °C
[Ref.: #43458]	optimum	50 °C
[Ref.: #66517]	growth	50 °C
[Ref.: #67770]	growth	45 °C

[Ref.: #43458]	Temperature range	thermophilic
[Ref.: #66517]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #43458]			optimum	7-7.5
[Ref.: #43458]			growth	6-8

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

[Ref.: #43458]	Spore description	terminally located, produced on short sporophores
[Ref.: #43458]	Type of spore	endospore
[Ref.: #43458]	Ability of spore formation	yes

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Halophily / tolerance level

non-halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43458]		NaCl		growth	0-0.5	%(w/v)
[Ref.: #43458]		NaCl		optimum	0	%(w/v)

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Observation

quinones: MK-9, MK-8

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43458]	2-hydroxybutyrate ChEBI	-	carbon source
[Ref.: #43458]	2-oxobutanoate ChEBI	-	carbon source
[Ref.: #43458]	2-oxoglutarate ChEBI	+	carbon source
[Ref.: #43458]	3-hydroxybutyrate ChEBI	-	carbon source
[Ref.: #43458]	3-O-methyl-D-glucose ChEBI	-	carbon source
[Ref.: #43458]	4-hydroxyphenylacetic acid ChEBI	-	carbon source
[Ref.: #43458]	acetate ChEBI	-	carbon source
[Ref.: #43458]	acetoacetate ChEBI	+	carbon source
[Ref.: #43458]	bromosuccinate ChEBI	-	carbon source
[Ref.: #43458]	casein	-	degradation
[Ref.: #43458]	cellobiose ChEBI	-	carbon source
[Ref.: #43458]	cellulose ChEBI	-	degradation
[Ref.: #43458]	citrate ChEBI	-	carbon source
[Ref.: #43458]	D-arabitol ChEBI	-	carbon source
[Ref.: #43458]	D-fructose ChEBI	+	carbon source
[Ref.: #43458]	D-fructose 6-phosphate ChEBI	+	carbon source
[Ref.: #43458]	D-fucose ChEBI	+	carbon source
[Ref.: #43458]	D-galactose ChEBI	+	carbon source
[Ref.: #43458]	D-galacturonic acid ChEBI	+	carbon source
[Ref.: #43458]	D-gluconate ChEBI	-	carbon source
[Ref.: #43458]	D-glucose ChEBI	+	carbon source
[Ref.: #43458]	D-glucose 6-phosphate ChEBI	-	carbon source
[Ref.: #43458]	D-glucuronate ChEBI	+	carbon source
[Ref.: #43458]	D-mannitol ChEBI	-	carbon source
[Ref.: #43458]	D-mannose ChEBI	+	carbon source
[Ref.: #43458]	D-saccharate ChEBI	-	carbon source
[Ref.: #43458]	D-serine ChEBI	-	nitrogen source
[Ref.: #43458]	D-sorbitol ChEBI	+	carbon source
[Ref.: #43458]	dextrin ChEBI	-	carbon source
[Ref.: #43458]	esculin ChEBI	+	hydrolysis
[Ref.: #43458]	formate ChEBI	-	carbon source
[Ref.: #43458]	galactarate ChEBI	-	carbon source
[Ref.: #43458]	gamma-aminobutyric acid ChEBI	-	carbon source
[Ref.: #43458]	gelatin ChEBI	+	hydrolysis
[Ref.: #43458]	gentiobiose ChEBI	+	carbon source
[Ref.: #43458]	glucuronamide ChEBI	+	nitrogen source
[Ref.: #43458]	glycerol ChEBI	-	carbon source
[Ref.: #43458]	glycine ChEBI	-	nitrogen source
[Ref.: #43458]	glycine-proline ChEBI	+	nitrogen source
[Ref.: #43458]	inosine ChEBI	-	carbon source
[Ref.: #43458]	L-alanine ChEBI	+	nitrogen source
[Ref.: #43458]	L-arginine ChEBI	+	nitrogen source
[Ref.: #43458]	L-asparagine ChEBI	+	nitrogen source
[Ref.: #43458]	L-cystine ChEBI	-	nitrogen source
[Ref.: #43458]	L-fucose ChEBI	+	carbon source
[Ref.: #43458]	L-galactonic acid gamma-lactone ChEBI	+	carbon source
[Ref.: #43458]	L-glutamate ChEBI	-	nitrogen source
[Ref.: #43458]	L-histidine ChEBI	-	nitrogen source
[Ref.: #43458]	L-isoleucine ChEBI	-	nitrogen source
[Ref.: #43458]	L-lysine ChEBI	-	nitrogen source
[Ref.: #43458]	L-methionine ChEBI	-	nitrogen source
[Ref.: #43458]	L-phenylalanine ChEBI	-	nitrogen source
[Ref.: #43458]	L-proline ChEBI	+	nitrogen source
[Ref.: #43458]	L-pyroglutamic acid ChEBI	-	nitrogen source
[Ref.: #43458]	L-rhamnose ChEBI	+	carbon source
[Ref.: #43458]	L-serine ChEBI	-	nitrogen source
[Ref.: #43458]	L-threonine ChEBI	-	nitrogen source
[Ref.: #43458]	L-tyrosine ChEBI	-	nitrogen source
[Ref.: #43458]	L-valine 2-naphthylamide ChEBI	+	nitrogen source
[Ref.: #43458]	lactate ChEBI	-	carbon source
[Ref.: #43458]	lactose ChEBI	-	carbon source
[Ref.: #43458]	malate ChEBI	-	carbon source
[Ref.: #43458]	maltose ChEBI	+	carbon source
[Ref.: #43458]	melibiose ChEBI	+	carbon source
[Ref.: #43458]	methyl (R)-lactate ChEBI	-	carbon source
[Ref.: #43458]	methyl D-glucoside ChEBI	-	carbon source
[Ref.: #43458]	myo-inositol ChEBI	-	carbon source
[Ref.: #43458]	N-acetyl-beta-D-mannosamine ChEBI	-	nitrogen source
[Ref.: #43458]	N-acetylgalactosamine ChEBI	-	nitrogen source
[Ref.: #43458]	N-acetylglucosamine ChEBI	-	nitrogen source
[Ref.: #43458]	n-acetylneuraminate ChEBI	-	nitrogen source
[Ref.: #43458]	nitrate ChEBI	-	reduction
[Ref.: #43458]	pectin ChEBI	+	hydrolysis
[Ref.: #43458]	propionate ChEBI	-	carbon source
[Ref.: #43458]	pyruvate ChEBI	+	carbon source
[Ref.: #43458]	quinate ChEBI	-	carbon source
[Ref.: #43458]	raffinose ChEBI	-	carbon source
[Ref.: #43458]	salicin ChEBI	-	carbon source
[Ref.: #43458]	stachyose ChEBI	-	carbon source
[Ref.: #43458]	sucrose ChEBI	-	carbon source
[Ref.: #43458]	trehalose ChEBI	-	carbon source
[Ref.: #43458]	turanose ChEBI	+	carbon source
[Ref.: #43458]	tween 20 ChEBI	-	degradation
[Ref.: #43458]	tween 40 ChEBI	-	degradation
[Ref.: #43458]	tween 60 ChEBI	-	degradation
[Ref.: #43458]	tween 80 ChEBI	-	degradation
[Ref.: #43458]	urea ChEBI	+	hydrolysis
[Ref.: #43458]	xylan ChEBI	-	degradation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43458]		hydrogen sulfide ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43458]	acid phosphatase	-	3.1.3.2
[Ref.: #43458]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43458]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #43458]	alpha-galactosidase	-	3.2.1.22
[Ref.: #43458]	alpha-glucosidase	-	3.2.1.20
[Ref.: #43458]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43458]	beta-D-fucosidase	-	3.2.1.38
[Ref.: #43458]	beta-galactosidase	-	3.2.1.23
[Ref.: #43458]	beta-glucosidase	-	3.2.1.21
[Ref.: #43458]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43458]	cystine arylamidase	-	3.4.11.3
[Ref.: #43458]	esterase (C 4)	+
[Ref.: #43458]	esterase Lipase (C 8)	-
[Ref.: #43458]	leucine arylamidase	-	3.4.11.1
[Ref.: #43458]	lipase (C 14)	-
[Ref.: #43458]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43458]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43458]	trypsin	-	3.4.21.4
[Ref.: #43458]	urease	+	3.5.1.5
[Ref.: #43458]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

