Inquilinus limosus AU476 | Type strain | CIP 108342, CCM 7171, DSM 16000, CCUG 45653, LMG 20952 | BacDiveID:13941

Strain identifier

BacDive ID: 13941

Type strain:

Species: Inquilinus limosus

Strain Designation: AU476

Culture col. no.: CIP 108342, CCM 7171, DSM 16000, CCUG 45653, LMG 20952

Strain history: CIP <- 2004, CCUG <- 2002, LMG <- P. Vandamme, Gent, Belgium <- T. Coenye, Michigan, USA: strain AU476

NCBI tax ID(s): 1122125 (strain), 171674 (species)

SI-ID 84281

LMG 20952, CCM 7171, CIP 108342, CCUG 45653, DSM 16000

Special Collections

Other strains from Inquilinus limosus

Section

When using BacDive for research please cite our paper

Inquilinus limosus AU476 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from sputum of cystic fibrosis patient.

Gram-negative
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Rhodospirillales*
	Family	*Azospirillaceae*
	Genus	*Inquilinus*
	Species	**Inquilinus limosus**
	Full Scientific Name (LPSN)	Inquilinus limosus Coenye et al. 2002

Information on morphological and physiological properties Morphology

[Ref.: #120606]	Gram stain	negative
[Ref.: #125438]	Gram stain	negative Confidence in %95.5
[Ref.: #125439]	Gram stain	negative Confidence in %90.6

[Ref.: #120606]	Cell shape	rod-shaped

Multimedia content

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Information on culture and growth conditions Culture and growth conditions

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

Culture medium growth

Culture medium link

Medium recipe at MediaDive

Culture medium composition

expand / minimize

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

Culture medium growth

Culture medium link

Medium recipe provided by DSMZ

Culture medium

TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545)

Culture medium growth

Culture medium link

Medium recipe provided by DSMZ

Culture medium

MEDIUM 328- for nutrient agar

Culture medium growth

Culture medium composition

expand / minimize

[Ref.: #120606]

Culture medium

CIP Medium 328

[Ref.: #120606]

Culture medium growth

[Ref.: #120606]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #6233]			growth	30 °C
[Ref.: #6233]			growth	28 °C
[Ref.: #36722]			growth	37 °C
[Ref.: #56864]			growth	37-42 °C
[Ref.: #120606]			growth	30-41 °C
[Ref.: #120606]		no	growth	5 °C
[Ref.: #120606]		no	growth	10 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #56864]	Oxygen tolerance	aerobe
[Ref.: #120606]	Oxygen tolerance	obligate aerobe
[Ref.: #125439]	Oxygen tolerance	obligate aerobe Confidence in %98.5

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #120606]		NaCl		growth	0-4	%
[Ref.: #120606]		NaCl	no	growth	6	%
[Ref.: #120606]		NaCl	no	growth	8	%
[Ref.: #120606]		NaCl	no	growth	10	%

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #120606]		citrate ChEBI	-	carbon source
[Ref.: #120606]		esculin ChEBI	+	hydrolysis
[Ref.: #120606]		mannitol ChEBI	-	fermentation
[Ref.: #120606]		nitrate ChEBI	-	reduction
[Ref.: #120606]		nitrite ChEBI	-	reduction

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #120606]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #120606]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #120606]		acetoin ChEBI	-
[Ref.: #120606]		glucose ChEBI		-

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #68382]		acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #120606]		alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]		alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]		alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]		alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]		alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]		alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]		alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #120606]		amylase	-
[Ref.: #120606]		beta-galactosidase	+	3.2.1.23
[Ref.: #68382]		beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]		beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]		beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #120606]		caseinase	-	3.4.21.50
[Ref.: #120606]		catalase	+	1.11.1.6
[Ref.: #68382]		cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #120606]		DNase	+
[Ref.: #68382]		esterase (C 4)	+		* from API zym
[Ref.: #68382]		esterase lipase (C 8)	+		* from API zym
[Ref.: #120606]		gelatinase	-
[Ref.: #120606]		lecithinase	-
[Ref.: #68382]		leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #120606]		lipase	-
[Ref.: #68382]		lipase (C 14)	-		* from API zym
[Ref.: #120606]		lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]		N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]		naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #120606]		ornithine decarboxylase	-	4.1.1.17
[Ref.: #120606]		oxidase	+
[Ref.: #120606]		phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68382]		trypsin	+	3.4.21.4	* from API zym
[Ref.: #120606]		tryptophan deaminase	-
[Ref.: #120606]		tween esterase	-
[Ref.: #120606]		urease	-	3.5.1.5
[Ref.: #68382]		valine arylamidase	+		* from API zym
		Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

