Desulfosarcina alkanivorans (DSM 103901, JCM 31728, PL12)

Name: Desulfosarcina alkanivorans (DSM 103901, JCM 31728, PL12)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 140361

Type strain

Strain Designation PL12

Culture col. no. DSM 103901 JCM 31728 PL12

NCBI tax ID(s) 571177

Special Collections DSMZ JCM Literature strains

History <- M. Watanabe, Hokkaido Univ., Inst. Low Temperature Sci., Sapporo, Japan; PL12 <- Y. Higashioka; {2008} M. Watanabe; Hokkaido Univ., Japan; PL12.

Links

Desulfosarcina alkanivorans PL12 is an anaerobe, Gram-negative, motile bacterium that was isolated from oil-polluted marine sediment, p-xylene-degrading sulfate-reducing enrichment culture.

Gram-negative motile rod-shaped anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 400690

JCM 31728,DSM 103901

@ref 20215

Last LPSN update 2026-02-09 11:33:36

Domain Bacteria

Phylum Thermodesulfobacteriota

Class Desulfobacteria

Order Desulfobacterales

Family Desulfosarcinaceae

Genus Desulfosarcina

Species Desulfosarcina alkanivorans

Full scientific name Desulfosarcina alkanivorans Watanabe et al. 2017

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
43887	negative	1.0-1.5 µm	0.5 µm	rod-shaped
125439	negative				99.7
125438	negative				95.389

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
43887	Basal medium			defined bicarbonate-buffered, sulfate-reduced saltwater medium
42968	DESULFOBACTER SP. MEDIUM (LACTATE) (DSMZ Medium 195c)		Medium recipe at MediaDive	Name: DESULFOBACTER SP. MEDIUM (LACTATE) (DSMZ Medium 195c) Composition: NaCl 20.9372 g/l Na2SO4 2.99103 g/l MgCl2 x 6 H2O 2.99103 g/l Na-L-lactate 2.49252 g/l Na2CO3 1.49551 g/l KCl 0.498504 g/l Na2S x 9 H2O 0.398804 g/l NH4Cl 0.299103 g/l KH2PO4 0.199402 g/l CaCl2 x 2 H2O 0.149551 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l NaOH 0.000498504 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l Pyridoxine hydrochloride 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Biotin 1.99402e-05 g/l Folic acid 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
42968	positive	growth	30
43887	positive	growth	18-37
43887	positive	optimum	30-34
67770	positive	growth	30

Culture pH

@ref	Ability	Type	PH
43887	positive	optimum	7.0-7.3
43887	positive	growth	6.5-7.3

Physiology and metabolism

Oxygen tolerance

43887

Oxygen toleranceanaerobe

Spore formation

@ref	Spore formation	Confidence
43887
125439		97.2

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43887	30089 ChEBI	acetate	+	carbon source
43887	30089 ChEBI	acetate	+	electron donor
43887	16716 ChEBI	benzene	-	carbon source
43887	16716 ChEBI	benzene	-	electron donor
43887	16150 ChEBI	benzoate	-	carbon source
43887	16150 ChEBI	benzoate	-	electron donor
43887	17968 ChEBI	butyrate	+	carbon source
43887	17968 ChEBI	butyrate	+	electron donor
43887	16947 ChEBI	citrate	-	carbon source
43887	16947 ChEBI	citrate	-	electron donor
43887	41808 ChEBI	decane	+	carbon source
43887	41808 ChEBI	decane	+	electron donor
43887	33403 ChEBI	elemental sulfur	-	electron acceptor
43887	16236 ChEBI	ethanol	-	carbon source
43887	16236 ChEBI	ethanol	-	electron donor
43887	15740 ChEBI	formate	+	carbon source
43887	15740 ChEBI	formate	+	electron donor
43887	29806 ChEBI	fumarate	-	electron acceptor
43887	29806 ChEBI	fumarate	+	carbon source
43887	29806 ChEBI	fumarate	+	electron donor
43887	29806 ChEBI	fumarate	+	fermentation
43887	17234 ChEBI	glucose	-	carbon source
43887	17234 ChEBI	glucose	-	electron donor
43887	29021 ChEBI	hexane	+	carbon source
43887	29021 ChEBI	hexane	+	electron donor
43887	24996 ChEBI	lactate	+	carbon source
43887	24996 ChEBI	lactate	+	electron donor
43887	24996 ChEBI	lactate	+	fermentation
43887	25115 ChEBI	malate	+	fermentation
43887	17790 ChEBI	methanol	-	carbon source
43887	17790 ChEBI	methanol	-	electron donor
43887	17632 ChEBI	nitrate	-	electron acceptor
43887	15882 ChEBI	phenol	-	carbon source
43887	15882 ChEBI	phenol	-	electron donor
43887	17272 ChEBI	propionate	+	carbon source
43887	17272 ChEBI	propionate	+	electron donor
43887	15361 ChEBI	pyruvate	+	carbon source
43887	15361 ChEBI	pyruvate	+	electron donor
43887	30031 ChEBI	succinate	+	carbon source
43887	30031 ChEBI	succinate	+	electron donor
43887	16189 ChEBI	sulfate	+	electron acceptor
43887	16189 ChEBI	sulfate	+	electron donor
43887	17359 ChEBI	sulfite	+	electron acceptor
43887	16094 ChEBI	thiosulfate	+	electron acceptor
43887	17578 ChEBI	toluene	-	carbon source
43887	17578 ChEBI	toluene	-	electron donor
43887	27338 ChEBI	xylene	-	carbon source
43887	27338 ChEBI	xylene	-	electron donor
43887		yeast extract	+	carbon source
43887		yeast extract	+	electron donor

