Roseovarius tolerans (DSM 11463, EL-222, CIP 106399)

Name: Roseovarius tolerans (DSM 11463, EL-222, CIP 106399)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13767

Type strain

Strain Designation EL-222

Culture col. no. DSM 11463 EL-222 CIP 106399

NCBI tax ID(s) 74031

Special Collections DSMZ CIP

History <- M. Labrenz and P. Hirsch, EL-222 CIP <- 2000, P. Hirsch, Kiel Univ., Kiel, Germany: strain EL-222

Links

Roseovarius tolerans EL-222 is a Gram-negative, rod-shaped bacterium that was isolated from water.

Gram-negative rod-shaped Bacteria

Name and taxonomic classification

SI-ID 49116

CIP 106399,IFO 16696,NBRC 16696,DSM 11463

@ref 20215

Last LPSN update 2026-02-09 11:21:14

Domain Bacteria

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Rhodobacterales

Family Roseobacteraceae

Genus Roseovarius

Species Roseovarius tolerans

Full scientific name Roseovarius tolerans Labrenz et al. 1999

BacDive ID	Other strains from **Roseovarius tolerans** (2)	Type strain
13766	R. tolerans EL-172, DSM 11457, JCM 21346, CIP 106398, ... (type strain)
160494	R. tolerans EL-164, DSM 108135

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
123560	negative	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
4376	EKHO LAKE STRAINS MEDIUM (DSMZ Medium 621a)	Medium recipe at MediaDive	Name: EKHO LAKE STRAINS MEDIUM (DSMZ Medium 621a) Composition: NaCl 22.6553 g/l Agar 15.0 g/l MgCl2 x 6 H2O 4.80666 g/l Na2SO4 3.77991 g/l CaCl2 1.06343 g/l KCl 0.64076 g/l MgSO4 x 7 H2O 0.625264 g/l Glucose 0.25 g/l Peptone 0.25 g/l Yeast extract 0.25 g/l Nitrilotriacetic acid 0.210526 g/l NaHCO3 0.18528 g/l CaCl2 x 2 H2O 0.0703158 g/l H3BO3 0.02509 g/l SrCl2 0.02316 g/l KBr 0.00579 g/l NaF 0.002895 g/l FeSO4 x 7 H2O 0.00208422 g/l Na2MoO4 x 2 H2O 0.000266736 g/l Pyridoxine hydrochloride 0.0001 g/l D-Calcium pantothenate 5e-05 g/l Nicotinamide 5e-05 g/l Thiamine-HCl x 2 H2O 5e-05 g/l Riboflavine 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l Folic acid 2e-05 g/l Biotin 2e-05 g/l Vitamin B12 1e-06 g/l Metall salt sol. 44 Distilled water
35943	MEDIUM 168 - for Cytophaga latercula and Flammeovirga aprica		Agar (15.000 g);Yeast extract (0.500 g);Sodium acetate (0.200 g);Tryptone (2.000 g);Beef extract (0.500 g);Synthetic sea solution - M0216 (1000.000 ml)
123560	CIP Medium 168	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
4376	positive	growth	20
35943	positive	growth	22
123560	positive	growth	22-25
123560	negative	growth	5
123560	negative	growth	15
123560	negative	growth	30
123560	negative	growth	37
123560	negative	growth	41
123560	negative	growth	45

Physiology and metabolism

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
123560	4853 ChEBI	esculin	-	hydrolysis
123560	606565 ChEBI	hippurate	-	hydrolysis
123560	17632 ChEBI	nitrate	-	builds gas from
123560	17632 ChEBI	nitrate	-	reduction
123560	16301 ChEBI	nitrite	-	builds gas from

