Name: Thauera butanivorans Bu B1211
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13921

Type strain:

Species: Thauera butanivorans

Strain Designation: Bu B1211, BUB-1211

Culture col. no.: DSM 2080, ATCC 43655, IAM 12574, CCUG 51053, JCM 20651, NBRC 103042, CIP 107886

Strain history: CIP <- 2003, IAM <- I. Koumura: strain BUB-1211

NCBI tax ID(s): 1219356 (strain), 86174 (species)

SI-ID 46846

ATCC 43655, IAM 12574, DSM 2080, JCM 20651, NBRC 103042, CIP 107886, CCUG 51053

Section

Thauera butanivorans Bu B1211 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from activated sludge, oil refining plant.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Rhodocyclales*
	Family	*Zoogloeaceae*
	Genus	*Thauera*
	Species	**Thauera butanivorans**
	Full Scientific Name (LPSN)	Thauera butanivorans (ex Takahashi et al. 1980) Dubbels et al. 2009

Synonym

"Pseudomonas butanovora"

Information on morphological and physiological properties Morphology

[Ref.: #28891]	Gram stain	negative
[Ref.: #117080]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %100

[Ref.: #28891]	Cell length	1.75 µm

[Ref.: #28891]	Cell width	0.7 µm

[Ref.: #28891]	Cell shape	rod-shaped
[Ref.: #117080]	Cell shape	rod-shaped

[Ref.: #28891]	Motility	yes
[Ref.: #117080]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %99.349

[Ref.: #117080]	Pigment production	no
[Ref.: #117080]	Pigment name	Pyocyanin

Information on culture and growth conditions Culture and growth conditions

[Ref.: #715]

Culture medium

MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457)

[Ref.: #715]

Culture medium growth

[Ref.: #715]

Culture medium link

Medium recipe at MediaDive

[Ref.: #715]

Culture medium composition

expand / minimize

[Ref.: #715]

Culture medium

REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a)

[Ref.: #715]

Culture medium growth

[Ref.: #715]

Culture medium link

Medium recipe at MediaDive

[Ref.: #715]

Culture medium composition

expand / minimize

[Ref.: #39959]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #39959]

Culture medium growth

[Ref.: #39959]

Culture medium composition

expand / minimize

[Ref.: #117080]

Culture medium

CIP Medium 72

[Ref.: #117080]

Culture medium growth

[Ref.: #117080]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #715]		growth	28 °C
[Ref.: #715]		growth	30 °C
[Ref.: #39959]		growth	30 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #117080]		growth	10-41 °C
[Ref.: #117080]	no	growth	5 °C

[Ref.: #715]	Temperature range	mesophilic
[Ref.: #39959]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #117080]	Temperature range	psychrophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #28891]	Oxygen tolerance	aerobe
[Ref.: #117080]	Oxygen tolerance	obligate aerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.984

[Ref.: #715]

Name of produced compound

protein

	Salt	Tested relation		Salt conc.
[Ref.: #117080]	NaCl		growth	0-2	%
[Ref.: #117080]	NaCl	no	growth	4	%
[Ref.: #117080]	NaCl	no	growth	6	%
[Ref.: #117080]	NaCl	no	growth	8	%
[Ref.: #117080]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #117080]	citrate ChEBI	+	carbon source
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #117080]	esculin ChEBI	-	hydrolysis
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #28891]	ethanol ChEBI	+	carbon source
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #117080]	nitrate ChEBI	+	reduction
[Ref.: #117080]	nitrate ChEBI	+	respiration
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #117080]	nitrite ChEBI	+	reduction
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #117080]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes	no

	Metabolite production	Metabolite	Production
[Ref.: #117080]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #117080]	alcohol dehydrogenase	+	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #117080]	amylase	-
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #117080]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE, API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #117080]	caseinase	-	3.4.21.50
[Ref.: #117080]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #117080]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #117080]	gelatinase	-
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #117080]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #117080]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #117080]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #117080]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #117080]	oxidase	+
[Ref.: #117080]	protease	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #117080]	tryptophan deaminase	-
[Ref.: #117080]	tween esterase	+
[Ref.: #117080]	urease	-	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #715]	24874	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+	+	+	+

