Name: Actinorhabdospora filicis K12-0408
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140636

Type strain:

Species: Actinorhabdospora filicis

Strain Designation: K12-0408

Culture col. no.: NBRC 111897, TBRC 5327, DSM 108652

Strain history: <- NBRC; NBRC 111897 <- A. Matsumoto, Kitasato Inst., Tokyo, Japan; K12-0408

NCBI tax ID(s): 1785913 (species)

SI-ID 404696

DSM 108652

Section

Actinorhabdospora filicis K12-0408 is an aerobe, Gram-positive bacterium that builds an aerial mycelium and was isolated from rhizosphere soils of fern plants.

Gram-positive
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micromonosporales*
	Family	*Micromonosporaceae*
	Genus	*Actinorhabdospora*
	Species	**Actinorhabdospora filicis**
	Full Scientific Name (LPSN)	Actinorhabdospora filicis Mingma et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43070]	Gram stain	positive

[Ref.: #43070]	Motility	no

[Ref.: #43070]	Pigment production	no
[Ref.: #43070]	Pigment name	diffusible pigments
[Ref.: #43070]	Pigment production	no
[Ref.: #43070]	Pigment name	Melanin pigments

[Ref.: #43070]	Multicellular complex forming ability	yes
[Ref.: #43070]	Multicellular complex name	Aerial mycelium
[Ref.: #43070]	Multicellular complex color	white
[Ref.: #43070]	Medium Name (multicellularity)	ISP2
[Ref.: #43070]	Multicellular complex further description	forms long chains of cylindrical spores (more than 10 spores per chain) with smooth surfaces

[Ref.: #43070]	Multicellular complex forming ability	yes
[Ref.: #43070]	Multicellular complex name	Substrate mycelia
[Ref.: #43070]	Multicellular complex color	light yellow to pale yellow
[Ref.: #43070]	Medium Name (multicellularity)	ISP2

Information on culture and growth conditions Culture and growth conditions

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Culture medium

Nutrient agar (NA)

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Culture medium growth

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Culture medium

PDA

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Culture medium growth

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Culture medium

Czapek's agar

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Culture medium growth

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Culture medium

ISP4

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Culture medium growth

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Culture medium

CZAPEK PEPTONE AGAR (DSMZ Medium 83)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

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Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

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Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

ISP2 MEDIUM (DSMZ Medium 987)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #43070]			growth	22-30 °C
[Ref.: #64815]			growth	28 °C

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Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #43070]			growth	6.0-8.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43070]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43070]		NaCl		growth	1	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43070]	(-)-D-fructose ChEBI	+/-	carbon source
[Ref.: #43070]	(-)-D-rhamnose	-	carbon source
[Ref.: #43070]	adenine ChEBI	-	degradation
[Ref.: #43070]	beta-lactose ChEBI	+	carbon source
[Ref.: #43070]	casein	+	degradation
[Ref.: #43070]	cellobiose ChEBI	-	degradation
[Ref.: #43070]	cellobiose ChEBI	+	carbon source
[Ref.: #43070]	cellobiose ChEBI	+	builds acid from
[Ref.: #43070]	D-galactose ChEBI	+	carbon source
[Ref.: #43070]	D-mannitol ChEBI	-	carbon source
[Ref.: #43070]	D-mannose ChEBI	+	carbon source
[Ref.: #43070]	D-mannose ChEBI	+	builds acid from
[Ref.: #43070]	gelatin ChEBI	+	degradation
[Ref.: #43070]	glucose ChEBI	+	carbon source
[Ref.: #43070]	glucose ChEBI	+	builds acid from
[Ref.: #43070]	hypoxanthine ChEBI	-	degradation
[Ref.: #43070]	L-arabinose ChEBI	-	carbon source
[Ref.: #43070]	maltose ChEBI	+	carbon source
[Ref.: #43070]	maltose ChEBI	+	builds acid from
[Ref.: #43070]	melibiose ChEBI	-	carbon source
[Ref.: #43070]	myo-inositol ChEBI	-	carbon source
[Ref.: #43070]	nitrate ChEBI	-	reduction
[Ref.: #43070]	raffinose ChEBI	-	carbon source
[Ref.: #43070]	ribitol ChEBI	-	carbon source
[Ref.: #43070]	ribose ChEBI	+	carbon source
[Ref.: #43070]	sorbose ChEBI	-	carbon source
[Ref.: #43070]	starch ChEBI	-	degradation
[Ref.: #43070]	sucrose ChEBI	-	carbon source
[Ref.: #43070]	tween 80 ChEBI	+	degradation
[Ref.: #43070]	xanthine ChEBI	-	degradation
[Ref.: #43070]	xylitol ChEBI	-	carbon source
[Ref.: #43070]	xylose ChEBI	-	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43070]		hydrogen sulfide ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43070]	acid phosphatase	+	3.1.3.2
[Ref.: #43070]	alkaline phosphatase	-	3.1.3.1
[Ref.: #43070]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #43070]	alpha-fucosidase	+	3.2.1.51
[Ref.: #43070]	alpha-galactosidase	+	3.2.1.22
[Ref.: #43070]	alpha-glucosidase	+	3.2.1.20
[Ref.: #43070]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43070]	beta-galactosidase	+	3.2.1.23
[Ref.: #43070]	beta-glucosidase	+	3.2.1.21
[Ref.: #43070]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43070]	cystine aminopeptidase	-	3.4.11.3
[Ref.: #43070]	esterase (C 4)	+
[Ref.: #43070]	esterase Lipase (C 8)	+
[Ref.: #43070]	leucine aminopeptidase	+	3.4.1.1
[Ref.: #43070]	lipase (C 14)	-
[Ref.: #43070]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43070]	phosphoamidase	+	3.9.1.1
[Ref.: #43070]	trypsin	+	3.4.21.4
[Ref.: #43070]	urease	-	3.5.1.5
[Ref.: #43070]	valine aminopeptidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43070]

