Name: Paenibacillus lacus Agd-32
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140459

Type strain:

Species: Paenibacillus lacus

Strain Designation: Agd-32

Culture col. no.: BCRC 80902, KCTC 33691, LMG 28836

Strain history: <- Wen-Ming Chen, National Kaohsiung Marine Univ.

NCBI tax ID(s): 1615953 (species)

Section

Paenibacillus lacus Agd-32 is an aerobe, spore-forming, mesophilic bacterium that forms circular colonies and was isolated from water.

colony-forming
Gram-variable
motile
rod-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Paenibacillus*
	Species	**Paenibacillus lacus**
	Full Scientific Name (PNU)	Paenibacillus lacus Chen et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43197]	Gram stain	variable
[Ref.: #43197]	Cell length	3.0-4.0 µm
[Ref.: #43197]	Cell width	0.5-1.0 µm
[Ref.: #43197]	Cell shape	rod-shaped
[Ref.: #43197]	Motility	yes
[Ref.: #43197]	Flagellum arrangement	peritrichous

[Ref.: #67771]	Gram stain	variable

[Ref.: #67771]	Cell shape	rod-shaped
[Ref.: #67771]	Motility	yes
[Ref.: #67771]	Flagellum arrangement	peritrichous

[Ref.: #43197]	Colony size	0.5-2.0 mm
[Ref.: #43197]	Colony color	non-pigmented
[Ref.: #43197]	Colony shape	circular
[Ref.: #43197]	Incubation period	2 days
[Ref.: #43197]	Cultivation medium used	R2A

Information on culture and growth conditions Culture and growth conditions

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Culture medium

Reasoner's 2A agar (R2A)

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Culture medium growth

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Culture medium

nutritient agar (NA)

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Culture medium growth

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Culture medium

trypticase soy agar (TSA)

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Culture medium growth

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Culture medium

Luria Bertani (LB) agar

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Culture medium growth

		Temperature
[Ref.: #43197]	optimum	37 °C
[Ref.: #43197]	growth	25-40 °C
[Ref.: #67771]	growth	30 °C

[Ref.: #43197]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43197]			optimum	7
[Ref.: #43197]			growth	7-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43197]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #43197]	Spore description	ellipsoidal in swollen sporangia
[Ref.: #43197]	Type of spore	endospore
[Ref.: #43197]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43197]		NaCl		growth	0-3	%
[Ref.: #43197]		NaCl		optimum	2	%

