Name: Paraglaciecola hydrolytica S66
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140314

Type strain:

Species: Paraglaciecola hydrolytica

Strain Designation: S66

Culture col. no.: DSM 102834, LMG 29457, NCIMB 15060

Strain history: <- P. Stougaard, Univ. Copenhagen, Frederiksberg, Denmark; S66 <- M. Schultz-Johansen

NCBI tax ID(s): 1799789 (species)

SI-ID 400266

DSM 102834

Section

Paraglaciecola hydrolytica S66 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from eaves of eelgrass .

colony-forming
Gram-negative
motile
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Alteromonadales*
	Family	*Alteromonadaceae*
	Genus	*Paraglaciecola*
	Species	**Paraglaciecola hydrolytica**
	Full Scientific Name (LPSN)	Paraglaciecola hydrolytica Bech et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43329]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.996

[Ref.: #43329]	Cell length	0.8-1 µm

[Ref.: #43329]	Cell width	0.4-0.6 µm

[Ref.: #43329]	Cell shape	rod-shaped

[Ref.: #43329]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %97.733

[Ref.: #43329]	Flagellum arrangement	polar

[Ref.: #43329]	Colony color	beige - pale orange
[Ref.: #43329]	Colony shape	circular
[Ref.: #43329]	Cultivation medium used	Marine agar 2216

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43329]

Culture medium

Marine agar (MA)

[Ref.: #43329]

Culture medium growth

[Ref.: #42921]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #42921]

Culture medium growth

[Ref.: #42921]

Culture medium link

Medium recipe at MediaDive

[Ref.: #42921]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #42921]	growth	28 °C
[Ref.: #43329]	optimum	20-25 °C
[Ref.: #43329]	growth	10-25 °C

[Ref.: #42921]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #43329]			optimum	7.5
[Ref.: #43329]			growth	7-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43329]

Oxygen tolerance

aerobe

[Ref.: #43329]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.987

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43329]		NaCl		growth	1-6	%
[Ref.: #43329]		NaCl		optimum	3-4	%

[Ref.: #43329]

Observation

quinone Q-8

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43329]	agar ChEBI	+	degradation
[Ref.: #43329]	alginate ChEBI	+	degradation
[Ref.: #43329]	carrageenan ChEBI	+	degradation
[Ref.: #43329]	citrate ChEBI	-	carbon source
[Ref.: #43329]	D-glucose ChEBI	+	carbon source
[Ref.: #43329]	D-mannitol ChEBI	+	carbon source
[Ref.: #43329]	D-mannose ChEBI	+/-	carbon source
[Ref.: #43329]	D-xylose ChEBI	+	carbon source
[Ref.: #43329]	decanoate ChEBI	-	carbon source
[Ref.: #43329]	esculin ChEBI	+	degradation
[Ref.: #43329]	galactose ChEBI	+/-	carbon source
[Ref.: #43329]	indole ChEBI	-	reduction
[Ref.: #43329]	L-arabinose ChEBI	+/-	carbon source
[Ref.: #43329]	lactose ChEBI	+	carbon source
[Ref.: #43329]	maltose ChEBI	+	carbon source
[Ref.: #43329]	N-acetylglucosamine ChEBI	-	carbon source
[Ref.: #43329]	nitrate ChEBI	-	reduction
[Ref.: #43329]	phenylacetate ChEBI	-	carbon source
[Ref.: #43329]	starch ChEBI	+	degradation
[Ref.: #43329]	sucrose ChEBI	+	carbon source
[Ref.: #43329]	urea ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #43329]	ampicillin ChEBI	yes	0.5	µg/mL
[Ref.: #43329]	chloramphenicol ChEBI	yes	0.5	µg/mL
[Ref.: #43329]	gentamicin ChEBI	yes	1	µg/mL
[Ref.: #43329]	kanamycin ChEBI	yes	3-4	µg/mL
[Ref.: #43329]	penicillin ChEBI				yes	2	µg/mL
[Ref.: #43329]	streptomycin ChEBI	yes	3-4	µg/mL
[Ref.: #43329]	tetracycline ChEBI	yes	6	µg/mL
[Ref.: #43329]	tobramycin ChEBI	yes	1.5	µg/mL

