Mesobacillus zeae | Type strain | DSM 103964, CCM 8726, JJ-247, LMG 29876

Strain identifier

BacDive ID: 140266

Type strain:

Species: Mesobacillus zeae

Culture col. no.: DSM 103964, CCM 8726, JJ-247 , LMG 29876

Strain history: <- P. Kämpfer, Justus-Liebig-Universität, Giessen, Germany <- J. A. McInroy; JJ-247

Section

Name and taxonomic classification
Morphology
Culture and growth conditions
Physiology and metabolism
Isolation, sampling and environmental information
Safety information
Sequence information

External links

Mesobacillus zeae DSM 103964 is an aerobe, thermophilic, gram-positive bacterium that forms circular colonies and was isolated from rhizosphere of field-grown Zea mays plants.

colony-forming
gram-positive
rod-shaped
aerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification.Name and taxonomic classification

	Last LPSN update	23-11-2020 (DD-MM-YYYY)
	Domain	Bacteria
	Phylum	Firmicutes
	Class	Bacilli
	Order	Caryophanales
	Family	Bacillaceae
	Genus	Mesobacillus
	Species	Mesobacillus zeae
	Full Scientific Name (PNU)	Mesobacillus zeae (Kämpfer et al. 2017) Patel and Gupta 2020

Synonym

Bacillus zeae

Information on morphological and physiological propertiesMorphology

[Ref.: #43173]	Gram stain	positive
[Ref.: #43173]	Cell length	3.5 µm
[Ref.: #43173]	Cell width	1 µm
[Ref.: #43173]	Cell shape	rod-shaped
[Ref.: #43173]	Motility	no

[Ref.: #43173]	Colony color	beige
[Ref.: #43173]	Colony shape	circular
[Ref.: #43173]	Cultivation medium used	nutritient agar (NA)

Information on culture and growth conditionsCulture and growth conditions

[Ref.: #43173]

Culture medium

nutritient agar (NA)

[Ref.: #43173]

Culture medium growth

[Ref.: #43173]

Culture medium

brain heart infusion agar

[Ref.: #43173]

Culture medium growth

[Ref.: #43173]

Culture medium

tryptic soy agar (TSA)

[Ref.: #43173]

Culture medium growth

[Ref.: #43173]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #43173]

Culture medium growth

[Ref.: #42873]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #42873]

Culture medium growth

[Ref.: #42873]

Culture medium link

medium recipe provided by DSMZ

[Ref.: #42873]

Culture medium

PLATE COUNT AGAR (DSMZ Medium 464)

[Ref.: #42873]

Culture medium growth

[Ref.: #42873]

Culture medium link

medium recipe provided by DSMZ

		Temperature
[Ref.: #42873]	growth	30 ̊C
[Ref.: #43173]	maximum	50 ̊C
[Ref.: #43173]	minimum	10 ̊C
[Ref.: #43173]	optimum	28-30 ̊C
[Ref.: #43173]	growth	20-30 ̊C

[Ref.: #42873]	Temperature range	mesophilic
[Ref.: #43173]	Temperature range	thermophilic
[Ref.: #43173]	Temperature range	psychrophilic
[Ref.: #43173]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43173]			optimum	7
[Ref.: #43173]			growth	6.0-10.0

Information on physiology and metabolismPhysiology and metabolism

[Ref.: #43173]