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #117080]	2481	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+	+	+	-	-	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	+	+	-	-	-	-	-	-	+	+	-	+	+	+	+	+	+	+	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #117080]	10725	-	-	+	-	-	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #715]	Sample type/isolated from	activated sludge, oil refining plant
[Ref.: #715]	Country	Japan
[Ref.: #715]	Country ISO 3 Code	JPN
[Ref.: #715]	Continent	Asia

[Ref.: #59094]	Sample type/isolated from	Sludge,activated,oil refining plant
[Ref.: #59094]	Country	Japan
[Ref.: #59094]	Country ISO 3 Code	JPN
[Ref.: #59094]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Activated sludge from an oil refining plant

[Ref.: #117080]	Sample type/isolated from	Environment, Activated sludge
[Ref.: #117080]	Country	Japan
[Ref.: #117080]	Country ISO 3 Code	JPN
[Ref.: #117080]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered

#Waste

#Activated sludge

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #715]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #117080]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #715]	Thauera butanivorans gene for 16S rRNA, strain: IAM 12574	AB021377	1502	ENA	1219356 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Thauera butanivorans NBRC 103042	GCA_001591165	contig	GenBank	1219356 tax ID
[Ref.: #66792]	Thauera butanivorans NBRC 103042	1219356.5	wgs	PATRIC	1219356 tax ID	*
[Ref.: #66792]	Thauera butanivorans NBRC 103042	2731957639	draft	JGI IMG/M	1219356 tax ID	*

[Ref.: #715]	GC-content	67.3 mol%
[Ref.: #67770]	GC-content	67.3 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Thauera butanivorans NBRC 103042 PATRIC: 1219356.5
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Thauera butanivorans NBRC 103042 PATRIC: 1219356.5
[Ref.: #69480]	motile	yes	91.966	no
[Ref.: #69480]	gram-positive	no	98.621	no
[Ref.: #69480]	anaerobic	no	96.484	no
[Ref.: #69480]	aerobic	yes	82.154	yes
[Ref.: #69480]	halophile	no	90.099	no
[Ref.: #69480]	spore-forming	no	95.452	no
[Ref.: #69480]	thermophile	no	99.062	yes
[Ref.: #69480]	glucose-util	no	87.248	yes
[Ref.: #69480]	flagellated	yes	84.544	no
[Ref.: #69480]	glucose-ferment	no	89.233	yes

Availability in culture collections External links

[Ref.: #715]

Culture collection no.

DSM 2080, ATCC 43655, IAM 12574, CCUG 51053, JCM 20651, NBRC 103042, CIP 107886

[Ref.: #83095]

SI-ID 46846

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Thauera butanivorans sp. nov., a C2-C9 alkane-oxidizing bacterium previously referred to as 'Pseudomonas butanovora'.	Dubbels BL, Sayavedra-Soto LA, Bottomley PJ, Arp DJ	Int J Syst Evol Microbiol	10.1099/ijs.0.000638-0	2009	*
Metabolism	Two distinct alcohol dehydrogenases participate in butane metabolism by Pseudomonas butanovora.	Vangnai AS, Arp DJ, Sayavedra-Soto LA	J Bacteriol	10.1128/JB.184.7.1916-1924.2002	2002	*

References

#715	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2080

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#28891

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25331 (see below)

#39959 ; Curators of the CIP;
#59094 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 51053

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#83095

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID46846.1 )

#117080 Collection of Institut Pasteur ; Curators of the CIP; CIP 107886

#25331

IJSEM 1576 2009 ( DOI 10.1099/ijs.0.000638-0 , PubMed 19528200 )

* These data were automatically processed and therefore are not curated

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Thauera butanivorans NBRC 103042

Thauera butanivorans NBRC 103042

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