Levilactobacillus brevis (CIP 105136, ATCC 27305, CCRC 14040)

Name: Levilactobacillus brevis (CIP 105136, ATCC 27305, CCRC 14040)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 138175

Type strain

Strain Designation S8-40, P-2

Culture col. no. CIP 105136 ATCC 27305 CCRC 14040 CCUG 21958 JCM 1102 5 more

NCBI tax ID(s) 1580

Special Collections CCUG JCM CIP

History JCM <-- T. Mitsuoka S8-40 <-- ATCC 27305 <-- H. Nonomura P-2 <-- E. Peynaud. CIP <- 1997, JCM, "Lactobacillus brevis subsp. gravesensis" <- T. Mitsuoka: strain S8-40 <- ATCC <- H. Nonomura: strain P-2 <- E. Peynaud

Links

Levilactobacillus brevis S8-40 is a facultative anaerobe, Gram-positive, rod-shaped bacterium that was isolated from Wine.

Gram-positive rod-shaped facultative anaerobe genome sequence Bacteria

Name and taxonomic classification

SI-ID 3242

LMG 7934,ATCC 27305,JCM 1102,CCUG 21958,CCUG 21959,CIP 105136,CCRC 14040,BCRC 14040,KCTC 5065

@ref 20215

Last LPSN update 2026-05-29 08:39:12

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Levilactobacillus

Species Levilactobacillus brevis

Full scientific name Levilactobacillus brevis (Orla-Jensen 1919) Zheng et al. 2020

Synonyms (2)

"Betabacterium breve"
Lactobacillus brevis

BacDive ID	Other strains from **Levilactobacillus brevis** (23)	Type strain
6655	L. brevis 14, Bb14, Bb 14, DSM 20054, ATCC 14869, NCDO ... (type strain)
6650	L. brevis 5 N, DSM 1267, NCIB 8836
6651	L. brevis DSM 1268, NCIB 8561
6652	L. brevis DSM 1269, NCIB 8562
6653	L. brevis No. 2, DSM 2647
6654	L. brevis D 13, DSM 6235, CCM 7932, CIP 103474
6656	L. brevis 269Y, CCUG 21531, DSM 20556, ATCC 8287, CCM ...
139935	L. brevis FAL 25/53, DSM 13201
161016	L. brevis JCM 1065, LMG 12022
161017	L. brevis JCM 1066, NCTC 6107
162006	L. brevis JCM 17312
164748	L. brevis JCM 34252
165586	L. brevis JCM 7731
165597	L. brevis JCM 7753
165601	L. brevis JCM 7757
165602	L. brevis JCM 7758
165610	L. brevis JCM 7775
171318	L. brevis 370, CRBIP24.156
171319	L. brevis 63 AC, CRBIP24.61
171320	L. brevis 158-73, CRBIP24.71
171321	L. brevis CRBIP24.92, Br 23
171322	L. brevis CRBIP24.93, Br 391
175190	L. brevis HUN084, AG48, DSM 115837

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility	Confidence
39203	positive	rod-shaped
125438	positive			94.057

Culture and growth conditions

Culture medium

@ref	Name	Growth	Composition	Medium link
39203	MEDIUM 40- for Lactobacillus and Leuconostoc		Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
39203	CIP Medium 40			Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
39203	positive	growth	37
39203	positive	growth	15-37
39203	negative	growth	10
39203	negative	growth	41
67770	positive	growth	30

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
39203	facultative anaerobe
125439	obligate aerobe	96.191

