Name: Alkaliphilus namsaraevii X-07-02
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140294

Type strain:

Species: Alkaliphilus namsaraevii

Strain Designation: X-07-02

Culture col. no.: DSM 26418, VKM B-2746

Strain history: <- A. Zakharyuk, Inst. Biochem. Physiol. Microorg., RAS, Pushchino, Russia; X-07-02 <- A. Zakharyuk, Inst. Marine Biol. FEB RAS, Vladivostok {2008}

NCBI tax ID(s): 1454184 (species)

SI-ID 399988

DSM 26418

Section

Alkaliphilus namsaraevii X-07-02 is an aerotolerant, chemoorganoheterotroph, spore-forming bacterium that forms circular colonies and was isolated from steppe soda lake.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
aerotolerant
chemoorganoheterotroph
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Clostridiaceae*
	Genus	*Alkaliphilus*
	Species	**Alkaliphilus namsaraevii**
	Full Scientific Name (LPSN)	Alkaliphilus namsaraevii Zakharyuk et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43228]	Gram stain	positive

[Ref.: #43228]	Cell length	5.6-8.9 µm

[Ref.: #43228]	Cell width	0.3 µm

[Ref.: #43228]	Cell shape	rod-shaped

[Ref.: #43228]	Motility	yes

[Ref.: #43228]	Flagellum arrangement	monotrichous, polar

[Ref.: #43228]	Colony color	dark brown
[Ref.: #43228]	Colony shape	circular
[Ref.: #43228]	Cultivation medium used	alkaliphilic growth medium (AGM)

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43228]

Culture medium

alkaliphilic growth medium (AGM)

[Ref.: #43228]

Culture medium growth

[Ref.: #42901]

Culture medium

ALKALIPHILUS NAMSARAEVII MEDIUM (DSMZ Medium 1661)

[Ref.: #42901]

Culture medium growth

[Ref.: #42901]

Culture medium link

Medium recipe at MediaDive

[Ref.: #42901]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #42901]	growth	30 °C
[Ref.: #43228]	optimum	30 °C
[Ref.: #43228]	growth	25-47 °C

[Ref.: #42901]	Temperature range	mesophilic
[Ref.: #43228]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43228]			optimum	9.6-10.3
[Ref.: #43228]			growth	7.0-10.7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43228]

Oxygen tolerance

aerotolerant

[Ref.: #43228]

Nutrition type

chemoorganoheterotroph

[Ref.: #43228]	Type of spore	endospore
[Ref.: #43228]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43228]		NaCl		growth	5-150	g/L
[Ref.: #43228]		NaCl		optimum	40	g/L

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43228]	alanine ChEBI	-	oxidation
[Ref.: #43228]	amorphous fe(iii) oxyhydroxid	+	electron acceptor
[Ref.: #43228]	anthraquinone-2,6-disulfonic acid ChEBI	-	electron acceptor
[Ref.: #43228]	arabinose ChEBI	-	oxidation
[Ref.: #43228]	arginine ChEBI	-	oxidation
[Ref.: #43228]	casamino acids	-	oxidation
[Ref.: #43228]	casein	-	oxidation
[Ref.: #43228]	cellobiose ChEBI	-	oxidation
[Ref.: #43228]	choline ChEBI	-	oxidation
[Ref.: #43228]	citrate ChEBI	-	oxidation
[Ref.: #43228]	citrulline ChEBI	-	oxidation
[Ref.: #43228]	crotonate ChEBI	-	electron acceptor
[Ref.: #43228]	dihydrogen ChEBI	-	oxidation
[Ref.: #43228]	elemental sulfur ChEBI	+	electron acceptor
[Ref.: #43228]	ethanol ChEBI	-	oxidation
[Ref.: #43228]	ferric citrate ChEBI	+	electron acceptor
[Ref.: #43228]	fructose ChEBI	-	oxidation
[Ref.: #43228]	fucose ChEBI	-	oxidation
[Ref.: #43228]	fumarate ChEBI	-	electron acceptor
[Ref.: #43228]	galactose ChEBI	-	oxidation
[Ref.: #43228]	glucose ChEBI	-	oxidation
[Ref.: #43228]	glutamine ChEBI	-	oxidation
[Ref.: #43228]	histidine ChEBI	-	oxidation
[Ref.: #43228]	lactate ChEBI	-	oxidation
[Ref.: #43228]	lactose ChEBI	-	oxidation
[Ref.: #43228]	lysine ChEBI	-	oxidation
[Ref.: #43228]	maltose ChEBI	-	oxidation
[Ref.: #43228]	manganese dioxide	-	electron acceptor
[Ref.: #43228]	mannose ChEBI	-	oxidation
[Ref.: #43228]	melibiose ChEBI	-	oxidation
[Ref.: #43228]	methanol ChEBI	-	oxidation
[Ref.: #43228]	myo-inositol ChEBI	-	oxidation
[Ref.: #43228]	nitrate ChEBI	-	electron acceptor
[Ref.: #43228]	nitrite ChEBI	-	electron acceptor
[Ref.: #43228]	ornithine ChEBI	-	oxidation
[Ref.: #43228]	peptone	+	oxidation
[Ref.: #43228]	proline ChEBI	-	oxidation
[Ref.: #43228]	raffinose ChEBI	-	oxidation
[Ref.: #43228]	rhamnose ChEBI	-	oxidation
[Ref.: #43228]	ribose ChEBI	-	oxidation
[Ref.: #43228]	serine ChEBI	-	oxidation
[Ref.: #43228]	sodium acetate ChEBI	+	electron donor
[Ref.: #43228]	sorbitol ChEBI	-	oxidation
[Ref.: #43228]	sorbose ChEBI	-	oxidation
[Ref.: #43228]	sucrose ChEBI	-	oxidation
[Ref.: #43228]	sulfate ChEBI	-	electron acceptor
[Ref.: #43228]	sulfite ChEBI	-	electron acceptor
[Ref.: #43228]	thiosulfate ChEBI	-	electron acceptor
[Ref.: #43228]	trehalose ChEBI	-	oxidation
[Ref.: #43228]	tryptone	+	oxidation
[Ref.: #43228]	valine ChEBI	-	oxidation
[Ref.: #43228]	xylose ChEBI	-	oxidation
[Ref.: #43228]	yeast extract	+	oxidation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #43228]	ampicillin ChEBI	yes	1000	µg/mL
[Ref.: #43228]	bacitracin ChEBI	yes	250	µg/mL
[Ref.: #43228]	cefotaxime ChEBI				yes	100	µg/mL
[Ref.: #43228]	kanamycin ChEBI	yes	250	µg/mL
[Ref.: #43228]	penicillin g ChEBI	yes	1000	µg/mL
[Ref.: #43228]	rifampicin ChEBI	yes	25000	µg/mL
[Ref.: #43228]	streptomycin ChEBI	yes	1000	µg/mL
[Ref.: #43228]	vancomycin ChEBI				yes	250	µg/mL

