Name: Halomonas alkalicola 56-L4-10aEn
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140276

Type strain:

Species: Halomonas alkalicola

Strain Designation: 56-L4-10aEn

Culture col. no.: DSM 103354, CICC 11012s

Strain history: <- Y. Ge, CICC; 56-L4-10aEn

NCBI tax ID(s): 1930622 (species)

SI-ID 403902

DSM 103354

Section

Halomonas alkalicola 56-L4-10aEn is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from soap-making tank of a household product plant.

colony-forming
Gram-negative
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Oceanospirillales*
	Family	*Halomonadaceae*
	Genus	*Halomonas*
	Species	**Halomonas alkalicola**
	Full Scientific Name (LPSN)	Halomonas alkalicola Tang et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43185]	Gram stain	negative

[Ref.: #43185]	Cell length	1.5-2.1 µm

[Ref.: #43185]	Cell width	0.5-0.6 µm

[Ref.: #43185]	Cell shape	rod-shaped

[Ref.: #43185]	Motility	no

[Ref.: #43185]	Colony color	ochre
[Ref.: #43185]	Colony shape	circular
[Ref.: #43185]	Cultivation medium used	tryptic soy agar (TSA)

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43185]

Culture medium

tryptic soy agar (TSA)

[Ref.: #43185]

Culture medium growth

[Ref.: #42883]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #42883]

Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #42883]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #42883]	growth	28 °C
[Ref.: #43185]	optimum	28-30 °C
[Ref.: #43185]	growth	15-37 °C

[Ref.: #42883]	Temperature range	mesophilic
[Ref.: #43185]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43185]			optimum	9-10
[Ref.: #43185]			growth	7-12.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43185]

Oxygen tolerance

aerobe

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Ability of spore formation

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Halophily / tolerance level

moderately halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43185]		NaCl		growth	0-8	%(w/v)
[Ref.: #43185]		NaCl		optimum	0-2	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43185]	2-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #43185]	5-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #43185]	adipate ChEBI	-	assimilation
[Ref.: #43185]	amygdalin ChEBI	-	builds acid from
[Ref.: #43185]	arabinose ChEBI	-	assimilation
[Ref.: #43185]	arbutin ChEBI	-	builds acid from
[Ref.: #43185]	cellobiose ChEBI	-	builds acid from
[Ref.: #43185]	citrate ChEBI	-	carbon source
[Ref.: #43185]	D-arabinose ChEBI	-	builds acid from
[Ref.: #43185]	D-arabitol ChEBI	-	builds acid from
[Ref.: #43185]	D-fructose ChEBI	-	builds acid from
[Ref.: #43185]	D-fucose ChEBI	-	builds acid from
[Ref.: #43185]	D-galactose ChEBI	-	builds acid from
[Ref.: #43185]	D-glucose ChEBI	-	builds acid from
[Ref.: #43185]	D-lyxose ChEBI	-	builds acid from
[Ref.: #43185]	D-mannose ChEBI	-	builds acid from
[Ref.: #43185]	D-ribose ChEBI	-	builds acid from
[Ref.: #43185]	D-tagatose ChEBI	-	builds acid from
[Ref.: #43185]	D-xylose ChEBI	-	builds acid from
[Ref.: #43185]	decanoate ChEBI	-	assimilation
[Ref.: #43185]	dna ChEBI	-	hydrolysis
[Ref.: #43185]	erythritol ChEBI	-	builds acid from
[Ref.: #43185]	esculin ChEBI	-	hydrolysis
[Ref.: #43185]	galactitol ChEBI	-	builds acid from
[Ref.: #43185]	gelatin ChEBI	-	hydrolysis
[Ref.: #43185]	gentiobiose ChEBI	-	builds acid from
[Ref.: #43185]	gluconate ChEBI	-	builds acid from
[Ref.: #43185]	gluconate ChEBI	-	assimilation
[Ref.: #43185]	glucose ChEBI	-	assimilation
[Ref.: #43185]	glycerol ChEBI	-	builds acid from
[Ref.: #43185]	glycogen ChEBI	-	builds acid from
[Ref.: #43185]	inulin ChEBI	-	builds acid from
[Ref.: #43185]	L-arabinose ChEBI	-	builds acid from
[Ref.: #43185]	L-arabitol ChEBI	-	builds acid from
[Ref.: #43185]	L-fucose ChEBI	-	builds acid from
[Ref.: #43185]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #43185]	L-sorbose ChEBI	-	builds acid from
[Ref.: #43185]	L-xylose ChEBI	-	builds acid from
[Ref.: #43185]	lactose ChEBI	-	builds acid from
[Ref.: #43185]	malate ChEBI	-	assimilation
[Ref.: #43185]	maltose ChEBI	-	builds acid from
[Ref.: #43185]	maltose ChEBI	-	assimilation
[Ref.: #43185]	mannitol ChEBI	-	builds acid from
[Ref.: #43185]	mannitol ChEBI	-	assimilation
[Ref.: #43185]	mannose ChEBI	-	assimilation
[Ref.: #43185]	melezitose ChEBI	-	builds acid from
[Ref.: #43185]	melibiose ChEBI	-	builds acid from
[Ref.: #43185]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #43185]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #43185]	myo-inositol ChEBI	-	builds acid from
[Ref.: #43185]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #43185]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #43185]	nitrate ChEBI	-	reduction
[Ref.: #43185]	phenylacetate ChEBI	-	assimilation
[Ref.: #43185]	raffinose ChEBI	+	builds acid from
[Ref.: #43185]	ribitol ChEBI	-	builds acid from
[Ref.: #43185]	salicin ChEBI	-	builds acid from
[Ref.: #43185]	sodium citrate ChEBI	-	assimilation
[Ref.: #43185]	sorbitol ChEBI	-	builds acid from
[Ref.: #43185]	starch ChEBI	-	builds acid from
[Ref.: #43185]	starch ChEBI	-	hydrolysis
[Ref.: #43185]	sucrose ChEBI	-	builds acid from
[Ref.: #43185]	trehalose ChEBI	-	builds acid from
[Ref.: #43185]	turanose ChEBI	-	builds acid from
[Ref.: #43185]	tween 20 ChEBI	-	hydrolysis
[Ref.: #43185]	tween 80 ChEBI	-	hydrolysis
[Ref.: #43185]	tyrosine ChEBI	-	hydrolysis
[Ref.: #43185]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #43185]	acetoin ChEBI	no
[Ref.: #43185]	hydrogen sulfide ChEBI	no
[Ref.: #43185]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Citrate test
[Ref.: #43185]		acetoin ChEBI	-
[Ref.: #43185]		citrate ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #43185]	arginine dihydrolase	-	3.5.3.6
[Ref.: #43185]	beta-galactosidase	-	3.2.1.23
[Ref.: #43185]	catalase	+	1.11.1.6
[Ref.: #43185]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43185]	gelatinase	-
[Ref.: #43185]	lysine decarboxylase	-	4.1.1.18
[Ref.: #43185]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #43185]	tryptophan deaminase	-	4.1.99.1
[Ref.: #43185]	urease	-	3.5.1.5

