Name: Desulfosarcina alkanivorans PL12
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 140361

Type strain:

Species: Desulfosarcina alkanivorans

Strain Designation: PL12

Culture col. no.: DSM 103901, JCM 31728

Strain history: M. Watanabe; Hokkaido Univ., Japan; PL12.

NCBI tax ID(s): 571177 (species)

SI-ID 400690

DSM 103901

Special Collections

Section

Desulfosarcina alkanivorans PL12 is an anaerobe, Gram-negative, motile bacterium that was isolated from oil-polluted marine sediment, p-xylene-degrading sulfate-reducing enrichment culture.

Gram-negative
motile
rod-shaped
anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Deltaproteobacteria*
	Order	*Desulfobacterales*
	Family	*Desulfobacteraceae*
	Genus	*Desulfosarcina*
	Species	**Desulfosarcina alkanivorans**
	Full Scientific Name (LPSN)	Desulfosarcina alkanivorans Watanabe et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43887]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %95.389

[Ref.: #43887]	Cell length	1.0-1.5 µm

[Ref.: #43887]	Cell width	0.5 µm

[Ref.: #43887]	Cell shape	rod-shaped

[Ref.: #43887]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43887]

Culture medium

Basal medium

[Ref.: #43887]

Culture medium growth

[Ref.: #43887]

Culture medium composition

expand / minimize

[Ref.: #42968]

Culture medium

DESULFOBACTER SP. MEDIUM (LACTATE) (DSMZ Medium 195c)

[Ref.: #42968]

Culture medium growth

[Ref.: #42968]

Culture medium link

Medium recipe at MediaDive

[Ref.: #42968]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #42968]	growth	30 °C
[Ref.: #43887]	growth	18-37 °C
[Ref.: #43887]	optimum	30-34 °C
[Ref.: #67770]	growth	30 °C

	pH	Kind of pH		pH
[Ref.: #43887]			optimum	7.0-7.3
[Ref.: #43887]			growth	6.5-7.3

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43887]

Oxygen tolerance

anaerobe

[Ref.: #43887]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43887]	acetate ChEBI	+	carbon source
[Ref.: #43887]	acetate ChEBI	+	electron donor
[Ref.: #43887]	benzene ChEBI	-	carbon source
[Ref.: #43887]	benzene ChEBI	-	electron donor
[Ref.: #43887]	benzoate ChEBI	-	carbon source
[Ref.: #43887]	benzoate ChEBI	-	electron donor
[Ref.: #43887]	butyrate ChEBI	+	carbon source
[Ref.: #43887]	butyrate ChEBI	+	electron donor
[Ref.: #43887]	citrate ChEBI	-	carbon source
[Ref.: #43887]	citrate ChEBI	-	electron donor
[Ref.: #43887]	decane ChEBI	+	carbon source
[Ref.: #43887]	decane ChEBI	+	electron donor
[Ref.: #43887]	elemental sulfur ChEBI	-	electron acceptor
[Ref.: #43887]	ethanol ChEBI	-	carbon source
[Ref.: #43887]	ethanol ChEBI	-	electron donor
[Ref.: #43887]	formate ChEBI	+	carbon source
[Ref.: #43887]	formate ChEBI	+	electron donor
[Ref.: #43887]	fumarate ChEBI	-	electron acceptor
[Ref.: #43887]	fumarate ChEBI	+	carbon source
[Ref.: #43887]	fumarate ChEBI	+	electron donor
[Ref.: #43887]	fumarate ChEBI	+	fermentation
[Ref.: #43887]	glucose ChEBI	-	carbon source
[Ref.: #43887]	glucose ChEBI	-	electron donor
[Ref.: #43887]	hexane ChEBI	+	carbon source
[Ref.: #43887]	hexane ChEBI	+	electron donor
[Ref.: #43887]	lactate ChEBI	+	carbon source
[Ref.: #43887]	lactate ChEBI	+	electron donor
[Ref.: #43887]	lactate ChEBI	+	fermentation
[Ref.: #43887]	malate ChEBI	+	fermentation
[Ref.: #43887]	methanol ChEBI	-	carbon source
[Ref.: #43887]	methanol ChEBI	-	electron donor
[Ref.: #43887]	nitrate ChEBI	-	electron acceptor
[Ref.: #43887]	phenol ChEBI	-	carbon source
[Ref.: #43887]	phenol ChEBI	-	electron donor
[Ref.: #43887]	propionate ChEBI	+	carbon source
[Ref.: #43887]	propionate ChEBI	+	electron donor
[Ref.: #43887]	pyruvate ChEBI	+	carbon source
[Ref.: #43887]	pyruvate ChEBI	+	electron donor
[Ref.: #43887]	succinate ChEBI	+	carbon source
[Ref.: #43887]	succinate ChEBI	+	electron donor
[Ref.: #43887]	sulfate ChEBI	+	electron acceptor
[Ref.: #43887]	sulfate ChEBI	+	electron donor
[Ref.: #43887]	sulfite ChEBI	+	electron acceptor
[Ref.: #43887]	thiosulfate ChEBI	+	electron acceptor
[Ref.: #43887]	toluene ChEBI	-	carbon source
[Ref.: #43887]	toluene ChEBI	-	electron donor
[Ref.: #43887]	xylene ChEBI	-	carbon source
[Ref.: #43887]	xylene ChEBI	-	electron donor
[Ref.: #43887]	yeast extract	+	carbon source
[Ref.: #43887]	yeast extract	+	electron donor
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43887]

