Cite this strain

"Caldicellulosiruptor obsidiansis" (ATCC BAA-2073, OB47, JCM 16842)

BacDive ID 140656

Type strain

Strain Designation OB47

Culture col. no. ATCC BAA-2073 OB47 JCM 16842

NCBI tax ID(s) 717609

Special Collections JCM Literature strains

History J. G. Elkins OB47.

Links

"Caldicellulosiruptor obsidiansis" OB47 is an obligate anaerobe, chemoorganoheterotroph, Gram-positive bacterium that forms circular colonies and was isolated from Sample consisting of water, sediment and grass taken from a collection site at the edge of Obsidian Pool .

Gram-positive rod-shaped colony-forming obligate anaerobe chemoorganoheterotroph genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 131114

ATCC BAA-2073,JCM 16842

@ref 20215

Last LPSN update 2026-02-09 11:33:40

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Caldicellulosiruptorales

Family Caldicellulosiruptoraceae

Genus Caldicellulosiruptor

Species "Caldicellulosiruptor obsidiansis"

Full scientific name Caldicellulosiruptor obsidiansis Hamilton-Brehm et al. 2010

Synonyms (1)

"Anaerocellum obsidiansis"

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
43401	positive	2 µm	0.2 µm	rod-shaped

Colony morphology

@ref	Colony color	Colony shape	Medium used
43401	opal	circular	gelrite plate with 0.5%(w/v) cellobiose and yeast extract

Culture and growth conditions

Culture medium

@ref	Name	Growth
43401	gelrite plate with 0.5%(w/v) cellobiose and yeast extract

Culture temp

@ref	Growth	Type	Temperature (°C)
43401	positive	optimum	78
43401	positive	growth	55-85
67770	positive	growth	75

Culture pH

@ref	Ability	Type	PH
43401	positive	optimum	7
43401	positive	growth	5-8

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
43401	obligate anaerobe
125438	anaerobe	97.359
125439	anaerobe	95.5

Nutrition type

43401

Typechemoorganoheterotroph

Spore formation

43401

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
43401	NaCl	positive	growth	0.5 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43401	30089 ChEBI	acetate	-	growth
43401	27569 ChEBI	arabinogalactan	+	growth
43401	22599 ChEBI	arabinose	+	growth
43401	85146 ChEBI	carboxymethylcellulose	-	growth
43401		casein	-	growth
43401	17057 ChEBI	cellobiose	+	growth
43401	62968 ChEBI	cellulose	+	growth
43401	62968 ChEBI	cellulose	+	degradation
43401	33403 ChEBI	elemental sulfur	-	assimilation
43401	28757 ChEBI	fructose	+	growth
43401	33984 ChEBI	fucose	-	growth
43401	28260 ChEBI	galactose	+	growth
43401	85248 ChEBI	gellan gum	-	growth
43401	17234 ChEBI	glucose	+	growth
43401	17754 ChEBI	glycerol	-	growth
43401	17716 ChEBI	lactose	+	growth
43401	6457 ChEBI	lignin	-	growth
43401	17306 ChEBI	maltose	+	growth
43401	29864 ChEBI	mannitol	-	growth
43401	37684 ChEBI	mannose	+	growth
43401	17632 ChEBI	nitrate	-	assimilation
43401	17309 ChEBI	pectin	+	growth
43401	33942 ChEBI	ribose	-	growth
43401	30911 ChEBI	sorbitol	-	growth
43401	28017 ChEBI	starch	+	growth
43401	17992 ChEBI	sucrose	+	growth
43401	16189 ChEBI	sulfate	-	assimilation
43401	16094 ChEBI	thiosulfate	-	assimilation
43401	27082 ChEBI	trehalose	+	growth
43401	37166 ChEBI	xylan	+	growth
43401	18222 ChEBI	xylose	+	growth
43401		yeast extract (0.01 %, w/v)	+	growth

Metabolite production

@ref	Chebi-ID	Metabolite
43401	30089 ChEBI	acetate
43401	16526 ChEBI	carbon dioxide
43401	18276 ChEBI	dihydrogen
43401	24996 ChEBI	lactate

