Name: Lactobacillus crispatus CIP 105002
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 139287

Type strain:

Species: Lactobacillus crispatus

Culture col. no.: CIP 105002, JCM 5810, KCTC 3178, LMG 18191

Strain history: CIP <- 1996, JCM <- E. Lauer <- T. Mitsuoka: strain T-91, Lactobacillus acidophilus

NCBI tax ID(s): 47770 (species)

SI-ID 11442

LMG 18191, JCM 5810, KCTC 3178, CIP 105002

Other strains from Lactobacillus crispatus

Section

Lactobacillus crispatus CIP 105002 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from Chicken feces.

Gram-positive
facultative anaerobe
mesophilic
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Lactobacillus*
	Species	**Lactobacillus crispatus**
	Full Scientific Name (LPSN)	Lactobacillus crispatus (Brygoo and Aladame 1953) Moore and Holdeman 1970 (Approved Lists 1980)

Synonym

"Eubacterium crispatum"

Information on morphological and physiological properties Morphology

[Ref.: #41426]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #41426]	Cell shape	rod-shaped

[Ref.: #41426]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #41426]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #41426]

Culture medium growth

[Ref.: #41426]

Culture medium composition

expand / minimize

[Ref.: #41426]

Culture medium

CIP Medium 40

[Ref.: #41426]

Culture medium growth

[Ref.: #41426]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #41426]		growth	37 °C
[Ref.: #41426]		growth	30-45 °C
[Ref.: #41426]	no	growth	10 °C
[Ref.: #41426]	no	growth	15 °C
[Ref.: #41426]	no	growth	25 °C
[Ref.: #67770]		growth	37 °C

[Ref.: #41426]	Temperature range	mesophilic
[Ref.: #41426]	Temperature range	psychrophilic
[Ref.: #41426]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #41426]

Oxygen tolerance

facultative anaerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.997

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #41426]	nitrate ChEBI	-	reduction
[Ref.: #41426]	nitrate ChEBI	+	respiration
[Ref.: #41426]	nitrite ChEBI	-	reduction

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #41426]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #41426]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #41426]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #41426]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #41426]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #41426]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #41426]	8368	-	-	+	-	-	-	-	-	-	-	+	+	+	+	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67770]	Sample type/isolated from	Chicken feces

[Ref.: #41426]	Sample type/isolated from	Chicken, feces
* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #41426]

Biosafety level

Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Lactobacillus crispatus JCM 5810	GCA_001567095	contig	GenBank	47770 tax ID	*
[Ref.: #66792]	Lactobacillus crispatus strain JCM 5810	47770.37	wgs	PATRIC	47770 tax ID	*
[Ref.: #66792]	Lactobacillus crispatus JCM 5810	2690315764	draft	JGI IMG/M	47770 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Lactobacillus crispatus strain JCM 5810 PATRIC: 47770.37
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lactobacillus crispatus strain JCM 5810 PATRIC: 47770.37
[Ref.: #69480]	gram-positive	yes	95.932	no
[Ref.: #69480]	anaerobic	yes	57.682	no
[Ref.: #69480]	halophile	yes	82.213	no
[Ref.: #69480]	spore-forming	no	95.201	no
[Ref.: #69480]	glucose-util	yes	93.336	no
[Ref.: #69480]	aerobic	no	98.599	no
[Ref.: #69480]	flagellated	no	98.262	no
[Ref.: #69480]	motile	no	96.659	no
[Ref.: #69480]	glucose-ferment	yes	90.74	no
[Ref.: #69480]	thermophile	no	98.907	yes

Availability in culture collections External links

[Ref.: #41426]

Culture collection no.

CIP 105002, JCM 5810, KCTC 3178, LMG 18191

[Ref.: #95827]

SI-ID 11442

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Metabolism	S-layer protein 2 of Lactobacillus crispatus 2029, its structural and immunomodulatory characteristics and roles in protective potential of the whole bacteria against foodborne pathogens.	Abramov VM, Kosarev IV, Priputnevich TV, Machulin AV, Khlebnikov VS, Pchelintsev SY, Vasilenko RN, Sakulin VK, Suzina NE, Chikileva IO, Derysheva EI, Melnikov VG, Nikonov IN, Samoilenko VA, Svetoch EE, Sukhikh GT, Uversky VN, Karlyshev AV	Int J Biol Macromol	10.1016/j.ijbiomac.2020.02.065	2020	*
Genetics	Draft Genome Sequence of Lactobacillus crispatus JCM5810, Which Can Reduce Campylobacter jejuni Colonization in Chicken Intestine.	Wooten J, Liu X, Miller MJ	Genome Announc	10.1128/genomeA.00255-16	2016	*
Metabolism	Domains in the S-layer protein CbsA of Lactobacillus crispatus involved in adherence to collagens, laminin and lipoteichoic acids and in self-assembly.	Antikainen J, Anton L, Sillanpaa J, Korhonen TK	Mol Microbiol	10.1046/j.1365-2958.2002.03180.x	2002	*
Enzymology	Inhibition of the adherence of Escherichia coli strains to basement membrane by Lactobacillus crispatus expressing an S-layer.	Horie M, Ishiyama A, Fujihira-Ueki Y, Sillanpaa J, Korhonen TK, Toba T	J Appl Microbiol	10.1046/j.1365-2672.2002.01539.x	2002	*
Metabolism	Expression of cbsA encoding the collagen-binding S-protein of Lactobacillus crispatus JCM5810 in Lactobacillus casei ATCC 393(T).	Martinez B, Sillanpaa J, Smit E, Korhonen TK, Pouwels PH	J Bacteriol	10.1128/JB.182.23.6857-6861.2000	2000	*
Metabolism	Characterization of the collagen-binding S-layer protein CbsA of Lactobacillus crispatus.	Sillanpaa J, Martinez B, Antikainen J, Toba T, Kalkkinen N, Tankka S, Lounatmaa K, Keranen J, Hook M, Westerlund-Wikstrom B, Pouwels PH, Korhonen TK	J Bacteriol	10.1128/JB.182.22.6440-6450.2000	2000	*
	A Collagen-Binding S-Layer Protein in Lactobacillus crispatus.	Toba T, Virkola R, Westerlund B, Bjorkman Y, Sillanpaa J, Vartio T, Kalkkinen N, Korhonen TK	Appl Environ Microbiol	10.1128/aem.61.7.2467-2471.1995	1995	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#41426 Collection of Institut Pasteur ; Curators of the CIP; CIP 105002

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#95827

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID11442.1 )

* These data were automatically processed and therefore are not curated

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Lactobacillus crispatus strain JCM 5810

Lactobacillus crispatus strain JCM 5810

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