Oxygen tolerance

aerobe

[Ref.: #43173]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43173]		NaCl		growth	0-8.0	%(w/v)
[Ref.: #43173]		NaCl		optimum	3-5	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43173]	2-deoxythymidine-5'-4-nitrophenyl phosphate	-	hydrolysis
[Ref.: #43173]	2-oxoglutarate ChEBI	-	carbon source
[Ref.: #43173]	3-hydroxybenzoate ChEBI	-	carbon source
[Ref.: #43173]	3-hydroxybutyrate ChEBI	-	carbon source
[Ref.: #43173]	3-O-methyl alpha-D-glucopyranoside	-	builds acid from
[Ref.: #43173]	4-hydroxybenzoate ChEBI	-	carbon source
[Ref.: #43173]	4-nitrophenyl alpha-D-glucopyranoside ChEBI	+	hydrolysis
[Ref.: #43173]	4-nitrophenyl beta-D-galactopyranoside ChEBI	-	hydrolysis
[Ref.: #43173]	4-nitrophenyl beta-D-glucopyranoside ChEBI	-	hydrolysis
[Ref.: #43173]	4-nitrophenyl beta-D-glucuronide ChEBI	-	hydrolysis
[Ref.: #43173]	4-nitrophenyl beta-D-xylopyranoside ChEBI	-	hydrolysis
[Ref.: #43173]	acetate ChEBI	+	carbon source
[Ref.: #43173]	adipate ChEBI	-	carbon source
[Ref.: #43173]	azelaate ChEBI	-	carbon source
[Ref.: #43173]	beta-alanine ChEBI	-	carbon source
[Ref.: #43173]	bis-4-nitrophenyl phosphate ChEBI	+	hydrolysis
[Ref.: #43173]	bis-4-nitrophenyl-phosphorylcholine ChEBI	-	hydrolysis
[Ref.: #43173]	casein	-	hydrolysis
[Ref.: #43173]	cellobiose ChEBI	-	carbon source
[Ref.: #43173]	cis-aconitate ChEBI	-	carbon source
[Ref.: #43173]	citrate ChEBI	-	carbon source
[Ref.: #43173]	D-arabitol ChEBI	-	builds acid from
[Ref.: #43173]	D-fructose ChEBI	-	carbon source
[Ref.: #43173]	D-galactose ChEBI	-	carbon source
[Ref.: #43173]	D-glucose ChEBI	+	builds acid from
[Ref.: #43173]	D-glucose ChEBI	+	carbon source
[Ref.: #43173]	D-mannitol ChEBI	-	builds acid from
[Ref.: #43173]	D-mannitol ChEBI	-	carbon source
[Ref.: #43173]	D-mannose ChEBI	-	carbon source
[Ref.: #43173]	D-ribose ChEBI	-	carbon source
[Ref.: #43173]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #43173]	D-sorbitol ChEBI	-	carbon source
[Ref.: #43173]	D-xylose ChEBI	-	builds acid from
[Ref.: #43173]	D-xylose ChEBI	-	carbon source
[Ref.: #43173]	dna ChEBI	-	hydrolysis
[Ref.: #43173]	erythritol ChEBI	-	builds acid from
[Ref.: #43173]	fumarate ChEBI	-	carbon source
[Ref.: #43173]	galactitol ChEBI	-	builds acid from
[Ref.: #43173]	gamma-aminobutyric acid ChEBI	-	carbon source
[Ref.: #43173]	gamma-L-glutamate-4-nitroanilide	-	hydrolysis
[Ref.: #43173]	gelatin ChEBI	-	hydrolysis
[Ref.: #43173]	gluconate ChEBI	-	carbon source
[Ref.: #43173]	glutarate ChEBI	-	carbon source
[Ref.: #43173]	glycerol ChEBI	-	carbon source
[Ref.: #43173]	itaconate ChEBI	-	carbon source
[Ref.: #43173]	L-alanine ChEBI	-	carbon source
[Ref.: #43173]	L-alanine 4-nitroanilide	-	hydrolysis
[Ref.: #43173]	L-arabinose ChEBI	-	builds acid from
[Ref.: #43173]	L-arabinose ChEBI	-	carbon source
[Ref.: #43173]	L-aspartate ChEBI	-	carbon source
[Ref.: #43173]	L-histidine ChEBI	-	carbon source
[Ref.: #43173]	L-leucine ChEBI	-	carbon source
[Ref.: #43173]	L-ornithine ChEBI	-	carbon source
[Ref.: #43173]	L-phenylalanine ChEBI	-	carbon source
[Ref.: #43173]	L-proline ChEBI	-	carbon source
[Ref.: #43173]	L-proline-4-nitroanilide	-	hydrolysis
[Ref.: #43173]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #43173]	L-rhamnose ChEBI	-	carbon source
[Ref.: #43173]	L-serine ChEBI	-	carbon source
[Ref.: #43173]	L-tryptophan ChEBI	-	carbon source
[Ref.: #43173]	lactate ChEBI	-	carbon source
[Ref.: #43173]	lactose ChEBI	-	builds acid from
[Ref.: #43173]	maltitol ChEBI	-	carbon source
[Ref.: #43173]	maltose ChEBI	-	builds acid from
[Ref.: #43173]	maltose ChEBI	-	carbon source
[Ref.: #43173]	melibiose ChEBI	-	builds acid from
[Ref.: #43173]	melibiose ChEBI	-	carbon source
[Ref.: #43173]	mesaconate ChEBI	-	carbon source
[Ref.: #43173]	myo-inositol ChEBI	-	builds acid from
[Ref.: #43173]	myo-inositol ChEBI	-	carbon source
[Ref.: #43173]	N-acetylgalactosamine ChEBI	-	carbon source
[Ref.: #43173]	N-acetylglucosamine ChEBI	-	carbon source
[Ref.: #43173]	phenylacetate ChEBI	-	carbon source
[Ref.: #43173]	propionate ChEBI	+	carbon source
[Ref.: #43173]	putrescine ChEBI	-	carbon source
[Ref.: #43173]	pyruvate ChEBI	-	carbon source
[Ref.: #43173]	raffinose ChEBI	-	builds acid from
[Ref.: #43173]	ribitol ChEBI	-	builds acid from
[Ref.: #43173]	ribitol ChEBI	-	carbon source
[Ref.: #43173]	salicin ChEBI	-	builds acid from
[Ref.: #43173]	salicin ChEBI	-	carbon source
[Ref.: #43173]	starch ChEBI	-	hydrolysis
[Ref.: #43173]	suberic acid ChEBI	-	carbon source
[Ref.: #43173]	sucrose ChEBI	+	builds acid from
[Ref.: #43173]	sucrose ChEBI	-	carbon source
[Ref.: #43173]	trans-aconitate ChEBI	-	carbon source
[Ref.: #43173]	trehalose ChEBI	-	builds acid from
[Ref.: #43173]	tyrosine ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43173]		hydrogen sulfide ChEBI	no
[Ref.: #43173]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43173]	arginine dihydrolase	-	3.5.3.6
[Ref.: #43173]	beta-galactosidase	-	3.2.1.23
[Ref.: #43173]	catalase	+	1.11.1.6
[Ref.: #43173]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43173]	lysine decarboxylase	-	4.1.1.18
[Ref.: #43173]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #43173]	urease	-	3.5.1.5

