Name: Bacillus wudalianchiensis FJAT-27215
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140354

Type strain:

Species: Bacillus wudalianchiensis

Strain Designation: FJAT-27215

Culture col. no.: DSM 100757, CCTCC AB 2015266

Strain history: <- G. Liu, Fujian Academy of Agricultural Sciences, Fuzhou, China; FJAT-27215

NCBI tax ID(s): 1743143 (species)

SI-ID 407339

DSM 100757

Section

Bacillus wudalianchiensis FJAT-27215 is an aerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from volcanogenic soil.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus wudalianchiensis**
	Full Scientific Name (LPSN)	Bacillus wudalianchiensis Liu et al. 2017

Synonym

"Pseudobacillus wudalianchiensis"

Information on morphological and physiological properties Morphology

[Ref.: #43454]	Gram stain	positive

[Ref.: #43454]	Cell length	1.2-2.5 µm

[Ref.: #43454]	Cell width	0.7-0.8 µm

[Ref.: #43454]	Cell shape	rod-shaped

[Ref.: #43454]	Motility	yes

[Ref.: #43454]	Flagellum arrangement	polar

[Ref.: #43454]	Colony size	2-3 mm
[Ref.: #43454]	Colony color	light yellow
[Ref.: #43454]	Colony shape	circular
[Ref.: #43454]	Incubation period	2 days
[Ref.: #43454]	Cultivation medium used	Luria Bertani agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43454]

Culture medium

LB (Luria-Bertani) MEDIUM

[Ref.: #43454]

Culture medium growth

[Ref.: #42961]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #42961]

Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #42961]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #42961]	growth	30 °C
[Ref.: #43454]	growth	10-60 °C
[Ref.: #43454]	optimum	30 °C

[Ref.: #42961]	Temperature range	mesophilic
[Ref.: #43454]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43454]			optimum	7
[Ref.: #43454]			growth	5-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43454]

Oxygen tolerance

aerobe

[Ref.: #43454]	Spore description	ellipsoidal, central position in slightly swollen sporangia
[Ref.: #43454]	Type of spore	endospore
[Ref.: #43454]	Ability of spore formation	yes

[Ref.: #43454]

Halophily / tolerance level

non-halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43454]		NaCl		growth	0-3	%
[Ref.: #43454]		NaCl		optimum	0	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43454]	2-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #43454]	amygdalin ChEBI	-	builds acid from
[Ref.: #43454]	amygdalin ChEBI	-	carbon source
[Ref.: #43454]	amygdalin ChEBI	-	nitrogen source
[Ref.: #43454]	arabinose ChEBI	-	carbon source
[Ref.: #43454]	arabinose ChEBI	-	nitrogen source
[Ref.: #43454]	citrate ChEBI	+	assimilation
[Ref.: #43454]	esculin ChEBI	-	builds acid from
[Ref.: #43454]	gelatin ChEBI	-	hydrolysis
[Ref.: #43454]	gentiobiose ChEBI	-	builds acid from
[Ref.: #43454]	glucose ChEBI	-	builds acid from
[Ref.: #43454]	glucose ChEBI	-	carbon source
[Ref.: #43454]	glucose ChEBI	-	nitrogen source
[Ref.: #43454]	glycerol ChEBI	-	builds acid from
[Ref.: #43454]	L-arabinose ChEBI	-	builds acid from
[Ref.: #43454]	mannitol ChEBI	-	builds acid from
[Ref.: #43454]	mannose ChEBI	-	carbon source
[Ref.: #43454]	mannose ChEBI	-	nitrogen source
[Ref.: #43454]	melezitose ChEBI	-	carbon source
[Ref.: #43454]	melezitose ChEBI	-	nitrogen source
[Ref.: #43454]	melibiose ChEBI	-	builds acid from
[Ref.: #43454]	myo-inositol ChEBI	-	builds acid from
[Ref.: #43454]	myo-inositol ChEBI	-	carbon source
[Ref.: #43454]	myo-inositol ChEBI	-	nitrogen source
[Ref.: #43454]	nitrate ChEBI	+	reduction
[Ref.: #43454]	rhamnose ChEBI	-	carbon source
[Ref.: #43454]	rhamnose ChEBI	-	nitrogen source
[Ref.: #43454]	ribose ChEBI	-	builds acid from
[Ref.: #43454]	salicin ChEBI	-	builds acid from
[Ref.: #43454]	sorbitol ChEBI	-	builds acid from
[Ref.: #43454]	sorbose ChEBI	-	carbon source
[Ref.: #43454]	sorbose ChEBI	-	nitrogen source
[Ref.: #43454]	sucrose ChEBI	-	builds acid from
[Ref.: #43454]	sucrose ChEBI	-	carbon source
[Ref.: #43454]	sucrose ChEBI	-	nitrogen source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #43454]	acetoin ChEBI	no
[Ref.: #43454]	hydrogen sulfide ChEBI	no
[Ref.: #43454]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #43454]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #43454]	arginine dihydrolase	+	3.5.3.6
[Ref.: #43454]	beta-galactosidase	-	3.2.1.23
[Ref.: #43454]	catalase	+	1.11.1.6
[Ref.: #43454]	cytochrome oxidase	-	1.9.3.1
[Ref.: #43454]	lysine decarboxylase	-	4.1.1.18
[Ref.: #43454]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #43454]	tryptophan deaminase	-	4.1.99.1
[Ref.: #43454]	urease	+	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #42961]	Sample type/isolated from	volcanogenic soil
[Ref.: #42961]	Geographic location (country and/or sea, region)	Heilongjiang province, Wudalianchi
[Ref.: #42961]	Country	China
[Ref.: #42961]	Country ISO 3 Code	CHN
[Ref.: #42961]	Continent	Asia

