Name: Actinomadura montaniterrae CYP1-1B
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140490

Type strain:

Species: Actinomadura montaniterrae

Strain Designation: CYP1-1B

Culture col. no.: JCM 16995, KCTC 39784, PCU 349, TISTR 2400

Strain history: <- Somboon Tanasupawat

NCBI tax ID(s): 1803903 (species)

Section

Actinomadura montaniterrae CYP1-1B is an aerobe, spore-forming, Gram-positive bacterium that builds a substrate mycelium and was isolated from mountain soil sample.

Gram-positive
spore-forming
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Streptosporangiales*
	Family	*Thermomonosporaceae*
	Genus	*Actinomadura*
	Species	**Actinomadura montaniterrae**
	Full Scientific Name (LPSN)	Actinomadura montaniterrae Songsumanus et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43102]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #43102]	Colony color	dark greyish yellow
[Ref.: #43102]	Cultivation medium used	ISP 2

[Ref.: #43102]	Multicellular complex forming ability	yes
[Ref.: #43102]	Multicellular complex name	substrate mycelium
[Ref.: #43102]	Multicellular complex color	dark greyish yellow
[Ref.: #43102]	Medium Name (multicellularity)	ISP2
[Ref.: #43102]	Multicellular complex further description	extensively branched non-fragmenting substrate mycelium

[Ref.: #43102]	Multicellular complex forming ability	yes
[Ref.: #43102]	Multicellular complex name	aerial mycelium
[Ref.: #43102]	Multicellular complex color	pale green
[Ref.: #43102]	Medium Name (multicellularity)	ISP2

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43102]

Culture medium

ISP 7

[Ref.: #43102]

Culture medium growth

[Ref.: #43102]

Culture medium

nutritient agar (NA)

[Ref.: #43102]

Culture medium growth

[Ref.: #43102]

Culture medium

ISP 2

[Ref.: #43102]

Culture medium growth

		Temperature
[Ref.: #43102]	growth	20-45 °C
[Ref.: #67770]	growth	28 °C
[Ref.: #67771]	growth	28 °C

[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43102]			growth	4.5-8.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43102]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #43102]	Spore description	oval to ellipsoid spores formed along the sporogenous hyphae and its branches
[Ref.: #43102]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43102]		NaCl		maximum	5	%(w/v)

[Ref.: #67770]

Observation

quinones: MK-9(H₆), MK-9(H₈), MK-9(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43102]	cellobiose ChEBI	+	carbon source
[Ref.: #43102]	D-fructose ChEBI	+	carbon source
[Ref.: #43102]	D-galactose ChEBI	-	carbon source
[Ref.: #43102]	D-glucose ChEBI	+	carbon source
[Ref.: #43102]	D-mannitol ChEBI	+	carbon source
[Ref.: #43102]	D-ribose ChEBI	-	carbon source
[Ref.: #43102]	D-xylose ChEBI	+	carbon source
[Ref.: #43102]	gelatin ChEBI	-	hydrolysis
[Ref.: #43102]	glycerol ChEBI	-	carbon source
[Ref.: #43102]	L-arabinose ChEBI	+	carbon source
[Ref.: #43102]	L-rhamnose ChEBI	+	carbon source
[Ref.: #43102]	lactose ChEBI	+	carbon source
[Ref.: #43102]	melibiose ChEBI	-	carbon source
[Ref.: #43102]	milk	-	hydrolysis
[Ref.: #43102]	nitrate ChEBI	-	reduction
[Ref.: #43102]	raffinose ChEBI	-	carbon source
[Ref.: #43102]	salicin ChEBI	-	carbon source
[Ref.: #43102]	starch ChEBI	+	hydrolysis
[Ref.: #43102]	sucrose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43102]

