Inquilinus limosus (CIP 108342, CCM 7171, DSM 16000)

Name: Inquilinus limosus (CIP 108342, CCM 7171, DSM 16000)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13941

Type strain

Strain Designation AU476

Culture col. no. CIP 108342 CCM 7171 DSM 16000 CCUG 45653 LMG 20952 1 more

NCBI tax ID(s) 1122125 171674

Special Collections DSMZ CCUG CIP

History <- CCUG <- LMG <- P. Vandamme <- T. Coenye CIP <- 2004, CCUG <- 2002, LMG <- P. Vandamme, Gent, Belgium <- T. Coenye, Michigan, USA: strain AU476

Links

Inquilinus limosus AU476 is an aerobe, Gram-negative, rod-shaped bacterium that was isolated from sputum of cystic fibrosis patient.

Gram-negative rod-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 84281

LMG 20952,CCUG 45653,DSM 16000,CCM 7171,CIP 108342

@ref 20215

Last LPSN update 2026-02-09 11:21:16

Domain Bacteria

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Rhodospirillales

Family Azospirillaceae

Genus Inquilinus

Species Inquilinus limosus

Full scientific name Inquilinus limosus Coenye et al. 2002

BacDive ID	Other strains from **Inquilinus limosus** (4)	Type strain
139881	I. limosus 579896, 103798, DSM 103798
152055	I. limosus CCUG 45650
152058	I. limosus CCUG 45656
152185	I. limosus CCUG 45997

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
120606	negative	rod-shaped
125438	negative		95.5
125439	negative		90.6

Colony morphology

Multimedia

@ref:	66793
multimedia content:	EM_DSM_16000_1.jpg
multimedia.multimedia content:	EM_DSM_16000_1.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_16000_2.jpg
multimedia.multimedia content:	EM_DSM_16000_2.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_16000_3.jpg
multimedia.multimedia content:	EM_DSM_16000_3.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_16000_4.jpg
multimedia.multimedia content:	EM_DSM_16000_4.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_16000_5.jpg
multimedia.multimedia content:	EM_DSM_16000_5.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
6233	CASO AGAR (MERCK 105458) (DSMZ Medium 220)	Medium recipe at MediaDive	Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
6233	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
6233	TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545)	Medium recipe at MediaDive	Name: TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545) Composition: Casein peptone 17.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l D(+)-Glucose 2.5 g/l K2HPO4 2.5 g/l Distilled water
36722	MEDIUM 328- for nutrient agar		Distilled water make up to (1000.000 ml);Agar (15.000 g);Peptone (5.000g);Beef extract (3.000 g)
120606	CIP Medium 328	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
6233	positive	growth	30
6233	positive	growth	28
36722	positive	growth	37
56864	positive	growth	37-42
120606	positive	growth	30-41
120606	negative	growth	5
120606	negative	growth	10

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
56864	aerobe
120606	obligate aerobe
125439	obligate aerobe	98.5

Halophily

@ref	Salt	Growth	Tested relation	Concentration
120606	NaCl	positive	growth	0-4 %
120606	NaCl		growth	6 %
120606	NaCl		growth	8 %
120606	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
120606	16947 ChEBI	citrate	-	carbon source
120606	4853 ChEBI	esculin	+	hydrolysis
120606	29864 ChEBI	mannitol	-	fermentation
120606	17632 ChEBI	nitrate	-	reduction
120606	16301 ChEBI	nitrite	-	reduction

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
120606	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
120606	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
120606	15688 ChEBI	acetoin	-
120606	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
120606	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
120606	amylase	-
68382	beta-galactosidase	+	3.2.1.23	from API zym
120606	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
120606	caseinase	-	3.4.21.50
120606	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
120606	DNase	+
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
120606	gelatinase	-
120606	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
120606	lipase	-
68382	lipase (C 14)	-		from API zym
120606	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
120606	ornithine decarboxylase	-	4.1.1.17
120606	oxidase	+
120606	phenylalanine ammonia-lyase	-	4.3.1.24
68382	trypsin	+	3.4.21.4	from API zym
120606	tryptophan deaminase	-
120606	tween esterase	-
120606	urease	-	3.5.1.5
68382	valine arylamidase	+		from API zym

Fatty acid profile

Metadata FA analysis

@ref

56864

Fatty acid	Percentage	ECL
C18:1 ω7c /12t/9t	82.4	17.824
C19:0 CYCLO ω8c	9.3	18.9
C18:0	3.3	18
C16:0	2.2	16
C17:0	1.6	17
C18:2 ω6,9c/C18:0 ANTE	1.2	17.724

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
120606	-	+	+	+	-	+	+	-	+	-	+	+	-	+	-	-	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
120606	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Infection	#Disease	#Cystic fibrosis
#Infection	#Patient	-
#Host Body Product	#Fluids	#Sputum