Fatty acid	Percentage	ECL
C18:1 ω7c /12t/9t	82.4	17.824
C19:0 CYCLO ω8c	9.3	18.9
C18:0	3.3	18
C16:0	2.2	16
C17:0	1.6	17
C18:2 ω6,9c/C18:0 ANTE	1.2	17.724

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #120606]	2713	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120606]	11106	-	+	+	+	-	+	+	-	+	-	+	+	-	+	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6233]	Sample type/isolated from	sputum of cystic fibrosis patient
[Ref.: #6233]	Country	USA
[Ref.: #6233]	Country ISO 3 Code	USA
[Ref.: #6233]	Continent	North America

[Ref.: #56864]	Sample type/isolated from	Human sputum,cystic fibrosis
[Ref.: #56864]	Country	USA
[Ref.: #56864]	Country ISO 3 Code	USA
[Ref.: #56864]	Continent	North America

[Ref.: #120606]	Sample type/isolated from	Human, Sputum of systic fibrosis
[Ref.: #120606]	Country	United States of America
[Ref.: #120606]	Country ISO 3 Code	USA
[Ref.: #120606]	Continent	North America
* marker position based on {}

Isolation sources categories

#Infection	#Disease	#Cystic fibrosis
#Infection	#Patient	-
#Host Body Product	#Fluids	#Sputum

What are isolation sources categories?

Global distribution of 16S sequence AY043374 (>99% sequence identity) for Inquilinus from Microbeatlas

Aquatic Samples	1537
Soil Samples	12295
Animal Samples	789
Plant Samples	2940
Total Samples	17561

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6233]	Pathogenicity (human)	yes, in single cases
[Ref.: #6233]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120606]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6233]	Inquilinus limosus strain AU476 16S ribosomal RNA gene, partial sequence	AY043374	1418	GenBank ENA	171674 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Inquilinus limosus DSM 16000	GCA_000423185	scaffold	GenBank ENA	1122125 tax ID	*
[Ref.: #66792]	Inquilinus limosus DSM 16000	1122125.13	wgs	PATRIC	1122125 tax ID	*
[Ref.: #66792]	Inquilinus limosus DSM 16000	2523533609	draft	JGI IMG/M	1122125 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Model	Trait	Prediction	Confidence in %	In training data
	Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Inquilinus limosus DSM 16000 DSM 16000 NCBI: GCA_000423185.1
[Ref.: #125438]	gram-positive	gram-positiveⓘ	no	95.5	no
[Ref.: #125438]	anaerobic	anaerobicⓘ	no	95.714	yes
[Ref.: #125438]	aerobic	aerobicⓘ	yes	85.052	no
[Ref.: #125438]	spore-forming	spore-formingⓘ	no	80.925	no
[Ref.: #125438]	thermophile	thermophilicⓘ	no	96.786	yes
[Ref.: #125438]	motile2+	flagellatedⓘ	yes	61.462	no

	Model	Trait	Prediction	Confidence in %
	Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Inquilinus limosus DSM 16000 NCBI: GCA_000423185
[Ref.: #125439]	BacteriaNet	spore_formationⓘ	no	88.2
[Ref.: #125439]	BacteriaNet	motilityⓘ	yes	65.5
[Ref.: #125439]	BacteriaNet	gram_stainⓘ	negative	90.6
[Ref.: #125439]	BacteriaNet	oxygen_toleranceⓘ	obligate aerobe	98.5

Availability in culture collections External links

Culture collection no.

CIP 108342, CCM 7171, DSM 16000, CCUG 45653, LMG 20952

SI-ID 84281

*

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Inquilinus ginsengisoli sp. nov., isolated from soil of a ginseng field.	Jung HM, Lee JS, Bae HM, Yi TH, Kim SY, Lee ST, Im WT	Int J Syst Evol Microbiol	10.1099/ijs.0.018689-0	2010	*
Pathogenicity	Exopolysaccharides produced by Inquilinus limosus, a new pathogen of cystic fibrosis patients: novel structures with usual components.	Herasimenka Y, Cescutti P, Impallomeni G, Rizzo R	Carbohydr Res	10.1016/j.carres.2007.07.012	2007	*

References

#6233

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16000

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#36722 ; Curators of the CIP;
#56864 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 45653

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66793

Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#83114

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID84281.1 )

#120606 Collection of Institut Pasteur ; Curators of the CIP; CIP 108342

#125438

Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )

#125439

Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.