Name: Halorubrum pallidum PJ61
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140642

Type strain:

Species: Halorubrum pallidum

Strain Designation: PJ61

Culture col. no.: CGMCC 1.15212, JCM 30955

Strain history: H. Xiang; Inst. of Microbiol., CAS, China; PJ61.

NCBI tax ID(s): 1526114 (species)

Section

Halorubrum pallidum PJ61 is an aerobe, mesophilic, Gram-negative archaeon that forms circular colonies and was isolated from subterranean rock salt.

colony-forming
Gram-negative
sphere-shaped
aerobe
mesophilic
16S sequence
Archaea

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	*Halobacteria*
	Order	*Haloferacales*
	Family	*Halorubraceae*
	Genus	*Halorubrum*
	Species	**Halorubrum pallidum**
	Full Scientific Name (LPSN)	Halorubrum pallidum Chen et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43078]	Gram stain	negative

[Ref.: #43078]	Cell length	1.0-1.2 µm

[Ref.: #43078]	Cell width	0.5-0.8 µm

[Ref.: #43078]	Cell shape	sphere-shaped
[Ref.: #43078]	Cell shape	oval-shaped

[Ref.: #43078]	Motility	no

[Ref.: #43078]	Colony size	1.0-2.0 mm
[Ref.: #43078]	Colony color	pale
[Ref.: #43078]	Colony shape	circular
[Ref.: #43078]	Cultivation medium used	AS-158 agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43078]

Culture medium

AS-158 agar

[Ref.: #43078]

Culture medium growth

		Temperature
[Ref.: #43078]	optimum	38 °C
[Ref.: #43078]	growth	25-45 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #43078]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43078]			optimum	8
[Ref.: #43078]			growth	7.5-8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43078]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43078]		NaCl		growth	2.6-5.1	M
[Ref.: #43078]		NaCl		optimum	3.4	M

[Ref.: #43078]