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
6233	sputum of cystic fibrosis patient	USA	USA	North America
56864	Human sputum,cystic fibrosis	USA	USA	North America
120606	Human, Sputum of systic fibrosis	United States of America	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence AY043374 (>99% sequence identity) for Inquilinus from Microbeatlas

Aquatic Samples	1537
Soil Samples	12295
Animal Samples	789
Plant Samples	2940
Total Samples	17561

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Biosafety level	Biosafety level comment
6233	yes, in single cases	1	Risk group (German classification) According to TRBA 466
120606		1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM42318v1 assembly for Inquilinus limosus DSM 16000	scaffold	GCA_000423185	1122125.13	2523533609	1122125	62.91

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
6233	Inquilinus limosus strain AU476 16S ribosomal RNA gene, partial sequence	AY043374	1418		171674

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Inquilinus limosus DSM 16000
NCBI: GCA_000423185

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	88.20	no
125439	motility	BacteriaNetⓘ	yes	65.50	no
125439	gram_stain	BacteriaNetⓘ	negative	90.60	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.50	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Inquilinus limosus DSM 16000 DSM 16000
NCBI: GCA_000423185.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	95.50	no
125438	anaerobic	anaerobicⓘ	no	95.71	yes
125438	aerobic	aerobicⓘ	yes	85.05	no
125438	spore-forming	spore-formingⓘ	no	80.93	no
125438	thermophilic	thermophileⓘ	no	96.79	yes
125438	flagellated	motile2+ⓘ	yes	61.46	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Current knowledge on Inquilinus limosus, a scarcely researched human pathogen.	Akinnurun OM, Riedel T, Muller S, Bunk B, Schrottner P.	BMC Microbiol	10.1186/s12866-024-03617-6	2024
	Inquilinus Species Infections in Humans-A Narrative Review.	Vasilopoulou A, Panayiotou T, Baliou S, Tsantes AG, Ioannou P.	Microorganisms	10.3390/microorganisms13030592	2025
Enzymology	Bacterial Genome Mining of Enzymatic Tools for Alkyne Biosynthesis.	Zhu X, Su M, Manickam K, Zhang W.	ACS Chem Biol	10.1021/acschembio.5b00641	2015
Metabolism	Arsinothricin, an arsenic-containing non-proteinogenic amino acid analog of glutamate, is a broad-spectrum antibiotic.	Nadar VS, Chen J, Dheeman DS, Galvan AE, Yoshinaga-Sakurai K, Kandavelu P, Sankaran B, Kuramata M, Ishikawa S, Rosen BP, Yoshinaga M, Yoshinaga M.	Commun Biol	10.1038/s42003-019-0365-y	2019
Enzymology	Bacterial Enzymes Catalyzing the Synthesis of 1,8-Dihydroxynaphthalene, a Key Precursor of Dihydroxynaphthalene Melanin, from Sorangium cellulosum.	Sone Y, Nakamura S, Sasaki M, Hasebe F, Kim SY, Funa N.	Appl Environ Microbiol	10.1128/aem.00258-18	2018
Phylogeny	An updated phylogeny of the Alphaproteobacteria reveals that the parasitic Rickettsiales and Holosporales have independent origins.	Munoz-Gomez SA, Hess S, Burger G, Lang BF, Susko E, Slamovits CH, Roger AJ.	Elife	10.7554/elife.42535	2019
Phylogeny	An integrated phylogenomic approach toward pinpointing the origin of mitochondria.	Wang Z, Wu M.	Sci Rep	10.1038/srep07949	2015
Phylogeny	Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria.	Mellmann A, Cloud J, Maier T, Keckevoet U, Ramminger I, Iwen P, Dunn J, Hall G, Wilson D, Lasala P, Kostrzewa M, Harmsen D.	J Clin Microbiol	10.1128/jcm.00157-08	2008
Enzymology	Inquilinus limosus in patients with cystic fibrosis, Germany.	Wellinghausen N, Essig A, Sommerburg O.	Emerg Infect Dis	10.3201/eid1103.041078	2005
Pathogenicity	Exopolysaccharides produced by Inquilinus limosus, a new pathogen of cystic fibrosis patients: novel structures with usual components.	Herasimenka Y, Cescutti P, Impallomeni G, Rizzo R	Carbohydr Res	10.1016/j.carres.2007.07.012	2007
Phylogeny	Inquilinus ginsengisoli sp. nov., isolated from soil of a ginseng field.	Jung HM, Lee JS, Bae HM, Yi TH, Kim SY, Lee ST, Im WT	Int J Syst Evol Microbiol	10.1099/ijs.0.018689-0	2010

References

#6233	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16000
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#36722	; Curators of the CIP;
#56864	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 45653
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66793	Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120606	Collection of Institut Pasteur ; Curators of the CIP; CIP 108342
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Inquilinus limosus (CIP 108342, CCM 7171, DSM 16000)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

Fatty acid profile

API zym

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000423185

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media