Metabolite production

@ref	Chebi-ID	Metabolite	Production
123560	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
123560	15688 ChEBI	acetoin	-
123560	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
123560	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
123560	amylase	-
68382	beta-galactosidase	-	3.2.1.23	from API zym
123560	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
123560	caseinase	-	3.4.21.50
123560	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
123560	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
123560	gamma-glutamyltransferase	-	2.3.2.2
123560	gelatinase	-
123560	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
123560	lipase	-
68382	lipase (C 14)	-		from API zym
123560	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
123560	ornithine decarboxylase	-	4.1.1.17
123560	oxidase	-
123560	phenylalanine ammonia-lyase	-	4.3.1.24
123560	protease	-
68382	trypsin	-	3.4.21.4	from API zym
123560	tryptophan deaminase	-
123560	tween esterase	-
123560	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	lactate fermentation	100	4 of 4
66794	gluconeogenesis	100	8 of 8
66794	C4 and CAM-carbon fixation	100	8 of 8
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	denitrification	100	2 of 2
66794	methylglyoxal degradation	100	5 of 5
66794	molybdenum cofactor biosynthesis	100	9 of 9
66794	reductive acetyl coenzyme A pathway	100	7 of 7
66794	coenzyme A metabolism	100	4 of 4
66794	adipate degradation	100	2 of 2
66794	formaldehyde oxidation	100	3 of 3
66794	glycine betaine biosynthesis	100	5 of 5
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	valine metabolism	100	9 of 9
66794	folate polyglutamylation	100	1 of 1
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	taurine degradation	100	1 of 1
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	suberin monomers biosynthesis	100	2 of 2
66794	palmitate biosynthesis	100	22 of 22
66794	octane oxidation	100	3 of 3
66794	ethylmalonyl-CoA pathway	100	5 of 5
66794	ppGpp biosynthesis	100	4 of 4
66794	butanoate fermentation	100	4 of 4
66794	proline metabolism	90.91	10 of 11
66794	threonine metabolism	90	9 of 10
66794	chorismate metabolism	88.89	8 of 9
66794	allantoin degradation	88.89	8 of 9
66794	serine metabolism	88.89	8 of 9
66794	vitamin B12 metabolism	85.29	29 of 34
66794	vitamin B1 metabolism	84.62	11 of 13
66794	phenylalanine metabolism	84.62	11 of 13
66794	leucine metabolism	84.62	11 of 13
66794	glycolate and glyoxylate degradation	83.33	5 of 6
66794	pentose phosphate pathway	81.82	9 of 11
66794	peptidoglycan biosynthesis	80	12 of 15
66794	Entner Doudoroff pathway	80	8 of 10
66794	phenylacetate degradation (aerobic)	80	4 of 5
66794	glutamate and glutamine metabolism	78.57	22 of 28
66794	photosynthesis	78.57	11 of 14
66794	citric acid cycle	78.57	11 of 14
66794	NAD metabolism	77.78	14 of 18
66794	d-mannose degradation	77.78	7 of 9
66794	purine metabolism	77.66	73 of 94
66794	sulfopterin metabolism	75	3 of 4
66794	CMP-KDO biosynthesis	75	3 of 4
66794	acetate fermentation	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	biotin biosynthesis	75	3 of 4
66794	isoleucine metabolism	75	6 of 8
66794	degradation of aromatic, nitrogen containing compounds	75	9 of 12
66794	flavin biosynthesis	73.33	11 of 15
66794	vitamin B6 metabolism	72.73	8 of 11
66794	histidine metabolism	72.41	21 of 29
66794	cysteine metabolism	72.22	13 of 18
66794	heme metabolism	71.43	10 of 14
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	tetrahydrofolate metabolism	71.43	10 of 14
66794	propanol degradation	71.43	5 of 7
66794	ubiquinone biosynthesis	71.43	5 of 7
66794	glutathione metabolism	71.43	10 of 14
66794	lipid metabolism	70.97	22 of 31
66794	arginine metabolism	70.83	17 of 24
66794	urea cycle	69.23	9 of 13
66794	methionine metabolism	69.23	18 of 26
66794	alanine metabolism	68.97	20 of 29
