Acinetobacter lwoffii (CIP 70.31, ATCC 9957, CCUG 61662)

Name: Acinetobacter lwoffii (CIP 70.31, ATCC 9957, CCUG 61662)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 139606

Type strain

Culture col. no. CIP 70.31 ATCC 9957 CCUG 61662 LMG 985 CNCTC Mi 3/65

NCBI tax ID(s) 1311804 28090

Special Collections CCUG CIP

Links

Acinetobacter lwoffii CIP 70.31 is an aerobe, mesophilic, Gram-negative prokaryote of the family Moraxellaceae.

Gram-negative rod-shaped aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 264

LMG 985,ATCC 9957,CIP 70.31,LMD 86.8,LMD 83.24,NCCB 86008,CNCTC 6172,CCUG 61662

@ref 20215

Last LPSN update 2025-12-16 10:03:22

Domain Pseudomonadati

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Pseudomonadales

Family Moraxellaceae

Genus Acinetobacter

Species Acinetobacter lwoffii

Full scientific name Acinetobacter lwoffii (Audureau 1940) Brisou and Prévot 1954 (Approved Lists 1980)

Synonyms (2)

"Moraxella lwoffi"
"Acinetobacter mesopotamicus"

BacDive ID	Other strains from **Acinetobacter lwoffii** (51)	Type strain
8058	A. lwoffii DSM 2403, ATCC 15309, CIP 644.10, JCM 6840, ... (type strain)
130854	A. lwoffii 2A, DSM 101862
8059	A. lwoffii ES1-117-3, DSM 30502
8060	A. lwoffii ES1-189-5, DSM 30543
8061	A. lwoffii ES1-189-6, DSM 30544
8062	A. lwoffii ES1-189-7, DSM 30545
8063	A. lwoffii ES1-230-1c, DSM 30554
8064	A. lwoffii ES1-306b, DSM 30571
8065	A. lwoffii ES2-W2a2, DSM 30617
8066	A. lwoffii ES-12, DSM 30754
131596	A. lwoffii Stev48, DSM 103056
23127	A. lwoffii alk 3PO c), DSM 30932
23128	A. lwoffii alk 4PO c), DSM 30935
100220	A. lwoffii STI05065(IMET), Tb2,
134719	A. lwoffii CIP 110447, NIPH 478
134901	A. lwoffii 705-86, CIP 102725
135761	A. lwoffii 2, CIP 55.102
135764	A. lwoffii CIP 55.113
137338	A. lwoffii CIP 70.20, ATCC 17985, NCDC KC 764
137455	A. lwoffii CIP 55.112
137771	A. lwoffii CIP 101718
138338	A. lwoffii 11.976, CIP 54.98
138411	A. lwoffii CIP 100598
138819	A. lwoffii CIP 110448, NIPH 715
139049	A. lwoffii CIP 70.36, ATCC 17987, NCTC 5867
139604	A. lwoffii CIP 102136
139605	A. lwoffii CIP 64.7, ATCC 17910, NCTC 10309
139612	A. lwoffii CIP 101966
141194	A. lwoffii CCUG 156, ATCC 14291
146826	A. lwoffii CCUG 32185
146914	A. lwoffii CCUG 32443
147799	A. lwoffii CCUG 34253
148628	A. lwoffii CCUG 35742 B
149260	A. lwoffii CCUG 37364
155119	A. lwoffii CCUG 56521
155467	A. lwoffii CCUG 57819
155496	A. lwoffii CCUG 57888
155617	A. lwoffii CCUG 58423
155925	A. lwoffii CCUG 59661
155983	A. lwoffii CCUG 59881
156001	A. lwoffii CCUG 59975
156039	A. lwoffii CCUG 60151
156087	A. lwoffii CCUG 60343
156142	A. lwoffii CCUG 60534
156460	A. lwoffii CCUG 61917
158827	A. lwoffii S2/2 B, DSM 110883
158828	A. lwoffii S 58/4, DSM 110938
163942	A. lwoffii GC2, JCM 31073, DSM 26953
173156	A. lwoffii CIP 70.17, ATCC 17925, LMG 1004, NCIMB 9020
173157	A. lwoffii CIP 110967, CIP 200119
173158	A. lwoffii CIP 110969, CIP 200013

