Azospirillum thiophilum (DSM 21654, VKM B-2513, Bv-S)

Name: Azospirillum thiophilum (DSM 21654, VKM B-2513, Bv-S)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 14000

Type strain

Strain Designation Bv-S

Culture col. no. DSM 21654 VKM B-2513 Bv-S

NCBI tax ID(s) 528244

Special Collections DSMZ Literature strains

Links

Azospirillum thiophilum Bv-S is an aerobe, mesophilic, Gram-negative prokaryote that was isolated from bacterial mat of a sulfide mineral spring.

Gram-negative motile rod-shaped aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 405148

VKM B-2513,DSM 21654

@ref 20215

Last LPSN update 2025-12-16 09:50:14

Domain Pseudomonadati

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Rhodospirillales

Family Azospirillaceae

Genus Azospirillum

Species Azospirillum thiophilum

Full scientific name Azospirillum thiophilum Lavrinenko et al. 2010

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
29693	negative	3.6-7 µm	1.1-2 µm	rod-shaped
125438	negative				98.5
125439	negative				98

Pigmentation

29693

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
15869	HETEROTROPHIC MEDIUM H3P (DSMZ Medium 428)		Medium recipe at MediaDive	Name: HETEROTROPHIC MEDIUM H3P (DSMZ Medium 428) Composition: Agar 19.802 g/l Na2HPO4 x 2 H2O 2.87129 g/l KH2PO4 2.27723 g/l D-Glucose 1.9802 g/l Na-lactate 0.990099 g/l Na-pyruvate 0.990099 g/l D-Mannitol 0.990099 g/l DL-Malate 0.990098 g/l Na-acetate 0.990098 g/l Yeast extract 0.990098 g/l NH4Cl 0.990098 g/l Disodium succinate 0.990098 g/l MgSO4 x 7 H2O 0.495049 g/l Ferric ammonium citrate 0.049505 g/l CaCl2 x 2 H2O 0.00990098 g/l NaVO3 x H2O 0.00495049 g/l Thiamine-HCl x 2 H2O 0.00247525 g/l Nicotinic acid 0.00247525 g/l Pyridoxine hydrochloride 0.00247525 g/l Calcium pantothenate 0.00247525 g/l H3BO3 0.00148515 g/l CoCl2 x 6 H2O 0.000990099 g/l Riboflavin 0.00049505 g/l ZnSO4 x 7 H2O 0.00049505 g/l MnCl2 x 4 H2O 0.000148515 g/l Na2MoO4 x 2 H2O 0.000148515 g/l NiCl2 x 6 H2O 9.90099e-05 g/l CuCl2 x 2 H2O 4.95049e-05 g/l Vitamin B12 4.95049e-05 g/l Folic acid 9.90099e-06 g/l Biotin 4.9505e-06 g/l Distilled water
15869	R2A MEDIUM (DSMZ Medium 830)		Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
15869	positive	growth	28	mesophilic
29693	positive	growth	15-40
29693	positive	optimum	37	mesophilic

Culture pH

@ref	Ability	Type	PH	PH range
29693	positive	optimum	7.5
29693	positive	growth	6.5-8.5	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
29693	aerobe
125439	obligate aerobe	99.1

Spore formation

@ref	Spore formation	Confidence
125439		97.3

Halophily

@ref	Salt	Growth	Tested relation	Concentration
29693	NaCl	positive	growth	0-3 %
29693	NaCl	positive	optimum	0-3 %

Observation

29693

Observationaggregates in chains

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
29693	30089 ChEBI	acetate	+	carbon source
29693	16449 ChEBI	alanine	+	carbon source
29693	22599 ChEBI	arabinose	+	carbon source
29693	22653 ChEBI	asparagine	+	carbon source
29693	35391 ChEBI	aspartate	+	carbon source
29693	28260 ChEBI	galactose	+	carbon source
29693	17234 ChEBI	glucose	+	carbon source
29693	17306 ChEBI	maltose	+	carbon source
29693	17632 ChEBI	nitrate	+	reduction
29693	28044 ChEBI	phenylalanine	+	carbon source
29693	15361 ChEBI	pyruvate	+	carbon source

Enzymes

@ref	Value	Activity	Ec
29693	catalase	+	1.11.1.6
29693	cytochrome oxidase	+	1.9.3.1

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Spring
#Host	#Microbial	#Bacteria
#Condition	#Sulfuric	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
15869	bacterial mat of a sulfide mineral spring	North Caucasus region, Stavropol Krai, 'Shameless Baths'	Russia	RUS	Europe

Taxonmaps

69479

Global distribution of 16S sequence EU678791 (>99% sequence identity) for Azospirillum thiophilum subclade from Microbeatlas

Aquatic Samples	3745
Soil Samples	1756
Animal Samples	868
Plant Samples	721
Total Samples	7090

