Name: Burkholderia puraquae HST 245
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 139753

Type strain:

Species: Burkholderia puraquae

Strain Designation: HST 245

Culture col. no.: DSM 103137, LMG 29660, Campa 1040

Strain history: <- A. Bosch, CINDEFI, CONICET-CCT, Universidad Nacional, La Plata Buenos Aires, Argentina; Campa 1040 <- P. Montanaro, Hospital de Ninos de la Santissima Trinidad de Cordoba, Argentina

NCBI tax ID(s): 1904757 (species)

SI-ID 404941

DSM 103137

Section

Burkholderia puraquae HST 245 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from haemodialysis water at Hospital de Niños de la Santísima Trinidad.

Gram-negative
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Burkholderiaceae*
	Genus	*Burkholderia*
	Species	**Burkholderia puraquae**
	Full Scientific Name (LPSN)	Burkholderia puraquae Martina et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65286]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.953

[Ref.: #65286]	Cell shape	rod-shaped

[Ref.: #69480]	Motility	yes Confidence in %93.054

[Ref.: #65286]	Type of hemolysis	gamma
[Ref.: #65286]	Colony color	yellow

Information on culture and growth conditions Culture and growth conditions

[Ref.: #42364]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #42364]

Culture medium growth

[Ref.: #42364]

Culture medium link

Medium recipe at MediaDive

[Ref.: #42364]

Culture medium composition

expand / minimize

[Ref.: #65286]

Culture medium

MacConkey agar

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Culture medium growth

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Culture medium

Burkholderia cepacia selective agar

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Culture medium growth

	Kind of temperature		Temperature
[Ref.: #42364]		growth	30 °C
[Ref.: #65286]		growth	30-37 °C
[Ref.: #65286]	no	growth	42 °C

[Ref.: #42364]	Temperature range	mesophilic
[Ref.: #65286]	Temperature range	mesophilic
[Ref.: #65286]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65286]

Oxygen tolerance

aerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.966

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65286]	adipate ChEBI	+	assimilation
[Ref.: #65286]	citrate ChEBI	+	assimilation
[Ref.: #65286]	D-gluconate ChEBI	+	assimilation
[Ref.: #65286]	D-mannitol ChEBI	+	assimilation
[Ref.: #65286]	D-mannose ChEBI	+	assimilation
[Ref.: #65286]	decanoate ChEBI	+	assimilation
[Ref.: #65286]	glucose ChEBI	+	assimilation
[Ref.: #65286]	glucose ChEBI	+	builds acid from
[Ref.: #65286]	L-arabinose ChEBI	+	assimilation
[Ref.: #65286]	L-malate ChEBI	+	assimilation
[Ref.: #65286]	lactose ChEBI	+	builds acid from
[Ref.: #65286]	maltose ChEBI	-	assimilation
[Ref.: #65286]	mannitol ChEBI	+	builds acid from
[Ref.: #65286]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #65286]	nitrate ChEBI	-	reduction
[Ref.: #65286]	phenylacetate ChEBI	+	assimilation
[Ref.: #65286]	xylose ChEBI	+	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #65286]	arginine dihydrolase	-	3.5.3.6
[Ref.: #65286]	beta-galactosidase	+	3.2.1.23
[Ref.: #65286]	cytochrome oxidase	+	1.9.3.1
[Ref.: #65286]	esterase	+
[Ref.: #65286]	gelatinase	+/-
[Ref.: #65286]	lysine decarboxylase	+	4.1.1.18
[Ref.: #65286]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #65286]	urease	-	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #42364]	Sample type/isolated from	haemodialysis water at Hospital de Niños de la Santísima Trinidad
[Ref.: #42364]	Geographic location (country and/or sea, region)	Córdoba
[Ref.: #42364]	Country	Argentina
[Ref.: #42364]	Country ISO 3 Code	ARG
[Ref.: #42364]	Continent	Middle and South America
[Ref.: #42364]	Geographic location	-31.4167°/64.1833°

