Name: Longibacter salinarum WDS2C18
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140500

Type strain:

Species: Longibacter salinarum

Strain Designation: WDS2C18

Culture col. no.: KCTC 52045, MCCC 1H00132

NCBI tax ID(s): 1850348 (species)

Section

Longibacter salinarum WDS2C18 is a facultative anaerobe, halophilic, heterotroph bacterium that forms circular colonies and was isolated from sediment sample from a marine solar saltern.

colony-forming
Gram-negative
rod-shaped
facultative anaerobe
halophilic
heterotroph
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Rhodothermota*
	Class	*Rhodothermia*
	Order	*Rhodothermales*
	Family	*Salinibacteraceae*
	Genus	*Longibacter*
	Species	**Longibacter salinarum**
	Full Scientific Name (LPSN)	Longibacter salinarum Xia et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43119]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.999

[Ref.: #43119]	Cell length	6.0-12.0 µm

[Ref.: #43119]	Cell width	0.3-0.4 µm

[Ref.: #43119]	Cell shape	rod-shaped

[Ref.: #43119]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %92.175

[Ref.: #43119]	Colony color	red
[Ref.: #43119]	Colony shape	circular
[Ref.: #43119]	Cultivation medium used	modified marine agar 2216 (MA; BD Difco)

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43119]

Culture medium

modified marine agar 2216 (MA; BD Difco)

[Ref.: #43119]

Culture medium growth

[Ref.: #43119]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #43119]			optimum	40 °C
[Ref.: #43119]			growth	20-50 °C

[Ref.: #43119]

Temperature range

thermophilic

	pH	Kind of pH		pH
[Ref.: #43119]			optimum	7.5-8.0
[Ref.: #43119]			growth	6.5-8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43119]

Oxygen tolerance

facultative anaerobe

[Ref.: #43119]

Nutrition type

heterotroph

[Ref.: #69481]	Ability of spore formation	no Confidence in %98
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.958

[Ref.: #43119]

Halophily / tolerance level

halophilic

	Salt		Salt conc.
[Ref.: #43119]	NaCl	growth	2-20	%(w/v)
[Ref.: #43119]	NaCl	optimum	8-12	%(w/v)
[Ref.: #43119]	MgCl2x6H2O	growth	0-12	%(w/v)
[Ref.: #43119]	MgCl2x6H2O	optimum	0-4	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43119]	agar ChEBI	-	hydrolysis
[Ref.: #43119]	carboxymethylcellulose ChEBI	-	hydrolysis
[Ref.: #43119]	cellobiose ChEBI	-	growth
[Ref.: #43119]	cellobiose ChEBI	+	builds acid from
[Ref.: #43119]	D-arabinose ChEBI	-	growth
[Ref.: #43119]	D-fructose ChEBI	-	growth
[Ref.: #43119]	D-fructose ChEBI	+	builds acid from
[Ref.: #43119]	D-galactose ChEBI	-	growth
[Ref.: #43119]	D-mannitol ChEBI	+	growth
[Ref.: #43119]	D-mannose ChEBI	-	growth
[Ref.: #43119]	D-mannose ChEBI	-	builds acid from
[Ref.: #43119]	D-ribose ChEBI	-	growth
[Ref.: #43119]	D-ribose ChEBI	+	builds acid from
[Ref.: #43119]	D-tagatose ChEBI	+	builds acid from
[Ref.: #43119]	D-xylose ChEBI	-	growth
[Ref.: #43119]	esculin ChEBI	+	builds acid from
[Ref.: #43119]	glucose ChEBI	-	growth
[Ref.: #43119]	glycerol ChEBI	+	growth
[Ref.: #43119]	glycerol ChEBI	-	builds acid from
[Ref.: #43119]	L-rhamnose ChEBI	-	growth
[Ref.: #43119]	L-sorbose ChEBI	+	builds acid from
[Ref.: #43119]	lactose ChEBI	-	growth
[Ref.: #43119]	maltose ChEBI	-	growth
[Ref.: #43119]	nitrate ChEBI	-	reduction
[Ref.: #43119]	potassium 2-dehydro-D-gluconate	-	builds acid from
[Ref.: #43119]	potassium 5-dehydro-D-gluconate	+	builds acid from
[Ref.: #43119]	sodium alginate ChEBI	-	hydrolysis
[Ref.: #43119]	starch ChEBI	+	hydrolysis
[Ref.: #43119]	starch ChEBI	+	growth
[Ref.: #43119]	sucrose ChEBI	+	growth
[Ref.: #43119]	tween 20 ChEBI	-	hydrolysis
[Ref.: #43119]	tween 40 ChEBI	+	hydrolysis
[Ref.: #43119]	tween 80 ChEBI	+	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #43119]	acid phosphatase	+	3.1.3.2
[Ref.: #43119]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43119]	catalase	+	1.11.1.6
[Ref.: #43119]	chymotrypsin	+	3.4.4.5
[Ref.: #43119]	cystine arylamidase	-	3.4.11.3
[Ref.: #43119]	cytochrome oxidase	-	1.9.3.1
[Ref.: #43119]	esterase (C 4)	+
[Ref.: #43119]	esterase Lipase (C 8)	+
[Ref.: #43119]	gelatinase	+
[Ref.: #43119]	leucine arylamidase	+	3.4.11.1
[Ref.: #43119]	trypsin	-	3.4.21.4
[Ref.: #43119]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43119]

