Azospirillum largimobile (DSM 1841, ATCC 29731, Sp RG6xx)

Name: Azospirillum largimobile (DSM 1841, ATCC 29731, Sp RG6xx)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13984

Type strain

Strain Designation Sp RG6xx

Culture col. no. DSM 1841 ATCC 29731 Sp RG6xx JCM 1228

NCBI tax ID(s) 52761

Special Collections DSMZ JCM

Links

Azospirillum largimobile Sp RG6xx is a mesophilic prokaryote that was isolated from wheat roots.

mesophilic 16S sequence

Name and taxonomic classification

SI-ID 39685

ATCC 29731,DSM 1841,JCM 1228,BCRC 15779,CCRC 15779

@ref 20215

Last LPSN update 2025-12-16 09:50:14

Domain Pseudomonadati

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Rhodospirillales

Family Azospirillaceae

Genus Azospirillum

Species Azospirillum largimobile

Full scientific name Azospirillum largimobile corrig. (Skerman et al. 1983) Ben Dekhil et al. 1997

Synonyms (2)

Conglomeromonas largomobilis
Azospirillum largomobile

BacDive ID	Other strains from **Azospirillum largimobile** (8)	Type strain
168718	A. largimobile UQM 2041, DSM 9441, ACM 2041 (type strain)
13982	A. largimobile Sp A3a, DSM 1838
13983	A. largimobile Sp RG2Oa, DSM 1840, ATCC 29708, JCM 1227
13986	A. largimobile Sp Col5, CCUG 12807, DSM 1860
13987	A. largimobile Sp Br10, DSM 2291, Br 10
13988	A. largimobile Sp USA 5b, DSM 2292
13989	A. largimobile Sp Col 2b, DSM 2293
13990	A. largimobile Sp Col 3, DSM 2294

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
900	MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3) (DSMZ Medium 81)	Medium recipe at MediaDive	Name: MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3) (DSMZ Medium 81) Composition: Agar 20.1005 g/l Na2HPO4 x 2 H2O 2.91457 g/l KH2PO4 2.31156 g/l NH4Cl 1.00503 g/l MgSO4 x 7 H2O 0.502512 g/l Ferric ammonium citrate 0.0502513 g/l CaCl2 x 2 H2O 0.0100503 g/l NaVO3 x H2O 0.00502512 g/l Calcium pantothenate 0.00251256 g/l Pyridoxine hydrochloride 0.00251256 g/l Nicotinic acid 0.00251256 g/l Thiamine-HCl x 2 H2O 0.00251256 g/l H3BO3 0.00150754 g/l CoCl2 x 6 H2O 0.00100503 g/l Riboflavin 0.000502513 g/l ZnSO4 x 7 H2O 0.000502513 g/l MnCl2 x 4 H2O 0.000150754 g/l Na2MoO4 x 2 H2O 0.000150754 g/l NiCl2 x 6 H2O 0.000100503 g/l CuCl2 x 2 H2O 5.02513e-05 g/l Vitamin B12 5.02513e-05 g/l Folic acid 1.00503e-05 g/l Biotin 5.02513e-06 g/l Distilled water
900	HETEROTROPHIC MEDIUM H3P (DSMZ Medium 428)	Medium recipe at MediaDive	Name: HETEROTROPHIC MEDIUM H3P (DSMZ Medium 428) Composition: Agar 19.802 g/l Na2HPO4 x 2 H2O 2.87129 g/l KH2PO4 2.27723 g/l D-Glucose 1.9802 g/l Na-lactate 0.990099 g/l Na-pyruvate 0.990099 g/l D-Mannitol 0.990099 g/l DL-Malate 0.990098 g/l Na-acetate 0.990098 g/l Yeast extract 0.990098 g/l NH4Cl 0.990098 g/l Disodium succinate 0.990098 g/l MgSO4 x 7 H2O 0.495049 g/l Ferric ammonium citrate 0.049505 g/l CaCl2 x 2 H2O 0.00990098 g/l NaVO3 x H2O 0.00495049 g/l Thiamine-HCl x 2 H2O 0.00247525 g/l Nicotinic acid 0.00247525 g/l Pyridoxine hydrochloride 0.00247525 g/l Calcium pantothenate 0.00247525 g/l H3BO3 0.00148515 g/l CoCl2 x 6 H2O 0.000990099 g/l Riboflavin 0.00049505 g/l ZnSO4 x 7 H2O 0.00049505 g/l MnCl2 x 4 H2O 0.000148515 g/l Na2MoO4 x 2 H2O 0.000148515 g/l NiCl2 x 6 H2O 9.90099e-05 g/l CuCl2 x 2 H2O 4.95049e-05 g/l Vitamin B12 4.95049e-05 g/l Folic acid 9.90099e-06 g/l Biotin 4.9505e-06 g/l Distilled water
900	AZOSPIRILLUM MEDIUM (DSMZ Medium 221)	Medium recipe at MediaDive	Name: AZOSPIRILLUM MEDIUM (DSMZ Medium 221) Composition: Agar 15.0 g/l Na malate 5.0 g/l Glucose 5.0 g/l (NH4)2SO4 1.0 g/l K2HPO4 0.25 g/l MgSO4 x 7 H2O 0.2 g/l NaCl 0.1 g/l Yeast extract 0.05 g/l CaCl2 x 2 H2O 0.02 g/l FeSO4 x 7 H2O 0.01 g/l MnSO4 x H2O 0.002 g/l Na2MoO4 x 2 H2O 0.001 g/l Biotin 0.0001 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
900	positive	growth	30	mesophilic
67770	positive	growth	30	mesophilic

Physiology and metabolism

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root (Rhizome)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
900	wheat roots		Brazil	BRA	Middle and South America
67770	Wheat	Passo Fundo	Brazil	BRA	Middle and South America

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
900	1	Risk group (German classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	A.lipoferum (ATCC29731) 16S rRNA gene	X79730	1333		193
67770	Azospirillum lipoferum gene for 16S rRNA, partial sequence, strain: JCM 1228	LC379117	1406		193

GC content

@ref	GC-content (mol%)	Method
67770	70	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Literature

References

#900	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 1841
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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