Alkaliphilus namsaraevii (DSM 26418, VKM B-2746, X-07-02)

Name: Alkaliphilus namsaraevii (DSM 26418, VKM B-2746, X-07-02)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 140294

Type strain

Strain Designation X-07-02

Culture col. no. DSM 26418 VKM B-2746 X-07-02

NCBI tax ID(s) 1454184

Special Collections DSMZ Literature strains

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Alkaliphilus namsaraevii X-07-02 is an aerotolerant, chemoorganoheterotroph, spore-forming prokaryote that forms circular colonies and was isolated from steppe soda lake.

spore-forming Gram-positive motile rod-shaped colony-forming aerotolerant chemoorganoheterotroph mesophilic 16S sequence

Name and taxonomic classification

SI-ID 399988

VKM B-2746,DSM 26418

@ref 20215

Last LPSN update 2025-12-16 10:03:33

Domain Bacillati

Phylum Bacillota

Class Clostridia

Order Peptostreptococcales

Family Natronincolaceae

Genus Alkaliphilus

Species Alkaliphilus namsaraevii

Full scientific name Alkaliphilus namsaraevii Zakharyuk et al. 2017

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility	Flagellum arrangement
43228	positive	5.6-8.9 µm	0.3 µm	rod-shaped		monotrichous, polar

Colony morphology

@ref	Colony color	Colony shape	Medium used
43228	dark brown	circular	alkaliphilic growth medium (AGM)

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
43228	alkaliphilic growth medium (AGM)
42901	ALKALIPHILUS NAMSARAEVII MEDIUM (DSMZ Medium 1661)		Medium recipe at MediaDive	Name: ALKALIPHILUS NAMSARAEVII MEDIUM (DSMZ Medium 1661) Composition: NaCl 24.9252 g/l Fe(III) citrate 7.27817 g/l Yeast extract 2.49252 g/l NaHCO3 1.84447 g/l Na2CO3 1.19641 g/l KH2PO4 0.498504 g/l MgCl2 x 6 H2O 0.199402 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l NaOH 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l Pyridoxine hydrochloride 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Folic acid 1.99402e-05 g/l Biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
42901	positive	growth	30	mesophilic
43228	positive	optimum	30	mesophilic
43228	positive	growth	25-47

Culture pH

@ref	Ability	Type	PH	PH range
43228	positive	optimum	9.6-10.3
43228	positive	growth	7.0-10.7	alkaliphile

Physiology and metabolism

Oxygen tolerance

43228

Oxygen toleranceaerotolerant

Nutrition type

43228

Typechemoorganoheterotroph

Spore formation

@ref	Type of spore	Spore formation
43228	endospore

Halophily

@ref	Salt	Growth	Tested relation	Concentration
43228	NaCl	positive	growth	5-150 g/L
43228	NaCl	positive	optimum	40 g/L

