Allofournierella massiliensis (DSM 100451, CSUR P2014, AT2)

Name: Allofournierella massiliensis (DSM 100451, CSUR P2014, AT2)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 140267

Type strain

Strain Designation AT2

Culture col. no. DSM 100451 CSUR P2014 AT2

NCBI tax ID(s) 1650663

Special Collections DSMZ Literature strains

History DSMZ <- S. Khelaifia, URMITE, Univ. Méditerranée, Marseille, France; AT2

Links

Allofournierella massiliensis AT2 is an anaerobe, Gram-negative, rod-shaped bacterium that was isolated from human stool specimen, healthy 28-year-old French man.

Gram-negative rod-shaped anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 403978

DSM 100451

@ref 20215

Last LPSN update 2026-05-29 08:39:40

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Eubacteriales

Family Oscillospiraceae

Genus Allofournierella

Species Allofournierella massiliensis

Full scientific name Allofournierella massiliensis (Togo et al. 2017) Oren and Molinari Novoa 2024

Synonyms (1)

Fournierella massiliensis

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
43184	negative	2 µm	0.5 µm	rod-shaped

Colony morphology

@ref	Colony size	Colony color	Medium used
43184	0.3-1 mm	white	5 % sheep blood-enriched Columbia agar

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
43184	5 % sheep blood-enriched Columbia agar
42874	WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339)		Medium recipe at MediaDive	Name: WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339) Composition: dehydrated Wilkins-Chalgren medium 33.0 g/l L-Cysteine HCl 0.3 g/l Sodium resazurin 0.0005 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
42874	positive	growth	37
43184	negative	growth	55
43184	positive	growth	45
43184	positive	optimum	37
43184	positive	growth	28

Culture pH

@ref	Ability	Type	PH	PH range
43184	positive	optimum	7.0-7.3
43184	positive	growth	6-8.5	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
43184	anaerobe
125439	obligate anaerobe	98.933

Spore formation

43184

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
43184	NaCl		growth	10 g/L

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43184	27613 ChEBI	amygdalin	-	builds acid from
43184	18305 ChEBI	arbutin	-	builds acid from
68380	29016 ChEBI	arginine	-	hydrolysis	from API rID32A
43184	17057 ChEBI	cellobiose	-	builds acid from
43184	62968 ChEBI	cellulose	-	assimilation
43184	17108 ChEBI	D-arabinose	-	builds acid from
43184	18333 ChEBI	D-arabitol	-	builds acid from
43184	15824 ChEBI	D-fructose	+	builds acid from
43184	28847 ChEBI	D-fucose	-	builds acid from
43184	12936 ChEBI	D-galactose	+	builds acid from
43184	17634 ChEBI	D-glucose	+	builds acid from
43184	17634 ChEBI	D-glucose	+	assimilation
43184	16899 ChEBI	D-mannitol	-	builds acid from
43184	16899 ChEBI	D-mannitol	-	assimilation
43184	16024 ChEBI	D-mannose	+	builds acid from
43184	16024 ChEBI	D-mannose	+	assimilation
68380	16024 ChEBI	D-mannose	-	fermentation	from API rID32A
43184	16988 ChEBI	D-ribose	-	builds acid from
43184	17924 ChEBI	D-sorbitol	-	builds acid from
43184	17924 ChEBI	D-sorbitol	-	assimilation
43184	16443 ChEBI	D-tagatose	-	builds acid from
43184	65327 ChEBI	D-xylose	-	builds acid from
43184	65327 ChEBI	D-xylose	-	assimilation
43184	17113 ChEBI	erythritol	-	builds acid from
43184		esculin ferric citrate	+	builds acid from
43184		esculin ferric citrate	+	assimilation
43184	16813 ChEBI	galactitol	-	builds acid from
43184	5291 ChEBI	gelatin	-	hydrolysis
43184	28066 ChEBI	gentiobiose	-	builds acid from
43184	17754 ChEBI	glycerol	+	builds acid from
43184	17754 ChEBI	glycerol	+	assimilation
43184	28087 ChEBI	glycogen	-	builds acid from
43184	15443 ChEBI	inulin	-	builds acid from
43184	30849 ChEBI	L-arabinose	-	builds acid from
43184	30849 ChEBI	L-arabinose	-	assimilation
43184	18403 ChEBI	L-arabitol	-	builds acid from
43184	18287 ChEBI	L-fucose	-	builds acid from
68380	29985 ChEBI	L-glutamate	-	degradation	from API rID32A
43184	62345 ChEBI	L-rhamnose	-	builds acid from
43184	62345 ChEBI	L-rhamnose	-	assimilation
43184	17266 ChEBI	L-sorbose	-	builds acid from
43184	65328 ChEBI	L-xylose	-	builds acid from
43184	17716 ChEBI	lactose	+	builds acid from
43184	17716 ChEBI	lactose	+	assimilation
43184	17306 ChEBI	maltose	+	builds acid from
43184	17306 ChEBI	maltose	+	assimilation
43184	6731 ChEBI	melezitose	+	builds acid from
43184	6731 ChEBI	melezitose	+	assimilation
43184	28053 ChEBI	melibiose	+	builds acid from
43184		methyl alpha-D-glucopyranoside	+	builds acid from
43184	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from
43184	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from
43184	17268 ChEBI	myo-inositol	-	builds acid from
43184	506227 ChEBI	N-acetylglucosamine	-	builds acid from
68380	17632 ChEBI	nitrate	-	reduction	from API rID32A
43184		potassium 2-dehydro-D-gluconate	-	builds acid from
43184		potassium 5-dehydro-D-gluconate	+	builds acid from
43184	32032 ChEBI	potassium gluconate	-	builds acid from
43184	16634 ChEBI	raffinose	+	builds acid from
43184	16634 ChEBI	raffinose	+	assimilation
68380	16634 ChEBI	raffinose	-	fermentation	from API rID32A
43184	15963 ChEBI	ribitol	-	builds acid from
43184	17814 ChEBI	salicin	+	builds acid from
43184	17814 ChEBI	salicin	+	assimilation
43184	28017 ChEBI	starch	-	builds acid from
43184	17992 ChEBI	sucrose	+	builds acid from
43184	17992 ChEBI	sucrose	+	assimilation
43184	27082 ChEBI	trehalose	-	builds acid from
43184	27082 ChEBI	trehalose	-	assimilation
68380	27897 ChEBI	tryptophan	-	energy source	from API rID32A
43184	32528 ChEBI	turanose	+	builds acid from
68380	16199 ChEBI	urea	-	hydrolysis	from API rID32A
43184	17151 ChEBI	xylitol	-	builds acid from

