Name: Thermoflexus hugenholtzii JAD2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140665

Type strain:

Species: Thermoflexus hugenholtzii

Strain Designation: JAD2

Culture col. no.: JCM 19131, CCTCC AB 2014030

Strain history: J. Dodsworth and B. P. Hedlund JAD2.

NCBI tax ID(s): 1495650 (species)

Section

Thermoflexus hugenholtzii JAD2 is a microaerophile, chemoheterotroph, thermophilic bacterium that forms circular colonies and was isolated from sediment of Great Boiling Spring in Nevada, USA.

colony-forming
filament-shaped
Gram-negative
chemoheterotroph
microaerophile
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Chloroflexota*
	Class	*Thermoflexia*
	Order	*Thermoflexales*
	Family	*Thermoflexaceae*
	Genus	*Thermoflexus*
	Species	**Thermoflexus hugenholtzii**
	Full Scientific Name (LPSN)	Thermoflexus hugenholtzii Dodsworth et al. 2014

Information on morphological and physiological properties Morphology

[Ref.: #43390]	Gram stain	negative

[Ref.: #43390]	Cell length	4 µm

[Ref.: #43390]	Cell width	0.3 µm

[Ref.: #43390]	Cell shape	filament-shaped

[Ref.: #43390]	Motility	no

[Ref.: #43390]	Colony size	0.2-0.3 mm
[Ref.: #43390]	Colony color	translucent, lack colour
[Ref.: #43390]	Colony shape	circular
[Ref.: #43390]	Cultivation medium used	GBS salts medium

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43390]

Culture medium

GBS salts medium

[Ref.: #43390]

Culture medium growth

		Temperature
[Ref.: #43390]	optimum	72.5-75 °C
[Ref.: #43390]	growth	67.5-75 °C
[Ref.: #67770]	growth	75 °C

[Ref.: #43390]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #43390]			optimum	6.75
[Ref.: #43390]			growth	6.5-7.75

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43390]	Oxygen tolerance	microaerophile
[Ref.: #43390]	Oxygen tolerance	facultative anaerobe

[Ref.: #43390]

Nutrition type

chemoheterotroph

[Ref.: #43390]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43390]		NaCl		optimum	0-100

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43390]	carboxymethylcellulose ChEBI	-	growth
[Ref.: #43390]	casamino acids	-	growth
[Ref.: #43390]	cellobiose ChEBI	-	growth
[Ref.: #43390]	cellulose ChEBI	-	growth
[Ref.: #43390]	D-fructose ChEBI	-	growth
[Ref.: #43390]	D-galactose ChEBI	-	growth
[Ref.: #43390]	D-glucose ChEBI	-	growth
[Ref.: #43390]	D-xylose ChEBI	-	growth
[Ref.: #43390]	glycine ChEBI	-	growth
[Ref.: #43390]	L-arginine ChEBI	-	growth
[Ref.: #43390]	L-aspartate ChEBI	-	growth
[Ref.: #43390]	L-glutamate ChEBI	-	growth
[Ref.: #43390]	L-histidine ChEBI	-	growth
[Ref.: #43390]	L-isoleucine ChEBI	-	growth
[Ref.: #43390]	L-leucine ChEBI	-	growth
[Ref.: #43390]	L-lysine ChEBI	-	growth
[Ref.: #43390]	L-methionine ChEBI	-	growth
[Ref.: #43390]	L-valine ChEBI	-	growth
[Ref.: #43390]	methane ChEBI	-	growth
[Ref.: #43390]	peptone	+	growth
[Ref.: #43390]	spectinomycin ChEBI	-	growth
[Ref.: #43390]	sucrose ChEBI	-	growth
[Ref.: #43390]	tryptone	+	growth
[Ref.: #43390]	yeast extract	+	growth
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43390]

Fatty acid	Percentage
C16:0	42
C19:0	16.1
C18:0	7.7
C20:0	7.2
C19:1	6.6
C18:1ω7t	3.9
C16:0 iso	3.6
C18:1ω9t	3.1
10-methyl C16:0	2.6
cyclopropane C19:0	2.3
C16:0 anteiso	1.8
C18:1	1.3
C17:0	1.1

