Actinorhabdospora filicis (NBRC 111897, TBRC 5327, DSM 108652)

Name: Actinorhabdospora filicis (NBRC 111897, TBRC 5327, DSM 108652)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 140636

Type strain

Strain Designation K12-0408

Culture col. no. NBRC 111897 TBRC 5327 DSM 108652 K12-0408

NCBI tax ID(s) 1785913

Special Collections DSMZ Literature strains

History <- NBRC; NBRC 111897 <- A. Matsumoto, Kitasato Inst., Tokyo, Japan; K12-0408

Links

Actinorhabdospora filicis K12-0408 is an aerobe, Gram-positive prokaryote that builds an aerial mycelium and was isolated from rhizosphere soils of fern plants.

Gram-positive aerobe 16S sequence

Name and taxonomic classification

SI-ID 404696

TBRC 5327,NBRC 111897,DSM 108652

@ref 20215

Last LPSN update 2025-12-16 10:03:39

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Micromonosporales

Family Micromonosporaceae

Genus Actinorhabdospora

Species Actinorhabdospora filicis

Full scientific name Actinorhabdospora filicis Mingma et al. 2016

Morphology

Cell morphology

@ref	Gram stain	Motility
43070	positive

Multicellular morphology

@ref	Forms multicellular complex	Complex name	Complex color	Medium name	Further description
43070		Aerial mycelium	white	ISP2	forms long chains of cylindrical spores (more than 10 spores per chain) with smooth surfaces
43070		Substrate mycelia	light yellow to pale yellow	ISP2

Pigmentation

@ref	Production	Name
43070		diffusible pigments
43070		melanin pigment

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
43070	Nutrient agar (NA)
43070	PDA
43070	Czapek's agar
43070	ISP4
64815	CZAPEK PEPTONE AGAR (DSMZ Medium 83)	Medium recipe at MediaDive	Name: CZAPEK PEPTONE AGAR (DSMZ Medium 83) Composition: Sucrose 30.0 g/l Agar 20.0 g/l Peptone 5.0 g/l NaNO3 3.0 g/l Yeast extract 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 0.5 g/l KCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Distilled water
64815	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
64815	NUTRIENT AGAR (DSMZ Medium 1)	Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
64815	ISP2 MEDIUM (DSMZ Medium 987)	Medium recipe at MediaDive	Name: ISP 2 MEDIUM (DSMZ Medium 987) Composition: Agar 20.0 g/l Malt extract 10.0 g/l Dextrose 4.0 g/l Yeast extract 4.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
43070	positive	growth	22-30
64815	positive	growth	28	mesophilic

Culture pH

@ref	Ability	Type	PH
43070	positive	growth	6.0-8.0

Physiology and metabolism

Oxygen tolerance

43070

Oxygen toleranceaerobe

Halophily

@ref	Salt	Growth	Tested relation	Concentration
43070	NaCl	positive	growth	1 %(w/v)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43070	48095 ChEBI	(-)-D-fructose	+/-	carbon source
43070		(-)-D-rhamnose	-	carbon source
43070	16708 ChEBI	adenine	-	degradation
43070	36218 ChEBI	beta-lactose	+	carbon source
43070		casein	+	degradation
43070	17057 ChEBI	cellobiose	-	degradation
43070	17057 ChEBI	cellobiose	+	carbon source
43070	17057 ChEBI	cellobiose	+	builds acid from
43070	12936 ChEBI	D-galactose	+	carbon source
43070	16899 ChEBI	D-mannitol	-	carbon source
43070	16024 ChEBI	D-mannose	+	carbon source
43070	16024 ChEBI	D-mannose	+	builds acid from
43070	5291 ChEBI	gelatin	+	degradation
43070	17234 ChEBI	glucose	+	carbon source
43070	17234 ChEBI	glucose	+	builds acid from
43070	17368 ChEBI	hypoxanthine	-	degradation
43070	30849 ChEBI	L-arabinose	-	carbon source
43070	17306 ChEBI	maltose	+	carbon source
43070	17306 ChEBI	maltose	+	builds acid from
43070	28053 ChEBI	melibiose	-	carbon source
43070	17268 ChEBI	myo-inositol	-	carbon source
43070	17632 ChEBI	nitrate	-	reduction
43070	16634 ChEBI	raffinose	-	carbon source
43070	15963 ChEBI	ribitol	-	carbon source
43070	33942 ChEBI	ribose	+	carbon source
43070	27922 ChEBI	sorbose	-	carbon source
43070	28017 ChEBI	starch	-	degradation
43070	17992 ChEBI	sucrose	-	carbon source
43070	53426 ChEBI	tween 80	+	degradation
43070	15318 ChEBI	xanthine	-	degradation
43070	17151 ChEBI	xylitol	-	carbon source
43070	18222 ChEBI	xylose	-	carbon source