Spore formation

@ref	Spore formation	Confidence
125439		97.954

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	-	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	+	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	+	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
39203	17632 ChEBI	nitrate	-	reduction
39203	17632 ChEBI	nitrate	+	respiration
39203	16301 ChEBI	nitrite	-	reduction
68371	0 ChEBI	Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	0 ChEBI	Potassium 5-ketogluconate	+	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
39203	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
39203	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
39203	alcohol dehydrogenase	+	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
39203	catalase	-	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
39203	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
39203	ornithine decarboxylase	-	4.1.1.17
39203	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	-		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	acetoin degradation	100	3 of 3
66794	palmitate biosynthesis	100	22 of 22
66794	acetate fermentation	100	4 of 4
66794	L-lactaldehyde degradation	100	3 of 3
66794	ribulose monophosphate pathway	100	2 of 2
66794	ppGpp biosynthesis	100	4 of 4
66794	formaldehyde oxidation	100	3 of 3
66794	coenzyme A metabolism	100	4 of 4
66794	teichoic acid biosynthesis	100	1 of 1
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	folate polyglutamylation	100	1 of 1
66794	vitamin B1 metabolism	92.31	12 of 13
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	valine metabolism	88.89	8 of 9
66794	serine metabolism	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	purine metabolism	82.98	78 of 94
66794	glutamate and glutamine metabolism	82.14	23 of 28
66794	pentose phosphate pathway	81.82	9 of 11
66794	methylglyoxal degradation	80	4 of 5
66794	threonine metabolism	80	8 of 10
66794	Entner Doudoroff pathway	80	8 of 10
66794	peptidoglycan biosynthesis	80	12 of 15
66794	photosynthesis	78.57	11 of 14
66794	glycolysis	76.47	13 of 17
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	C4 and CAM-carbon fixation	75	6 of 8
66794	ketogluconate metabolism	75	6 of 8
66794	sulfopterin metabolism	75	3 of 4
66794	isoleucine metabolism	75	6 of 8
66794	gluconeogenesis	75	6 of 8
66794	proline metabolism	72.73	8 of 11
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	heme metabolism	71.43	10 of 14
66794	phenylalanine metabolism	69.23	9 of 13
66794	pyrimidine metabolism	68.89	31 of 45
66794	degradation of sugar alcohols	68.75	11 of 16
66794	cyanate degradation	66.67	2 of 3
66794	cysteine metabolism	66.67	12 of 18
66794	NAD metabolism	66.67	12 of 18
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	d-xylose degradation	63.64	7 of 11
66794	alanine metabolism	62.07	18 of 29
66794	non-pathway related	60.53	23 of 38
66794	glycogen metabolism	60	3 of 5
66794	lipoate biosynthesis	60	3 of 5
66794	glycine betaine biosynthesis	60	3 of 5
66794	propionate fermentation	60	6 of 10
66794	oxidative phosphorylation	59.34	54 of 91
66794	arginine metabolism	58.33	14 of 24
66794	methionine metabolism	57.69	15 of 26
66794	tetrahydrofolate metabolism	57.14	8 of 14
66794	citric acid cycle	57.14	8 of 14
66794	propanol degradation	57.14	4 of 7
66794	degradation of sugar acids	56	14 of 25
66794	CO2 fixation in Crenarchaeota	55.56	5 of 9
66794	tryptophan metabolism	55.26	21 of 38
66794	vitamin B6 metabolism	54.55	6 of 11
66794	leucine metabolism	53.85	7 of 13
66794	histidine metabolism	51.72	15 of 29
66794	ethanol fermentation	50	1 of 2
66794	adipate degradation	50	1 of 2
66794	degradation of hexoses	50	9 of 18
66794	CMP-KDO biosynthesis	50	2 of 4
66794	butanoate fermentation	50	2 of 4
66794	glycine metabolism	50	5 of 10
66794	degradation of pentoses	50	14 of 28
66794	phenylmercury acetate degradation	50	1 of 2
66794	suberin monomers biosynthesis	50	1 of 2
66794	degradation of aromatic, nitrogen containing compounds	50	6 of 12
66794	urea cycle	46.15	6 of 13
66794	lysine metabolism	45.24	19 of 42
66794	lipid metabolism	45.16	14 of 31
66794	d-mannose degradation	44.44	4 of 9
66794	allantoin degradation	44.44	4 of 9
66794	glutathione metabolism	42.86	6 of 14
66794	tyrosine metabolism	42.86	6 of 14
66794	mevalonate metabolism	42.86	3 of 7
66794	reductive acetyl coenzyme A pathway	42.86	3 of 7
66794	ubiquinone biosynthesis	42.86	3 of 7
66794	phenylacetate degradation (aerobic)	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	gallate degradation	40	2 of 5
66794	sulfate reduction	38.46	5 of 13
66794	metabolism of disaccharids	36.36	4 of 11
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	octane oxidation	33.33	1 of 3
66794	lipid A biosynthesis	33.33	3 of 9
66794	sphingosine metabolism	33.33	2 of 6
66794	nitrate assimilation	33.33	3 of 9
66794	enterobactin biosynthesis	33.33	1 of 3
66794	4-hydroxymandelate degradation	33.33	3 of 9
66794	flavin biosynthesis	33.33	5 of 15
66794	androgen and estrogen metabolism	31.25	5 of 16
66794	phenylpropanoid biosynthesis	30.77	4 of 13
66794	starch degradation	30	3 of 10
66794	coenzyme M biosynthesis	30	3 of 10
66794	isoprenoid biosynthesis	26.92	7 of 26
66794	lactate fermentation	25	1 of 4
66794	biotin biosynthesis	25	1 of 4
66794	carnitine metabolism	25	2 of 8
66794	cyclohexanol degradation	25	1 of 4
66794	glycogen biosynthesis	25	1 of 4
66794	catecholamine biosynthesis	25	1 of 4
66794	dTDPLrhamnose biosynthesis	25	2 of 8
66794	vitamin B12 metabolism	23.53	8 of 34
66794	ascorbate metabolism	22.73	5 of 22
66794	arachidonic acid metabolism	22.22	4 of 18