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Environmental	#Aquatic
#Condition	#Thermophilic (>45°C)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
43401	Sample consisting of water, sediment and grass taken from a collection site at the edge of Obsidian Pool (66°C, pH 5)	Yellowstone National Park	USA	USA	North America
67770	Obsidian Pool	Yellowstone National Park, WY	USA	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence GQ338764 (>99% sequence identity) for Caldicellulosiruptor obsidiansis subclade from Microbeatlas

Aquatic Samples	7
Soil Samples
Animal Samples
Plant Samples	1
Total Samples	8

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM14521v1 assembly for Caldicellulosiruptor obsidiansis OB47	complete	GCA_000145215	608506.3	648028013	608506	99.57

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
67770	Caldicellulosiruptor obsidiansis strain OB47 16S ribosomal RNA gene, partial sequence	GQ338764	1466		608506

GC content

@ref	GC-content (mol%)	Method
43401	35.2	sequence analysis
67770	35

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Caldicellulosiruptor obsidiansis OB47
PATRIC: 608506.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	83.40	no
125439	motility	BacteriaNetⓘ	yes	84.60	no
125439	gram_stain	BacteriaNetⓘ	variable	82.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	95.50	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Caldicellulosiruptor obsidiansis OB47 OB47
NCBI: GCA_000145215.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	71.16	yes
125438	anaerobic	anaerobicⓘ	yes	97.36	yes
125438	aerobic	aerobicⓘ	no	97.93	no
125438	spore-forming	spore-formingⓘ	no	63.90	yes
125438	thermophilic	thermophileⓘ	yes	85.69	yes
125438	flagellated	motile2+ⓘ	yes	74.21	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Evaluation of the bioconversion of genetically modified switchgrass using simultaneous saccharification and fermentation and a consolidated bioprocessing approach.	Yee KL, Rodriguez M, Tschaplinski TJ, Engle NL, Martin MZ, Fu C, Wang ZY, Hamilton-Brehm SD, Mielenz JR.	Biotechnol Biofuels	10.1186/1754-6834-5-81	2012
	Overcoming restriction as a barrier to DNA transformation in Caldicellulosiruptor species results in efficient marker replacement.	Chung D, Farkas J, Westpheling J.	Biotechnol Biofuels	10.1186/1754-6834-6-82	2013
Genetics	Complete genome sequence of the cellulolytic thermophile Caldicellulosiruptor obsidiansis OB47T.	Elkins JG, Lochner A, Hamilton-Brehm SD, Davenport KW, Podar M, Brown SD, Land ML, Hauser LJ, Klingeman DM, Raman B, Goodwin LA, Tapia R, Meincke LJ, Detter JC, Bruce DC, Han CS, Palumbo AV, Cottingham RW, Keller M, Graham DE	J Bacteriol	10.1128/JB.00950-10	2010
Enzymology	Caldicellulosiruptor obsidiansis sp. nov., an anaerobic, extremely thermophilic, cellulolytic bacterium isolated from Obsidian Pool, Yellowstone National Park.	Hamilton-Brehm SD, Mosher JJ, Vishnivetskaya T, Podar M, Carroll S, Allman S, Phelps TJ, Keller M, Elkins JG.	Appl Environ Microbiol	10.1128/aem.01903-09	2010

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#43401	Scott D. Hamilton-Brehm, Jennifer J. Mosher, Tatiana Vishnivetskaya, Mircea Podar, Sue Carroll, Steve Allman, Tommy J. Phelps, Martin Keller, and James G. Elkins: Caldicellulosiruptor obsidiansis sp. nov., an Anaerobic, Extremely Thermophilic, Cellulolytic Bacterium Isolated from Obsidian Pool, Yellowstone National Park. Appl. Environ. Microbiol. 76(4): 1014 - 1020 2010 ( DOI 10.1128/AEM.01903-09 , PubMed 20023107 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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"Caldicellulosiruptor obsidiansis" (ATCC BAA-2073, OB47, JCM 16842)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Nutrition type

Spore formation

Halophily

Metabolite utilization

Metabolite production

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_000145215

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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