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43173]

Fatty acid	Percentage
C15:0 iso	41.9
C17:1 iso ω10c	9.3
C17:0 iso	9.1
C16:0 iso	7.7
C16:1ω7c OH	4.9
C15:0 anteiso	4.8
C17:0 anteiso	4.5
iso-C17:1 l / anteiso-C17:1 B	4.3
C16:1ω11c	3.6
C16:0	3.1
C15:0	2.7
C14:0 iso	2.5
C15:1 iso F	1.5

Information on isolation source, the sampling and environmental conditionsIsolation, sampling and environmental information

[Ref.: #42873]	Sample type/isolated from	rhizosphere of field-grown Zea mays plants
[Ref.: #42873]	Host species	Zea mays
[Ref.: #42873]	Geographic location (country and/or sea, region)	Nebraska, Dunbar
[Ref.: #42873]	Country	USA
[Ref.: #42873]	Country ISO 3 Code	USA
[Ref.: #42873]	Continent	North America

[Ref.: #43173]	Sample type/isolated from	rhizosphere of maize plants
[Ref.: #43173]	Geographic location (country and/or sea, region)	field in Dunbar, Nebraska
[Ref.: #43173]	Country	USA
[Ref.: #43173]	Country ISO 3 Code	USA
[Ref.: #43173]	Continent	North America
[Ref.: #43173]	Enrichment culture	nutrient agar (NA; Sigma-Aldrich)
[Ref.: #43173]	Enrichment culture duration	2 days
[Ref.: #43173]	Enrichment culture temperature	30 ̊C
[Ref.: #43173]	Isolation/enrichment procedure	serial dilution plating technique

Isolation sources categories

#Engineered	#Agriculture	#Field
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Rhizosphere

What are isolation sources categories?

* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hostsSafety information

[Ref.: #42873]

Biosafety level

Risk group (German classification)

Information on genomic background e.g. entries in nucleic sequence databassSequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #42873]	Bacillus zeae strain JJ-247 16S ribosomal RNA gene, partial sequence	KY114944	1473	ENA	1917180 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Bacillus zeae JJ-247	GCA_003570705	contig	GenBank	1917180 tax ID
[Ref.: #66792]	Bacillus zeae strain JJ-247	1917180.3	wgs	PATRIC	1917180 tax ID

[Ref.: #43173]	GC-content	44.5 mol%	Thermal denaturation, fluorometry
[Ref.: #42873]	GC-content	44.5 mol%	fluorimetric

Availability in culture collectionsExternal links

[Ref.: #42873]

Culture collection no.

DSM 103964, CCM 8726, JJ-247 , LMG 29876

References

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#42873

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 103964

#43173

Peter Kämpfer, Hans-Jürgen Busse, John A. McInroy, Chia-Hui Hu, Joseph W. Kloepper, Stefanie P. Glaeser: Bacillus zeae sp. nov., isolated from the rhizosphere of Zea mays. IJSEM 67: 1241 - 1246 2017 ( DOI 10.1099/ijsem.0.001791 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These References are textmined

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Mesobacillus zeae in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF