Name: Mesorhizobium helmanticense CSLC115
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140544

Type strain:

Species: Mesorhizobium helmanticense

Strain Designation: CSLC115

Culture col. no.: CECT 9168, LMG 29734

NCBI tax ID(s): 1776423 (species)

Section

Mesorhizobium helmanticense CSLC115 is an aerobe, chemoorganotroph, mesophilic bacterium that forms circular colonies and was isolated from effective nodules ol Lotus corniculatus plants growing in a soil, Carbajosa de la Sagrada, Salamanca, Spain.

colony-forming
Gram-negative
motile
rod-shaped
aerobe
chemoorganotroph
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Phyllobacteriaceae*
	Genus	*Mesorhizobium*
	Species	**Mesorhizobium helmanticense**
	Full Scientific Name (LPSN)	Mesorhizobium helmanticense Marcos-García et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43332]	Gram stain	negative

[Ref.: #43332]	Cell length	1.5-3.0 µm

[Ref.: #43332]	Cell width	0.3-0.5 µm

[Ref.: #43332]	Cell shape	rod-shaped

[Ref.: #43332]	Motility	yes

[Ref.: #43332]	Colony size	1-2 mm
[Ref.: #43332]	Colony color	white
[Ref.: #43332]	Colony shape	circular
[Ref.: #43332]	Incubation period	4-5 days
[Ref.: #43332]	Cultivation medium used	YMA

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43332]

Culture medium

YMA

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Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #43332]			optimum	28 °C
[Ref.: #43332]			growth	5-35 °C

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Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #43332]			optimum	7
[Ref.: #43332]			growth	5-9

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

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Nutrition type

chemoorganotroph

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Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43332]		NaCl		growth	1	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43332]	(R)-lactate ChEBI	+/-	carbon source
[Ref.: #43332]	(S)-lactate ChEBI	+/-	carbon source
[Ref.: #43332]	2-dehydro-D-gluconate ChEBI	-	carbon source
[Ref.: #43332]	3-hydroxybenzoate ChEBI	-	carbon source
[Ref.: #43332]	3-hydroxybutyrate ChEBI	-	carbon source
[Ref.: #43332]	4-hydroxybenzoate ChEBI	-	carbon source
[Ref.: #43332]	5-dehydro-D-gluconate ChEBI	+/-	carbon source
[Ref.: #43332]	acetate ChEBI	+/-	carbon source
[Ref.: #43332]	adipate ChEBI	-	carbon source
[Ref.: #43332]	citrate ChEBI	-	carbon source
[Ref.: #43332]	D-mannose ChEBI	+	carbon source
[Ref.: #43332]	D-ribose ChEBI	+	carbon source
[Ref.: #43332]	D-sorbitol ChEBI	+	carbon source
[Ref.: #43332]	decanoate ChEBI	-	carbon source
[Ref.: #43332]	esculin ChEBI	+	hydrolysis
[Ref.: #43332]	gluconate ChEBI	-	carbon source
[Ref.: #43332]	glucose ChEBI	+	carbon source
[Ref.: #43332]	glycogen ChEBI	+/-	carbon source
[Ref.: #43332]	itaconate ChEBI	-	carbon source
[Ref.: #43332]	L-arabinose ChEBI	+	carbon source
[Ref.: #43332]	L-fucose ChEBI	+	carbon source
[Ref.: #43332]	L-histidine ChEBI	+	carbon source
[Ref.: #43332]	L-proline ChEBI	+	carbon source
[Ref.: #43332]	L-rhamnose ChEBI	+	carbon source
[Ref.: #43332]	L-serine ChEBI	-	carbon source
[Ref.: #43332]	malate ChEBI	+	carbon source
[Ref.: #43332]	maltose ChEBI	+	carbon source
[Ref.: #43332]	mannitol ChEBI	+	builds acid from
[Ref.: #43332]	mannitol ChEBI	+	carbon source
[Ref.: #43332]	melibiose ChEBI	+	carbon source
[Ref.: #43332]	myo-inositol ChEBI	+	carbon source
[Ref.: #43332]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #43332]	nitrate ChEBI	-	reduction
[Ref.: #43332]	phenylacetate ChEBI	-	carbon source
[Ref.: #43332]	propionate ChEBI	-	carbon source
[Ref.: #43332]	salicin ChEBI	+	carbon source
[Ref.: #43332]	sodium malonate ChEBI	-	carbon source
[Ref.: #43332]	suberic acid ChEBI	-	carbon source
[Ref.: #43332]	sucrose ChEBI	+	carbon source
[Ref.: #43332]	valerate ChEBI	-	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #43332]	ampicillin ChEBI				yes	2	µg (disc)
[Ref.: #43332]	cefuroxime ChEBI	yes	30	µg (disc)
[Ref.: #43332]	ciprofloxacin ChEBI	yes	5	µg (disc)
[Ref.: #43332]	cloxacillin ChEBI				yes	1	µg (disc)
[Ref.: #43332]	erythromycin ChEBI				yes	2	µg (disc)
[Ref.: #43332]	gentamicin ChEBI				yes	10	µg (disc)
[Ref.: #43332]	neomycin ChEBI	yes	5	µg (disc)
[Ref.: #43332]	penicillin ChEBI	yes	10	Unit (disc)
[Ref.: #43332]	polymyxin b ChEBI				yes	300	Unit (disc)
[Ref.: #43332]	tetracycline ChEBI	yes	30	µg (disc)