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
15869	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM130559v1 assembly for Azospirillum thiophilum BV-S	complete	GCA_001305595	528244.4	2645728001	528244	94.24
66792	ASM96082v1 assembly for Azospirillum thiophilum DSM 21654	contig	GCA_000960825	528244.3	2630968760	528244	73.51

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
15869	Azospirillum thiophilum strain BV-S 16S ribosomal RNA gene, partial sequence	EU678791	1316		528244

GC content

@ref	GC-content (mol%)	Method
15869	67	thermal denaturation, midpoint method (Tm)
29693	67

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Azospirillum thiophilum strain BV-S
PATRIC: 528244.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	97.30	no
125439	motility	BacteriaNetⓘ	yes	74.50	no
125439	gram_stain	BacteriaNetⓘ	negative	98.00	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.10	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Azospirillum thiophilum BV-S
NCBI: GCA_001305595.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	98.50	yes
125438	anaerobic	anaerobicⓘ	no	89.29	yes
125438	aerobic	aerobicⓘ	yes	70.27	yes
125438	spore-forming	spore-formingⓘ	no	82.66	no
125438	thermophilic	thermophileⓘ	no	98.43	yes
125438	flagellated	motile2+ⓘ	yes	82.10	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete Genome Sequence of a Novel Azospirillum Strain TA Isolated from Western Siberia Chernevaya Taiga Soil.	Rayko M, Kravchenko I, Lapidus A.	Microorganisms	10.3390/microorganisms12122599	2024
	Raman spectroscopic and TEM monitoring of selenite and selenate reduction by the bacterium Azospirillum thiophilum with the formation of selenium(0) nanoparticles: Effects of sulfate.	Tugarova AV, Vladimirova AA, Dyatlova YA, Kamnev AA.	Spectrochim Acta A Mol Biomol Spectrosc	10.1016/j.saa.2024.125463	2025
	Effect of the oil from the fatty tissues of Crocodylus siamensis on gut microbiome diversity and metabolism in mice.	Parunyakul K, Chuchoiy A, Kooltueon S, Puttagamnerd P, Srisuksai K, Santativongchai P, Pongchairerk U, Tulayakul P, E-Kobon T, Fungfuang W.	PLoS One	10.1371/journal.pone.0289073	2023
	Screening of rhizobacteria from monkey pod trees for plant growth promoters and evaluating the antifungal potential of the biosynthesized selenium nanoparticles.	Babajide AM, Adebami GE, Adebayo-Tayo BC.	Sci Rep	10.1038/s41598-025-96330-x	2025
Metabolism	Genomic insights into metabolic versatility of a lithotrophic sulfur-oxidizing diazotrophic Alphaproteobacterium Azospirillum thiophilum.	Orlova MV, Tarlachkov SV, Dubinina GA, Belousova EV, Tutukina MN, Grabovich MY.	FEMS Microbiol Ecol	10.1093/femsec/fiw199	2016
Genetics	First Azospirillum genome from aquatic environments: Whole-genome sequence of Azospirillum thiophilum BV-S(T), a novel diazotroph harboring a capacity of sulfur-chemolithotrophy from a sulfide spring.	Kwak Y, Shin JH.	Mar Genomics	10.1016/j.margen.2015.11.001	2016
Genetics	Biodegradation of sodium selenite by a highly tolerant strain Rhodococcus qingshengii PM1: Biochemical characterization and comparative genome analysis.	Wang Z, Hou X, Guo Z, Lei X, Peng M.	Curr Res Microb Sci	10.1016/j.crmicr.2025.100426	2025
	Characterization and biological activity of selenium nanoparticles biosynthesized by Yarrowia lipolytica.	Lashani E, Moghimi H, Turner RJ, Amoozegar MA.	Microb Biotechnol	10.1111/1751-7915.70013	2024
	Effect of Bacteria from the Genus Azospirillum on Oxidative Stress Levels in Wheat Triticum aestivum L. in the Presence of Copper, Nickel, and Lead.	Gureeva MV, Kirillova MS, Trandina VA, Kryukova VA, Eremina AA, Alimova AA, Grabovich MY, Gureev AP.	Microorganisms	10.3390/microorganisms13020334	2025
	Complete Genome Sequence of a Strain of Azospirillum thiophilum Isolated from a Sulfide Spring.	Fomenkov A, Vincze T, Grabovich M, Anton BP, Dubinina G, Orlova M, Belousova E, Roberts RJ.	Genome Announc	10.1128/genomea.01521-15	2016
	Simultaneous Raman and infrared spectroscopy: a novel combination for studying bacterial infections at the single cell level.	Lima C, Ahmed S, Xu Y, Muhamadali H, Parry C, McGalliard RJ, Carrol ED, Goodacre R.	Chem Sci	10.1039/d2sc02493d	2022
