Name: Oceanobacillus kapialis SSK2-2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1378

Type strain:

Species: Oceanobacillus kapialis

Strain Designation: SSK2-2

Culture col. no.: DSM 23158, KCTC 13177, PCU 300, TISTR 1858

Strain history: <- S Tanasupawat, Chulalongkorn Univ., Thailand

NCBI tax ID(s): 481353 (species)

SI-ID 399584

DSM 23158

Section

Oceanobacillus kapialis SSK2-2 is an obligate aerobe, moderately halophilic, spore-forming bacterium that forms circular colonies and was isolated from fermented shrimp paste .

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
mesophilic
moderately halophilic
obligate aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Oceanobacillus*
	Species	**Oceanobacillus kapialis**
	Full Scientific Name (LPSN)	Oceanobacillus kapialis Namwong et al. 2009

Information on morphological and physiological properties Morphology

[Ref.: #23012]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive

[Ref.: #23012]	Cell length	0.8-3.0 µm

[Ref.: #23012]	Cell width	0.4-0.5 µm

[Ref.: #23012]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #23012]	Motility	yes
[Ref.: #67771]	Motility	yes

[Ref.: #23012]	Flagellum arrangement	peritrichous
[Ref.: #67771]	Flagellum arrangement	peritrichous

[Ref.: #23012]	Colony size	0.3-2.7 mm
[Ref.: #23012]	Colony color	white to cream
[Ref.: #23012]	Colony shape	circular

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16738]

Culture medium

HALOBACTERIA MEDIUM (DSMZ Medium 372)

[Ref.: #16738]

Culture medium growth

[Ref.: #16738]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16738]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #16738]	growth	30 °C
[Ref.: #23012]	growth	8.0-43.0 °C
[Ref.: #23012]	optimum	37.0 °C
[Ref.: #67771]	growth	30 °C

[Ref.: #16738]	Temperature range	mesophilic
[Ref.: #23012]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #23012]			optimum	8.0
[Ref.: #23012]			growth	6.0-9.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23012]	Oxygen tolerance	obligate aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #23012]	Spore description	oval,terminal,in swollen sporangia
[Ref.: #23012]	Type of spore	endospore
[Ref.: #23012]	Ability of spore formation	yes
[Ref.: #67771]	Type of spore	endospore
[Ref.: #67771]	Ability of spore formation	yes

[Ref.: #23012]

Halophily / tolerance level

moderately halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23012]		NaCl		growth	0.5-24.0	%(w/v)
[Ref.: #23012]		NaCl		optimum	6.0-14.0	%(w/v)

