Halorubrum pallidum PJ61 | Type strain | CGMCC 1.15212, JCM 30955

Strain identifier

BacDive ID: 140642

Type strain:

Species: Halorubrum pallidum

Strain Designation: PJ61

Culture col. no.: CGMCC 1.15212, JCM 30955

Section

Name and taxonomic classification
Morphology and physiology
Culture and growth conditions
Isolation, sampling and environmental information
Molecular biology
Strain availability

External links

Halorubrum pallidum PJ61 is an aerobe, mesophilic, gram-negative archaeon that forms circular colonies and was isolated from subterranean rock salt.

colony-forming
gram-negative
square-shaped
aerobe
mesophilic
16S sequence
Archaea

Information on the name and the taxonomic classification.Name and taxonomic classification

	Last LPSN update	10-09-2020 (DD-MM-YYYY)
	Domain	Archaea
	Phylum	Euryarchaeota
	Class	Halobacteria
	Order	Haloferacales
	Family	Halorubraceae
	Genus	Halorubrum
	Species	Halorubrum pallidum
	Full Scientific Name (PNU)	Halorubrum pallidum Chen et al. 2016

Information on morphological and physiological propertiesMorphology and physiology

[Ref.: #43078]	Gram stain	negative
[Ref.: #43078]	Cell length	1.0-1.2 µm
[Ref.: #43078]	Cell width	0.5-0.8 µm
[Ref.: #43078]	Cell shape	sphere-shaped
[Ref.: #43078]	Motility	no

[Ref.: #43078]	Cell shape	oval-shaped

[Ref.: #43078]	Colony size	1.0-2.0 mm
[Ref.: #43078]	Colony color	pale
[Ref.: #43078]	Colony shape	circular
[Ref.: #43078]	Cultivation medium used	AS-158 agar

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #43078]		catalase	+	1.11.1.6
[Ref.: #43078]		cytochrome oxidase	+	1.9.3.1

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43078]		NaCl		growth	2.6-5.1	M
[Ref.: #43078]		NaCl		optimum	3.4	M

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #43078]	ampicillin ChEBI				yes	10	µg (disc)
[Ref.: #43078]	bacitracin ChEBI				yes	0.04	Unit
[Ref.: #43078]	chloramphenicol ChEBI				yes	30	µg (disc)
[Ref.: #43078]	ciprofloxacin ChEBI				yes	5	µg (disc)
[Ref.: #43078]	erythromycin ChEBI				yes	15	µg (disc)
[Ref.: #43078]	kanamycin ChEBI				yes	10	µg (disc)
[Ref.: #43078]	neomycin ChEBI	yes	30	µg (disc)
[Ref.: #43078]	norfloxacin ChEBI				yes	10	µg (disc)
[Ref.: #43078]	novobiocin ChEBI	yes	30	µg (disc)
[Ref.: #43078]	penicillin g ChEBI				yes	10	Unit
[Ref.: #43078]	rifampicin ChEBI				yes	5	µg (disc)
[Ref.: #43078]	streptomycin ChEBI				yes	10	µg (disc)
[Ref.: #43078]	tetracycline ChEBI				yes	30	µg (disc)
[Ref.: #43078]	vancomycin ChEBI				yes	30	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43078]		hydrogen sulfide ChEBI	yes
[Ref.: #43078]		indole ChEBI	no