Fatty acid	Percentage
C15:0	21.3
C16:0	17.8
C15:0 anteiso	11.6
C14:0	9.4
C16:1ω7c	7.6
C17:1ω6c	6
C15:1ω6c	5.9
C17:0	5.9
C17:1ω8c	4.7
C17:1ω8c	3.8
C15:0 DMA	1.8
C15:0 iso	1.6
C18:0	1.4
C16:1ω5c	1.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #42968]	Sample type/isolated from	oil-polluted marine sediment, p-xylene-degrading sulfate-reducing enrichment culture
[Ref.: #42968]	Geographic location (country and/or sea, region)	Shuaiba
[Ref.: #42968]	Country	Kuwait
[Ref.: #42968]	Country ISO 3 Code	KWT
[Ref.: #42968]	Continent	Asia

[Ref.: #43887]	Sample type/isolated from	p-xylene degarding sulfate-reducing enrichment culture established from petroleum-contaminated marine sediments of Shuaiba, Kuwait
[Ref.: #43887]	Geographic location (country and/or sea, region)	Shuaiba
[Ref.: #43887]	Country	Kuwait
[Ref.: #43887]	Country ISO 3 Code	KWT
[Ref.: #43887]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Oil-polluted marine sediments of Shuaiba
[Ref.: #67770]	Country	Kuwait
[Ref.: #67770]	Country ISO 3 Code	KWT
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Laboratory	#Lab enrichment
#Engineered	#Contamination	#Oil (Fuel)
#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB468588 (>99% sequence identity) for Desulfosarcina alkanivorans subclade from Microbeatlas

Aquatic Samples	110
Soil Samples	4
Animal Samples	2
Plant Samples	6
Total Samples	122

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #42968]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #42968]	Desulfosarcina alkanivorans gene for 16S ribosomal RNA, partial sequence	AB468588	1526	GenBank ENA	571177 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Desulfosarcina alkanivorans PL12	GCA_009688945	complete	GenBank ENA	571177 tax ID	*
[Ref.: #66792]	Desulfosarcina alkanivorans strain PL12	571177.3	complete	PATRIC	571177 tax ID	*

[Ref.: #42968]

GC-content

57 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Desulfosarcina alkanivorans PL12 NCBI: GCA_009688945.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Desulfosarcina alkanivorans PL12 NCBI: GCA_009688945.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	95.389	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	yes	75.166	yes
[Ref.: #69480]	aerobic	aerobicⓘ	no	78.02	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	81.487	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	86.736	no
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	66.822	no

Availability in culture collections External links

[Ref.: #42968]

Culture collection no.

DSM 103901, JCM 31728

[Ref.: #96659]

SI-ID 400690

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Desulfosarcina widdelii sp. nov. and Desulfosarcina alkanivorans sp. nov., hydrocarbon-degrading sulfate-reducing bacteria isolated from marine sediment and emended description of the genus Desulfosarcina.	Watanabe M, Higashioka Y, Kojima H, Fukui M	Int J Syst Evol Microbiol	10.1099/ijsem.0.002062	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#42968

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 103901

#43887

Miho Watanabe, Yuriko Higashioka, Hisaya Kojima, Manabu Fukui: Desulfosarcina widdelii sp. nov. and Desulfosarcina alkanivorans sp. nov., hydrocarbon-degrading sulfate-reducing bacteria isolated from marine sediment and emended description of the genus Desulfosarcina. IJSEM 67: 2994 - 2997 2017 ( DOI 10.1099/ijsem.0.002062 , PubMed 28820122 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#96659

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID400690.1 )

* These data were automatically processed and therefore are not curated

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