[Ref.: #65286]	Sample type/isolated from	haemodialysis water reservoirs sample
[Ref.: #65286]	Geographic location (country and/or sea, region)	Hospital Santísima Trinidad in Córdoba city, Córdoba province
[Ref.: #65286]	Country	Argentina
[Ref.: #65286]	Country ISO 3 Code	ARG
[Ref.: #65286]	Continent	Middle and South America
[Ref.: #65286]	Enrichment culture	Burkholderia cepacia selective agar
[Ref.: #65286]	Enrichment culture duration	3 days
[Ref.: #65286]	Enrichment culture temperature	37 ̊C
[Ref.: #65286]	Isolation/enrichment procedure	Membrane filtration (pore size of membranes, 0.22 µm), was used as the concentrating technique. Following filtration, membranes were placed in enrichment medium and incubated at 37°C for 24 h. Positive cultures were inoculated on Burkholderia cepacia selective agar plates and incubated for 3 days at 37°C and for 2 additional days at room temperature
* marker position based on {}

Isolation sources categories

#Infection

#Medical environment

#Clinic

What are isolation sources categories?

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Global distribution of 16S sequence KX278717 (>99% sequence identity) for Burkholderia from Microbeatlas

Aquatic Samples	564
Soil Samples	1055
Animal Samples	1930
Plant Samples	1505
Total Samples	5054

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #42364]	Burkholderia puraquae strain CAMPA 1040 16S ribosomal RNA gene, partial sequence	KX278717	1452	ENA	1904757 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Burkholderia puraquae LMG 29660	GCA_902859845	contig	GenBank	1904757 tax ID	*
[Ref.: #66792]	Burkholderia puraquae strain LMG 29660	1904757.4	wgs	PATRIC	1904757 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Burkholderia puraquae strain LMG 29660 PATRIC: 1904757.3
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Burkholderia puraquae strain LMG 29660 PATRIC: 1904757.4
[Ref.: #69480]	motile	yes	87.475	no
[Ref.: #69480]	gram-positive	no	98.437	no
[Ref.: #69480]	anaerobic	no	98.738	yes
[Ref.: #69480]	aerobic	yes	91.693	yes
[Ref.: #69480]	halophile	no	92.14	no
[Ref.: #69480]	spore-forming	no	93.552	no
[Ref.: #69480]	glucose-util	yes	92.291	no
[Ref.: #69480]	flagellated	no	79.184	no
[Ref.: #69480]	thermophile	no	99.571	no
[Ref.: #69480]	glucose-ferment	no	86.594	no

Availability in culture collections External links

[Ref.: #42364]

Culture collection no.

DSM 103137, LMG 29660, Campa 1040

[Ref.: #96200]

SI-ID 404941

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Burkholderia puraquae sp. nov., a novel species of the Burkholderia cepacia complex isolated from hospital settings and agricultural soils.	Martina P, Leguizamon M, Prieto CI, Sousa SA, Montanaro P, Draghi WO, Stammler M, Bettiol M, de Carvalho CCCR, Palau J, Figoli C, Alvarez F, Benetti S, Lejona S, Vescina C, Ferreras J, Lasch P, Lagares A, Zorreguieta A, Leitao JH, Yantorno OM, Bosch A	Int J Syst Evol Microbiol	10.1099/ijsem.0.002293	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#42364

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 103137

#65286

Pablo Martina, Mariana Leguizamon, Claudia I. Prieto, Silvia A. Sousa, Patricia Montanaro, Walter O. Draghi, Maren Stämmler, Marisa Bettiol, Carla C. C. R. de Carvalho, Juliana Palau, Cecilia Figoli, Florencia Alvarez, Silvina Benetti, Sergio Lejona, Cecilia Vescina, Julián Ferreras, Peter Lasch, Antonio Lagares, Angeles Zorreguieta, Jorge H. Leitão, Osvaldo M. Yantorno, Alejandra Bosch: Burkholderia puraquae sp. nov., a novel species of the Burkholderia cepacia complex isolated from hospital settings and agricultural soils. IJSEM 68: 14 - 20 2018 ( DOI 10.1099/ijsem.0.002293 , PubMed 29095137 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#96200

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID404941.1 )

* These data were automatically processed and therefore are not curated

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Burkholderia puraquae strain LMG 29660

Burkholderia puraquae strain LMG 29660

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