Name: Fournierella massiliensis AT2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140267

Type strain:

Species: Fournierella massiliensis

Strain Designation: AT2

Culture col. no.: DSM 100451, CSUR P2014

Strain history: <- S. Khelaifia, URMITE, Univ. Méditerranée, Marseille, France; AT2

NCBI tax ID(s): 1650663 (species)

SI-ID 403978

DSM 100451

Section

Fournierella massiliensis AT2 is an anaerobe, thermophilic, Gram-negative bacterium that was isolated from human stool specimen, healthy 28-year-old French man.

Gram-negative
rod-shaped
anaerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Oscillospiraceae*
	Genus	*Fournierella*
	Species	**Fournierella massiliensis**
	Full Scientific Name (LPSN)	Fournierella massiliensis Togo et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43184]	Gram stain	negative

[Ref.: #43184]	Cell length	2 µm

[Ref.: #43184]	Cell width	0.5 µm

[Ref.: #43184]	Cell shape	rod-shaped

[Ref.: #43184]	Motility	no

[Ref.: #43184]	Colony size	0.3-1 mm
[Ref.: #43184]	Colony color	white
[Ref.: #43184]	Cultivation medium used	5 % sheep blood-enriched Columbia agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43184]

Culture medium

5 % sheep blood-enriched Columbia agar

[Ref.: #43184]

Culture medium growth

[Ref.: #42874]

Culture medium

WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339)

[Ref.: #42874]

Culture medium growth

[Ref.: #42874]

Culture medium link

Medium recipe at MediaDive

[Ref.: #42874]

Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #42874]		growth	37 °C
[Ref.: #43184]	no	growth	55 °C
[Ref.: #43184]		growth	45 °C
[Ref.: #43184]		optimum	37 °C
[Ref.: #43184]		growth	28 °C

[Ref.: #42874]	Temperature range	mesophilic
[Ref.: #43184]	Temperature range	thermophilic
[Ref.: #43184]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43184]			optimum	7.0-7.3
[Ref.: #43184]			growth	6-8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43184]

Oxygen tolerance

anaerobe

[Ref.: #43184]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43184]		NaCl	no	growth	10	g/L

