Name: Halalkalibacter kiskunsagensis B16-24
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140404

Type strain:

Species: Halalkalibacter kiskunsagensis

Strain Designation: B16-24

Culture col. no.: DSM 29791, NCAIM B.02610

Strain history: <- A. K. Borsodi, Eötvös Loránd Univ. Budapest, Hungary; B16-24

NCBI tax ID(s): 1548599 (species)

SI-ID 404948

DSM 29791

Section

Halalkalibacter kiskunsagensis B16-24 is a facultative anaerobe, moderately halophilic, spore-forming bacterium that forms circular colonies and was isolated from soda rhizosphere soil of the bayonet gras .

colony-forming
Gram-positive
rod-shaped
spore-forming
facultative anaerobe
moderately halophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Halalkalibacter*
	Species	**Halalkalibacter kiskunsagensis**
	Full Scientific Name (LPSN)	Halalkalibacter kiskunsagensis (Borsodi et al. 2017) Joshi et al. 2022

	Synonym	Alkalihalobacillus kiskunsagensis
	Synonym	Bacillus kiskunsagensis

Information on morphological and physiological properties Morphology

[Ref.: #43900]	Gram stain	positive

[Ref.: #43900]	Cell length	1.5-2.5 µm

[Ref.: #43900]	Cell width	0.5-0.7 µm

[Ref.: #43900]	Cell shape	rod-shaped

[Ref.: #43900]	Motility	no

[Ref.: #43900]	Colony color	creamish white
[Ref.: #43900]	Colony shape	circular
[Ref.: #43900]	Incubation period	1-2 days
[Ref.: #43900]	Cultivation medium used	Horikoshi alkaline medium

Information on culture and growth conditions Culture and growth conditions

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Culture medium

Horikoshi alkaline medium

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Culture medium growth

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Culture medium composition

expand / minimize

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Culture medium

Nutrient agar (NA)

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Culture medium growth

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Culture medium composition

expand / minimize

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Culture medium

Reasoner's 2A agar (R2A)

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Culture medium growth

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Culture medium composition

expand / minimize

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Culture medium

HORIKOSHI ALKALINE (DSMZ Medium 940)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #43011]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #43011]	growth	25 °C
[Ref.: #43900]	growth	10-40 °C
[Ref.: #43900]	optimum	25-28 °C

[Ref.: #43011]	Temperature range	mesophilic
[Ref.: #43900]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43900]			optimum	9.0-10.0
[Ref.: #43900]			growth	7.0-12.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43900]

Oxygen tolerance

facultative anaerobe

[Ref.: #43900]	Spore description	central, ellipsoidal endospores in slightly swollen sporangia
[Ref.: #43900]	Type of spore	endospore
[Ref.: #43900]	Ability of spore formation	yes

[Ref.: #43900]

Halophily / tolerance level

moderately halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43900]		NaCl		growth	5-10	%(w/v)
[Ref.: #43900]		NaCl		optimum	5-7	%(w/v)

[Ref.: #43900]

Observation

quinone MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43900]	asparagine ChEBI	-	carbon source
[Ref.: #43900]	casein	-	hydrolysis
[Ref.: #43900]	citrate ChEBI	-	carbon source
[Ref.: #43900]	D-fructose ChEBI	+	carbon source
[Ref.: #43900]	D-galactose ChEBI	-	carbon source
[Ref.: #43900]	D-glucose ChEBI	-	builds acid from
[Ref.: #43900]	D-glucose ChEBI	-	builds gas from
[Ref.: #43900]	D-glucose ChEBI	+	carbon source
[Ref.: #43900]	D-mannose ChEBI	-	carbon source
[Ref.: #43900]	D-rhamnose ChEBI	-	carbon source
[Ref.: #43900]	D-ribose ChEBI	-	carbon source
[Ref.: #43900]	D-xylose ChEBI	-	carbon source
[Ref.: #43900]	dl-alanine ChEBI	+	carbon source
[Ref.: #43900]	esculin ChEBI	+	hydrolysis
[Ref.: #43900]	galactitol ChEBI	-	carbon source
[Ref.: #43900]	gelatin ChEBI	-	hydrolysis
[Ref.: #43900]	glucose ChEBI	-	fermentation
[Ref.: #43900]	glycerol ChEBI	-	carbon source
[Ref.: #43900]	L-arabinose ChEBI	-	carbon source
[Ref.: #43900]	L-serine ChEBI	+	carbon source
[Ref.: #43900]	lactose ChEBI	-	carbon source
[Ref.: #43900]	maltose ChEBI	-	carbon source
[Ref.: #43900]	myo-inositol ChEBI	-	carbon source
[Ref.: #43900]	nitrate ChEBI	+	reduction
[Ref.: #43900]	sodium acetate ChEBI	-	carbon source
[Ref.: #43900]	sodium formate ChEBI	-	carbon source
[Ref.: #43900]	sodium pyruvate ChEBI	-	carbon source
[Ref.: #43900]	starch ChEBI	-	hydrolysis
[Ref.: #43900]	sucrose ChEBI	-	carbon source
[Ref.: #43900]	trehalose ChEBI	+	carbon source
[Ref.: #43900]	tween 80 ChEBI	-	hydrolysis
[Ref.: #43900]	urea ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #43900]	acetoin ChEBI	no
[Ref.: #43900]	hydrogen sulfide ChEBI	no
[Ref.: #43900]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #43900]		acetoin ChEBI	-
[Ref.: #43900]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #43900]	catalase	+	1.11.1.6
[Ref.: #43900]	cytochrome oxidase	-	1.9.3.1
[Ref.: #43900]	phosphatase	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43011]	Sample type/isolated from	soda rhizosphere soil of the bayonet gras (Bolboschoenus maritimus)
[Ref.: #43011]	Host species	Bolboschoenus maritimus
[Ref.: #43011]	Geographic location (country and/or sea, region)	Kiskunság National Park, Böddi-szék soda pond (N46° 46', E019° 08')
[Ref.: #43011]	Country	Hungary
[Ref.: #43011]	Country ISO 3 Code	HUN
[Ref.: #43011]	Continent	Europe
[Ref.: #43011]	Geographic location	46.7667°/19.1333°