66794	cyanate degradation	66.67	2 of 3
66794	IAA biosynthesis	66.67	2 of 3
66794	methane metabolism	66.67	2 of 3
66794	CO2 fixation in Crenarchaeota	66.67	6 of 9
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	acetoin degradation	66.67	2 of 3
66794	aspartate and asparagine metabolism	66.67	6 of 9
66794	isoprenoid biosynthesis	65.38	17 of 26
66794	glycolysis	64.71	11 of 17
66794	tryptophan metabolism	63.16	24 of 38
66794	dTDPLrhamnose biosynthesis	62.5	5 of 8
66794	degradation of sugar alcohols	62.5	10 of 16
66794	lysine metabolism	61.9	26 of 42
66794	oxidative phosphorylation	61.54	56 of 91
66794	chlorophyll metabolism	61.11	11 of 18
66794	non-pathway related	60.53	23 of 38
66794	factor 420 biosynthesis	60	3 of 5
66794	propionate fermentation	60	6 of 10
66794	pyrimidine metabolism	60	27 of 45
66794	phenol degradation	60	12 of 20
66794	lipid A biosynthesis	55.56	5 of 9
66794	sulfate reduction	53.85	7 of 13
66794	3-phenylpropionate degradation	53.33	8 of 15
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	carnitine metabolism	50	4 of 8
66794	coenzyme M biosynthesis	50	5 of 10
66794	kanosamine biosynthesis II	50	1 of 2
66794	toluene degradation	50	2 of 4
66794	tyrosine metabolism	50	7 of 14
66794	ketogluconate metabolism	50	4 of 8
66794	ethanol fermentation	50	1 of 2
66794	phenylmercury acetate degradation	50	1 of 2
66794	glycogen biosynthesis	50	2 of 4
66794	selenocysteine biosynthesis	50	3 of 6
66794	glycine metabolism	50	5 of 10
66794	degradation of pentoses	46.43	13 of 28
66794	phosphatidylethanolamine bioynthesis	46.15	6 of 13
66794	4-hydroxymandelate degradation	44.44	4 of 9
66794	degradation of sugar acids	40	10 of 25
66794	glycogen metabolism	40	2 of 5
66794	vitamin K metabolism	40	2 of 5
66794	lipoate biosynthesis	40	2 of 5
66794	hydrogen production	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	degradation of hexoses	38.89	7 of 18
66794	androgen and estrogen metabolism	37.5	6 of 16
66794	polyamine pathway	34.78	8 of 23
66794	nitrate assimilation	33.33	3 of 9
66794	enterobactin biosynthesis	33.33	1 of 3
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	arachidonic acid metabolism	33.33	6 of 18
66794	pantothenate biosynthesis	33.33	2 of 6
66794	sphingosine metabolism	33.33	2 of 6
66794	sulfoquinovose degradation	33.33	1 of 3
66794	carotenoid biosynthesis	31.82	7 of 22
66794	ascorbate metabolism	31.82	7 of 22
66794	4-hydroxyphenylacetate degradation	30	3 of 10
66794	myo-inositol biosynthesis	30	3 of 10
66794	bile acid biosynthesis, neutral pathway	29.41	5 of 17
66794	benzoyl-CoA degradation	28.57	2 of 7
66794	aclacinomycin biosynthesis	28.57	2 of 7
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	metabolism of disaccharids	27.27	3 of 11
66794	d-xylose degradation	27.27	3 of 11
66794	cyclohexanol degradation	25	1 of 4
66794	alginate biosynthesis	25	1 of 4

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123560	-	+	+	+	-	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Environmental	#Aquatic

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
4376	water	Ekho Lake			Australia and Oceania
123560	Environment, Water samples	Ekho lake	Antarctica	ATA	Antarctica

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
4376	1	Risk group (German classification) According to TRBA 466
123560	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome-based predictions

Literature

References

#4376	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 11463
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#35943	; Curators of the CIP;
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#68382	Automatically annotated from API zym .
#123560	Collection of Institut Pasteur ; Curators of the CIP; CIP 106399
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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