Neobacillus notoginsengisoli (DSM 29196, JCM 30743, SYP-B691)

Name: Neobacillus notoginsengisoli (DSM 29196, JCM 30743, SYP-B691)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 140352

Type strain

Strain Designation SYP-B691

Culture col. no. DSM 29196 JCM 30743 SYP-B691

NCBI tax ID(s) 1578198

Special Collections DSMZ JCM Literature strains

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Neobacillus notoginsengisoli SYP-B691 is an aerobe, spore-forming, Gram-positive prokaryote that was isolated from soil, rhizosphere of Panax notoginseng.

spore-forming Gram-positive motile rod-shaped aerobe genome sequence 16S sequence

Name and taxonomic classification

SI-ID 405070

JCM 30743,DSM 29196

@ref 20215

Last LPSN update 2025-12-16 10:03:34

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Cytobacillaceae

Genus Neobacillus

Species Neobacillus notoginsengisoli

Full scientific name Neobacillus notoginsengisoli (Zhang et al. 2017) Patel and Gupta 2020

Synonyms (1)

Bacillus notoginsengisoli

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility	Flagellum arrangement
43449	positive	1.1-5.9 µm	0.4-0.7 µm	rod-shaped		peritrichous

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
42959	R2A MEDIUM (DSMZ Medium 830)		Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
42959	positive	growth	30	mesophilic
43449	positive	growth	21-42
43449	positive	optimum	37	mesophilic
67770	positive	growth	28	mesophilic

Culture pH

@ref	Ability	Type	PH
43449	positive	optimum	7-9
43449	positive	growth	5-10

Physiology and metabolism

Oxygen tolerance

43449

Oxygen toleranceaerobe

Spore formation

@ref	Spore description	Type of spore	Spore formation
43449	ellipsoidal, located sub-terminally and centrally in swollen sporangia	endospore

Halophily

@ref	Salt	Growth	Tested relation	Concentration
43449	NaCl	positive	growth	0-3 %(w/v)
43449	NaCl	positive	optimum	0-2 %(w/v)

Observation

67770

Observationquinones: MK-7

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43449	64552 ChEBI	2-hydroxybutyrate	-	assimilation
43449	30916 ChEBI	2-oxoglutarate	-	assimilation
43449	73918 ChEBI	3-O-methyl-D-glucose	-	assimilation
43449	13705 ChEBI	acetoacetate	-	assimilation
43449	17925 ChEBI	alpha-D-glucose	+	assimilation
43449		casein	-	hydrolysis
43449	17057 ChEBI	cellobiose	-	assimilation
43449	62968 ChEBI	cellulose	-	hydrolysis
43449	16947 ChEBI	citrate	-	assimilation
43449	18333 ChEBI	D-arabitol	-	assimilation
43449	29990 ChEBI	D-aspartate	-	assimilation
43449	78697 ChEBI	D-fructose 6-phosphate	+	assimilation
43449	28847 ChEBI	D-fucose	-	assimilation
43449	18024 ChEBI	D-galacturonic acid	-	assimilation
43449	8391 ChEBI	D-gluconate	+	assimilation
43449	16899 ChEBI	D-mannitol	-	assimilation
43449	16024 ChEBI	D-mannose	-	assimilation
43449	17924 ChEBI	D-sorbitol	-	assimilation
43449	23652 ChEBI	dextrin	-	assimilation
43449	4853 ChEBI	esculin	+	hydrolysis
43449	15740 ChEBI	formate	-	assimilation
43449	16537 ChEBI	galactarate	-	assimilation
43449	16865 ChEBI	gamma-aminobutyric acid	-	assimilation
43449	5291 ChEBI	gelatin	-	hydrolysis
43449	5291 ChEBI	gelatin	+	assimilation
43449	28066 ChEBI	gentiobiose	-	assimilation
43449	24265 ChEBI	gluconate	+	assimilation
43449	17234 ChEBI	glucose	-	fermentation
43449	70744 ChEBI	glycine-proline	+	assimilation
43449	30849 ChEBI	L-arabinose	+	assimilation
43449	16467 ChEBI	L-arginine	-	assimilation
43449	18287 ChEBI	L-fucose	-	assimilation
43449	15971 ChEBI	L-histidine	-	assimilation
43449	18183 ChEBI	L-pyroglutamic acid	-	assimilation
43449	62345 ChEBI	L-rhamnose	-	assimilation
43449	24996 ChEBI	lactate	+	assimilation
43449	17716 ChEBI	lactose	-	assimilation
43449	25115 ChEBI	malate	-	assimilation
43449	17306 ChEBI	maltose	+	assimilation
43449	28053 ChEBI	melibiose	-	assimilation
43449	17268 ChEBI	myo-inositol	-	assimilation
43449	28037 ChEBI	N-acetylgalactosamine	-	assimilation
43449	506227 ChEBI	N-acetylglucosamine	+	assimilation
43449	17632 ChEBI	nitrate	+	reduction
43449	17309 ChEBI	pectin	-	assimilation
43449	17272 ChEBI	propionate	-	assimilation
43449	26490 ChEBI	quinate	-	assimilation
43449	16634 ChEBI	raffinose	-	assimilation
43449	17814 ChEBI	salicin	-	assimilation
43449	17164 ChEBI	stachyose	-	assimilation
43449	28017 ChEBI	starch	-	hydrolysis
43449	17992 ChEBI	sucrose	-	assimilation
43449	27082 ChEBI	trehalose	+	assimilation
43449	32528 ChEBI	turanose	-	assimilation
43449	53424 ChEBI	tween 20	-	hydrolysis
43449	53423 ChEBI	tween 40	+	hydrolysis
43449	53425 ChEBI	tween 60	+	hydrolysis
43449	53426 ChEBI	tween 80	-	hydrolysis