[Ref.: #43197]	Observation	cells are surrounded by a thick capsule
[Ref.: #67771]	Observation	quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43197]	2-oxogluconate	-	builds acid from
[Ref.: #43197]	5-dehydro-D-gluconate ChEBI	+
[Ref.: #43197]	alginate ChEBI	-	hydrolysis
[Ref.: #43197]	amygdalin ChEBI	-	builds acid from
[Ref.: #43197]	arbutin ChEBI	+
[Ref.: #43197]	carboxymethylcellulose ChEBI	+	hydrolysis
[Ref.: #43197]	casein	-	hydrolysis
[Ref.: #43197]	cellobiose ChEBI	+
[Ref.: #43197]	chitin ChEBI	-	hydrolysis
[Ref.: #43197]	corn oil	-	hydrolysis
[Ref.: #43197]	D-arabinose ChEBI	+
[Ref.: #43197]	D-arabitol ChEBI	-	builds acid from
[Ref.: #43197]	D-fructose ChEBI	+
[Ref.: #43197]	D-fucose ChEBI	-	builds acid from
[Ref.: #43197]	D-galactose ChEBI	+
[Ref.: #43197]	D-glucose ChEBI	-	builds acid from
[Ref.: #43197]	D-glucose ChEBI	+
[Ref.: #43197]	D-lyxose ChEBI	+
[Ref.: #43197]	D-mannitol ChEBI	-	builds acid from
[Ref.: #43197]	D-mannose ChEBI	+
[Ref.: #43197]	D-ribose ChEBI	+
[Ref.: #43197]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #43197]	D-tagatose ChEBI	+
[Ref.: #43197]	D-xylose ChEBI	+
[Ref.: #43197]	dna ChEBI	-	hydrolysis
[Ref.: #43197]	erythritol ChEBI	-	builds acid from
[Ref.: #43197]	esculin ChEBI	+	hydrolysis
[Ref.: #43197]	esculin ChEBI	+
[Ref.: #43197]	galactitol ChEBI	-	builds acid from
[Ref.: #43197]	gelatin ChEBI	+	hydrolysis
[Ref.: #43197]	gentiobiose ChEBI	+
[Ref.: #43197]	gluconate ChEBI	-	builds acid from
[Ref.: #43197]	glycerol ChEBI	+	builds acid from
[Ref.: #43197]	glycogen ChEBI	-	builds acid from
[Ref.: #43197]	inulin ChEBI	-	builds acid from
[Ref.: #43197]	L-arabinose ChEBI	+
[Ref.: #43197]	L-arabitol ChEBI	-	builds acid from
[Ref.: #43197]	L-fucose ChEBI	+
[Ref.: #43197]	L-rhamnose ChEBI	+
[Ref.: #43197]	L-sorbose ChEBI	+	builds acid from
[Ref.: #43197]	L-xylose ChEBI	-	builds acid from
[Ref.: #43197]	lactose ChEBI	+
[Ref.: #43197]	lecithin ChEBI	-	hydrolysis
[Ref.: #43197]	maltose ChEBI	+
[Ref.: #43197]	melezitose ChEBI	-	builds acid from
[Ref.: #43197]	melibiose ChEBI	+
[Ref.: #43197]	methyl alpha-D-glucopyranoside ChEBI	+
[Ref.: #43197]	methyl alpha-D-mannoside ChEBI	+
[Ref.: #43197]	methyl beta-D-xylopyranoside ChEBI	+
[Ref.: #43197]	myo-inositol ChEBI	-	builds acid from
[Ref.: #43197]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #43197]	nitrate ChEBI	-	reduction
[Ref.: #43197]	raffinose ChEBI	+
[Ref.: #43197]	ribitol ChEBI	-	builds acid from
[Ref.: #43197]	salicin ChEBI	+
[Ref.: #43197]	starch ChEBI	-	hydrolysis
[Ref.: #43197]	starch ChEBI	+
[Ref.: #43197]	sucrose ChEBI	-	builds acid from
[Ref.: #43197]	trehalose ChEBI	-	builds acid from
[Ref.: #43197]	turanose ChEBI	-	builds acid from
[Ref.: #43197]	tween 20 ChEBI	-	hydrolysis
[Ref.: #43197]	tween 40 ChEBI	-	hydrolysis
[Ref.: #43197]	tween 60 ChEBI	-	hydrolysis
[Ref.: #43197]	tween 80 ChEBI	-	hydrolysis
[Ref.: #43197]	urea ChEBI	-	hydrolysis
[Ref.: #43197]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #43197]	ampicillin ChEBI	yes	10	µg (disc)
[Ref.: #43197]	chloramphenicol ChEBI	yes	30	µg (disc)
[Ref.: #43197]	gentamicin ChEBI	yes	10	µg (disc)
[Ref.: #43197]	kanamycin ChEBI	yes	30	µg (disc)
[Ref.: #43197]	nalidixic acid ChEBI	yes	30	µg (disc)
[Ref.: #43197]	novobiocin ChEBI	yes	30	µg (disc)
[Ref.: #43197]	penicillin g ChEBI	yes	10	Unit (disc)
[Ref.: #43197]	rifampicin ChEBI	yes	5	µg (disc)
[Ref.: #43197]	streptomycin ChEBI	yes	10	µg (disc)
[Ref.: #43197]	tetracycline ChEBI				yes	30	µg (disc)

