Name: Azospira restricta DSM 18626
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13893

Type strain:

Species: Azospira restricta

Culture col. no.: DSM 18626, CCUG 55598, NRRL B-41660, LMG 23819, Sua SUA2, Sua 2

Strain history: <- W. M. Moe; SUA2 <- H.-S. Bae

NCBI tax ID(s): 404405 (species)

SI-ID 292492

LMG 23819, NRRL B-41660, DSM 18626, CCUG 55598

Section

Azospira restricta DSM 18626 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from groundwater.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Rhodocyclales*
	Family	*Rhodocyclaceae*
	Genus	*Azospira*
	Species	**Azospira restricta**
	Full Scientific Name (LPSN)	Azospira restricta Bae et al. 2007

Information on morphological and physiological properties Morphology

[Ref.: #32100]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.998

[Ref.: #32100]	Cell length	1.85 µm

[Ref.: #32100]	Cell width	0.5 µm

[Ref.: #32100]	Cell shape	rod-shaped

[Ref.: #32100]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %98.946

[Ref.: #60664]

Incubation period

2-3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7626]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #7626]

Culture medium growth

[Ref.: #7626]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7626]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #7626]	growth	37 °C
[Ref.: #32100]	growth	20-40 °C
[Ref.: #32100]	optimum	37 °C
[Ref.: #60664]	growth	37 °C

[Ref.: #7626]	Temperature range	mesophilic
[Ref.: #32100]	Temperature range	mesophilic
[Ref.: #60664]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #32100]			optimum	7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #32100]	Oxygen tolerance	aerobe
[Ref.: #60664]	Oxygen tolerance	aerobe

[Ref.: #32100]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %97
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.991

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #32100]		NaCl		growth	0.0-2.0	%
[Ref.: #32100]		NaCl		optimum	1	%

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #32100]		putrescine ChEBI	+	carbon source

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #32100]		catalase	+	1.11.1.6
[Ref.: #32100]		cytochrome oxidase	+	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7626]	Sample type/isolated from	groundwater
[Ref.: #7626]	Geographic location (country and/or sea, region)	Louisiana, Baton Rouge
[Ref.: #7626]	Country	USA
[Ref.: #7626]	Country ISO 3 Code	USA
[Ref.: #7626]	Continent	North America

[Ref.: #60664]	Sample type/isolated from	Groundwater
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Groundwater

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence DQ974114 (>99% sequence identity) for Azospira restricta subclade from Microbeatlas

Aquatic Samples	181
Soil Samples	62
Animal Samples	8
Plant Samples	21
Total Samples	272

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7626]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7626]	Azospira restricta strain SUA2 16S ribosomal RNA gene, partial sequence	DQ974114	1482	ENA	404405 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Azospira restricta DSM 18626	GCA_016858125	complete	GenBank	404405 tax ID	*
[Ref.: #66792]	Azospira restricta strain DSM 18626	404405.4	complete	PATRIC	404405 tax ID	*

[Ref.: #32100]

GC-content

67.9 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Azospira restricta DSM 18626 NCBI: GCA_016858125.1
[Ref.: #69481]	spore-forming	no	97	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Azospira restricta DSM 18626 NCBI: GCA_016858125.1
[Ref.: #69480]	flagellated	yes	89.578	no
[Ref.: #69480]	gram-positive	no	99.321	yes
[Ref.: #69480]	anaerobic	no	92.403	yes
[Ref.: #69480]	aerobic	yes	59.701	yes
[Ref.: #69480]	halophile	no	96.087	yes
[Ref.: #69480]	spore-forming	no	95.135	yes
[Ref.: #69480]	thermophile	no	93.057	yes
[Ref.: #69480]	glucose-util	no	79.259	no
[Ref.: #69480]	motile	yes	90.29	no
[Ref.: #69480]	glucose-ferment	no	92.351	no

Availability in culture collections External links

[Ref.: #7626]

Culture collection no.

DSM 18626, CCUG 55598, NRRL B-41660, LMG 23819, Sua SUA2, Sua 2

[Ref.: #83068]

SI-ID 292492

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Genome Sequence of the Type Strain Azospira restricta SUA2 (DSM 18626).	Mikes MC, Moe WM	Microbiol Resour Announc	10.1128/MRA.00156-21	2021	*
Phylogeny	Description of Azospira restricta sp. nov., a nitrogen-fixing bacterium isolated from groundwater.	Bae HS, Rash BA, Rainey FA, Nobre MF, Tiago I, da Costa MS, Moe WM	Int J Syst Evol Microbiol	10.1099/ijs.0.64965-0	2007	*

References

#7626

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18626

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#32100

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28345 (see below)

#60664 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 55598

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#83068

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID292492.1 )

#28345

IJSEM 1521 2007 ( DOI 10.1099/ijs.0.64965-0 , PubMed 17625187 )

* These data were automatically processed and therefore are not curated

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