Metabolite production

@ref	Chebi-ID	Metabolite
43449	15688 ChEBI	acetoin
43449	16136 ChEBI	hydrogen sulfide
43449	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
43449	15688 ChEBI	acetoin	-
43449	17234 ChEBI	glucose

Enzymes

@ref	Value	Activity	Ec
43449	acid phosphatase	+	3.1.3.2
43449	alkaline phosphatase	+	3.1.3.1
43449	arginine dihydrolase	-	3.5.3.6
43449	catalase	+	1.11.1.6
43449	cystine arylamidase	+	3.4.11.3
43449	cytochrome oxidase	-	1.9.3.1
43449	leucine arylamidase	+	3.4.11.1
43449	trypsin	+	3.4.21.4
43449	urease	+	3.5.1.5
43449	valine arylamidase	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Rhizosphere

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude
42959	soil, rhizosphere of Panax notoginseng	Panax notoginseng	Yunnan Province (23° 48' 3.64'' N, 103° 37' 50.04'' E)	China	CHN	Asia	23.801	103.631 23.801/103.631
43449	rhizospheric soil of Panax notoginseng		Yunnan Province, PR China	China	CHN	Asia	23.801	103.631 23.801/103.631
67770	Rhizospheric soil of Panax notoginseng	Panax notoginseng	Yunnan Province	China	CHN	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
42959	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM351568v1 assembly for Neobacillus notoginsengisoli JCM 30743	contig	GCA_003515685	1578198.3	2997752372	1578198	63.72

Genome browser: GCA_003515685

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
42959	Neobacillus notoginsengisoli strain SYP-B691 16S ribosomal RNA gene, partial sequence	KP076294	1530		1578198

GC content

@ref	GC-content (mol%)	Method
42959	45.2	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Neobacillus notoginsengisoli JCM 30743
NCBI: GCA_003515685

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	83.50	no
125439	motility	BacteriaNetⓘ	yes	72.90	no
125439	gram_stain	BacteriaNetⓘ	variable	85.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	54.90	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Bacillus notoginsengisoli strain JCM 30743
PATRIC: 1578198.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	75.73	yes
125438	anaerobic	anaerobicⓘ	no	92.19	yes
125438	spore-forming	spore-formingⓘ	yes	87.96	no
125438	aerobic	aerobicⓘ	yes	69.31	yes
125438	thermophilic	thermophileⓘ	no	88.57	yes
125438	flagellated	motile2+ⓘ	yes	71.00	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Neobacillus driksii sp. nov. isolated from a Mars 2020 spacecraft assembly facility and genomic potential for lasso peptide production in Neobacillus.	Hameed A, McDonagh F, Sengupta P, Miliotis G, Sivabalan SKM, Szydlowski L, Simpson A, Singh NK, Rekha PD, Raman K, Venkateswaran K.	Microbiol Spectr	10.1128/spectrum.01376-24	2025
Phylogeny	Two novel species of the family Bacillaceae: Oceanobacillus piezotolerans sp. nov. and Bacillus piezotolerans sp. nov., from deep-sea sediment samples of Yap Trench.	Yu L, Tang X, Wei S, Qiu Y, Xu X, Xu G, Wang Q, Yang Q	Int J Syst Evol Microbiol	10.1099/ijsem.0.003559	2019
Phylogeny	Bacillus notoginsengisoli sp. nov., a novel bacterium isolated from the rhizosphere of Panax notoginseng.	Zhang MY, Cheng J, Cai Y, Zhang TY, Wu YY, Manikprabhu D, Li WJ, Zhang YX	Int J Syst Evol Microbiol	10.1099/ijsem.0.001975	2017

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#42959	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29196
#43449	Meng-Yue Zhang, Juan Cheng, Ying Cai, Tian-Yuan Zhang, Ying-Ying Wu, Deene Manikprabhu, Wen-Jun Li, Yi-Xuan Zhang: Bacillus notoginsengisoli sp. nov., a novel bacterium isolated from the rhizosphere of Panax notoginseng. IJSEM 67: 2581 - 2585 2017 ( DOI 10.1099/ijsem.0.001975 , PubMed 28786779 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Neobacillus notoginsengisoli (DSM 29196, JCM 30743, SYP-B691)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_003515685

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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