Name: Roseateles oligotrophus CHU3
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140540

Type strain:

Species: Roseateles oligotrophus

Strain Designation: CHU3

Culture col. no.: KCTC 42519, CICC 24092, DSM 109232

Strain history: <- Kim, Song-Gun, KRIBB

NCBI tax ID(s): 1769250 (species)

SI-ID 406516

DSM 109232

Section

Roseateles oligotrophus CHU3 is an aerobe, heterotroph, mesophilic bacterium that forms circular colonies and was isolated from Fresh water collected from the Daecheong Reservoir.

colony-forming
Gram-negative
motile
rod-shaped
aerobe
heterotroph
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Comamonadaceae*
	Genus	*Roseateles*
	Species	**Roseateles oligotrophus**
	Full Scientific Name (LPSN)	Roseateles oligotrophus (Pheng et al. 2017) Liu et al. 2023

Synonym

Paucibacter oligotrophus

Information on morphological and physiological properties Morphology

[Ref.: #43327]	Gram stain	negative

[Ref.: #43327]	Cell length	1.5-5 µm

[Ref.: #43327]	Cell width	0.5-1 µm

[Ref.: #43327]	Cell shape	rod-shaped

[Ref.: #43327]	Motility	yes

[Ref.: #43327]	Flagellum arrangement	polar

[Ref.: #43327]	Colony size	1 mm
[Ref.: #43327]	Colony color	off-white
[Ref.: #43327]	Colony shape	circular
[Ref.: #43327]	Cultivation medium used	R2A

Multimedia content

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

Reasoner's 2A agar (R2A)

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Culture medium growth

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Culture medium

R2A MEDIUM (DSMZ Medium 830)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

LB (Luria-Bertani) MEDIUM

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Culture medium growth

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Culture medium

Nutrient agar (NA)

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Culture medium growth

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Culture medium

Trypticase Soy Agar (TSA)

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Culture medium growth

	Kind of temperature		Temperature
[Ref.: #43327]		maximum	30 °C
[Ref.: #43327]	no	growth	10 °C
[Ref.: #43327]	no	growth	4 °C
[Ref.: #43327]		optimum	25 °C
[Ref.: #43327]		growth	15-30 °C
[Ref.: #64860]		growth	25 °C
[Ref.: #67771]		growth	25 °C

[Ref.: #43327]	Temperature range	mesophilic
[Ref.: #43327]	Temperature range	psychrophilic
[Ref.: #43327]	Temperature range	mesophilic
[Ref.: #64860]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43327]			optimum	7
[Ref.: #43327]			growth	6-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43327]	Oxygen tolerance	aerobe
[Ref.: #43327]	Oxygen tolerance	anaerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

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Nutrition type

heterotroph

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Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43327]		NaCl		growth	0-1	%(w/v)
[Ref.: #43327]		NaCl		optimum	0	%(w/v)

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Observation

quinone Q-8

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43327]	dna ChEBI	+	hydrolysis
[Ref.: #43327]	esculin ChEBI	+	hydrolysis
[Ref.: #43327]	gelatin ChEBI	-	hydrolysis
[Ref.: #43327]	L-alanine ChEBI	+	carbon source
[Ref.: #43327]	L-arabinose ChEBI	+	carbon source
[Ref.: #43327]	L-aspartate ChEBI	+	carbon source
[Ref.: #43327]	nitrate ChEBI	+	reduction
[Ref.: #43327]	sodium pyruvate ChEBI	-	assimilation
[Ref.: #43327]	starch ChEBI	+	hydrolysis
[Ref.: #43327]	urea ChEBI	-	hydrolysis

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #43327]		ampicillin ChEBI	yes
[Ref.: #43327]		lincomycin ChEBI		yes

	Metabolite production	Metabolite	Production
[Ref.: #43327]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43327]	acid phosphatase	+	3.1.3.2
[Ref.: #43327]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43327]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #43327]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43327]	alpha-galactosidase	-	3.2.1.22
[Ref.: #43327]	alpha-glucosidase	-	3.2.1.20
[Ref.: #43327]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43327]	beta-galactosidase	-	3.2.1.23
[Ref.: #43327]	beta-galactosidase	+	3.2.1.23
[Ref.: #43327]	beta-glucosidase	+	3.2.1.21
[Ref.: #43327]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43327]	catalase	+	1.11.1.6
[Ref.: #43327]	cystine arylamidase	+	3.4.11.3
[Ref.: #43327]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43327]	esterase (C 4)	+/-
[Ref.: #43327]	esterase Lipase (C 8)	+/-
[Ref.: #43327]	leucine arylamidase	+	3.4.11.1
[Ref.: #43327]	lipase (C 14)	-
[Ref.: #43327]	N-acetyl-beta-glucosaminidase	+/-	3.2.1.52
[Ref.: #43327]	naphthol-AS-BI-phosphohydrolase	+/-
[Ref.: #43327]	trypsin	+	3.4.21.4
[Ref.: #43327]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