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43078]	casein	-	hydrolysis
[Ref.: #43078]	citrate ChEBI	-	carbon source
[Ref.: #43078]	D-fructose ChEBI	+	carbon source
[Ref.: #43078]	D-fructose ChEBI	+	builds acid from
[Ref.: #43078]	D-galactose ChEBI	+	carbon source
[Ref.: #43078]	D-galactose ChEBI	+	builds acid from
[Ref.: #43078]	D-glucose ChEBI	+	carbon source
[Ref.: #43078]	D-glucose ChEBI	+	builds acid from
[Ref.: #43078]	D-mannitol ChEBI	-	carbon source
[Ref.: #43078]	D-mannose ChEBI	+	carbon source
[Ref.: #43078]	D-mannose ChEBI	+	builds acid from
[Ref.: #43078]	D-ribose ChEBI	-	carbon source
[Ref.: #43078]	D-sorbitol ChEBI	-	carbon source
[Ref.: #43078]	D-sorbose ChEBI	-	carbon source
[Ref.: #43078]	D-sorbose ChEBI	+	builds acid from
[Ref.: #43078]	D-xylose ChEBI	+	carbon source
[Ref.: #43078]	D-xylose ChEBI	+	builds acid from
[Ref.: #43078]	fumarate ChEBI	+	carbon source
[Ref.: #43078]	gelatin ChEBI	-	hydrolysis
[Ref.: #43078]	glycerol ChEBI	+	carbon source
[Ref.: #43078]	glycine ChEBI	-	carbon source
[Ref.: #43078]	L-alanine ChEBI	-	carbon source
[Ref.: #43078]	L-arginine ChEBI	-	carbon source
[Ref.: #43078]	L-aspartate ChEBI	-	carbon source
[Ref.: #43078]	L-glutamate ChEBI	+	carbon source
[Ref.: #43078]	L-lysine ChEBI	-	carbon source
[Ref.: #43078]	L-ornithine ChEBI	-	carbon source
[Ref.: #43078]	lactate ChEBI	+	carbon source
[Ref.: #43078]	lactose ChEBI	-	carbon source
[Ref.: #43078]	magnesium(2+) ChEBI	+	required for growth
[Ref.: #43078]	malate ChEBI	+	carbon source
[Ref.: #43078]	maltose ChEBI	+	carbon source
[Ref.: #43078]	nitrate ChEBI	-	reduction
[Ref.: #43078]	nitrite ChEBI	-	reduction
[Ref.: #43078]	pyruvate ChEBI	+	carbon source
[Ref.: #43078]	starch ChEBI	-	hydrolysis
[Ref.: #43078]	starch ChEBI	-	carbon source
[Ref.: #43078]	succinate ChEBI	+	carbon source
[Ref.: #43078]	sucrose ChEBI	-	carbon source
[Ref.: #43078]	tween 20 ChEBI	-	hydrolysis
[Ref.: #43078]	tween 40 ChEBI	-	hydrolysis
[Ref.: #43078]	tween 60 ChEBI	-	hydrolysis
[Ref.: #43078]	tween 80 ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

[Ref.: #43078]

Observation

Cell lysis in distilled water

[Ref.: #43078]

Oxygen tolerance

aerobe

Information on culture and growth conditionsCulture and growth conditions

[Ref.: #43078]

Culture medium

AS-158 agar

[Ref.: #43078]

Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #43078]			optimum	38 ̊C
[Ref.: #43078]			growth	25-45 ̊C

[Ref.: #43078]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #43078]			optimum	8
[Ref.: #43078]			growth	7.5-8.5

Information on isolation source, the sampling and environmental conditionsIsolation, sampling and environmental information

[Ref.: #43078]

Sample type/isolated from

subterranean rock salt

[Ref.: #43078]

Geographic location (country and/or sea, region)

Yuanyongjing Salt Mine, Yunnan province, China

[Ref.: #43078]

Country

China

[Ref.: #43078]

Country ISO 3 Code

CHN

[Ref.: #43078]

Continent

Asia

[Ref.: #43078]

Geographic location

25.0000°/101.0000°

Isolation sources categories

#Condition	#Saline	-
#Environmental	#Terrestrial	#Geologic

What are isolation sources categories?

* marker position based on {}

Information on genomic background e.g. entries in nucleic sequence databassMolecular biology

[Ref.: #43078]

GC-content

65.1 mol%

thermal denaturation, midpoint method (Tm)

Sequence information:

	Sequence database	Sequence accession description	Sequence accession number	Sequence type	Sequence length (bp)	Associated NCBI tax ID
[Ref.: #43078]	GenBank/EMBL/DDBJ	16S rRNA gene sequences	KJ644242 ENA	16S	1471	1526114 tax ID
[Ref.: #43078]	GenBank/EMBL/DDBJ	rpoB gene sequences	KT803863 ENA		1830	1526114 tax ID
[Ref.: #43078]	GenBank/EMBL/DDBJ	ef-2 gene sequences	KT803860 ENA		1802	1526114 tax ID

Availability in culture collectionsStrain availability

[Ref.: #43078]

Culture collection no.

CGMCC 1.15212, JCM 30955

References

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#43078

Shaoxing Chen, Hong-Can Liu, Jian Zhou and Hua Xiang: Halorubrum pallidum sp. nov., an extremely halophilic archaeon isolated from a subterranean rock salt. IJSEM 66: 2980 - 2986 2016 ( DOI 10.1099/ijsem.0.001129 )

* These References are textmined

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