Metabolite production

@ref	Chebi-ID	Metabolite	Production
43184	35581 ChEBI	indole
68380	35581 ChEBI	indole		from API rID32A

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68380	35581 ChEBI	indole	-	from API rID32A

Enzymes

@ref	Value	Activity	Ec
43184	acid phosphatase	+	3.1.3.2
68380	alanine arylamidase	-	3.4.11.2	from API rID32A
43184	alkaline phosphatase	-	3.1.3.1
68380	alkaline phosphatase	-	3.1.3.1	from API rID32A
68380	alpha-arabinosidase	-	3.2.1.55	from API rID32A
43184	alpha-chymotrypsin	-	3.4.21.1
43184	alpha-fucosidase	-	3.2.1.51
68380	alpha-fucosidase	-	3.2.1.51	from API rID32A
43184	alpha-galactosidase	+	3.2.1.22
68380	alpha-galactosidase	+	3.2.1.22	from API rID32A
43184	alpha-glucosidase	+	3.2.1.20
68380	alpha-glucosidase	+	3.2.1.20	from API rID32A
43184	alpha-mannosidase	-	3.2.1.24
68380	arginine dihydrolase	-	3.5.3.6	from API rID32A
43184	beta-galactosidase	-	3.2.1.23
68380	beta-galactosidase	+	3.2.1.23	from API rID32A
68380	beta-Galactosidase 6-phosphate	-		from API rID32A
43184	beta-glucosidase	+	3.2.1.21
68380	beta-glucosidase	+	3.2.1.21	from API rID32A
43184	beta-glucuronidase	-	3.2.1.31
68380	beta-glucuronidase	-	3.2.1.31	from API rID32A
43184	cystine arylamidase	-	3.4.11.3
43184	esterase (C 4)	+
43184	esterase Lipase (C 8)	+
68380	glutamate decarboxylase	-	4.1.1.15	from API rID32A
68380	glutamyl-glutamate arylamidase	-		from API rID32A
68380	glycin arylamidase	-		from API rID32A
68380	histidine arylamidase	-		from API rID32A
68380	L-arginine arylamidase	-		from API rID32A
43184	leucine arylamidase	-	3.4.11.1
68380	leucine arylamidase	-	3.4.11.1	from API rID32A
68380	leucyl glycin arylamidase	-	3.4.11.1	from API rID32A
43184	lipase	-
43184	N-acetyl-beta-glucosaminidase	+	3.2.1.52
68380	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API rID32A
43184	naphthol-AS-BI-phosphohydrolase	+
68380	phenylalanine arylamidase	-		from API rID32A
68380	proline-arylamidase	-	3.4.11.5	from API rID32A
68380	pyrrolidonyl arylamidase	-	3.4.19.3	from API rID32A
68380	serine arylamidase	-		from API rID32A
43184	trypsin	-	3.4.21.4
68380	tryptophan deaminase	-	4.1.99.1	from API rID32A
68380	tyrosine arylamidase	-		from API rID32A
43184	urease	-	3.5.1.5
68380	urease	-	3.5.1.5	from API rID32A
43184	valine arylamidase	-