	Metabolite production	Metabolite	Production
[Ref.: #43332]		hydrogen sulfide ChEBI	no
[Ref.: #43332]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43332]	acid phosphatase	-	3.1.3.2
[Ref.: #43332]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43332]	alpha-arabinosidase	-	3.2.1.55
[Ref.: #43332]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43332]	alpha-galactosidase	-	3.2.1.22
[Ref.: #43332]	alpha-glucosidase	+	3.2.1.20
[Ref.: #43332]	alpha-L-rhamnosidase	-	3.2.1.40
[Ref.: #43332]	alpha-maltosidase	+
[Ref.: #43332]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43332]	alpha-xylosidase	-
[Ref.: #43332]	arginine dihydrolase	-	3.5.3.6
[Ref.: #43332]	beta-cellobiase	-
[Ref.: #43332]	beta-D-fucosidase	-	3.2.1.38
[Ref.: #43332]	beta-galactosidase	+	3.2.1.23
[Ref.: #43332]	beta-glucosidase	+	3.2.1.21
[Ref.: #43332]	beta-L-arabinosidase	-	3.2.1.88
[Ref.: #43332]	beta-maltosidase	-
[Ref.: #43332]	beta-mannosidase	-	3.2.1.25
[Ref.: #43332]	beta-xylosidase	-
[Ref.: #43332]	catalase	+	1.11.1.6
[Ref.: #43332]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43332]	galacturonidase	-
[Ref.: #43332]	gelatinase	-
[Ref.: #43332]	glucuronidase	-
[Ref.: #43332]	lactosidase	-
[Ref.: #43332]	urease	+	3.5.1.5
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43332]

Fatty acid	Percentage
C18:1ω7c / C18:1ω6c	42.8
C18:1ω7c 11-methyl	17.8
C16:0	12.9
C19:0 cyclo ω8c	7.1
C17:0 iso	5.9
C20:2ω6,9c	3.2
C18:0	2.2
C16:1ω7c / C16:1ω6c	1.9
C13:0 iso 3OH	1.7
C19:0 10-methyl	1.6
C12:0 3OH	0.6
C12:0 2OH	0.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43332]	Sample type/isolated from	effective nodules ol Lotus corniculatus plants growing in a soil, Carbajosa de la Sagrada, Salamanca, Spain
[Ref.: #43332]	Geographic location (country and/or sea, region)	Carbajosa de la Sagrada, Salamanca
[Ref.: #43332]	Country	Spain
[Ref.: #43332]	Country ISO 3 Code	ESP
[Ref.: #43332]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root nodule

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KT899885 (>99% sequence identity) for Mesorhizobium from Microbeatlas

Aquatic Samples	507
Soil Samples	2635
Animal Samples	282
Plant Samples	1035
Total Samples	4459

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43332]	16S rRNA gene sequence	KT899885		GenBank

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GC-content

62.7 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #43332]

Culture collection no.

CECT 9168, LMG 29734

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Mesorhizobium helmanticense sp. nov., isolated from Lotus corniculatus nodules.	Marcos-Garcia M, Menendez E, Ramirez-Bahena MH, Mateos PF, Peix A, Velazquez E, Rivas R	Int J Syst Evol Microbiol	10.1099/ijsem.0.001942	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43332

Marta Marcos-García, Esther Menéndez, Marta Helena Ramírez-Bahena, Pedro F. Mateos, Álvaro Peix, Encarna Velazquez, Raúl Rivas: Mesorhizobium helmanticense sp. nov., isolated from Lotus corniculatus nodules. IJSEM 67: 2301 - 2305 2017 ( DOI 10.1099/ijsem.0.001942 , PubMed 28693668 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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