	Metabolite production	Metabolite	Production
[Ref.: #43329]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43329]	acid phosphatase	+	3.1.3.2
[Ref.: #43329]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43329]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #43329]	arginine dihydrolase	-	3.5.3.6
[Ref.: #43329]	beta-galactosidase	+	3.2.1.23
[Ref.: #43329]	beta-glucosidase	+/-	3.2.1.21
[Ref.: #43329]	catalase	+	1.11.1.6
[Ref.: #43329]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43329]	esterase (C 4)	+
[Ref.: #43329]	esterase Lipase (C 8)	+
[Ref.: #43329]	gelatinase	-
[Ref.: #43329]	leucine arylamidase	+	3.4.11.1
[Ref.: #43329]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #43329]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43329]	trypsin	+/-	3.4.21.4
	Only first 10 entries are displayed. Click here to see all.

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	cyanate degradation	100	3 of 3
[Ref.: #66794]	ribulose monophosphate pathway	100	2 of 2
[Ref.: #66794]	pentose phosphate pathway	100	11 of 11
[Ref.: #66794]	valine metabolism	100	9 of 9
[Ref.: #66794]	Entner Doudoroff pathway	100	10 of 10
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	cis-vaccenate biosynthesis	100	2 of 2
[Ref.: #66794]	ethanol fermentation	100	2 of 2
[Ref.: #66794]	octane oxidation	100	3 of 3
[Ref.: #66794]	biotin biosynthesis	100	4 of 4
[Ref.: #66794]	starch degradation	100	10 of 10
[Ref.: #66794]	palmitate biosynthesis	100	22 of 22
[Ref.: #66794]	formaldehyde oxidation	100	3 of 3
[Ref.: #66794]	gluconeogenesis	100	8 of 8
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	cellulose degradation	100	5 of 5
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	suberin monomers biosynthesis	100	2 of 2
[Ref.: #66794]	photosynthesis	92.86	13 of 14
[Ref.: #66794]	threonine metabolism	90	9 of 10
[Ref.: #66794]	aspartate and asparagine metabolism	88.89	8 of 9
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	lipid A biosynthesis	88.89	8 of 9
[Ref.: #66794]	serine metabolism	88.89	8 of 9
[Ref.: #66794]	C4 and CAM-carbon fixation	87.5	7 of 8
[Ref.: #66794]	isoleucine metabolism	87.5	7 of 8
[Ref.: #66794]	reductive acetyl coenzyme A pathway	85.71	6 of 7
[Ref.: #66794]	tetrahydrofolate metabolism	85.71	12 of 14
[Ref.: #66794]	ubiquinone biosynthesis	85.71	6 of 7
[Ref.: #66794]	glutamate and glutamine metabolism	85.71	24 of 28
[Ref.: #66794]	phenylalanine metabolism	84.62	11 of 13
[Ref.: #66794]	degradation of sugar acids	84	21 of 25
[Ref.: #66794]	glycolate and glyoxylate degradation	83.33	5 of 6
[Ref.: #66794]	NAD metabolism	83.33	15 of 18
[Ref.: #66794]	glycolysis	82.35	14 of 17
[Ref.: #66794]	purine metabolism	80.85	76 of 94
[Ref.: #66794]	gallate degradation	80	4 of 5
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	citric acid cycle	78.57	11 of 14
[Ref.: #66794]	glutathione metabolism	78.57	11 of 14
[Ref.: #66794]	heme metabolism	78.57	11 of 14
[Ref.: #66794]	degradation of hexoses	77.78	14 of 18
[Ref.: #66794]	CO2 fixation in Crenarchaeota	77.78	7 of 9
[Ref.: #66794]	molybdenum cofactor biosynthesis	77.78	7 of 9
[Ref.: #66794]	pyrimidine metabolism	77.78	35 of 45
[Ref.: #66794]	leucine metabolism	76.92	10 of 13
[Ref.: #66794]	degradation of pentoses	75	21 of 28
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	sulfopterin metabolism	75	3 of 4
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
[Ref.: #66794]	CMP-KDO biosynthesis	75	3 of 4
[Ref.: #66794]	tryptophan metabolism	73.68	28 of 38
[Ref.: #66794]	3-phenylpropionate degradation	73.33	11 of 15
[Ref.: #66794]	flavin biosynthesis	73.33	11 of 15
[Ref.: #66794]	proline metabolism	72.73	8 of 11
[Ref.: #66794]	d-xylose degradation	72.73	8 of 11
[Ref.: #66794]	alanine metabolism	72.41	21 of 29
[Ref.: #66794]	cysteine metabolism	72.22	13 of 18