	Antibiotica	Group ID of combined antibiotics	Metabolite	Antibiotic resistance	Concentration
[Ref.: #43197]		26	sulfamethoxazole ChEBI	yes	23.75	µg (disc)
[Ref.: #43197]		26	trimethoprim ChEBI	yes	1.25	µg (disc)

	Metabolite production	Metabolite
[Ref.: #43197]		indole ChEBI

	Enzyme	Enzyme activity	EC number
[Ref.: #43197]	acid phosphatase	+	3.1.3.2
[Ref.: #43197]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43197]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #43197]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43197]	alpha-galactosidase	+	3.2.1.22
[Ref.: #43197]	alpha-glucosidase	-	3.2.1.20
[Ref.: #43197]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43197]	arginine dihydrolase	-	3.5.3.6
[Ref.: #43197]	beta-galactosidase	+	3.2.1.23
[Ref.: #43197]	beta-glucosidase	+	3.2.1.21
[Ref.: #43197]	beta-glucuronidase	+	3.2.1.31
[Ref.: #43197]	catalase	+	1.11.1.6
[Ref.: #43197]	cystine arylamidase	-	3.4.11.3
[Ref.: #43197]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43197]	esterase (C 4)	+
[Ref.: #43197]	esterase Lipase (C 8)	+
[Ref.: #43197]	leucine arylamidase	+	3.4.11.1
[Ref.: #43197]	lipase (C 14)	-
[Ref.: #43197]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43197]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43197]	trypsin	-	3.4.21.4
[Ref.: #43197]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43197]

Fatty acid	Percentage
C15:0 anteiso	41.7
C16:0	20.7
C16:0 iso	9.6
C17:0 anteiso	4.8
C18:0	4.7
C14:0 iso	3.9
C14:0	3.2
C14:0 anteiso	2.6
C12:0	2.1
C15:0 iso	2
C17:0 iso	1.5
C16:1ω11c	1.2

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43197]	Sample type/isolated from	water
[Ref.: #43197]	Geographic location (country and/or sea, region)	Agongdian Reservoir, vicinity of Kaohsiung city
[Ref.: #43197]	Country	Taiwan
[Ref.: #43197]	Country ISO 3 Code	TWN
[Ref.: #43197]	Continent	Asia
[Ref.: #43197]	Geographic location	22.0000°/120.0000°
[Ref.: #43197]	Enrichment culture	R2A agar
[Ref.: #43197]	Enrichment culture duration	3 days
[Ref.: #43197]	Enrichment culture temperature	25 ̊C

[Ref.: #67771]	Sample type/isolated from	From a water sample taken from the Agongdian Reservoir in the vicinity of Kaohsiung city
[Ref.: #67771]	Country	Taiwan
[Ref.: #67771]	Country ISO 3 Code	TWN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LN812815 (>99% sequence identity) for Paenibacillus from Microbeatlas

Aquatic Samples	4
Soil Samples	131
Animal Samples	18
Plant Samples	13
Total Samples	166

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43197]	Paenibacillus sp. Agd-32 partial 16S rRNA gene, strain Agd-32	LN812815	1454	GenBank	1615953 tax ID

[Ref.: #43197]	GC-content	56 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67771]	GC-content	56.0 mol%

Availability in culture collections External links

[Ref.: #43197]

Culture collection no.

BCRC 80902, KCTC 33691, LMG 28836

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Paenibacillus lacus sp. nov., isolated from a water reservoir.	Chen WM, Lin KR, Sheu SY	Int J Syst Evol Microbiol	10.1099/ijsem.0.001768	2017	*
Phylogeny	Paenibacillus translucens sp. nov., isolated from tidal flat sediment.	Kim YS, Cha CJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.002613	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43197

Wen-Ming Chen, Kai-Rou Lin, Shih-Yi Sheu: Paenibacillus lacus sp. nov., isolated from a water reservoir. IJSEM 67: 1582 - 1588 2017 ( DOI 10.1099/ijsem.0.001768 , PubMed 28537545 )

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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