Fatty acid profile

Metadata FA analysis

@ref

43887

Fatty acid	Percentage
C15:0	21.3
C16:0	17.8
C15:0 anteiso	11.6
C14:0	9.4
C16:1ω7c	7.6
C17:1ω6c	6
C15:1ω6c	5.9
C17:0	5.9
C17:1ω8c	4.7
C17:1ω8c	3.8
C15:0 DMA	1.8
C15:0 iso	1.6
C18:0	1.4
C16:1ω5c	1.1

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Laboratory	#Lab enrichment
#Engineered	#Contamination	#Oil (Fuel)
#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
42968	oil-polluted marine sediment, p-xylene-degrading sulfate-reducing enrichment culture	Shuaiba	Kuwait	KWT	Asia
43887	p-xylene degarding sulfate-reducing enrichment culture established from petroleum-contaminated marine sediments of Shuaiba, Kuwait	Shuaiba	Kuwait	KWT	Asia
67770	Oil-polluted marine sediments of Shuaiba		Kuwait	KWT	Asia

Taxonmaps

69479

Global distribution of 16S sequence AB468588 (>99% sequence identity) for Desulfosarcina alkanivorans subclade from Microbeatlas

Aquatic Samples	110
Soil Samples	4
Animal Samples	2
Plant Samples	6
Total Samples	122

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
42968	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM968894v1 assembly for Desulfosarcina alkanivorans PL12	complete	GCA_009688945	571177.3	8048255048	571177	93.44

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
42968	Desulfosarcina alkanivorans gene for 16S ribosomal RNA, partial sequence	AB468588	1526		571177

GC content

42968

GC-content (mol%)57

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Desulfosarcina alkanivorans strain PL12
PATRIC: 571177.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	97.20	no
125439	motility	BacteriaNetⓘ	no	53.30	no
125439	gram_stain	BacteriaNetⓘ	negative	99.70	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate anaerobe	88.60	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Desulfosarcina alkanivorans PL12
NCBI: GCA_009688945.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	95.39	yes
125438	anaerobic	anaerobicⓘ	yes	75.17	yes
125438	aerobic	aerobicⓘ	no	78.02	yes
125438	spore-forming	spore-formingⓘ	no	81.49	yes
125438	thermophilic	thermophileⓘ	no	86.74	no
125438	flagellated	motile2+ⓘ	yes	66.82	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Desulfosarcina widdelii sp. nov. and Desulfosarcina alkanivorans sp. nov., hydrocarbon-degrading sulfate-reducing bacteria isolated from marine sediment and emended description of the genus Desulfosarcina.	Watanabe M, Higashioka Y, Kojima H, Fukui M	Int J Syst Evol Microbiol	10.1099/ijsem.0.002062	2017

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#42968	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 103901
#43887	Miho Watanabe, Yuriko Higashioka, Hisaya Kojima, Manabu Fukui: Desulfosarcina widdelii sp. nov. and Desulfosarcina alkanivorans sp. nov., hydrocarbon-degrading sulfate-reducing bacteria isolated from marine sediment and emended description of the genus Desulfosarcina. IJSEM 67: 2994 - 2997 2017 ( DOI 10.1099/ijsem.0.002062 , PubMed 28820122 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Desulfosarcina alkanivorans (DSM 103901, JCM 31728, PL12)

Name and taxonomic classification