[Ref.: #66794]	cardiolipin biosynthesis	71.43	5 of 7
[Ref.: #66794]	propanol degradation	71.43	5 of 7
[Ref.: #66794]	lipid metabolism	70.97	22 of 31
[Ref.: #66794]	propionate fermentation	70	7 of 10
[Ref.: #66794]	histidine metabolism	68.97	20 of 29
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	66.67	8 of 12
[Ref.: #66794]	arginine metabolism	66.67	16 of 24
[Ref.: #66794]	L-lactaldehyde degradation	66.67	2 of 3
[Ref.: #66794]	d-mannose degradation	66.67	6 of 9
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	methane metabolism	66.67	2 of 3
[Ref.: #66794]	sulfoquinovose degradation	66.67	2 of 3
[Ref.: #66794]	lysine metabolism	64.29	27 of 42
[Ref.: #66794]	vitamin B6 metabolism	63.64	7 of 11
[Ref.: #66794]	dTDPLrhamnose biosynthesis	62.5	5 of 8
[Ref.: #66794]	ketogluconate metabolism	62.5	5 of 8
[Ref.: #66794]	methionine metabolism	61.54	16 of 26
[Ref.: #66794]	urea cycle	61.54	8 of 13
[Ref.: #66794]	isoprenoid biosynthesis	61.54	16 of 26
[Ref.: #66794]	lipoate biosynthesis	60	3 of 5
[Ref.: #66794]	glycogen metabolism	60	3 of 5
[Ref.: #66794]	phenylacetate degradation (aerobic)	60	3 of 5
[Ref.: #66794]	methylglyoxal degradation	60	3 of 5
[Ref.: #66794]	D-cycloserine biosynthesis	60	3 of 5
[Ref.: #66794]	non-pathway related	57.89	22 of 38
[Ref.: #66794]	tyrosine metabolism	57.14	8 of 14
[Ref.: #66794]	allantoin degradation	55.56	5 of 9
[Ref.: #66794]	polyamine pathway	52.17	12 of 23
[Ref.: #66794]	cyclohexanol degradation	50	2 of 4
[Ref.: #66794]	lactate fermentation	50	2 of 4
[Ref.: #66794]	mannosylglycerate biosynthesis	50	1 of 2
[Ref.: #66794]	butanoate fermentation	50	2 of 4
[Ref.: #66794]	glycine metabolism	50	5 of 10
[Ref.: #66794]	kanosamine biosynthesis II	50	1 of 2
[Ref.: #66794]	phenol degradation	50	10 of 20
[Ref.: #66794]	pantothenate biosynthesis	50	3 of 6
[Ref.: #66794]	quinate degradation	50	1 of 2
[Ref.: #66794]	oxidative phosphorylation	46.15	42 of 91
[Ref.: #66794]	ascorbate metabolism	45.45	10 of 22
[Ref.: #66794]	metabolism of disaccharids	45.45	5 of 11
[Ref.: #66794]	4-hydroxymandelate degradation	44.44	4 of 9
[Ref.: #66794]	arachidonic acid metabolism	44.44	8 of 18
[Ref.: #66794]	coenzyme M biosynthesis	40	4 of 10
[Ref.: #66794]	arachidonate biosynthesis	40	2 of 5
[Ref.: #66794]	myo-inositol biosynthesis	40	4 of 10
[Ref.: #66794]	metabolism of amino sugars and derivatives	40	2 of 5
[Ref.: #66794]	vitamin B1 metabolism	38.46	5 of 13
[Ref.: #66794]	degradation of sugar alcohols	37.5	6 of 16
[Ref.: #66794]	androgen and estrogen metabolism	37.5	6 of 16
[Ref.: #66794]	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
[Ref.: #66794]	enterobactin biosynthesis	33.33	1 of 3
[Ref.: #66794]	nitrate assimilation	33.33	3 of 9
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	selenocysteine biosynthesis	33.33	2 of 6
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	phenylpropanoid biosynthesis	30.77	4 of 13
[Ref.: #66794]	sulfate reduction	30.77	4 of 13
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	30.77	4 of 13
[Ref.: #66794]	4-hydroxyphenylacetate degradation	30	3 of 10
[Ref.: #66794]	benzoyl-CoA degradation	28.57	2 of 7
[Ref.: #66794]	chlorophyll metabolism	27.78	5 of 18
[Ref.: #66794]	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
[Ref.: #66794]	carotenoid biosynthesis	27.27	6 of 22
[Ref.: #66794]	toluene degradation	25	1 of 4
[Ref.: #66794]	vitamin E metabolism	25	1 of 4
[Ref.: #66794]	catecholamine biosynthesis	25	1 of 4
[Ref.: #66794]	carnitine metabolism	25	2 of 8
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #42921]	Sample type/isolated from	eaves of eelgrass (Zostera sp.)
[Ref.: #42921]	Host species	Zostera
[Ref.: #42921]	Geographic location (country and/or sea, region)	coast of North Zealand
[Ref.: #42921]	Country	Denmark
[Ref.: #42921]	Country ISO 3 Code	DNK
[Ref.: #42921]	Continent	Europe