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Fatty acid	Percentage
C16:1ω7c / C16:1ω6c	62
C16:0	17.1
C12:0	4.8
C15:1ω6c	4.5
C10:0 3OH	2.5
C17:0	2.3
C19:1ω6c / unknown 18.846	1.9
C18:1ω7c / C18:1ω6c	1.3
C14:0	1
C10:0	0.8
C16:1ω7c alcohol	0.6

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43327]	Sample type/isolated from	Fresh water collected from the Daecheong Reservoir
[Ref.: #43327]	Geographic location (country and/or sea, region)	Daecheong Reservoir
[Ref.: #43327]	Country	Republic of Korea
[Ref.: #43327]	Country ISO 3 Code	KOR
[Ref.: #43327]	Continent	Asia
[Ref.: #43327]	Geographic location	36.0000°/127.0000°
[Ref.: #43327]	Enrichment culture	R2A agar

[Ref.: #64860]	Sample type/isolated from	fresh water
[Ref.: #64860]	Geographic location (country and/or sea, region)	Chudong, Daechong reservoir, Daecheong Dam (36° 28' 39'' N, 127° 28' 51'' E)
[Ref.: #64860]	Country	Republic of Korea
[Ref.: #64860]	Country ISO 3 Code	KOR
[Ref.: #64860]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From fresh water in Daecheong Dam
[Ref.: #67771]	Geographic location (country and/or sea, region)	Chudong
[Ref.: #67771]	Country	Republic of Korea
[Ref.: #67771]	Country ISO 3 Code	KOR
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Built environment	#Container (Reservoir)
#Environmental	#Aquatic	#Freshwater

What are isolation sources categories?

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Global distribution of 16S sequence KT333459 (>99% sequence identity) for Paucibacter oligotrophus from Microbeatlas

Aquatic Samples	225
Soil Samples	18
Animal Samples	45
Plant Samples	3
Total Samples	291

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

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Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64860]	Paucibacter oligotrophus strain KCTC 42519 16S ribosomal RNA gene, partial sequence	KT333459	1448	ENA	1769250 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Paucibacter oligotrophus CHU3	GCA_025717515	contig	GenBank	1769250 tax ID	*

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GC-content

62.5 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Paucibacter oligotrophus CHU3 NCBI: GCA_025717515.1
[Ref.: #69480]	motile	yes	85.451	yes
[Ref.: #69480]	flagellated	yes	78.98	no
[Ref.: #69480]	gram-positive	no	98.442	yes
[Ref.: #69480]	anaerobic	no	97.975	no
[Ref.: #69480]	aerobic	yes	90.857	no
[Ref.: #69480]	halophile	no	96.52	yes
[Ref.: #69480]	spore-forming	no	94.666	no
[Ref.: #69480]	glucose-ferment	no	82.333	no
[Ref.: #69480]	thermophile	no	98.004	yes
[Ref.: #69480]	glucose-util	yes	74.698	no

Availability in culture collections External links

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Culture collection no.

KCTC 42519, CICC 24092, DSM 109232

[Ref.: #96739]

SI-ID 406516

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Paucibacter oligotrophus sp. nov., isolated from fresh water, and emended description of the genus Paucibacter.	Pheng S, Lee JJ, Eom MK, Lee KH, Kim SG	Int J Syst Evol Microbiol	10.1099/ijsem.0.001931	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43327

Sophea Pheng, Jay Jung Lee, Mi Kyung Eom, Kang Hyun Lee, Song-Gun Kim: Paucibacter oligotrophus sp. nov., isolated from fresh water, and emended description of the genus Paucibacter. IJSEM 67: 2231 - 2235 2017 ( DOI 10.1099/ijsem.0.001931 , PubMed 28671521 )

#64860

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 109232

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#96739

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID406516.1 )

* These data were automatically processed and therefore are not curated

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Paucibacter oligotrophus CHU3

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