Fatty acid	Percentage
C16:0	31.2
C18:0 10-methyl	19
C18:1ω9c	17.1
C16:0 2OH	10.5
C16:1ω7c	5.4
C14:0	4.6
C18:0	3.3
C16:0 10-methyl	1.9
C17:0	0.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43102]	Sample type/isolated from	mountain soil sample
[Ref.: #43102]	Geographic location (country and/or sea, region)	Chaiyaphum Province
[Ref.: #43102]	Country	Thailand
[Ref.: #43102]	Country ISO 3 Code	THA
[Ref.: #43102]	Continent	Asia
[Ref.: #43102]	Enrichment culture	starch casein nitrate agar
[Ref.: #43102]	Enrichment culture composition	supplemented with nystatin (50 mg/l) and nalidixic acid (20 mg/l)
[Ref.: #43102]	Enrichment culture duration	21 days
[Ref.: #43102]	Enrichment culture temperature	30 ̊C

[Ref.: #67770]	Sample type/isolated from	Mountain soil
[Ref.: #67770]	Country	Thailand
[Ref.: #67770]	Country ISO 3 Code	THA
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From Mountain soil
[Ref.: #67771]	Country	Thailand
[Ref.: #67771]	Country ISO 3 Code	THA
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC126428 (>99% sequence identity) for Actinomadura from Microbeatlas

Aquatic Samples	10
Soil Samples	69
Animal Samples	7
Plant Samples	7
Total Samples	93

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43102]	Actinomadura sp. CYP1-1B gene for 16S ribosomal RNA, partial sequence	LC126428	1488	GenBank	1803903 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Actinomadura montaniterrae CYP1-1B	GCA_008923365	scaffold	GenBank	1803903 tax ID	*
[Ref.: #66792]	Actinomadura montaniterrae CYP1-1B	2898631135	draft	JGI IMG/M	1803903 tax ID	*

[Ref.: #43102]

GC-content

74.3 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Actinomadura montaniterrae CYP1-1B NCBI: GCA_008923365.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Actinomadura montaniterrae CYP1-1B NCBI: GCA_008923365.1
[Ref.: #69480]	motile	no	92.066	no
[Ref.: #69480]	flagellated	no	98.318	no
[Ref.: #69480]	gram-positive	yes	88.261	no
[Ref.: #69480]	anaerobic	no	98.692	yes
[Ref.: #69480]	aerobic	yes	89.553	yes
[Ref.: #69480]	halophile	no	93.076	no
[Ref.: #69480]	spore-forming	yes	92.772	yes
[Ref.: #69480]	thermophile	no	97.14	yes
[Ref.: #69480]	glucose-util	yes	88.5	yes
[Ref.: #69480]	glucose-ferment	no	88.663	no

Availability in culture collections External links

[Ref.: #43102]

Culture collection no.

JCM 16995, KCTC 39784, PCU 349, TISTR 2400

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Actinomadura violacea sp. nov., a madurastatin A1-producing strain isolated from lichen in Thailand.	Kanchanasin P, Phongsopitanun W, Yuki M, Kudo T, Ohkuma M, Nakashima T, Tanasupawat S	Int J Syst Evol Microbiol	10.1099/ijsem.0.005126	2021	*
Phylogeny	Actinomadura physcomitrii sp. nov., a novel actinomycete isolated from moss [Physcomitrium sphaericum (Ludw) Fuernr].	Zhuang X, Peng C, Wang Z, Zhao J, Shen Y, Liu C, Xiang W	Antonie Van Leeuwenhoek	10.1007/s10482-019-01380-7	2020	*
Phylogeny	Actinomadura montaniterrae sp. nov., isolated from mountain soil.	Songsumanus A, Kudo T, Ohkuma M, Phongsopitanun W, Tanasupawat S	Int J Syst Evol Microbiol	10.1099/ijsem.0.001196	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43102

Apakorn Songsumanus, Takuji Kudo, Moriya Ohkuma, Wongsakorn Phongsopitanun, Somboon Tanasupawat: Actinomadura montaniterrae sp. nov., isolated from mountain soil. IJSEM 66: 3310 - 3316 2016 ( DOI 10.1099/ijsem.0.001196 , PubMed 27255441 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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