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	C4 and CAM-carbon fixation	100	8 of 8
[Ref.: #66794]	cis-vaccenate biosynthesis	100	2 of 2
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	vitamin K metabolism	100	5 of 5
[Ref.: #66794]	formaldehyde oxidation	100	3 of 3
[Ref.: #66794]	palmitate biosynthesis	100	22 of 22
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	methylglyoxal degradation	100	5 of 5
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	valine metabolism	100	9 of 9
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	suberin monomers biosynthesis	100	2 of 2
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	molybdenum cofactor biosynthesis	88.89	8 of 9
[Ref.: #66794]	gluconeogenesis	87.5	7 of 8
[Ref.: #66794]	flavin biosynthesis	86.67	13 of 15
[Ref.: #66794]	citric acid cycle	85.71	12 of 14
[Ref.: #66794]	phenylalanine metabolism	84.62	11 of 13
[Ref.: #66794]	threonine metabolism	80	8 of 10
[Ref.: #66794]	3-chlorocatechol degradation	80	4 of 5
[Ref.: #66794]	starch degradation	80	8 of 10
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	phenylacetate degradation (aerobic)	80	4 of 5
[Ref.: #66794]	factor 420 biosynthesis	80	4 of 5
[Ref.: #66794]	glycine betaine biosynthesis	80	4 of 5
[Ref.: #66794]	propionate fermentation	80	8 of 10
[Ref.: #66794]	ethylmalonyl-CoA pathway	80	4 of 5
[Ref.: #66794]	glycogen metabolism	80	4 of 5
[Ref.: #66794]	photosynthesis	78.57	11 of 14
[Ref.: #66794]	d-mannose degradation	77.78	7 of 9
[Ref.: #66794]	CO2 fixation in Crenarchaeota	77.78	7 of 9
[Ref.: #66794]	serine metabolism	77.78	7 of 9
[Ref.: #66794]	aspartate and asparagine metabolism	77.78	7 of 9
[Ref.: #66794]	leucine metabolism	76.92	10 of 13
[Ref.: #66794]	sulfopterin metabolism	75	3 of 4
[Ref.: #66794]	toluene degradation	75	3 of 4
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	glutamate and glutamine metabolism	75	21 of 28
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	NAD metabolism	72.22	13 of 18
[Ref.: #66794]	reductive acetyl coenzyme A pathway	71.43	5 of 7
[Ref.: #66794]	propanol degradation	71.43	5 of 7
[Ref.: #66794]	tetrahydrofolate metabolism	71.43	10 of 14
[Ref.: #66794]	glycolysis	70.59	12 of 17
[Ref.: #66794]	purine metabolism	70.21	66 of 94
[Ref.: #66794]	non-pathway related	68.42	26 of 38
[Ref.: #66794]	L-lactaldehyde degradation	66.67	2 of 3
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	heme metabolism	64.29	9 of 14
[Ref.: #66794]	metabolism of disaccharids	63.64	7 of 11
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
[Ref.: #66794]	isoleucine metabolism	62.5	5 of 8
[Ref.: #66794]	isoprenoid biosynthesis	61.54	16 of 26
[Ref.: #66794]	tryptophan metabolism	60.53	23 of 38
[Ref.: #66794]	Entner Doudoroff pathway	60	6 of 10
[Ref.: #66794]	hydrogen production	60	3 of 5
[Ref.: #66794]	myo-inositol biosynthesis	60	6 of 10
[Ref.: #66794]	carotenoid biosynthesis	59.09	13 of 22
[Ref.: #66794]	ubiquinone biosynthesis	57.14	4 of 7
[Ref.: #66794]	degradation of sugar alcohols	56.25	9 of 16
[Ref.: #66794]	pyrimidine metabolism	55.56	25 of 45
[Ref.: #66794]	alanine metabolism	55.17	16 of 29
[Ref.: #66794]	histidine metabolism	55.17	16 of 29
[Ref.: #66794]	phenol degradation	55	11 of 20
[Ref.: #66794]	lipid metabolism	54.84	17 of 31
[Ref.: #66794]	pentose phosphate pathway	54.55	6 of 11
[Ref.: #66794]	vitamin E metabolism	50	2 of 4
[Ref.: #66794]	dTDPLrhamnose biosynthesis	50	4 of 8
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	arginine metabolism	50	12 of 24
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	50	6 of 12
[Ref.: #66794]	cyclohexanol degradation	50	2 of 4
[Ref.: #66794]	quinate degradation	50	1 of 2
[Ref.: #66794]	selenocysteine biosynthesis	50	3 of 6
[Ref.: #66794]	phenylmercury acetate degradation	50	1 of 2
[Ref.: #66794]	glycolate and glyoxylate degradation	50	3 of 6
[Ref.: #66794]	CMP-KDO biosynthesis	50	2 of 4
[Ref.: #66794]	glycine metabolism	50	5 of 10
[Ref.: #66794]	aminopropanol phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	pantothenate biosynthesis	50	3 of 6
[Ref.: #66794]	bile acid biosynthesis, neutral pathway	47.06	8 of 17
[Ref.: #66794]	methionine metabolism	46.15	12 of 26
[Ref.: #66794]	sulfate reduction	46.15	6 of 13
[Ref.: #66794]	proline metabolism	45.45	5 of 11
[Ref.: #66794]	degradation of hexoses	44.44	8 of 18
[Ref.: #66794]	daunorubicin biosynthesis	44.44	4 of 9
[Ref.: #66794]	cysteine metabolism	44.44	8 of 18
[Ref.: #66794]	tyrosine metabolism	42.86	6 of 14
[Ref.: #66794]	oxidative phosphorylation	42.86	39 of 91
[Ref.: #66794]	vitamin B12 metabolism	41.18	14 of 34
[Ref.: #66794]	lysine metabolism	40.48	17 of 42
[Ref.: #66794]	4-hydroxyphenylacetate degradation	40	4 of 10
[Ref.: #66794]	metabolism of amino sugars and derivatives	40	2 of 5
[Ref.: #66794]	coenzyme M biosynthesis	40	4 of 10
[Ref.: #66794]	arachidonate biosynthesis	40	2 of 5
[Ref.: #66794]	lipoate biosynthesis	40	2 of 5
[Ref.: #66794]	cellulose degradation	40	2 of 5
[Ref.: #66794]	vitamin B1 metabolism	38.46	5 of 13
[Ref.: #66794]	androgen and estrogen metabolism	37.5	6 of 16
[Ref.: #66794]	ketogluconate metabolism	37.5	3 of 8
[Ref.: #66794]	carnitine metabolism	37.5	3 of 8
[Ref.: #66794]	ascorbate metabolism	36.36	8 of 22
[Ref.: #66794]	vitamin B6 metabolism	36.36	4 of 11
[Ref.: #66794]	d-xylose degradation	36.36	4 of 11
[Ref.: #66794]	dolichyl-diphosphooligosaccharide biosynthesis	36.36	4 of 11
[Ref.: #66794]	degradation of pentoses	35.71	10 of 28
[Ref.: #66794]	lipid A biosynthesis	33.33	3 of 9
[Ref.: #66794]	cyanate degradation	33.33	1 of 3
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	4-hydroxymandelate degradation	33.33	3 of 9
[Ref.: #66794]	sphingosine metabolism	33.33	2 of 6
[Ref.: #66794]	nitrate assimilation	33.33	3 of 9
[Ref.: #66794]	enterobactin biosynthesis	33.33	1 of 3
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	30.77	4 of 13
[Ref.: #66794]	urea cycle	30.77	4 of 13
[Ref.: #66794]	benzoyl-CoA degradation	28.57	2 of 7
[Ref.: #66794]	cardiolipin biosynthesis	28.57	2 of 7
[Ref.: #66794]	aclacinomycin biosynthesis	28.57	2 of 7
[Ref.: #66794]	arachidonic acid metabolism	27.78	5 of 18
[Ref.: #66794]	cholesterol biosynthesis	27.27	3 of 11
[Ref.: #66794]	3-phenylpropionate degradation	26.67	4 of 15
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	biotin biosynthesis	25	1 of 4
[Ref.: #66794]	butanoate fermentation	25	1 of 4
[Ref.: #66794]	alginate biosynthesis	25	1 of 4
[Ref.: #66794]	glutathione metabolism	21.43	3 of 14
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43390]	Sample type/isolated from	sediment of Great Boiling Spring in Nevada, USA
[Ref.: #43390]	Geographic location (country and/or sea, region)	Great boiling spring, Nevada
[Ref.: #43390]	Country	USA
[Ref.: #43390]	Country ISO 3 Code	USA
[Ref.: #43390]	Continent	North America
[Ref.: #43390]	Geographic location	40.0000°/-119.0000°