[Ref.: #43900]	Sample type/isolated from	rhizosphere soil of bayonet grass Bolboschoenus maritimus on the shores of the Böddi-szék soda pond in the Kiskunság National Park, Hungary
[Ref.: #43900]	Sampling date	09-2013
[Ref.: #43900]	Geographic location (country and/or sea, region)	Böddi-szék soda pond in the Kiskunság National Park
[Ref.: #43900]	Country	Hungary
[Ref.: #43900]	Country ISO 3 Code	HUN
[Ref.: #43900]	Continent	Europe
[Ref.: #43900]	Geographic location	46.7667°/19.1333°
[Ref.: #43900]	Enrichment culture	Horikoshi alkaline medium 940
[Ref.: #43900]	Enrichment culture composition	10.0 g/L D-glucose, 5.0 g/L peptone, 5.0 g/L yeast extract, 1.0 g/L KH2PO4, 0.2 g/L MgSO4.7H2O, 5.0 g/L Na2CO3, 15.0 g/L agar, pH 9.0
[Ref.: #43900]	Enrichment culture duration	6 days
[Ref.: #43900]	Enrichment culture temperature	28 ̊C
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Condition	#Alkaline	-
#Condition	#Saline	-
#Environmental	#Terrestrial	#Soil
#Host Body-Site	#Plant	#Rhizosphere

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LN610501 (>99% sequence identity) for Alkalihalobacillus from Microbeatlas

Aquatic Samples	2
Soil Samples	19
Animal Samples	1
Plant Samples
Total Samples	22

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

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Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43011]	Bacillus sp. B16-24 partial 16S rRNA gene, strain B16-24	LN610501	1444	ENA	1548599 tax ID

[Ref.: #43900]

GC-content

36.6 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #43011]

Culture collection no.

DSM 29791, NCAIM B.02610

[Ref.: #96683]

SI-ID 404948

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bacillus suaedae sp. nov., isolated from the stem of Suaeda aralocaspica in north-west China.	Lei RF, Ma Q, Li YQ, Abudourousuli D, Wang S, Rouzi Z, Aosiman M, An DD, Li WJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.005362	2022	*
Phylogeny	Bacillus kiskunsagensis sp. nov., a novel alkaliphilic and moderately halophilic bacterium isolated from soda soil.	Borsodi AK, Toth E, Aszalos JM, Barany A, Schumann P, Sproer C, Kovacs AL, Marialigeti K, Szili-Kovacs T	Int J Syst Evol Microbiol	10.1099/ijsem.0.002149	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43011

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29791

#43900

Andrea K. Borsodi, Erika Tóth, Júlia M. Aszalós, Ágnes Bárány, Peter Schumann, Cathrin Spröer, Attila L. Kovács, Károly Márialigeti, Tibor Szili-Kovács: Bacillus kiskunsagensis sp. nov., a novel alkaliphilic and moderately halophilic bacterium isolated from soda soil. IJSEM 67: 3490 - 3495 2017 ( DOI 10.1099/ijsem.0.002149 , PubMed 28857030 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#96683

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID404948.1 )

* These data were automatically processed and therefore are not curated

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