Fatty acid	Percentage
C16:1ω7c / C15:0 iso 2OH	22.5
C17:1 iso ω9c	21.6
C15:0 iso	17.9
C17:0 iso	13.4
C17:0 anteiso	6.5
C17:0 iso 3OH	2.9
C16:0 iso	2.6
C18:1ω7c	2.4
C16:0	2.2
C16:1ω5c	1.1

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	cardiolipin biosynthesis	100	7 of 7
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	denitrification	100	2 of 2
[Ref.: #66794]	glycogen metabolism	100	5 of 5
[Ref.: #66794]	sulfopterin metabolism	100	4 of 4
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	vitamin K metabolism	100	5 of 5
[Ref.: #66794]	cis-vaccenate biosynthesis	100	2 of 2
[Ref.: #66794]	methylglyoxal degradation	100	5 of 5
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	suberin monomers biosynthesis	100	2 of 2
[Ref.: #66794]	C4 and CAM-carbon fixation	100	8 of 8
[Ref.: #66794]	starch degradation	100	10 of 10
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	palmitate biosynthesis	90.91	20 of 22
[Ref.: #66794]	threonine metabolism	90	9 of 10
[Ref.: #66794]	d-mannose degradation	88.89	8 of 9
[Ref.: #66794]	serine metabolism	88.89	8 of 9
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	aspartate and asparagine metabolism	88.89	8 of 9
[Ref.: #66794]	CO2 fixation in Crenarchaeota	88.89	8 of 9
[Ref.: #66794]	gluconeogenesis	87.5	7 of 8
[Ref.: #66794]	photosynthesis	85.71	12 of 14
[Ref.: #66794]	tetrahydrofolate metabolism	85.71	12 of 14
[Ref.: #66794]	phenylalanine metabolism	84.62	11 of 13
[Ref.: #66794]	NAD metabolism	83.33	15 of 18
[Ref.: #66794]	pentose phosphate pathway	81.82	9 of 11
[Ref.: #66794]	vitamin B6 metabolism	81.82	9 of 11
[Ref.: #66794]	glycine betaine biosynthesis	80	4 of 5
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	cellulose degradation	80	4 of 5
[Ref.: #66794]	propionate fermentation	80	8 of 10
[Ref.: #66794]	glutamate and glutamine metabolism	78.57	22 of 28
[Ref.: #66794]	purine metabolism	75.53	71 of 94
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	tyrosine metabolism	71.43	10 of 14
[Ref.: #66794]	pyrimidine metabolism	71.11	32 of 45
[Ref.: #66794]	glycolysis	70.59	12 of 17
[Ref.: #66794]	vitamin B1 metabolism	69.23	9 of 13
[Ref.: #66794]	L-lactaldehyde degradation	66.67	2 of 3
[Ref.: #66794]	flavin biosynthesis	66.67	10 of 15
[Ref.: #66794]	formaldehyde oxidation	66.67	2 of 3
[Ref.: #66794]	acetyl CoA biosynthesis	66.67	2 of 3
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	tryptophan metabolism	65.79	25 of 38
[Ref.: #66794]	isoprenoid biosynthesis	65.38	17 of 26
[Ref.: #66794]	lysine metabolism	64.29	27 of 42
[Ref.: #66794]	citric acid cycle	64.29	9 of 14
[Ref.: #66794]	metabolism of disaccharids	63.64	7 of 11
[Ref.: #66794]	ketogluconate metabolism	62.5	5 of 8
[Ref.: #66794]	dTDPLrhamnose biosynthesis	62.5	5 of 8
[Ref.: #66794]	histidine metabolism	62.07	18 of 29
[Ref.: #66794]	alanine metabolism	62.07	18 of 29
[Ref.: #66794]	methionine metabolism	61.54	16 of 26
[Ref.: #66794]	non-pathway related	60.53	23 of 38
[Ref.: #66794]	lipoate biosynthesis	60	3 of 5
[Ref.: #66794]	metabolism of amino sugars and derivatives	60	3 of 5
[Ref.: #66794]	arginine metabolism	58.33	14 of 24
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	58.33	7 of 12
[Ref.: #66794]	lipid metabolism	58.06	18 of 31
[Ref.: #66794]	propanol degradation	57.14	4 of 7
[Ref.: #66794]	ubiquinone biosynthesis	57.14	4 of 7
[Ref.: #66794]	heme metabolism	57.14	8 of 14
[Ref.: #66794]	cysteine metabolism	55.56	10 of 18
[Ref.: #66794]	proline metabolism	54.55	6 of 11
[Ref.: #66794]	leucine metabolism	53.85	7 of 13
[Ref.: #66794]	phenylpropanoid biosynthesis	53.85	7 of 13
