Name: Massilia pinisoli T33
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140511

Type strain:

Species: Massilia pinisoli

Strain Designation: T33

Culture col. no.: KACC 18748, KEMB 9005-368, JCM 31316

Strain history: J. Kim; Kyonggi Univ., South Korea; T33.

NCBI tax ID(s): 1772194 (species)

Section

Massilia pinisoli T33 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from soil sample.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Oxalobacteraceae*
	Genus	*Massilia*
	Species	**Massilia pinisoli**
	Full Scientific Name (LPSN)	Massilia pinisoli Altankhuu and Kim 2016

Synonym

Telluria pinisoli

Information on morphological and physiological properties Morphology

[Ref.: #43166]	Gram stain	negative

[Ref.: #43166]	Cell length	2.0-2.5 µm

[Ref.: #43166]	Cell width	0.9-1.5 µm

[Ref.: #43166]	Cell shape	rod-shaped

[Ref.: #43166]	Motility	yes

[Ref.: #43166]	Flagellum arrangement	monotrichous, polar

[Ref.: #43166]	Colony size	1.0-2.0 mm
[Ref.: #43166]	Colony color	white
[Ref.: #43166]	Incubation period	2 days
[Ref.: #43166]	Cultivation medium used	R2A agar

Information on culture and growth conditions Culture and growth conditions

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Culture medium

Reasoner's 2A agar (R2A)

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Culture medium growth

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Culture medium

LB (Luria-Bertani) MEDIUM

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Culture medium growth

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Culture medium

tryptic soy agar (TSA)

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Culture medium growth

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Culture medium

MacConkey agar

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Culture medium growth

		Temperature
[Ref.: #43166]	optimum	32-37 °C
[Ref.: #43166]	growth	10-37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #43166]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43166]			optimum	6.0-7.0
[Ref.: #43166]			growth	5.0-9.0

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

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Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43166]		NaCl		growth	0-1	%(w/v)
[Ref.: #43166]		NaCl		optimum	0	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43166]	3-hydroxybenzoate ChEBI	-	assimilation
[Ref.: #43166]	4-nitrophenyl beta-D-galactopyranoside ChEBI	+	assimilation
[Ref.: #43166]	casein	-	hydrolysis
[Ref.: #43166]	D-glucose ChEBI	+	assimilation
[Ref.: #43166]	dna ChEBI	-	hydrolysis
[Ref.: #43166]	esculin ChEBI	+	hydrolysis
[Ref.: #43166]	gelatin ChEBI	-	hydrolysis
[Ref.: #43166]	glycogen ChEBI	+	assimilation
[Ref.: #43166]	L-proline ChEBI	-	assimilation
[Ref.: #43166]	L-rhamnose ChEBI	-	assimilation
[Ref.: #43166]	lactate ChEBI	-	assimilation
[Ref.: #43166]	nitrate ChEBI	+	reduction
[Ref.: #43166]	sodium acetate ChEBI	-	assimilation
[Ref.: #43166]	sodium citrate ChEBI	-	assimilation
[Ref.: #43166]	starch ChEBI	-	hydrolysis
[Ref.: #43166]	suberic acid ChEBI	-	assimilation
[Ref.: #43166]	sucrose ChEBI	+	assimilation
[Ref.: #43166]	tween 80 ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #43166]	alpha-galactosidase	+	3.2.1.22
[Ref.: #43166]	alpha-glucosidase	+	3.2.1.20
[Ref.: #43166]	beta-galactosidase	+	3.2.1.23
[Ref.: #43166]	beta-glucosidase	+	3.2.1.21
[Ref.: #43166]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43166]	catalase	+	1.11.1.6
[Ref.: #43166]	cystine arylamidase	+	3.4.11.3
[Ref.: #43166]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43166]	esterase Lipase (C 8)	+
[Ref.: #43166]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43166]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43166]	urease	-	3.5.1.5
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43166]

Fatty acid	Percentage
C16:1ω6c / C16:1ω7c	50.6
C16:0	23.1
C18:1ω6c / C18:1ω7c	11.8
C14:0	5.1
C12:0	2.8
C10:0 3OH	2.4
C17:0 cylco	2.3

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43166]	Sample type/isolated from	soil sample
[Ref.: #43166]	Geographic location (country and/or sea, region)	Kyonggi University, Suwon
[Ref.: #43166]	Country	Republic of Korea
[Ref.: #43166]	Country ISO 3 Code	KOR
[Ref.: #43166]	Continent	Asia
[Ref.: #43166]	Enrichment culture	R2A medium
[Ref.: #43166]	Isolation/enrichment procedure	modified culture method using transwell plates

[Ref.: #67770]	Sample type/isolated from	Forest soil at Kyonggi Univ.
[Ref.: #67770]	Geographic location (country and/or sea, region)	Suwon
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43166]	Massilia pinisoli strain T33 16S ribosomal RNA gene, partial sequence	KU296190	1472	GenBank	1772194 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Massilia pinisoli JCM 31316	GCA_024753285	contig	GenBank	1772194 tax ID	*

[Ref.: #43166]	GC-content	69.4 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	69.4 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Massilia pinisoli JCM 31316 NCBI: GCA_024753285.1
[Ref.: #69480]	motile	yes	89.129	yes
[Ref.: #69480]	flagellated	yes	82.365	yes
[Ref.: #69480]	gram-positive	no	98.505	yes
[Ref.: #69480]	anaerobic	no	99.548	no
[Ref.: #69480]	aerobic	yes	93.615	yes
[Ref.: #69480]	halophile	no	95.464	no
[Ref.: #69480]	spore-forming	no	93.936	yes
[Ref.: #69480]	glucose-ferment	no	89.165	no
[Ref.: #69480]	thermophile	no	99.41	yes
[Ref.: #69480]	glucose-util	yes	93.157	no

Availability in culture collections External links

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Culture collection no.

KACC 18748, KEMB 9005-368, JCM 31316

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Massiliapinisoli sp. nov., isolated from forest soil.	Altankhuu K, Kim J	Int J Syst Evol Microbiol	10.1099/ijsem.0.001249	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43166

Khulan Altankhuu, Jaisoo Kim: Massilia pinisoli sp. nov., isolated from forest soil. IJSEM 66: 3669 - 3674 2016 ( DOI 10.1099/ijsem.0.001249 , PubMed 27334790 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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Massilia pinisoli JCM 31316

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