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43184]	amygdalin ChEBI	-	builds acid from
[Ref.: #43184]	arbutin ChEBI	-	builds acid from
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #43184]	cellobiose ChEBI	-	builds acid from
[Ref.: #43184]	cellulose ChEBI	-	assimilation
[Ref.: #43184]	D-arabinose ChEBI	-	builds acid from
[Ref.: #43184]	D-arabitol ChEBI	-	builds acid from
[Ref.: #43184]	D-fructose ChEBI	+	builds acid from
[Ref.: #43184]	D-fucose ChEBI	-	builds acid from
[Ref.: #43184]	D-galactose ChEBI	+	builds acid from
[Ref.: #43184]	D-glucose ChEBI	+	builds acid from
[Ref.: #43184]	D-glucose ChEBI	+	assimilation
[Ref.: #43184]	D-mannitol ChEBI	-	builds acid from
[Ref.: #43184]	D-mannitol ChEBI	-	assimilation
[Ref.: #43184]	D-mannose ChEBI	+	builds acid from
[Ref.: #43184]	D-mannose ChEBI	+	assimilation
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #43184]	D-ribose ChEBI	-	builds acid from
[Ref.: #43184]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #43184]	D-sorbitol ChEBI	-	assimilation
[Ref.: #43184]	D-tagatose ChEBI	-	builds acid from
[Ref.: #43184]	D-xylose ChEBI	-	builds acid from
[Ref.: #43184]	D-xylose ChEBI	-	assimilation
[Ref.: #43184]	erythritol ChEBI	-	builds acid from
[Ref.: #43184]	esculin ferric citrate	+	builds acid from
[Ref.: #43184]	esculin ferric citrate	+	assimilation
[Ref.: #43184]	galactitol ChEBI	-	builds acid from
[Ref.: #43184]	gelatin ChEBI	-	hydrolysis
[Ref.: #43184]	gentiobiose ChEBI	-	builds acid from
[Ref.: #43184]	glycerol ChEBI	+	builds acid from
[Ref.: #43184]	glycerol ChEBI	+	assimilation
[Ref.: #43184]	glycogen ChEBI	-	builds acid from
[Ref.: #43184]	inulin ChEBI	-	builds acid from
[Ref.: #43184]	L-arabinose ChEBI	-	builds acid from
[Ref.: #43184]	L-arabinose ChEBI	-	assimilation
[Ref.: #43184]	L-arabitol ChEBI	-	builds acid from
[Ref.: #43184]	L-fucose ChEBI	-	builds acid from
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #43184]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #43184]	L-rhamnose ChEBI	-	assimilation
[Ref.: #43184]	L-sorbose ChEBI	-	builds acid from
[Ref.: #43184]	L-xylose ChEBI	-	builds acid from
[Ref.: #43184]	lactose ChEBI	+	builds acid from
[Ref.: #43184]	lactose ChEBI	+	assimilation
[Ref.: #43184]	maltose ChEBI	+	builds acid from
[Ref.: #43184]	maltose ChEBI	+	assimilation
[Ref.: #43184]	melezitose ChEBI	+	builds acid from
[Ref.: #43184]	melezitose ChEBI	+	assimilation
[Ref.: #43184]	melibiose ChEBI	+	builds acid from
[Ref.: #43184]	methyl alpha-D-glucopyranoside	+	builds acid from
[Ref.: #43184]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #43184]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #43184]	myo-inositol ChEBI	-	builds acid from
[Ref.: #43184]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #43184]	potassium 2-dehydro-D-gluconate	-	builds acid from
[Ref.: #43184]	potassium 5-dehydro-D-gluconate	+	builds acid from
[Ref.: #43184]	potassium gluconate ChEBI	-	builds acid from
[Ref.: #43184]	raffinose ChEBI	+	builds acid from
[Ref.: #43184]	raffinose ChEBI	+	assimilation
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #43184]	ribitol ChEBI	-	builds acid from
[Ref.: #43184]	salicin ChEBI	+	builds acid from
[Ref.: #43184]	salicin ChEBI	+	assimilation
[Ref.: #43184]	starch ChEBI	-	builds acid from
[Ref.: #43184]	sucrose ChEBI	+	builds acid from
[Ref.: #43184]	sucrose ChEBI	+	assimilation
[Ref.: #43184]	trehalose ChEBI	-	builds acid from
[Ref.: #43184]	trehalose ChEBI	-	assimilation
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #43184]	turanose ChEBI	+	builds acid from
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #43184]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43184]		indole ChEBI	no
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #43184]	acid phosphatase	+	3.1.3.2
[Ref.: #68380]	alanine arylamidase	-	3.4.11.2	* from API rID32A
[Ref.: #43184]	alkaline phosphatase	-	3.1.3.1
[Ref.: #68380]	alkaline phosphatase	-	3.1.3.1	* from API rID32A
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #43184]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #43184]	alpha-fucosidase	-	3.2.1.51
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #43184]	alpha-galactosidase	+	3.2.1.22
[Ref.: #68380]	alpha-galactosidase	+	3.2.1.22	* from API rID32A
[Ref.: #43184]	alpha-glucosidase	+	3.2.1.20
[Ref.: #68380]	alpha-glucosidase	+	3.2.1.20	* from API rID32A
[Ref.: #43184]	alpha-mannosidase	-	3.2.1.24
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #43184]	beta-galactosidase	-	3.2.1.23
[Ref.: #68380]	beta-galactosidase	+	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #43184]	beta-glucosidase	+	3.2.1.21
[Ref.: #68380]	beta-glucosidase	+	3.2.1.21	* from API rID32A
[Ref.: #43184]	beta-glucuronidase	-	3.2.1.31
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #43184]	cystine arylamidase	-	3.4.11.3
[Ref.: #43184]	esterase (C 4)	+
[Ref.: #43184]	esterase Lipase (C 8)	+
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	-		* from API rID32A
[Ref.: #43184]	leucine arylamidase	-	3.4.11.1
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #43184]	lipase	-
[Ref.: #43184]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32A
[Ref.: #43184]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #43184]	trypsin	-	3.4.21.4
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #43184]	urease	-	3.5.1.5
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
[Ref.: #43184]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #42874]	12887	-	-	+	+	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #42874]	Sample type/isolated from	human stool specimen, healthy 28-year-old French man
[Ref.: #42874]	Country	France
[Ref.: #42874]	Country ISO 3 Code	FRA
[Ref.: #42874]	Continent	Europe

