Name: Azospirillum humicireducens SgZ-5
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140653

Type strain:

Species: Azospirillum humicireducens

Strain Designation: SgZ-5

Culture col. no.: CCTCC AB 2012021, KACC 16605

NCBI tax ID(s): 1226968 (species)

Other strains from Azospirillum humicireducens

A. humicireducens

Section

Azospirillum humicireducens SgZ-5 is a facultative anaerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from biofilm on a microbial fuel cell in a laboratory of the Guangdong Institute of Eco-Environmental and Soil Sciences that was previously inoculated with 2 g paddy soil and 10 mL sodium acetate culture medium solution.

colony-forming
Gram-negative
motile
spiral-shaped
facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Rhodospirillales*
	Family	*Azospirillaceae*
	Genus	*Azospirillum*
	Species	**Azospirillum humicireducens**
	Full Scientific Name (LPSN)	Azospirillum humicireducens Zhou et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #27215]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.971

[Ref.: #27215]	Cell length	2.5-5.5 µm

[Ref.: #27215]	Cell width	1.2-1.8 µm

[Ref.: #27215]	Cell shape	spiral-shaped

[Ref.: #27215]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %97.536

[Ref.: #27215]	Flagellum arrangement	monotrichous, polar

[Ref.: #27215]	Colony size	1-1.5 mm
[Ref.: #27215]	Colony color	cream-coloured
[Ref.: #27215]	Colony shape	circular
[Ref.: #27215]	Incubation period	1 day
[Ref.: #27215]	Cultivation medium used	Nutrient agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #27215]

Culture medium

Nutrient agar (NA)

[Ref.: #27215]

Culture medium growth

[Ref.: #27215]

Culture medium

MacConkey Agar

[Ref.: #27215]

Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #27215]			optimum	30 °C
[Ref.: #27215]			growth	25-37 °C

[Ref.: #27215]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #27215]			optimum	7.2
[Ref.: #27215]			growth	5.5-8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #27215]

Oxygen tolerance

facultative anaerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %99
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.978

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #27215]		NaCl		growth	0-1	%
[Ref.: #27215]		NaCl		optimum	1	%

[Ref.: #27215]	Observation	production of intracellular poly-beta-hydroxybutyrate granules
[Ref.: #27215]	Observation	Denitrification

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #27215]	2-oxogluconate	+	carbon source
[Ref.: #27215]	3-hydroxybenzoate ChEBI	-	carbon source
[Ref.: #27215]	3-hydroxybutyrate ChEBI	+	carbon source
[Ref.: #27215]	4-hydroxybutyrate ChEBI	+	carbon source
[Ref.: #27215]	5-dehydro-D-gluconate ChEBI	-	carbon source
[Ref.: #27215]	adipate ChEBI	+	carbon source
[Ref.: #27215]	amygdalin ChEBI	+/-	builds acid from
[Ref.: #27215]	biotin ChEBI	-	required for growth
[Ref.: #27215]	casein	+	hydrolysis
[Ref.: #27215]	cellulose ChEBI	-	hydrolysis
[Ref.: #27215]	citrate ChEBI	+	carbon source
[Ref.: #27215]	D-glucose ChEBI	+	builds acid from
[Ref.: #27215]	D-glucose ChEBI	+	carbon source
[Ref.: #27215]	D-mannitol ChEBI	-	builds acid from
[Ref.: #27215]	D-mannitol ChEBI	+	carbon source
[Ref.: #27215]	D-mannose ChEBI	+/-	carbon source
[Ref.: #27215]	D-ribose ChEBI	+	carbon source
[Ref.: #27215]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #27215]	D-sorbitol ChEBI	+	carbon source
[Ref.: #27215]	decanoate ChEBI	+	carbon source
[Ref.: #27215]	dinitrogen ChEBI	+	nitrogen source
[Ref.: #27215]	esculin ChEBI	+	hydrolysis
[Ref.: #27215]	gelatin ChEBI	-	hydrolysis
[Ref.: #27215]	glucose ChEBI	+	aerobic growth
[Ref.: #27215]	glucose ChEBI	+	anaerobic growth
[Ref.: #27215]	glycogen ChEBI	+	carbon source
[Ref.: #27215]	itaconate ChEBI	-	carbon source
[Ref.: #27215]	L-arabinose ChEBI	+	builds acid from
[Ref.: #27215]	L-arabinose ChEBI	+	carbon source
[Ref.: #27215]	L-fucose ChEBI	+	carbon source
[Ref.: #27215]	L-histidine ChEBI	+	carbon source
[Ref.: #27215]	L-proline ChEBI	+	carbon source
[Ref.: #27215]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #27215]	L-rhamnose ChEBI	+	carbon source
[Ref.: #27215]	lecithin ChEBI	-	hydrolysis
[Ref.: #27215]	malate ChEBI	+	carbon source
[Ref.: #27215]	malonate ChEBI	+	carbon source
[Ref.: #27215]	maltose ChEBI	+	carbon source
[Ref.: #27215]	melibiose ChEBI	-	builds acid from
[Ref.: #27215]	melibiose ChEBI	+	carbon source
[Ref.: #27215]	myo-inositol ChEBI	-	builds acid from
[Ref.: #27215]	myo-inositol ChEBI	+	carbon source
[Ref.: #27215]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #27215]	nitrate ChEBI	+	reduction
[Ref.: #27215]	nitrite ChEBI	-	reduction
[Ref.: #27215]	phenylacetate ChEBI	-	carbon source
[Ref.: #27215]	propionate ChEBI	-	carbon source
[Ref.: #27215]	starch ChEBI	+	hydrolysis
[Ref.: #27215]	suberic acid ChEBI	+	carbon source
[Ref.: #27215]	sucrose ChEBI	-	builds acid from
[Ref.: #27215]	sucrose ChEBI	+	carbon source
[Ref.: #27215]	tween 20 ChEBI	+	hydrolysis
[Ref.: #27215]	tween 80 ChEBI	-	hydrolysis
[Ref.: #27215]	valerate ChEBI	-	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #27215]	acetoin ChEBI	no
[Ref.: #27215]	hydrogen sulfide ChEBI	no
[Ref.: #27215]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #27215]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #27215]	arginine dihydrolase	-	3.5.3.6
[Ref.: #27215]	beta-galactosidase	+	3.2.1.23
[Ref.: #27215]	catalase	+	1.11.1.6
[Ref.: #27215]	cytochrome oxidase	+	1.9.3.1
[Ref.: #27215]	DNase	-
[Ref.: #27215]	lysine decarboxylase	-	4.1.1.18
[Ref.: #27215]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #27215]	tryptophan deaminase	+/-	4.1.99.1
[Ref.: #27215]	urease	+/-	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #27215]	Sample type/isolated from	biofilm on a microbial fuel cell in a laboratory of the Guangdong Institute of Eco-Environmental and Soil Sciences that was previously inoculated with 2 g paddy soil and 10 mL sodium acetate culture medium solution
[Ref.: #27215]	Geographic location (country and/or sea, region)	China
[Ref.: #27215]	Country	China
[Ref.: #27215]	Country ISO 3 Code	CHN
[Ref.: #27215]	Continent	Asia
[Ref.: #27215]	Enrichment culture	100 mL sterilized mineral salts medium
[Ref.: #27215]	Enrichment culture composition	2.5 g/L NaHCO3, 0.68 g/L NaH2Po4, 0.25 g/L NH4CL, 0.1 g/L KCl, 1% v/v trace mineral and vitamin solution (Lovley and Phillips, 1988) supplemented with sodium acetate 10 mM and AQDS 1mM
[Ref.: #27215]	Isolation/enrichment procedure	piece of anode was transferred into medium
* marker position based on {}