[Ref.: #67771]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23012]	amygdalin ChEBI	-	builds acid from
[Ref.: #23012]	casein	+	hydrolysis
[Ref.: #23012]	cellobiose ChEBI	-	builds acid from
[Ref.: #23012]	D-fructose ChEBI	+	builds acid from
[Ref.: #23012]	D-galactose ChEBI	-	builds acid from
[Ref.: #23012]	D-gluconate ChEBI	-	builds acid from
[Ref.: #23012]	D-glucose ChEBI	+	builds acid from
[Ref.: #23012]	D-mannitol ChEBI	+	builds acid from
[Ref.: #23012]	D-mannose ChEBI	+	builds acid from
[Ref.: #23012]	D-ribose ChEBI	-	builds acid from
[Ref.: #23012]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #23012]	D-xylose ChEBI	-	builds acid from
[Ref.: #23012]	esculin ChEBI	-	builds acid from
[Ref.: #23012]	esculin ChEBI	-	hydrolysis
[Ref.: #23012]	gelatin ChEBI	+	hydrolysis
[Ref.: #23012]	glycerol ChEBI	-	builds acid from
[Ref.: #23012]	hypoxanthine ChEBI	-	hydrolysis
[Ref.: #23012]	inulin ChEBI	-	builds acid from
[Ref.: #23012]	L-arabinose ChEBI	+	builds acid from
[Ref.: #23012]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #23012]	lactose ChEBI	-	builds acid from
[Ref.: #23012]	maltose ChEBI	+	builds acid from
[Ref.: #23012]	melezitose ChEBI	-	builds acid from
[Ref.: #23012]	melibiose ChEBI	-	builds acid from
[Ref.: #23012]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #23012]	myo-inositol ChEBI	-	builds acid from
[Ref.: #23012]	nitrate ChEBI	-	reduction
[Ref.: #23012]	raffinose ChEBI	-	builds acid from
[Ref.: #23012]	salicin ChEBI	-	builds acid from
[Ref.: #23012]	starch ChEBI	-	hydrolysis
[Ref.: #23012]	sucrose ChEBI	-	builds acid from
[Ref.: #23012]	trehalose ChEBI	-	builds acid from
[Ref.: #23012]	tween 80 ChEBI	-	hydrolysis
[Ref.: #23012]	tyrosine ChEBI	-	hydrolysis
[Ref.: #23012]	xanthine ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #23012]	catalase	+	1.11.1.6
[Ref.: #23012]	cytochrome oxidase	+	1.9.3.1
[Ref.: #23012]	DNase	-
[Ref.: #23012]	urease	-	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #23012]	Sample type/isolated from	fermented shrimp paste (ka-pi)
[Ref.: #23012]	Geographic location (country and/or sea, region)	market in Samutsakorn Province
[Ref.: #23012]	Country	Thailand
[Ref.: #23012]	Country ISO 3 Code	THA
[Ref.: #23012]	Continent	Asia
[Ref.: #23012]	Enrichment culture	agar plates of Lentibacillus medium (JCM medium no. 377),pH 7.2
[Ref.: #23012]	Enrichment culture composition	containing (l-1): 100 g NaCl,5 g Casamino acids, 5 g yeast extract, 1 g glutamic acid, 2 g KCl, 3 g trisodium citrate, 20 g MgSO4.7H2O, 36 mg FeCl2.4H2O, 0.36 mg MnCl2.4H2O and 20 g agar
[Ref.: #23012]	Enrichment culture duration	7 days
[Ref.: #23012]	Enrichment culture temperature	37.0 ̊C

[Ref.: #16738]	Sample type/isolated from	fermented shrimp paste (ka-pi)
[Ref.: #16738]	Geographic location (country and/or sea, region)	Samutsakorn Province
[Ref.: #16738]	Country	Thailand
[Ref.: #16738]	Country ISO 3 Code	THA
[Ref.: #16738]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From Shrimp paste (ka-pi)
[Ref.: #67771]	Country	Thailand
[Ref.: #67771]	Country ISO 3 Code	THA
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Seafood
#Engineered	#Food production	#Fermented

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16738]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16738]	Oceanobacillus kapialis gene for 16S ribosomal RNA, partial sequence, strain: SSK2-2	AB366005	1487	ENA	481353 tax ID

[Ref.: #16738]	GC-content	39.7 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67771]	GC-content	39.7 mol%

Availability in culture collections External links

[Ref.: #16738]

Culture collection no.

DSM 23158, KCTC 13177, PCU 300, TISTR 1858

[Ref.: #71029]

SI-ID 399584

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Oceanobacillus kapialis sp. nov., from fermented shrimp paste in Thailand.	Namwong S, Tanasupawat S, Lee KC, Lee JS	Int J Syst Evol Microbiol	10.1099/ijs.0.007161-0	2009	*

References

#16738

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23158

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23012

Sirilak Namwong,Somboon Tanasupawat,Keun Chul Lee,Jung-Sook Lee: Oceanobacillus kapialis sp. nov., from fermented shrimp paste in Thailand. IJSEM 59: 2254 - 2259 2009 ( DOI 10.1099/ijs.0.007161-0 , PubMed 19620352 )

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#71029

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID399584.1 )

* These data were automatically processed and therefore are not curated

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