[Ref.: #67770]	Sample type/isolated from	Sediment of Great Boiling Spring
[Ref.: #67770]	Geographic location (country and/or sea, region)	NV
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Spring
#Environmental	#Aquatic	#Sediment

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KC526151 (>99% sequence identity) for Thermoflexus hugenholtzii subclade from Microbeatlas

Aquatic Samples	120
Soil Samples	4
Animal Samples	27
Plant Samples
Total Samples	151

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43390]	16S rRNA gene sequence	KC526151		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Thermoflexus hugenholtzii JAD2	GCA_900187885	scaffold	GenBank	877466 tax ID
[Ref.: #66792]	Thermoflexus hugenholtzii JAD2	2140918011	draft	JGI IMG/M	877466 tax ID	*

[Ref.: #43390]

GC-content

69.3 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #43390]

Culture collection no.

JCM 19131, CCTCC AB 2014030

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Thermoflexus hugenholtzii gen. nov., sp. nov., a thermophilic, microaerophilic, filamentous bacterium representing a novel class in the Chloroflexi, Thermoflexia classis nov., and description of Thermoflexaceae fam. nov. and Thermoflexales ord. nov.	Dodsworth JA, Gevorkian J, Despujos F, Cole JK, Murugapiran SK, Ming H, Li WJ, Zhang G, Dohnalkova A, Hedlund BP	Int J Syst Evol Microbiol	10.1099/ijs.0.055855-0	2014	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43390

Jeremy A. Dodsworth, Jonathan Gevorkian, Fairuz Despujos, Jessica K. Cole, Senthil K. Murugapiran, Hong Ming, Wen-Jun Li, Gengxin Zhang, Alice Dohnalkova, Brian P. Hedlund: Thermoflexus hugenholtzii gen. nov., sp. nov., a thermophilic, microaerophilic, filamentous bacterium representing a novel class in the Chloroflexi, Thermoflexia classis nov., and description of Thermoflexaceae fam. nov. and Thermoflexales ord. nov.. IJSEM 64: 2119 - 2127 2014 ( DOI 10.1099/ijs.0.055855-0 , PubMed 24676733 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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