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	53.85	7 of 13
[Ref.: #66794]	pantothenate biosynthesis	50	3 of 6
[Ref.: #66794]	cyclohexanol degradation	50	2 of 4
[Ref.: #66794]	toluene degradation	50	2 of 4
[Ref.: #66794]	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	glycolate and glyoxylate degradation	50	3 of 6
[Ref.: #66794]	kanosamine biosynthesis II	50	1 of 2
[Ref.: #66794]	CMP-KDO biosynthesis	50	2 of 4
[Ref.: #66794]	isoleucine metabolism	50	4 of 8
[Ref.: #66794]	glutathione metabolism	50	7 of 14
[Ref.: #66794]	Entner Doudoroff pathway	50	5 of 10
[Ref.: #66794]	phenylmercury acetate degradation	50	1 of 2
[Ref.: #66794]	coenzyme M biosynthesis	50	5 of 10
[Ref.: #66794]	urea cycle	46.15	6 of 13
[Ref.: #66794]	oxidative phosphorylation	46.15	42 of 91
[Ref.: #66794]	carotenoid biosynthesis	45.45	10 of 22
[Ref.: #66794]	nitrate assimilation	44.44	4 of 9
[Ref.: #66794]	lipid A biosynthesis	44.44	4 of 9
[Ref.: #66794]	benzoyl-CoA degradation	42.86	3 of 7
[Ref.: #66794]	reductive acetyl coenzyme A pathway	42.86	3 of 7
[Ref.: #66794]	gallate degradation	40	2 of 5
[Ref.: #66794]	phenylacetate degradation (aerobic)	40	2 of 5
[Ref.: #66794]	glycine metabolism	40	4 of 10
[Ref.: #66794]	3-chlorocatechol degradation	40	2 of 5
[Ref.: #66794]	arachidonate biosynthesis	40	2 of 5
[Ref.: #66794]	arachidonic acid metabolism	38.89	7 of 18
[Ref.: #66794]	d-xylose degradation	36.36	4 of 11
[Ref.: #66794]	degradation of pentoses	35.71	10 of 28
[Ref.: #66794]	phenol degradation	35	7 of 20
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	molybdenum cofactor biosynthesis	33.33	3 of 9
[Ref.: #66794]	3-phenylpropionate degradation	33.33	5 of 15
[Ref.: #66794]	4-hydroxymandelate degradation	33.33	3 of 9
[Ref.: #66794]	enterobactin biosynthesis	33.33	1 of 3
[Ref.: #66794]	degradation of hexoses	33.33	6 of 18
[Ref.: #66794]	cyanate degradation	33.33	1 of 3
[Ref.: #66794]	sulfoquinovose degradation	33.33	1 of 3
[Ref.: #66794]	valine metabolism	33.33	3 of 9
[Ref.: #66794]	selenocysteine biosynthesis	33.33	2 of 6
[Ref.: #66794]	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
[Ref.: #66794]	ascorbate metabolism	31.82	7 of 22
[Ref.: #66794]	androgen and estrogen metabolism	31.25	5 of 16
[Ref.: #66794]	sulfate reduction	30.77	4 of 13
[Ref.: #66794]	myo-inositol biosynthesis	30	3 of 10
[Ref.: #66794]	aclacinomycin biosynthesis	28.57	2 of 7
[Ref.: #66794]	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
[Ref.: #66794]	butanoate fermentation	25	1 of 4
[Ref.: #66794]	biotin biosynthesis	25	1 of 4
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	carnitine metabolism	25	2 of 8
[Ref.: #66794]	degradation of sugar alcohols	25	4 of 16
[Ref.: #66794]	catecholamine biosynthesis	25	1 of 4
[Ref.: #66794]	polyamine pathway	21.74	5 of 23
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43119]	Sample type/isolated from	sediment sample from a marine solar saltern
[Ref.: #43119]	Geographic location (country and/or sea, region)	Weihai coast, Shandong Province
[Ref.: #43119]	Country	China
[Ref.: #43119]	Country ISO 3 Code	CHN
[Ref.: #43119]	Continent	Asia
[Ref.: #43119]	Geographic location	36.0000°/122.0000°
[Ref.: #43119]	Enrichment culture	modified marine agar 2216 (MA; BD Difco)
[Ref.: #43119]	Enrichment culture duration	5-7 days
[Ref.: #43119]	Enrichment culture temperature	37 ̊C
[Ref.: #43119]	Isolation/enrichment procedure	serial dilution plating method
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment
#Condition	#Saline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KU954547 (>99% sequence identity) for Longibacter salinarum subclade from Microbeatlas