Observation

Cell lysis in distilled water

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43078]	casein	-	hydrolysis
[Ref.: #43078]	citrate ChEBI	-	carbon source
[Ref.: #43078]	D-fructose ChEBI	+	carbon source
[Ref.: #43078]	D-fructose ChEBI	+	builds acid from
[Ref.: #43078]	D-galactose ChEBI	+	carbon source
[Ref.: #43078]	D-galactose ChEBI	+	builds acid from
[Ref.: #43078]	D-glucose ChEBI	+	carbon source
[Ref.: #43078]	D-glucose ChEBI	+	builds acid from
[Ref.: #43078]	D-mannitol ChEBI	-	carbon source
[Ref.: #43078]	D-mannose ChEBI	+	carbon source
[Ref.: #43078]	D-mannose ChEBI	+	builds acid from
[Ref.: #43078]	D-ribose ChEBI	-	carbon source
[Ref.: #43078]	D-sorbitol ChEBI	-	carbon source
[Ref.: #43078]	D-sorbose ChEBI	-	carbon source
[Ref.: #43078]	D-sorbose ChEBI	+	builds acid from
[Ref.: #43078]	D-xylose ChEBI	+	carbon source
[Ref.: #43078]	D-xylose ChEBI	+	builds acid from
[Ref.: #43078]	fumarate ChEBI	+	carbon source
[Ref.: #43078]	gelatin ChEBI	-	hydrolysis
[Ref.: #43078]	glycerol ChEBI	+	carbon source
[Ref.: #43078]	glycine ChEBI	-	carbon source
[Ref.: #43078]	L-alanine ChEBI	-	carbon source
[Ref.: #43078]	L-arginine ChEBI	-	carbon source
[Ref.: #43078]	L-aspartate ChEBI	-	carbon source
[Ref.: #43078]	L-glutamate ChEBI	+	carbon source
[Ref.: #43078]	L-lysine ChEBI	-	carbon source
[Ref.: #43078]	L-ornithine ChEBI	-	carbon source
[Ref.: #43078]	lactate ChEBI	+	carbon source
[Ref.: #43078]	lactose ChEBI	-	carbon source
[Ref.: #43078]	magnesium(2+) ChEBI	+	required for growth
[Ref.: #43078]	malate ChEBI	+	carbon source
[Ref.: #43078]	maltose ChEBI	+	carbon source
[Ref.: #43078]	nitrate ChEBI	-	reduction
[Ref.: #43078]	nitrite ChEBI	-	reduction
[Ref.: #43078]	pyruvate ChEBI	+	carbon source
[Ref.: #43078]	starch ChEBI	-	hydrolysis
[Ref.: #43078]	starch ChEBI	-	carbon source
[Ref.: #43078]	succinate ChEBI	+	carbon source
[Ref.: #43078]	sucrose ChEBI	-	carbon source
[Ref.: #43078]	tween 20 ChEBI	-	hydrolysis
[Ref.: #43078]	tween 40 ChEBI	-	hydrolysis
[Ref.: #43078]	tween 60 ChEBI	-	hydrolysis
[Ref.: #43078]	tween 80 ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #43078]	ampicillin ChEBI				yes	10	µg (disc)
[Ref.: #43078]	bacitracin ChEBI				yes	0.04	Unit
[Ref.: #43078]	chloramphenicol ChEBI				yes	30	µg (disc)
[Ref.: #43078]	ciprofloxacin ChEBI				yes	5	µg (disc)
[Ref.: #43078]	erythromycin ChEBI				yes	15	µg (disc)
[Ref.: #43078]	kanamycin ChEBI				yes	10	µg (disc)
[Ref.: #43078]	neomycin ChEBI	yes	30	µg (disc)
[Ref.: #43078]	norfloxacin ChEBI				yes	10	µg (disc)
[Ref.: #43078]	novobiocin ChEBI	yes	30	µg (disc)
[Ref.: #43078]	penicillin g ChEBI				yes	10	Unit
[Ref.: #43078]	rifampicin ChEBI				yes	5	µg (disc)
[Ref.: #43078]	streptomycin ChEBI				yes	10	µg (disc)
[Ref.: #43078]	tetracycline ChEBI				yes	30	µg (disc)
[Ref.: #43078]	vancomycin ChEBI				yes	30	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43078]		hydrogen sulfide ChEBI	yes
[Ref.: #43078]		indole ChEBI	no

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #43078]		catalase	+	1.11.1.6
[Ref.: #43078]		cytochrome oxidase	+	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43078]	Sample type/isolated from	subterranean rock salt
[Ref.: #43078]	Geographic location (country and/or sea, region)	Yuanyongjing Salt Mine, Yunnan province, China
[Ref.: #43078]	Country	China
[Ref.: #43078]	Country ISO 3 Code	CHN
[Ref.: #43078]	Continent	Asia
[Ref.: #43078]	Geographic location	25.0000°/101.0000°

[Ref.: #67770]	Sample type/isolated from	Subterranean rock salt of Yuanyongjing Salt Mine
[Ref.: #67770]	Geographic location (country and/or sea, region)	Yunnan
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Condition	#Saline	-
#Environmental	#Terrestrial	#Geologic

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43078]	16S rRNA gene sequences	KJ644242	1471	ENA	1526114 tax ID

[Ref.: #67770]

GC-content

65.1 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #43078]

Culture collection no.

CGMCC 1.15212, JCM 30955

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Halorubrum pallidum sp. nov., an extremely halophilic archaeon isolated from a subterranean rock salt.	Chen S, Liu HC, Zhou J, Xiang H	Int J Syst Evol Microbiol	10.1099/ijsem.0.001129	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43078

Shaoxing Chen, Hong-Can Liu, Jian Zhou and Hua Xiang: Halorubrum pallidum sp. nov., an extremely halophilic archaeon isolated from a subterranean rock salt. IJSEM 66: 2980 - 2986 2016 ( DOI 10.1099/ijsem.0.001129 , PubMed 27150166 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
* These data were automatically processed and therefore are not curated

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