Fatty acid profile

Metadata FA analysis

@ref

65607

Fatty acid	Percentage	ECL
C16:0	34	16
C19:0 CYCLO ω8c	15.2	18.9
C19:0 CYCLO ω9c	13	18.87
C18:2 ω6,9c/C18:0 ANTE	10	17.724
C18:0	7.8	18
C18:1 ω9c	7.7	17.769
C18:1 ω7c /12t/9t	6.8	17.824
C14:0	2.2	14
C16:1 ω7c	2.2	15.819
Unidentified	1	13.241

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
39203	-	-	-	-	-	+	-	+	-	-	+	+	+	+	-	+	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
39203	not determinedn.d.	-	-	-	-	+	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+

Isolation, sampling and environmental information

Isolation

@ref	Sample type
67770	Wine
39203	Food, Wine

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
39203	1	Risk group (French classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM15917v1 assembly for Lentilactobacillus hilgardii ATCC 27305	scaffold	GCA_000159175	525310.3	643886059	525310	42.82

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM15917v1 assembly for Lentilactobacillus hilgardii ATCC 27305
BV-BRC: 525310.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	gram_stain	BacteriaNetⓘ	negative	67.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	96.19	no
125439	motility	BacteriaNetⓘ	no	73.02	no
125439	spore_formation	BacteriaNetⓘ	no	97.95	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lactobacillus brevis subsp. gravesensis ATCC 27305
PATRIC: 525310.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	94.06	no
125438	anaerobic	anaerobicⓘ	no	83.89	yes
125438	spore-forming	spore-formingⓘ	no	77.06	no
125438	aerobic	aerobicⓘ	no	92.80	yes
125438	thermophilic	thermophileⓘ	no	96.49	no
125438	flagellated	motile2+ⓘ	no	89.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Exploiting Potential Probiotic Lactic Acid Bacteria Isolated from Chlorella vulgaris Photobioreactors as Promising Vitamin B12 Producers.	Ribeiro M, Maciel C, Cruz P, Darmancier H, Nogueira T, Costa M, Laranjeira J, Morais RMSC, Teixeira P.	Foods	10.3390/foods12173277	2023
	Flagellar Genes Are Associated with the Colonization Persistence Phenotype of the Drosophila melanogaster Microbiota.	Morgan SJ, Chaston JM.	Microbiol Spectr	10.1128/spectrum.04585-22	2023
	Role of plasmids in Lactobacillus brevis BSO 464 hop tolerance and beer spoilage.	Bergsveinson J, Baecker N, Pittet V, Ziola B.	Appl Environ Microbiol	10.1128/aem.02870-14	2015
Metabolism	A gut microbial factor modulates locomotor behaviour in Drosophila.	Schretter CE, Vielmetter J, Bartos I, Marka Z, Marka S, Argade S, Mazmanian SK.	Nature	10.1038/s41586-018-0634-9	2018
Genetics	Insights into the completely annotated genome of Lactobacillus buchneri CD034, a strain isolated from stable grass silage.	Heinl S, Wibberg D, Eikmeyer F, Szczepanowski R, Blom J, Linke B, Goesmann A, Grabherr R, Schwab H, Puhler A, Schluter A	J Biotechnol	10.1016/j.jbiotec.2012.03.007	2012

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#39203	Collection of Institut Pasteur ; Curators of the CIP; CIP 105136
#65607	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 21958
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Levilactobacillus brevis (CIP 105136, ATCC 27305, CCRC 14040)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Antibiotic resistance

Metabolite tests

Enzymes

Pathway annotation

Fatty acid profile

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000159175

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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