Fatty acid	Percentage
C17:0 anteiso	23.3
anteiso-C17:0 2-OH	17.9
C15:0 iso	9.8
C15:0 anteiso	6.5
C17:0 iso	5.8
10-methyl C17:0	5.75
C17:0	5.4
C16:0 iso	5.1
C16:0 iso 2OH	3.1
10-methyl C18:0	2.19
C17:0 iso 2OH	2

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43070]	Sample type/isolated from	rhizosphere soils of fern (pteridophyte) plants
[Ref.: #43070]	Geographic location (country and/or sea, region)	Fussa and Hamura city, located in Tokyo prefecture, Japan
[Ref.: #43070]	Country	Japan
[Ref.: #43070]	Country ISO 3 Code	JPN
[Ref.: #43070]	Continent	Asia

[Ref.: #64815]	Sample type/isolated from	rhizosphere soil of fern
[Ref.: #64815]	Geographic location (country and/or sea, region)	Fussa, Tokyo
[Ref.: #64815]	Country	Japan
[Ref.: #64815]	Country ISO 3 Code	JPN
[Ref.: #64815]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Rhizosphere

What are isolation sources categories?

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Global distribution of 16S sequence LC120802 (>99% sequence identity) for Actinorhabdospora filicis subclade from Microbeatlas

Aquatic Samples	23
Soil Samples	1280
Animal Samples	120
Plant Samples	152
Total Samples	1575

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #64815]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64815]	Actinorhabdospora filicis gene for 16S ribosomal RNA, partial sequence	LC120802	1450	ENA	1785913 tax ID

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GC-content

69.6 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #64815]

Culture collection no.

NBRC 111897, TBRC 5327, DSM 108652

[Ref.: #96763]

SI-ID 404696

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Actinorhabdospora filicis gen. nov., sp. nov., a new member of the family Micromonosporaceae.	Mingma R, Tanaka K, Omura S, Takahashi Y, Matsumoto A	Int J Syst Evol Microbiol	10.1099/ijsem.0.001145	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43070

Ratchanee Mingma, Kazuki Tanaka, Satoshi Omura, Yoko Takahashi and Atsuko Matsumoto: Actinorhabdospora filicis gen. nov., sp. nov., a new member of the family Micromonosporaceae. IJSEM 66: 3071 - 3077 2016 ( DOI 10.1099/ijsem.0.001145 , PubMed 27169368 )

#64815

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 108652

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#96763

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID404696.1 )

* These data were automatically processed and therefore are not curated

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