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43228	16449 ChEBI	alanine	-	oxidation
43228		amorphous fe(iii) oxyhydroxid	+	electron acceptor
43228	85115 ChEBI	anthraquinone-2,6-disulfonic acid	-	electron acceptor
43228	22599 ChEBI	arabinose	-	oxidation
43228	29016 ChEBI	arginine	-	oxidation
43228		casamino acids	-	oxidation
43228		casein	-	oxidation
43228	17057 ChEBI	cellobiose	-	oxidation
43228	15354 ChEBI	choline	-	oxidation
43228	16947 ChEBI	citrate	-	oxidation
43228	18211 ChEBI	citrulline	-	oxidation
43228	35899 ChEBI	crotonate	-	electron acceptor
43228	18276 ChEBI	dihydrogen	-	oxidation
43228	33403 ChEBI	elemental sulfur	+	electron acceptor
43228	16236 ChEBI	ethanol	-	oxidation
43228	31605 ChEBI	ferric citrate	+	electron acceptor
43228	28757 ChEBI	fructose	-	oxidation
43228	33984 ChEBI	fucose	-	oxidation
43228	29806 ChEBI	fumarate	-	electron acceptor
43228	28260 ChEBI	galactose	-	oxidation
43228	17234 ChEBI	glucose	-	oxidation
43228	28300 ChEBI	glutamine	-	oxidation
43228	27570 ChEBI	histidine	-	oxidation
43228	24996 ChEBI	lactate	-	oxidation
43228	17716 ChEBI	lactose	-	oxidation
43228	25094 ChEBI	lysine	-	oxidation
43228	17306 ChEBI	maltose	-	oxidation
43228		manganese dioxide	-	electron acceptor
43228	37684 ChEBI	mannose	-	oxidation
43228	28053 ChEBI	melibiose	-	oxidation
43228	17790 ChEBI	methanol	-	oxidation
43228	17268 ChEBI	myo-inositol	-	oxidation
43228	17632 ChEBI	nitrate	-	electron acceptor
43228	16301 ChEBI	nitrite	-	electron acceptor
43228	18257 ChEBI	ornithine	-	oxidation
43228		peptone	+	oxidation
43228	26271 ChEBI	proline	-	oxidation
43228	16634 ChEBI	raffinose	-	oxidation
43228	26546 ChEBI	rhamnose	-	oxidation
43228	33942 ChEBI	ribose	-	oxidation
43228	17822 ChEBI	serine	-	oxidation
43228	32954 ChEBI	sodium acetate	+	electron donor
43228	30911 ChEBI	sorbitol	-	oxidation
43228	27922 ChEBI	sorbose	-	oxidation
43228	17992 ChEBI	sucrose	-	oxidation
43228	16189 ChEBI	sulfate	-	electron acceptor
43228	17359 ChEBI	sulfite	-	electron acceptor
43228	16094 ChEBI	thiosulfate	-	electron acceptor
43228	27082 ChEBI	trehalose	-	oxidation
43228		tryptone	+	oxidation
43228	27266 ChEBI	valine	-	oxidation
43228	18222 ChEBI	xylose	-	oxidation
43228		yeast extract	+	oxidation

Antibiotic resistance

@ref	ChEBI	Metabolite	Sensitivity conc.	Resistance conc.
43228	28971	ampicillin	1000 µg/mL
43228	28669	bacitracin	250 µg/mL
43228	204928	cefotaxime		100 µg/mL
43228	6104	kanamycin	250 µg/mL
43228	18208	penicillin g	1000 µg/mL
43228	28077	rifampicin	25000 µg/mL
43228	17076	streptomycin	1000 µg/mL
43228	28001	vancomycin		250 µg/mL

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Lake (large)
#Environmental	#Aquatic	#Non-marine Saline and Alkaline

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude	Enrichment culture	Enrichment culture composition	Enrichment culture duration	Enrichment culture temperature	Isolation procedure
42901	steppe soda lake	Transbaikal region, Buryatia, Lake Khilganta	Russia	RUS	Asia	50.7149	115.102 50.7149/115.102
43228	sediments of alkaline lake from 0.4 m depth	Lake Khilganta, South Siberian steppe	Russia	RUS	Asia	50	115 50/115	alkaliphilic growth medium (AGM)	1.2 g/l Na2CO3, 1.85 g/l NaHCO3, 15 g/l NaCl, 0.5 g/l K2HPO4, 0.1 g/l MgCl2x6H2O, 0.5 g/l NH4Cl, 0.025 g/l yeast extract (Difco), 10 ml trace element solution SL-10 (medium 320; DSMZ), pH 9.6	14 days	30	serial tenfold dilution, Hungate roll tube technique

Taxonmaps

69479

Global distribution of 16S sequence KJ196388 (>99% sequence identity) for Alkaliphilus from Microbeatlas

Aquatic Samples	16
Soil Samples	3
Animal Samples
Plant Samples
Total Samples	19

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
42901	1	Risk group (German classification)

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
42901	Alkaliphilus namsaraevii strain X-07-2 16S ribosomal RNA gene, partial sequence	KJ196388	1474		1454184

GC content

@ref	GC-content (mol%)	Method
42901	32.6	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Literature

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#42901	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 26418
#43228	Anastasiya Zakharyuk, Lyudmila Kozyreva, Elena Ariskina, Olga Troshina, Dmitry Kopitsyn, Viktoria Shcherbakova: Alkaliphilus namsaraevii sp. nov., an alkaliphilic iron- and sulfur-reducing bacterium isolated from a steppe soda lake. IJSEM 67: 1990 - 1995 2017 ( DOI 10.1099/ijsem.0.001904 , PubMed 28632119 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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