[Ref.: #43454]	Sample type/isolated from	grass soil
[Ref.: #43454]	Geographic location (country and/or sea, region)	Wudalianchi, Heilongjiang Province
[Ref.: #43454]	Country	China
[Ref.: #43454]	Country ISO 3 Code	CHN
[Ref.: #43454]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Volcanic
#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Herbaceous plants (Grass,Crops)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KT362901 (>99% sequence identity) for Bacillus wudalianchiensis subclade from Microbeatlas

Aquatic Samples	135
Soil Samples	675
Animal Samples	398
Plant Samples	72
Total Samples	1280

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #42961]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #42961]	Bacillus wudalianchiensis strain FJAT-27215 16S ribosomal RNA gene, partial sequence	KT362901	1553	ENA	1743143 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Bacillus wudalianchiensis FJAT-27215	GCA_001685015	contig	GenBank	1743143 tax ID	*

[Ref.: #43454]	GC-content	41.18 mol%
[Ref.: #42961]	GC-content	41.2 mol%	sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bacillus wudalianchiensis FJAT-27215 NCBI: GCA_001685015.1
[Ref.: #69480]	flagellated	yes	82.269	no
[Ref.: #69480]	gram-positive	yes	88.135	yes
[Ref.: #69480]	anaerobic	no	98.615	no
[Ref.: #69480]	aerobic	yes	90.138	yes
[Ref.: #69480]	halophile	no	85.876	yes
[Ref.: #69480]	spore-forming	yes	92.495	no
[Ref.: #69480]	thermophile	no	98.57	no
[Ref.: #69480]	glucose-util	yes	80.081	yes
[Ref.: #69480]	motile	yes	90.995	yes
[Ref.: #69480]	glucose-ferment	no	92.462	no

Availability in culture collections External links

[Ref.: #42961]

Culture collection no.

DSM 100757, CCTCC AB 2015266

[Ref.: #96652]

SI-ID 407339

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Taxonomic insights into the phylogeny of Bacillus badius and proposal for its reclassification to the genus Pseudobacillus as Pseudobacillus badius comb. nov. and reclassification of Bacillus wudalianchiensis Liu et al., 2017 as Pseudobacillus wudalianchiensis comb. nov.	Verma A, Pal Y, Ojha AK, Kumari M, Khatri I, Rameshkumar N, Schumann P, Dastager SG, Mayilraj S, Subramanian S, Krishnamurthi S	Syst Appl Microbiol	10.1016/j.syapm.2019.03.003	2019	*
Phylogeny	Bacillus wudalianchiensis sp. nov., isolated from grass soils of the Wudalianchi scenic area.	Liu B, Liu GH, Sengonca C, Schumann P, Wang JP, Zhu YJ, Zhang HF	Int J Syst Evol Microbiol	10.1099/ijsem.0.002042	2017	*
Phylogeny	Bacillus aerolatus sp. nov., a novel member of the genus Bacillus, isolated from bioaerosols in a school playground.	Chen P, Wang D, Ren Q, Wu J, Jiang Y, Wu Z, Pan Y, Zhong Y, Guan Y, Chen K, Zhang G	Arch Microbiol	10.1007/s00203-020-01955-3	2020	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#42961

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100757

#43454

Bo Liu, Guo-Hong Liu, Cetin Sengonca, Peter Schumann, Jie-Ping Wang, Yu-Jing Zhu, Hai-Feng Zhang: Bacillus wudalianchiensis sp. nov., isolated from grass soils of the Wudalianchi scenic area. IJSEM 67: 2897 - 2902 2017 ( DOI 10.1099/ijsem.0.002042 , PubMed 28820124 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#96652

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID407339.1 )

* These data were automatically processed and therefore are not curated

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