API rID32A

@ref	URE	ADH (Arg)	alpha GAL	beta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha GLU	beta GLU	alpha ARA	beta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	MNE	RAF	GDC	alpha FUC	Reduction of nitrateNIT	IND	PAL	L-arginine arylamidaseArgA	ProA	LGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	PyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
42874	-	-	+	+	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Infection	#Patient	#Specimen
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)
#Host	#Human	#Male

Isolation

@ref	Sample type	Host species	Country	Country ISO 3 Code	Continent	Enrichment culture	Enrichment culture duration	Enrichment culture temperature
42874	human stool specimen, healthy 28-year-old French man	Homo sapiens	France	FRA	Europe
43184	fresh stool sample from a healthy 28-year-old French man	Homo sapiens				5 % sheep blood-enriched Columbia agar (BioMerieux)	2 days	37

Taxonmaps

69479

Global distribution of 16S sequence LN846908 (>99% sequence identity) for Fournierella massiliensis subclade from Microbeatlas

Aquatic Samples	964
Soil Samples	282
Animal Samples	54181
Plant Samples	109
Total Samples	55536

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
42874	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM434526v1 assembly for Allofournierella massiliensis DSM 100451	contig	GCA_004345265	1650663.6	2788499850	1650663	62.39

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
42874	Ruminococcaceae bacterium AT2 partial 16S rRNA gene, strain AT2	LN846908	1491		1650663

GC content

@ref	GC-content (mol%)	Method
43184	56.8	genome sequence analysis
42874	56.8	sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM434526v1 assembly for Allofournierella massiliensis DSM 100451
NCBI: GCA_004345265

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate anaerobe	98.93	no
125439	motility	BacteriaNetⓘ	no	60.86	no
125439	gram_stain	BacteriaNetⓘ	negative	47.21	no
125439	spore_formation	BacteriaNetⓘ	no	77.05	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Fournierella massiliensis strain DSM 100451
PATRIC: 1650663.6

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	59.94	yes
125438	anaerobic	anaerobicⓘ	yes	88.81	yes
125438	spore-forming	spore-formingⓘ	no	65.56	yes
125438	aerobic	aerobicⓘ	no	96.51	no
125438	thermophilic	thermophileⓘ	no	93.89	yes
125438	flagellated	motile2+ⓘ	no	86.13	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Fournierella massiliensis gen. nov., sp. nov., a new human-associated member of the family Ruminococcaceae.	Togo AH, Durand G, Khelaifia S, Armstrong N, Robert C, Cadoret F, Di Pinto F, Delerce J, Levasseur A, Raoult D, Million M	Int J Syst Evol Microbiol	10.1099/ijsem.0.001826	2017

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#42874	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100451
#43184	Amadou Hamidou Togo, Guillaume Durand, Saber Khelaifia, Nicholas Armstrong, Catherine Robert, Frederic Cadoret, Fabrizio Di Pinto, Jeremy Delerce, Anthony Levasseur, Didier Raoult, Matthieu Million: Fournierella massiliensis gen. nov., sp. nov., a new human-associated member of the family Ruminococcaceae. IJSEM 67: 1393 - 1399 2017 ( DOI 10.1099/ijsem.0.001826 , PubMed 28126042 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68380	Automatically annotated from API rID32A .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Allofournierella massiliensis (DSM 100451, CSUR P2014, AT2)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API rID32A

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_004345265

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media