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #42901]	Sample type/isolated from	steppe soda lake
[Ref.: #42901]	Geographic location (country and/or sea, region)	Transbaikal region, Buryatia, Lake Khilganta
[Ref.: #42901]	Country	Russia
[Ref.: #42901]	Country ISO 3 Code	RUS
[Ref.: #42901]	Continent	Asia
[Ref.: #42901]	Geographic location	50.7149°/115.1020°

[Ref.: #43228]	Sample type/isolated from	sediments of alkaline lake from 0.4 m depth
[Ref.: #43228]	Geographic location (country and/or sea, region)	Lake Khilganta, South Siberian steppe
[Ref.: #43228]	Country	Russia
[Ref.: #43228]	Country ISO 3 Code	RUS
[Ref.: #43228]	Continent	Asia
[Ref.: #43228]	Geographic location	50.0000°/115.0000°
[Ref.: #43228]	Enrichment culture	alkaliphilic growth medium (AGM)
[Ref.: #43228]	Enrichment culture composition	1.2 g/l Na2CO3, 1.85 g/l NaHCO3, 15 g/l NaCl, 0.5 g/l K2HPO4, 0.1 g/l MgCl2x6H2O, 0.5 g/l NH4Cl, 0.025 g/l yeast extract (Difco), 10 ml trace element solution SL-10 (medium 320; DSMZ), pH 9.6
[Ref.: #43228]	Enrichment culture duration	14 days
[Ref.: #43228]	Enrichment culture temperature	30 ̊C
[Ref.: #43228]	Isolation/enrichment procedure	serial tenfold dilution, Hungate roll tube technique
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Lake (large)
#Environmental	#Aquatic	#Non-marine Saline and Alkaline

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KJ196388 (>99% sequence identity) for Alkaliphilus from Microbeatlas

Aquatic Samples	16
Soil Samples	3
Animal Samples
Plant Samples
Total Samples	19

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #42901]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #42901]	Alkaliphilus namsaraevii strain X-07-2 16S ribosomal RNA gene, partial sequence	KJ196388	1474	ENA	1454184 tax ID

[Ref.: #42901]

GC-content

32.6 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #42901]

Culture collection no.

DSM 26418, VKM B-2746

[Ref.: #96599]

SI-ID 399988

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#42901

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 26418

#43228

Anastasiya Zakharyuk, Lyudmila Kozyreva, Elena Ariskina, Olga Troshina, Dmitry Kopitsyn, Viktoria Shcherbakova: Alkaliphilus namsaraevii sp. nov., an alkaliphilic iron- and sulfur-reducing bacterium isolated from a steppe soda lake. IJSEM 67: 1990 - 1995 2017 ( DOI 10.1099/ijsem.0.001904 , PubMed 28632119 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#96599

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID399988.1 )

* These data were automatically processed and therefore are not curated

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