Aquatic Samples	3
Soil Samples	2
Animal Samples
Plant Samples
Total Samples	5

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43119]	Rhodothermaceae bacterium WDS2C18 16S ribosomal RNA gene, partial sequence	KU954547	1456	GenBank	1850348 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Longibacter salinarum KCTC 52045	GCA_002554795	contig	GenBank	1850348 tax ID	*
[Ref.: #66792]	Longibacter salinarum strain KCTC 52045	1850348.3	wgs	PATRIC	1850348 tax ID	*
[Ref.: #66792]	Longibacter salinarum KCTC 52045	2788499893	draft	JGI IMG/M	1850348 tax ID	*

[Ref.: #43119]

GC-content

58.1 mol%

Thermal denaturation, fluorometry

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Longibacter salinarum strain KCTC 52045 PATRIC: 1850348.3
[Ref.: #69481]	spore-forming	no	98	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Longibacter salinarum strain KCTC 52045 PATRIC: 1850348.3
[Ref.: #69480]	motile	no	92.997	yes
[Ref.: #69480]	gram-positive	no	96.663	no
[Ref.: #69480]	anaerobic	no	98.001	yes
[Ref.: #69480]	aerobic	yes	68.903	yes
[Ref.: #69480]	halophile	yes	86.219	yes
[Ref.: #69480]	spore-forming	no	89.06	no
[Ref.: #69480]	glucose-util	yes	88.397	no
[Ref.: #69480]	flagellated	no	96.508	yes
[Ref.: #69480]	thermophile	no	68.254	yes
[Ref.: #69480]	glucose-ferment	no	83.132	no

Availability in culture collections External links

[Ref.: #43119]

Culture collection no.

KCTC 52045, MCCC 1H00132

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Draft Genome Sequences of Longimonas halophila KCTC 42399 and Longibacter salinarum KCTC 52045.	Liew KJ, Teo SC, Shamsir MS, Goh KM	Microbiol Resour Announc	10.1128/MRA.01238-19	2019	*
Phylogeny	Longibacter salinarum gen. nov., sp. nov., isolated from a marine solar saltern.	Xia J, Dunlap CA, Flor-Weiler L, Rooney AP, Chen GJ, Du ZJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.001190	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43119

Jun Xia, Christopher A. Dunlap, Lina Flor-Weiler, Alejandro P. Rooney, Guan-Jun Chen, Zong-Jun Du: Longibacter salinarum gen. nov., sp. nov., isolated from a marine solar saltern. IJSEM 66: 3287 - 3292 2016 ( DOI 10.1099/ijsem.0.001190 , PubMed 27226210 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Longibacter salinarum strain KCTC 52045

Longibacter salinarum strain KCTC 52045

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