[Ref.: #43184]	Sample type/isolated from	fresh stool sample from a healthy 28-year-old French man
[Ref.: #43184]	Enrichment culture	5 % sheep blood-enriched Columbia agar (BioMerieux)
[Ref.: #43184]	Enrichment culture duration	2 days
[Ref.: #43184]	Enrichment culture temperature	37 ̊C
* marker position based on {}

Isolation sources categories

#Infection	#Patient	#Specimen
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)
#Host	#Human	#Male

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LN846908 (>99% sequence identity) for Fournierella massiliensis subclade from Microbeatlas

Aquatic Samples	964
Soil Samples	282
Animal Samples	54181
Plant Samples	109
Total Samples	55536

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #42874]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #42874]	Ruminococcaceae bacterium AT2 partial 16S rRNA gene, strain AT2	LN846908	1491	ENA	1650663 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Fournierella massiliensis DSM 100451	GCA_004345265	contig	GenBank	1650663 tax ID	*
[Ref.: #66792]	Fournierella massiliensis strain DSM 100451	1650663.6	wgs	PATRIC	1650663 tax ID	*
[Ref.: #66792]	Fournierella massiliensis DSM 100451	2788499850	draft	JGI IMG/M	1650663 tax ID	*

[Ref.: #43184]	GC-content	56.8 mol%	genome sequence analysis
[Ref.: #42874]	GC-content	56.8 mol%	sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Fournierella massiliensis strain DSM 100451 PATRIC: 1650663.6
[Ref.: #69480]	motile	no	91.42	yes
[Ref.: #69480]	gram-positive	yes	86.198	no
[Ref.: #69480]	anaerobic	yes	99.583	no
[Ref.: #69480]	aerobic	no	98.695	yes
[Ref.: #69480]	halophile	no	85.57	no
[Ref.: #69480]	spore-forming	no	91.002	yes
[Ref.: #69480]	thermophile	no	95.126	no
[Ref.: #69480]	glucose-util	yes	87.918	yes
[Ref.: #69480]	flagellated	no	96.319	no
[Ref.: #69480]	glucose-ferment	yes	76.668	no

Availability in culture collections External links

[Ref.: #42874]

Culture collection no.

DSM 100451, CSUR P2014

[Ref.: #96573]

SI-ID 403978

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Fournierella massiliensis gen. nov., sp. nov., a new human-associated member of the family Ruminococcaceae.	Togo AH, Durand G, Khelaifia S, Armstrong N, Robert C, Cadoret F, Di Pinto F, Delerce J, Levasseur A, Raoult D, Million M	Int J Syst Evol Microbiol	10.1099/ijsem.0.001826	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#42874

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100451

#43184

Amadou Hamidou Togo, Guillaume Durand, Saber Khelaifia, Nicholas Armstrong, Catherine Robert, Frederic Cadoret, Fabrizio Di Pinto, Jeremy Delerce, Anthony Levasseur, Didier Raoult, Matthieu Million: Fournierella massiliensis gen. nov., sp. nov., a new human-associated member of the family Ruminococcaceae. IJSEM 67: 1393 - 1399 2017 ( DOI 10.1099/ijsem.0.001826 , PubMed 28126042 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#96573

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403978.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Fournierella massiliensis in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF

Fournierella massiliensis strain DSM 100451

Change proposal

Change proposal