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Fatty acid	Percentage
C15:0 iso	26.4
C15:0 anteiso	23.2
C17:0 iso	15.9
C17:0 anteiso	13.4
C16:0	5.7
C16:0 iso	5.1
C18:0	3.4
C18:1ω7c / C18:1ω6c	2.3
C15:0 iso 3OH	2
C14:0	1
C14:0 iso	0.8

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43458]	Sample type/isolated from	sediment sample collected from Jinze hot spring
[Ref.: #43458]	Geographic location (country and/or sea, region)	Tengchong county, Yunnan province
[Ref.: #43458]	Country	China
[Ref.: #43458]	Country ISO 3 Code	CHN
[Ref.: #43458]	Continent	Asia
[Ref.: #43458]	Geographic location	25.4330°/98.4500°

[Ref.: #67770]	Sample type/isolated from	Sediment from Jinze hot spring in Tengchong County
[Ref.: #67770]	Geographic location (country and/or sea, region)	Yunnan Province, south-west China
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Condition	#Thermophilic (>45°C)	-
#Environmental	#Aquatic	#Sediment
#Environmental	#Aquatic	#Thermal spring

What are isolation sources categories?

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Global distribution of 16S sequence KP232927 (>99% sequence identity) for Laceyella thermophila from Microbeatlas

Aquatic Samples	52
Soil Samples	89
Animal Samples	156
Plant Samples	33
Total Samples	330

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43458]	16S rRNA gene sequence	KP232927		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Laceyella thermophila YIM 79486	2784132171	draft	JGI IMG/M	1644115 tax ID	*

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GC-content

47.8 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

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Culture collection no.

CCTC AB 2015040, NBRC 110772, YIM 79486, DSM 46882, JCM 30657, BCRC 16987, CCTCC AB 2015040

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SI-ID 403481

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Laceyella thermophila sp. nov., a thermophilic bacterium isolated from a hot spring.	Ming H, Ji WL, Li S, Zhao ZL, Zhang LY, Meng XL, Zhou EM, Nie GX, Li WJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.002057	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43458

Hong Ming, Wei-Li Ji, Shuai Li, Zhuo-Li Zhao, Ling-Yu Zhang, Xiao-Lin Meng, En-Min Zhou, Guo-Xing Nie, Wen-Jun Li: Laceyella thermophila sp. nov., a thermophilic bacterium isolated from a hot spring. IJSEM 67: 2953 - 2958 2017 ( DOI 10.1099/ijsem.0.002057 , PubMed 28820123 )

#66517

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 46882

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#96777

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403481.1 )

* These data were automatically processed and therefore are not curated

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