Name: Rhizobium zeae CRZM18R
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 140545

Type strain:

Species: Rhizobium zeae

Strain Designation: CRZM18R

Culture col. no.: LMG 29735, CECT 9169

NCBI tax ID(s): 1905846 (species)

Special Collections

Literature strains

Section

When using BacDive for research please cite our paper

Rhizobium zeae CRZM18R is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from surface disinfected roots of Zea mays plants growing in a soil from Ciudad Rodrigo, Salamanca, Spain.

colony-forming
Gram-negative
rod-shaped
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Rhizobiaceae*
	Genus	*Rhizobium*
	Species	**Rhizobium zeae**
	Full Scientific Name (LPSN)	Rhizobium zeae Celador-Lera et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43333]	Gram stain	negative

[Ref.: #43333]	Cell length	2.0-3.0 µm

[Ref.: #43333]	Cell width	0.6-0.9 µm

[Ref.: #43333]	Cell shape	rod-shaped

[Ref.: #43333]	Colony color	pearl-white
[Ref.: #43333]	Colony shape	circular
[Ref.: #43333]	Cultivation medium used	YMA

Information on culture and growth conditions Culture and growth conditions

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Culture medium

YMA

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Culture medium growth

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Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #43333]	negative	growth	40 °C
[Ref.: #43333]		growth	15-35 °C
[Ref.: #43333]		optimum	28 °C

	Kind of pH		pH
[Ref.: #43333]		optimum	7-7.5
[Ref.: #43333]		growth	6-8
[Ref.: #43333]	no	growth	5

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

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Ability of spore formation

	Salt	Tested relation		Salt conc.
[Ref.: #43333]	NaCl		optimum	1	%(w/v)
[Ref.: #43333]	NaCl		growth	3.5	%(w/v)
[Ref.: #43333]	NaCl	no	growth	4	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43333]	(R)-lactate ChEBI	+	carbon source
[Ref.: #43333]	(S)-lactate ChEBI	+	carbon source
[Ref.: #43333]	2-dehydro-D-gluconate ChEBI	+	carbon source
[Ref.: #43333]	3-hydroxybenzoate ChEBI	+	carbon source
[Ref.: #43333]	3-hydroxybenzoate ChEBI	-	carbon source
[Ref.: #43333]	3-hydroxybutyrate ChEBI	-	carbon source
[Ref.: #43333]	5-dehydro-D-gluconate ChEBI	+	carbon source
[Ref.: #43333]	acetate ChEBI	+	carbon source
[Ref.: #43333]	adipate ChEBI	-	carbon source
[Ref.: #43333]	citrate ChEBI	-	carbon source
[Ref.: #43333]	D-mannose ChEBI	+	builds acid from
[Ref.: #43333]	D-ribose ChEBI	+	builds acid from
[Ref.: #43333]	decanoate ChEBI	-	carbon source
[Ref.: #43333]	esculin ChEBI	+	hydrolysis
[Ref.: #43333]	gluconate ChEBI	+	carbon source
[Ref.: #43333]	glucose ChEBI	-	fermentation
[Ref.: #43333]	glucose ChEBI	+	carbon source
[Ref.: #43333]	glucose ChEBI	+	builds acid from
[Ref.: #43333]	glycogen ChEBI	-	carbon source
[Ref.: #43333]	itaconate ChEBI	-	carbon source
[Ref.: #43333]	L-alanine 4-nitroanilide	+	carbon source
[Ref.: #43333]	L-arabinose ChEBI	+	carbon source
[Ref.: #43333]	L-arabinose ChEBI	+	builds acid from
[Ref.: #43333]	L-fucose ChEBI	+	carbon source
[Ref.: #43333]	L-histidine ChEBI	-	carbon source
[Ref.: #43333]	L-proline ChEBI	+	carbon source
[Ref.: #43333]	L-rhamnose ChEBI	+	carbon source
[Ref.: #43333]	L-rhamnose ChEBI	+	builds acid from
[Ref.: #43333]	L-serine ChEBI	+/-	carbon source
[Ref.: #43333]	L-sorbose ChEBI	+	carbon source
[Ref.: #43333]	malate ChEBI	+	carbon source
[Ref.: #43333]	malonate ChEBI	-	carbon source
[Ref.: #43333]	maltose ChEBI	+	carbon source
[Ref.: #43333]	mannitol ChEBI	+	carbon source
[Ref.: #43333]	mannose ChEBI	+	carbon source
[Ref.: #43333]	mannose ChEBI	+	builds acid from
[Ref.: #43333]	melibiose ChEBI	+	carbon source
[Ref.: #43333]	myo-inositol ChEBI	+	carbon source
[Ref.: #43333]	myo-inositol ChEBI	+	builds acid from
[Ref.: #43333]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #43333]	nitrate ChEBI	+	reduction
[Ref.: #43333]	nitrite ChEBI	+	reduction
[Ref.: #43333]	propionate ChEBI	-	carbon source
[Ref.: #43333]	ribose ChEBI	+	carbon source
[Ref.: #43333]	salicin ChEBI	-	carbon source
[Ref.: #43333]	suberic acid ChEBI	-	carbon source
[Ref.: #43333]	sucrose ChEBI	+	carbon source
[Ref.: #43333]	valerate ChEBI	-	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #43333]	ampicillin ChEBI				yes	2	µg (disc)
[Ref.: #43333]	cefuroxime ChEBI				yes	30	µg (disc)
[Ref.: #43333]	ciprofloxacin ChEBI	yes	5	µg (disc)
[Ref.: #43333]	cloxacillin ChEBI				yes	1	µg (disc)
[Ref.: #43333]	erythromycin ChEBI	yes	2	µg (disc)
[Ref.: #43333]	gentamicin ChEBI	yes	10	µg (disc)
[Ref.: #43333]	neomycin ChEBI	yes	5	µg (disc)
[Ref.: #43333]	penicillin ChEBI				yes	10	Unit (disc)
[Ref.: #43333]	polymyxin b ChEBI				yes	300	Unit (disc)
[Ref.: #43333]	tetracycline ChEBI	yes	30	µg (disc)