Morphology

Cell morphology

@ref	Gram stain	Confidence
125438	negative	98.75
42133	negative

Colony morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Composition
42133	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
42133	CIP Medium 72

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
42133	positive	growth	30	mesophilic
42133	negative	growth	10
42133	negative	growth	37
42133	negative	growth	41
42133	negative	growth	45
62682	positive	growth	30	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
62682	aerobe
42133	obligate aerobe
125438	aerobe
125439	aerobe

Spore formation

@ref	Spore formation	Confidence
125438		94.653
125439		94.5

Halophily

@ref	Salt	Growth	Tested relation	Concentration
42133	NaCl	positive	growth	0-2 %
42133	NaCl		growth	4 %
42133	NaCl		growth	6 %
42133	NaCl		growth	8 %
42133	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
42133	16947 ChEBI	citrate	-	carbon source
68369	17634 ChEBI	D-glucose	-	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	-	assimilation	from API 20NE
42133	17234 ChEBI	glucose	-	degradation
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
42133	17632 ChEBI	nitrate	-	reduction
42133	17632 ChEBI	nitrate	-	respiration
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
42133	16301 ChEBI	nitrite	-	reduction
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Antibiotic resistance

@ref	Metabolite	Is antibiotic	Is sensitive	Is resistant
42133	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
42133	15688 ChEBI	acetoin	-
42133	17234 ChEBI	glucose
68369	35581 ChEBI	indole		from API 20NE

Enzymes

@ref	Value	Activity	Ec
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68382	beta-galactosidase	-	3.2.1.23	from API zym
42133	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
68382	beta-glucuronidase	-	3.2.1.31	from API zym
42133	catalase	+	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
68369	cytochrome oxidase	-	1.9.3.1	from API 20NE
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
42133	gamma-glutamyltransferase	-	2.3.2.2
42133	gelatinase	-
68369	gelatinase	-		from API 20NE
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
42133	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
42133	urease	+	3.5.1.5
68369	urease	-	3.5.1.5	from API 20NE
68382	valine arylamidase	-		from API zym

Fatty acid profile

Metadata FA analysis

@ref

62682

Fatty acid	Percentage	ECL
C16:1 ω7c	51	15.819
C12:0	12.5	12
C18:1 ω9c	12.4	17.769
C16:0	10.9	16
C12:0 3OH	6.4	13.455
C18:1 ω7c /12t/9t	2.6	17.824
C18:2 ω6,9c/C18:0 ANTE	1.4	17.724
C14:0	1.1	14
C14:0 3OH/C16:1 ISO I	0.8	15.485
C10:0	0.5	10
unknown 12.486	0.5	12.486

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
62682	-	-	+	+	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-
42133	-	+	+	+	-	+	-	+	-	-	+	+	-	-	-	-	-	-	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
62682	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+	-	+	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
42133	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	+	-	-	-	-	-	+	-	+	+	-	-	+	+	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	+	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Infection	#Disease
#Host	#Human
#Host	#Microbial

Isolation

@ref	Country	Country ISO 3 Code	Continent
42133	Italy	ITA	Europe
62682	Italy	ITA	Europe
42133	Italy	ITA	Europe

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
42133	1	Risk group (French classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	Acin_lwof_CIP_70_31_V1 assembly for Acinetobacter lwoffii ATCC 9957 = CIP 70.31	scaffold	GCA_000369125	1217667.3	2534682025	1311804	61.92

Genome browser: GCA_000369125

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
124043	Acinetobacter genomosp. 9 strain LMG 985 16S-23S ribosomal RNA intergenic spacer, complete sequence.	AY601836	701		28090

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Acinetobacter lwoffii ATCC 9957 = CIP 70.31
NCBI: GCA_000369125

