Name: Azospirillum brasilense Sp 7
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 13972

Type strain:

Species: Azospirillum brasilense

Strain Designation: Sp 7

Culture col. no.: DSM 1690, ATCC 29145, JCM 1224, BCRC 12270, CCM 3862, CECT 590, IAM 12400, ICMP 8671, LMG 1263, LMG 13127, NBRC 102289, NCIMB 11860, NRRL B-14647, VKM B-1547

Strain history: ATCC 29145 <-- J. Döbereiner Sp 7.

NCBI tax ID(s): 192 (species)

SI-ID 7462

LMG 13127, ATCC 29145, CECT 590, DSM 1690, JCM 1224, LMG 1263, NCIB 11860, CCM 3862, CCRC 12270, NCIMB 11860, IAM12400, IMET 11303, NRRL B-14647, BCRC 12270, ICMP 8671, CDBB 22, CDBB 1229, VKM B-1547, NBRC 102289

Special Collections

DSMZ
JCM

Other strains from Azospirillum brasilense

Section

Azospirillum brasilense Sp 7 is a mesophilic, Gram-negative bacterium that was isolated from Digitaria decumbens roots.

Gram-negative
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Rhodospirillales*
	Family	*Azospirillaceae*
	Genus	*Azospirillum*
	Species	**Azospirillum brasilense**
	Full Scientific Name (LPSN)	Azospirillum brasilense corrig. Tarrand et al. 1979 (Approved Lists 1980)

	Synonym	Roseomonas fauriae
	Synonym	Azospirillum brasiliense

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %98.333

Information on culture and growth conditions Culture and growth conditions

[Ref.: #862]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #862]

Culture medium growth

[Ref.: #862]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #862]

Culture medium

SSE/HD1:10 (DSMZ Medium 1426)

[Ref.: #862]

Culture medium growth

[Ref.: #862]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #862]

Culture medium

HETEROTROPHIC MEDIUM H3P (DSMZ Medium 428)

[Ref.: #862]

Culture medium growth

[Ref.: #862]

Culture medium link

Medium recipe at MediaDive

[Ref.: #862]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #862]	growth	28 °C
[Ref.: #862]	growth	30 °C
[Ref.: #67770]	growth	30 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]

Ability of spore formation

no Confidence in %100

[Ref.: #862]

Name of produced compound

nitrogenase

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #862]	Sample type/isolated from	Digitaria decumbens roots
[Ref.: #862]	Host species	Digitaria decumbens
[Ref.: #862]	Country	Brazil
[Ref.: #862]	Country ISO 3 Code	BRA
[Ref.: #862]	Continent	Middle and South America

[Ref.: #67770]	Sample type/isolated from	Digitaria decumbens roots
[Ref.: #67770]	Host species	Digitaria decumbens
[Ref.: #67770]	Geographic location (country and/or sea, region)	Rio de Janeiro
[Ref.: #67770]	Country	Brazil
[Ref.: #67770]	Country ISO 3 Code	BRA
[Ref.: #67770]	Continent	Middle and South America
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root (Rhizome)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #862]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Azospirillum brasilense strain ATCC 29145 16S ribosomal RNA gene, partial sequence	AY324110	1442	GenBank ENA	192 tax ID	*
[Ref.: #20218]	Azospirillum brasilense strain DSM 1690 16S ribosomal RNA gene, partial sequence	GU256438	1400	GenBank ENA	192 tax ID	*
[Ref.: #20218]	Azospirillum brasilense gene for 16S rRNA, partial sequence, strain: NBRC 102289	AB681745	1265	GenBank ENA	192 tax ID	*
[Ref.: #20218]	A.brasilense (NCIMB 11860) 16S ribosomal RNA	Z29617	1461	GenBank ENA	192 tax ID	*
[Ref.: #20218]	A.brasilense (Sp7) 16S rRNA gene	X79739	1403	GenBank ENA	192 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Azospirillum brasilense Sp 7	GCA_002027385	contig	GenBank ENA	192 tax ID
[Ref.: #66792]	Azospirillum brasilense Sp 7	GCA_008274945	complete	GenBank ENA	192 tax ID	*
[Ref.: #66792]	Azospirillum brasilense strain Sp 7 strain Sp7	192.77	plasmid	PATRIC	192 tax ID	*
[Ref.: #66792]	Azospirillum brasilense strain Sp 7 strain Sp7	192.75	plasmid	PATRIC	192 tax ID	*
[Ref.: #66792]	Azospirillum brasilense strain Sp 7 strain Sp7	192.74	plasmid	PATRIC	192 tax ID	*
[Ref.: #66792]	Azospirillum brasilense strain Sp7	192.50	complete	PATRIC	192 tax ID	*

[Ref.: #862]	GC-content	70.0 mol%
[Ref.: #862]	GC-content	67.5 mol%
[Ref.: #67770]	GC-content	67.5 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	70 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Azospirillum sp. TSO5 PATRIC: 716760.3
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Azospirillum brasilense strain Sp7 PATRIC: 192.50
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	98.333	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	84.14	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	82.468	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	72.228	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	96.229	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	80.894	no

Availability in culture collections External links

[Ref.: #862]

Culture collection no.