Isolation sources categories

#Engineered	#Bioreactor	-
#Environmental	#Biofilm	-
#Engineered	#Laboratory	-

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #27215]	16S-RNA gene	JX274435		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Azospirillum humicireducens SgZ-5	GCA_001639105	chromosome	GenBank	1226968 tax ID	*
[Ref.: #66792]	Azospirillum humicireducens strain SgZ-5	1226968.3	complete	PATRIC	1226968 tax ID	*
[Ref.: #66792]	Azospirillum humicireducens SgZ-5	2687453243	complete	JGI IMG/M	1226968 tax ID	*

[Ref.: #27215]

GC-content

67.7 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Azospirillum humicireducens strain SgZ-5 PATRIC: 1226968.3
[Ref.: #69481]	spore-forming	no	99	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Azospirillum humicireducens strain SgZ-5 PATRIC: 1226968.3
[Ref.: #69480]	motile	yes	90.477	yes
[Ref.: #69480]	gram-positive	no	98.488	yes
[Ref.: #69480]	anaerobic	no	95.213	yes
[Ref.: #69480]	aerobic	yes	57.47	no
[Ref.: #69480]	halophile	no	91.697	no
[Ref.: #69480]	spore-forming	no	93.126	no
[Ref.: #69480]	thermophile	no	89.341	yes
[Ref.: #69480]	glucose-util	yes	87.851	yes
[Ref.: #69480]	flagellated	yes	61.03	no
[Ref.: #69480]	glucose-ferment	no	87.348	no

Availability in culture collections External links

[Ref.: #27215]

Culture collection no.

CCTCC AB 2012021, KACC 16605

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Azospirillum humicireducens sp. nov., a nitrogen-fixing bacterium isolated from a microbial fuel cell.	Zhou S, Han L, Wang Y, Yang G, Zhuang L, Hu P	Int J Syst Evol Microbiol	10.1099/ijs.0.046813-0	2012	*
Genetics	Complete genome sequence of the nitrogen-fixing bacterium Azospirillum humicireducens type strain SgZ-5(T).	Yu Z, Yang G, Liu X, Wang Y, Zhuang L, Zhou S	Stand Genomic Sci	10.1186/s40793-018-0322-2	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#27215

IJSEM 2618 2013 ( DOI 10.1099/ijs.0.046813-0 , PubMed 23264502 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Azospirillum humicireducens strain SgZ-5

Azospirillum humicireducens strain SgZ-5

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