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	94.50	no
125439	motility	BacteriaNetⓘ	no	51.90	no
125439	gram_stain	BacteriaNetⓘ	negative	72.10	no
125439	oxygen_tolerance	BacteriaNetⓘ	aerobe	91.80	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Acinetobacter lwoffii ATCC 9957 = CIP 70.31 CIP 70.31
NCBI: GCA_000369125.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	98.75	no
125438	anaerobic	anaerobicⓘ	no	97.79	yes
125438	aerobic	aerobicⓘ	yes	90.45	yes
125438	spore-forming	spore-formingⓘ	no	94.65	no
125438	thermophilic	thermophileⓘ	no	99.48	yes
125438	flagellated	motile2+ⓘ	no	80.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Genome Analysis of Acinetobacter lwoffii Strains Isolated from Permafrost Soils Aged from 15 Thousand to 1.8 Million Years Revealed Their Close Relationships with Present-Day Environmental and Clinical Isolates.	Rakitin AL, Ermakova AY, Beletsky AV, Petrova M, Mardanov AV, Ravin NV.	Biology (Basel)	10.3390/biology10090871	2021
	Analysis of Phenol Biodegradation in Antibiotic and Heavy Metal Resistant Acinetobacter lwoffii NL1.	Xu N, Qiu C, Yang Q, Zhang Y, Wang M, Ye C, Guo M.	Front Microbiol	10.3389/fmicb.2021.725755	2021
Pathogenicity	Resistance of Permafrost and Modern Acinetobacter lwoffii Strains to Heavy Metals and Arsenic Revealed by Genome Analysis.	Mindlin S, Petrenko A, Kurakov A, Beletsky A, Mardanov A, Petrova M.	Biomed Res Int	10.1155/2016/3970831	2016
Phylogeny	Identification of 50 class D beta-lactamases and 65 Acinetobacter-derived cephalosporinases in Acinetobacter spp.	Perichon B, Goussard S, Walewski V, Krizova L, Cerqueira G, Murphy C, Feldgarden M, Wortman J, Clermont D, Nemec A, Courvalin P.	Antimicrob Agents Chemother	10.1128/aac.01261-13	2014
Pathogenicity	AB569, a non-toxic combination of acidified nitrite and EDTA, is effective at killing the notorious Iraq/Afghanistan combat wound pathogens, multi-drug resistant Acinetobacter baumannii and Acinetobacter spp.	Bogue AL, Panmanee W, McDaniel CT, Mortensen JE, Kamau E, Actis LA, Johannigman JA, Schurr MJ, Satish L, Kotagiri N, Hassett DJ.	PLoS One	10.1371/journal.pone.0247513	2021
	Diverse responses to UV light exposure in Acinetobacter include the capacity for DNA damage-induced mutagenesis in the opportunistic pathogens Acinetobacter baumannii and Acinetobacter ursingii.	Hare JM, Bradley JA, Lin CL, Elam TJ.	Microbiology (Reading)	10.1099/mic.0.054668-0	2012
Phylogeny	Species, biotype, and bacteriophage type determinations compared with cell envelope protein profiles for typing Acinetobacter strains.	Bouvet PJ, Jeanjean S, Vieu JF, Dijkshoorn L.	J Clin Microbiol	10.1128/jcm.28.2.170-176.1990	1990
Phylogeny	Cultural and chemical characterization of CDC groups EO-2, M-5, and M-6, Moraxella (Moraxella) species, Oligella urethralis, Acinetobacter species, and Psychrobacter immobilis.	Moss CW, Wallace PL, Hollis DG, Weaver RE.	J Clin Microbiol	10.1128/jcm.26.3.484-492.1988	1988
Phylogeny	Interspecies transformation of Acinetobacter: genetic evidence for a ubiquitous genus.	Juni E.	J Bacteriol	10.1128/jb.112.2.917-931.1972	1972
	Acinetobacter spp. as nosocomial pathogens: microbiological, clinical, and epidemiological features.	Bergogne-Berezin E, Towner KJ.	Clin Microbiol Rev	10.1128/cmr.9.2.148	1996
Pathogenicity	Bioinformatic Analysis of the Type VI Secretion System and Its Potential Toxins in the Acinetobacter Genus.	Repizo GD, Espariz M, Seravalle JL, Salcedo SP.	Front Microbiol	10.3389/fmicb.2019.02519	2019
Enzymology	Genes encoding OXA-134-like enzymes are found in Acinetobacter lwoffii and A. schindleri and can be used for identification.	Turton JF, Hyde R, Martin K, Shah J	J Clin Microbiol	10.1128/JCM.06173-11	2012

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#42133	Collection of Institut Pasteur ; Curators of the CIP; CIP 70.31
#62682	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 61662
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Acinetobacter lwoffii (CIP 70.31, ATCC 9957, CCUG 61662)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

Fatty acid profile?

API zym

API 20NE

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000369125

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media

Fatty acid profile