	Composition and biodiversity of soil and root-associated microbiome in Vitis vinifera cultivar Lambrusco distinguish the microbial terroir of the Lambrusco DOC protected designation of origin area on a local scale.	Nanetti E, Palladino G, Scicchitano D, Trapella G, Cinti N, Fabbrini M, Cozzi A, Accetta G, Tassini C, Iannaccone L, Candela M, Rampelli S.	Front Microbiol	10.3389/fmicb.2023.1108036	2023
	Biogenic Selenium Nanoparticles: A Fine Characterization to Unveil Their Thermodynamic Stability.	Piacenza E, Presentato A, Ferrante F, Cavallaro G, Alduina R, Chillura Martino DF.	Nanomaterials (Basel)	10.3390/nano11051195	2021
Transcriptome	Selenite Reduction by Proteus sp. YS02: New Insights Revealed by Comparative Transcriptomics and Antibacterial Effectiveness of the Biogenic Se⁰ Nanoparticles.	Wang Y, Ye Q, Sun Y, Jiang Y, Meng B, Du J, Chen J, Tugarova AV, Kamnev AA, Huang S.	Front Microbiol	10.3389/fmicb.2022.845321	2022
Pathogenicity	Determination of Plasmid pSN1216-29 Host Range and the Similarity in Oligonucleotide Composition Between Plasmid and Host Chromosomes.	Tokuda M, Suzuki H, Yanagiya K, Yuki M, Inoue K, Ohkuma M, Kimbara K, Shintani M.	Front Microbiol	10.3389/fmicb.2020.01187	2020
Metabolism	Methyl Selenol as a Precursor in Selenite Reduction to Se/S Species by Methane-Oxidizing Bacteria.	Eswayah AS, Hondow N, Scheinost AC, Merroun M, Romero-Gonzalez M, Smith TJ, Gardiner PHE.	Appl Environ Microbiol	10.1128/aem.01379-19	2019
Biotechnology	Nanoparticle classification, physicochemical properties, characterization, and applications: a comprehensive review for biologists.	Joudeh N, Linke D.	J Nanobiotechnology	10.1186/s12951-022-01477-8	2022
Genetics	Identification of Novel Genomic Islands in Liverpool Epidemic Strain of Pseudomonas aeruginosa Using Segmentation and Clustering.	Jani M, Mathee K, Azad RK.	Front Microbiol	10.3389/fmicb.2016.01210	2016
Genetics	Analysis of 1,000+ Type-Strain Genomes Substantially Improves Taxonomic Classification of Alphaproteobacteria.	Hordt A, Lopez MG, Meier-Kolthoff JP, Schleuning M, Weinhold LM, Tindall BJ, Gronow S, Kyrpides NC, Woyke T, Goker M.	Front Microbiol	10.3389/fmicb.2020.00468	2020
Metabolism	FTIR and Raman spectroscopic studies of selenium nanoparticles synthesised by the bacterium Azospirillum thiophilum.	Tugarova AV, Mamchenkova PV, Dyatlova YA, Kamnev AA	Spectrochim Acta A Mol Biomol Spectrosc	10.1016/j.saa.2017.11.050	2017
Phylogeny	Azospirillum agricola sp. nov., a nitrogen-fixing species isolated from cultivated soil.	Lin SY, Liu YC, Hameed A, Hsu YH, Huang HI, Lai WA, Young CC.	Int J Syst Evol Microbiol	10.1099/ijsem.0.000904	2016
Phylogeny	Azospirillum humicireducens sp. nov., a nitrogen-fixing bacterium isolated from a microbial fuel cell.	Zhou S, Han L, Wang Y, Yang G, Zhuang L, Hu P.	Int J Syst Evol Microbiol	10.1099/ijs.0.046813-0	2013
Phylogeny	Azospirillum thermophilum sp. nov., isolated from a hot spring.	Zhao ZL, Ming H, Ding CL, Ji WL, Cheng LJ, Niu MM, Zhang YM, Zhang LY, Meng XL, Nie GX	Int J Syst Evol Microbiol	10.1099/ijsem.0.003788	2020
Phylogeny	Azospirillum griseum sp. nov., isolated from lakewater.	Yang Y, Zhang R, Feng J, Wang C, Chen J	Int J Syst Evol Microbiol	10.1099/ijsem.0.003460	2019
Phylogeny	Azospirillum thiophilum sp. nov., a diazotrophic bacterium isolated from a sulfide spring.	Lavrinenko K, Chernousova E, Gridneva E, Dubinina G, Akimov V, Kuever J, Lysenko A, Grabovich M	Int J Syst Evol Microbiol	10.1099/ijs.0.018853-0	2010

References

#15869	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21654
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#26078	IJSEM 2832 2010 ( DOI 10.1099/ijs.0.018853-0 , PubMed 20081019 )
#29693	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26078
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Azospirillum thiophilum (DSM 21654, VKM B-2513, Bv-S)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001305595

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media