[Ref.: #43329]	Sample type/isolated from	leaves from Eelgrass (Zostera sp.) collected on the coast of northwest Zealand, Denmark
[Ref.: #43329]	Geographic location (country and/or sea, region)	northwest Zealand
[Ref.: #43329]	Country	Denmark
[Ref.: #43329]	Country ISO 3 Code	DNK
[Ref.: #43329]	Continent	Europe
[Ref.: #43329]	Enrichment culture	Marine broth 2216
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Aquatic plant
#Host Body-Site	#Plant	#Leaf (Phyllosphere)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KY614189 (>99% sequence identity) for Paraglaciecola hydrolytica subclade from Microbeatlas

Aquatic Samples	521
Soil Samples	36
Animal Samples	812
Plant Samples	34
Total Samples	1403

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #42921]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #42921]	Paraglaciecola hydrolytica strain S66 16S ribosomal RNA gene, partial sequence	KY614189	1535	ENA	1799789 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Paraglaciecola hydrolytica S66	GCA_001565895	scaffold	GenBank	1799789 tax ID	*
[Ref.: #66792]	Paraglaciecola hydrolytica S66	2791354957	draft	JGI IMG/M	1799789 tax ID	*

[Ref.: #43329]	GC-content	42.2 mol%	genome sequence analysis
[Ref.: #42921]	GC-content	42.2 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Paraglaciecola hydrolytica S66 NCBI: GCA_001565895.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Paraglaciecola hydrolytica S66 NCBI: GCA_001565895.1
[Ref.: #69480]	flagellated	yes	87.371	yes
[Ref.: #69480]	gram-positive	no	98.875	yes
[Ref.: #69480]	anaerobic	no	99.062	no
[Ref.: #69480]	aerobic	yes	89.683	yes
[Ref.: #69480]	halophile	no	50.974	no
[Ref.: #69480]	spore-forming	no	97.054	no
[Ref.: #69480]	thermophile	no	99.766	yes
[Ref.: #69480]	glucose-util	yes	93.616	yes
[Ref.: #69480]	motile	yes	85.869	yes
[Ref.: #69480]	glucose-ferment	no	76.668	no

Availability in culture collections External links

[Ref.: #42921]

Culture collection no.

DSM 102834, LMG 29457, NCIMB 15060

[Ref.: #96618]

SI-ID 400266

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Paraglaciecola hydrolytica sp. nov., a bacterium with hydrolytic activity against multiple seaweed-derived polysaccharides.	Bech PK, Schultz-Johansen M, Glaring MA, Barbeyron T, Czjzek M, Stougaard P	Int J Syst Evol Microbiol	10.1099/ijsem.0.001933	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#42921

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 102834

#43329

Pernille Kjersgaard Bech, Mikkel Schultz-Johansen, Mikkel Andreas Glaring, Tristan Barbeyron, Mirjam Czjzek, Peter Stougaard: Paraglaciecola hydrolytica sp. nov., a bacterium with hydrolytic activity against multiple seaweed-derived polysaccharides. IJSEM 67: 2242 - 2247 2017 ( DOI 10.1099/ijsem.0.001933 , PubMed 28671532 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#96618

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID400266.1 )

* These data were automatically processed and therefore are not curated

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