Metabolite production

@ref	Chebi-ID	Metabolite	Production
43070	16136 ChEBI	hydrogen sulfide

Enzymes

@ref	Value	Activity	Ec
43070	acid phosphatase	+	3.1.3.2
43070	alkaline phosphatase	-	3.1.3.1
43070	alpha-chymotrypsin	-	3.4.21.1
43070	alpha-fucosidase	+	3.2.1.51
43070	alpha-galactosidase	+	3.2.1.22
43070	alpha-glucosidase	+	3.2.1.20
43070	alpha-mannosidase	-	3.2.1.24
43070	beta-galactosidase	+	3.2.1.23
43070	beta-glucosidase	+	3.2.1.21
43070	beta-glucuronidase	-	3.2.1.31
43070	cystine aminopeptidase	-	3.4.11.3
43070	esterase (C 4)	+
43070	esterase Lipase (C 8)	+
43070	leucine aminopeptidase	+	3.4.1.1
43070	lipase (C 14)	-
43070	N-acetyl-beta-glucosaminidase	-	3.2.1.52
43070	phosphoamidase	+	3.9.1.1
43070	trypsin	+	3.4.21.4
43070	urease	-	3.5.1.5
43070	valine aminopeptidase	+

Fatty acid profile

Metadata FA analysis

@ref

43070

Fatty acid	Percentage
C17:0 anteiso	23.3
anteiso-C17:0 2-OH	17.9
C15:0 iso	9.8
C15:0 anteiso	6.5
C17:0 iso	5.8
10-methyl C17:0	5.75
C17:0	5.4
C16:0 iso	5.1
C16:0 iso 2OH	3.1
10-methyl C18:0	2.19
C17:0 iso 2OH	2

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Rhizosphere

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
43070	rhizosphere soils of fern (pteridophyte) plants	Fussa and Hamura city, located in Tokyo prefecture, Japan	Japan	JPN	Asia
64815	rhizosphere soil of fern	Fussa, Tokyo	Japan	JPN	Asia

Taxonmaps

69479

Global distribution of 16S sequence LC120802 (>99% sequence identity) for Actinorhabdospora filicis subclade from Microbeatlas

Aquatic Samples	23
Soil Samples	1280
Animal Samples	120
Plant Samples	152
Total Samples	1575

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
64815	1	Risk group (German classification)

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
64815	Actinorhabdospora filicis gene for 16S ribosomal RNA, partial sequence	LC120802	1450		1785913

GC content

@ref	GC-content (mol%)	Method
43070	69.6	high performance liquid chromatography (HPLC)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Actinorhabdospora filicis gen. nov., sp. nov., a new member of the family Micromonosporaceae.	Mingma R, Tanaka K, Omura S, Takahashi Y, Matsumoto A	Int J Syst Evol Microbiol	10.1099/ijsem.0.001145	2016

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#43070	Ratchanee Mingma, Kazuki Tanaka, Satoshi Omura, Yoko Takahashi and Atsuko Matsumoto: Actinorhabdospora filicis gen. nov., sp. nov., a new member of the family Micromonosporaceae. IJSEM 66: 3071 - 3077 2016 ( DOI 10.1099/ijsem.0.001145 , PubMed 27169368 )
#64815	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 108652
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Actinorhabdospora filicis (NBRC 111897, TBRC 5327, DSM 108652)

Name and taxonomic classification