	Metabolite	Production
[Ref.: #43333]	hydrogen sulfide ChEBI	no
[Ref.: #43333]	indole ChEBI	no
[Ref.: #43333]	starch ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43333]	acid phosphatase	+/-	3.1.3.2
[Ref.: #43333]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43333]	alpha-arabinosidase	+	3.2.1.55
[Ref.: #43333]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43333]	alpha-galactosidase	+	3.2.1.22
[Ref.: #43333]	alpha-glucosidase	+	3.2.1.20
[Ref.: #43333]	alpha-L-rhamnosidase	-	3.2.1.40
[Ref.: #43333]	alpha-maltosidase	-
[Ref.: #43333]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43333]	alpha-xylosidase	-
[Ref.: #43333]	arginine dihydrolase	-	3.5.3.6
[Ref.: #43333]	beta-cellobiase	+
[Ref.: #43333]	beta-D-fucosidase	+	3.2.1.38
[Ref.: #43333]	beta-galactosidase	+	3.2.1.23
[Ref.: #43333]	beta-glucosidase	+	3.2.1.21
[Ref.: #43333]	beta-L-arabinosidase	-	3.2.1.88
[Ref.: #43333]	beta-maltosidase	+
[Ref.: #43333]	beta-mannosidase	-	3.2.1.25
[Ref.: #43333]	beta-xylosidase	+
[Ref.: #43333]	caseinase	-	3.4.21.50
[Ref.: #43333]	catalase	+	1.11.1.6
[Ref.: #43333]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43333]	galacturonidase	-
[Ref.: #43333]	gelatinase	-
[Ref.: #43333]	lactosidase	+
[Ref.: #43333]	urease	+	3.5.1.5
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

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Fatty acid	Percentage
C18:1ω7c / C18:1ω6c	68.6
C16:0	11.9
C19:0 cyclo ω8c	5.3
C14:0 3OH / C16:1 iso I	4.1
C16:1ω7c / C16:1ω6c	3.3
C16:0 3OH	2.5
C18:1ω7c 11-methyl	1.1
C18:0	1
C18:0 3OH	0.3
C17:0	0.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43333]	Sample type/isolated from	surface disinfected roots of Zea mays (maize) plants growing in a soil from Ciudad Rodrigo, Salamanca, Spain
[Ref.: #43333]	Geographic location (country and/or sea, region)	Ciudad Rodrigo, Salamanca
[Ref.: #43333]	Country	Spain
[Ref.: #43333]	Country ISO 3 Code	ESP
[Ref.: #43333]	Continent	Europe
[Ref.: #43333]	Geographic location	40.0000°/-6.0000°
[Ref.: #43333]	Enrichment culture	yeast mannitol agar (YMA)
[Ref.: #43333]	Enrichment culture composition	0.4 % yeast extract, 1 % mannitol, 0.02 % K2HPO4, 0.02 % KH2PO4, 0.02 % Mg2SO4.7H2O, 0.01 % NaCl and 2 % agar
[Ref.: #43333]	Enrichment culture duration	4
[Ref.: #43333]	Enrichment culture temperature	28 ̊C
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root (Rhizome)
#Host Body-Site	#Plant	#Sterilized plant part

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43333]	Rhizobium zeae strain CRZM18R 16S ribosomal RNA gene, partial sequence	KX932068		GenBank ENA

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GC-content

62.7 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

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Culture collection no.

LMG 29735, CECT 9169

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Rhizobium zeae sp. nov., isolated from maize (Zea mays L.) roots.	Celador-Lera L, Menendez E, Peix A, Igual JM, Velazquez E, Rivas R	Int J Syst Evol Microbiol	10.1099/ijsem.0.001944	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43333

Lorena Celador-Lera, Esther Menéndez, Alvaro Peix, José M. Igual, Encarna Velázquez, Raúl Rivas: Rhizobium zeae sp. nov., isolated from maize (Zea mays L.) roots. IJSEM 67: 2306 - 2311 2017 ( DOI 10.1099/ijsem.0.001944 , PubMed 28699873 )

* These data were automatically processed and therefore are not curated

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