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43185]

Fatty acid	Percentage
C18:1ω7c / C18:1ω6c	53.9
C16:0	15.2

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #42883]	Sample type/isolated from	soap-making tank of a household product plant
[Ref.: #42883]	Geographic location (country and/or sea, region)	Beijing
[Ref.: #42883]	Country	China
[Ref.: #42883]	Country ISO 3 Code	CHN
[Ref.: #42883]	Continent	Asia

[Ref.: #43185]	Sample type/isolated from	soap-making tank of a household product plant
[Ref.: #43185]	Country	China
[Ref.: #43185]	Country ISO 3 Code	CHN
[Ref.: #43185]	Continent	Asia
[Ref.: #43185]	Enrichment culture	trypticase soy agar (TSA, BD 236950)
[Ref.: #43185]	Isolation/enrichment procedure	serial dilution plating technique
* marker position based on {}

Isolation sources categories

#Engineered	#Built environment	#Container (Reservoir)
#Engineered	#Industrial	#Plant (Factory)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KU530128 (>99% sequence identity) for Halomonas from Microbeatlas

Aquatic Samples	759
Soil Samples	292
Animal Samples	1357
Plant Samples	136
Total Samples	2544

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #42883]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #42883]	Halomonas alkalicola strain CICC 11012s 16S ribosomal RNA gene, partial sequence	KU530128	1500	ENA	1930622 tax ID

[Ref.: #42883]

GC-content

63.2 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #42883]

Culture collection no.

DSM 103354, CICC 11012s

[Ref.: #96582]

SI-ID 403902

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Halomonas alkalicola sp. nov., isolated from a household product plant.	Tang X, Zhai L, Lin Y, Yao S, Wang L, Ge Y, Liu Y, Zhang X, Zhang T, Zhang L, Liu J, Cheng C	Int J Syst Evol Microbiol	10.1099/ijsem.0.001757	2017	*
Metabolism	Mechanism of TonB-dependent transport system in Halomonas alkalicola CICC 11012s in response to alkaline stress.	Zhai L, Xie J, Feng H, Sun S, Cheng K, Yao S	Extremophiles	10.1007/s00792-020-01209-6	2020	*
Phylogeny	Halomonas tibetensis sp. nov., isolated from saline lakes on Tibetan Plateau.	Lu HB, Xing P, Zhai L, Phurbu D, Tang Q, Wu QL	J Microbiol	10.1007/s12275-018-8076-5	2018	*
Genetics	Genome sequencing and heterologous expression of antiporters reveal alkaline response mechanisms of Halomonas alkalicola.	Zhai L, Xie J, Lin Y, Cheng K, Wang L, Yue F, Guo J, Liu J, Yao S	Extremophiles	10.1007/s00792-017-0991-6	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#42883

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 103354

#43185

Xiaoli Tang, Lei Zhai, Yafang Lin, Su Yao, Lijiang Wang, Yuanyuan Ge, Yang Liu, Xin Zhang, Tianci Zhang, Lu Zhang, Jiquan Liu, Chi Cheng: Halomonas alkalicola sp. nov., isolated from a household product plant. IJSEM 67: 1546 - 1550 2017 ( DOI 10.1099/ijsem.0.001757 , PubMed 28005522 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#96582

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403902.1 )

* These data were automatically processed and therefore are not curated

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