DSM 1690, ATCC 29145, JCM 1224, BCRC 12270, CCM 3862, CECT 590, IAM 12400, ICMP 8671, LMG 1263, LMG 13127, NBRC 102289, NCIMB 11860, NRRL B-14647, VKM B-1547

[Ref.: #83143]

SI-ID 7462

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Characterization of a Novel cis-3-Hydroxy-l-Proline Dehydratase and a trans-3-Hydroxy-l-Proline Dehydratase from Bacteria.	Watanabe S, Fukumori F, Miyazaki M, Tagami S, Watanabe Y	J Bacteriol	10.1128/JB.00255-17	2017	*
Phylogeny	Azospirillum fermentarium sp. nov., a nitrogen-fixing species isolated from a fermenter.	Lin SY, Liu YC, Hameed A, Hsu YH, Lai WA, Shen FT, Young CC	Int J Syst Evol Microbiol	10.1099/ijs.0.050872-0	2013	*
Phylogeny	Azospirillum formosense sp. nov., a diazotroph from agricultural soil.	Lin SY, Shen FT, Young LS, Zhu ZL, Chen WM, Young CC	Int J Syst Evol Microbiol	10.1099/ijs.0.030585-0	2011	*
Phylogeny	Molecular and physiological comparison of Azospirillum spp. isolated from Rhizoctonia solani mycelia, wheat rhizosphere, and human skin wounds.	Cohen MF, Han XY, Mazzola M	Can J Microbiol	10.1139/w04-007	2004	*
Pathogenicity	[Effect of Azospirillum brasilense lectin on the kinetics of lymph nodes cell populations and cytokine status in experimental animals].	Nikitina VE, Bugaeva IO, Ponomareva EG, Tikhomirova EI, Bogomolova NV	Zh Mikrobiol Epidemiol Immunobiol		2002	*
Pathogenicity	Osmoregulation in Azospirillum brasilense: glycine betaine transport enhances growth and nitrogen fixation under salt stress.	Riou N, Le Rudulier D	J Gen Microbiol	10.1099/00221287-136-8-1455	1990	*
Enzymology	Cloning and expression in Escherichia coli of the Azospirillum brasilense Sp7 gene encoding ampicillin resistance.	Verreth C, Cammue B, Swinnen P, Crombez D, Michielsen A, Michiels K, Van Gool A, Vanderleyden J	Appl Environ Microbiol	10.1128/aem.55.8.2056-2060.1989	1989	*
Phylogeny	Isolation and characterization of Azospirillum brasilense loci that correct Rhizobium meliloti exoB and exoC mutations.	Michiels KW, Vanderleyden J, Van Gool AP, Signer ER	J Bacteriol	10.1128/jb.170.11.5401-5404.1988	1988	*
Cultivation	Cyst production and brown pigment formation in aging cultures of Azospirillum brasilense ATCC 29145.	Sadasivan L, Neyra CA	J Bacteriol	10.1128/jb.169.4.1670-1677.1987	1987	*
Enzymology	Cloning and characterization of the glnA gene of Azospirillum brasilense Sp7.	Bozouklian H, Fogher C, Elmerich C	Ann Inst Pasteur Microbiol (1985)	10.1016/s0769-2609(86)80089-8	1986	*
Metabolism	Comparison of Two Cellulomonas Strains and Their Interaction with Azospirillum brasilense in Degradation of Wheat Straw and Associated Nitrogen Fixation.	Halsall DM, Gibson AH	Appl Environ Microbiol	10.1128/aem.51.4.855-861.1986	1986	*
Phylogeny	Flocculation in Azospirillum brasilense and Azospirillum lipoferum: exopolysaccharides and cyst formation.	Sadasivan L, Neyra CA	J Bacteriol	10.1128/jb.163.2.716-723.1985	1985	*
Metabolism	Catabolism of carbohydrates and organic acids and expression of nitrogenase by azospirilla.	Martinez-Drets G, Del Gallo M, Burpee C, Burris RH	J Bacteriol	10.1128/jb.159.1.80-85.1984	1984	*
Metabolism	Intermediary carbon metabolism of Azospirillum brasilense.	Loh WH, Randles CI, Sharp WR, Miller RH	J Bacteriol	10.1128/jb.158.1.264-268.1984	1984	*
Enzymology	Enhancement of specific nitrogenase activity in Azospirillum brasilense and Klebsiella pneumoniae, inhibition in Rhizobium japonicum under air by phenol.	Werner D, Krotzky A, Berggold R, Thierfelder H, Preiss M	Arch Microbiol	10.1007/BF00690817	1982	*
Metabolism	Physiological properties and plasmid content of several strains of Azospirillum brasilense and A. lipoferum.	Franche C, Elmerich C	Ann Microbiol (Paris)		1981	*
Phylogeny	A taxonomic study of the Spirillum lipoferum group, with descriptions of a new genus, Azospirillum gen. nov. and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov.	Tarrand JJ, Krieg NR, Dobereiner J	Can J Microbiol	10.1139/m78-160	1978	*
	Chemotaxis of azospirillum species to aromatic compounds.	Lopez-de-Victoria G, Lovell CR	Appl Environ Microbiol	10.1128/aem.59.9.2951-2955.1993	1993	*
	Application of the Indirect Immunoperoxidase Stain Technique to the Flagella of Azospirillum brasilense.	Hall PG, Krieg NR	Appl Environ Microbiol	10.1128/aem.47.2.433-435.1984	1984	*
	Enhanced Mineral Uptake by Zea mays and Sorghum bicolor Roots Inoculated with Azospirillum brasilense.	Lin W, Okon Y, Hardy RW	Appl Environ Microbiol	10.1128/aem.45.6.1775-1779.1983	1983	*
	Association of azospirillum with grass roots.	Umali-Garcia M, Hubbell DH, Gaskins MH, Dazzo FB	Appl Environ Microbiol	10.1128/aem.39.1.219-226.1980	1980	*

References

#862	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 1690

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#83143

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID7462.1 )

* These data were automatically processed and therefore are not curated

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Azospirillum brasilense strain Sp7

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