Name: Roseobacter litoralis DSM 6996
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13764

Type strain:

Species: Roseobacter litoralis

Culture col. no.: DSM 6996, ATCC 49566, OCh 149, JCM 21268, CIP 104265, IAM 14593, IFO 15278, NBRC 15278, ATCC 46566

Strain history: CIP <- 1994, M. Gauthier, INSERM, Nice, France <- ATCC <- T. Shiba: strain OCh149

NCBI tax ID(s): 391595 (strain), 42443 (species)

SI-ID 14629

LMG 19750, ATCC 49566, DSM 6996, CIP 104265, NCIMB 13392, IFO 15278, CECT 5395, NBRC 15278, IAM 14593, JCM 21268

Section

Roseobacter litoralis DSM 6996 is a psychrophilic, Gram-negative, motile bacterium that was isolated from seaweed.

Gram-negative
motile
rod-shaped
psychrophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Rhodobacterales*
	Family	*Roseobacteraceae*
	Genus	*Roseobacter*
	Species	**Roseobacter litoralis**
	Full Scientific Name (LPSN)	Roseobacter litoralis Shiba 1991

Information on morphological and physiological properties Morphology

[Ref.: #120634]	Gram stain	negative

[Ref.: #120634]	Cell shape	rod-shaped

[Ref.: #120634]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #2939]

Culture medium

MEDIUM FOR ERYTHROBACTER LONGUS (DSMZ Medium 695)

[Ref.: #2939]

Culture medium growth

[Ref.: #2939]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2939]

Culture medium composition

expand / minimize

[Ref.: #2939]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #2939]

Culture medium growth

[Ref.: #2939]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2939]

Culture medium composition

expand / minimize

[Ref.: #41077]

Culture medium

Marine agar (MA)

[Ref.: #41077]

Culture medium growth

[Ref.: #41077]

Culture medium composition

expand / minimize

[Ref.: #120634]

Culture medium

CIP Medium 13

[Ref.: #120634]

Culture medium growth

[Ref.: #120634]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #2939]		growth	20 °C
[Ref.: #41077]		growth	22 °C
[Ref.: #67770]		growth	25 °C
[Ref.: #120634]		growth	5-30 °C
[Ref.: #120634]	no	growth	37 °C

[Ref.: #2939]	Temperature range	psychrophilic
[Ref.: #41077]	Temperature range	psychrophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #120634]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67770]

Observation

quinones: Q-10

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #120634]	nitrate ChEBI	-	reduction
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #120634]	nitrite ChEBI	-	reduction
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #120634]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes	no

	Metabolite	Production
[Ref.: #67770]	bacteriochlorophyll a ChEBI	yes
[Ref.: #120634]	indole ChEBI	no
[Ref.: #68369]	indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #120634]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #120634]	amylase	-
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #120634]	beta-galactosidase	+	3.2.1.23
[Ref.: #68369]	beta-glucosidase	+	3.2.1.21	* from API 20NE
[Ref.: #120634]	catalase	+	1.11.1.6
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #120634]	gelatinase	-
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #120634]	lysine decarboxylase	-	4.1.1.18
[Ref.: #120634]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #120634]	oxidase	+
[Ref.: #120634]	tween esterase	-
[Ref.: #120634]	urease	+	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #2939]	24467	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #2939]	24465	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #2939]	Sample type/isolated from	seaweed
[Ref.: #2939]	Country	Japan
[Ref.: #2939]	Country ISO 3 Code	JPN
[Ref.: #2939]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Seaweed
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia

[Ref.: #120634]	Sample type/isolated from	Seaweed
[Ref.: #120634]	Country	Japan
[Ref.: #120634]	Country ISO 3 Code	JPN
[Ref.: #120634]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Aquatic plant
#Environmental	#Aquatic	-
#Host	#Algae	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence X78312 (>99% sequence identity) for Sulfitobacter from Microbeatlas

Aquatic Samples	1065
Soil Samples	18
Animal Samples	24
Plant Samples	17
Total Samples	1124

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2939]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120634]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Roseobacter litoralis gene for 16S ribosomal RNA, partial sequence, strain: JCM 21268	LC069037	1386	ENA	42443 tax ID
[Ref.: #2939]	Roseobacter litoralis strain Och 149 16S ribosomal RNA, partial sequence	NR_027593	1369	GenBank	42443 tax ID
[Ref.: #20218]	R.litoralis (ATCC 49566) gene for 16S ribosomal RNA	X78312	1369	ENA	391595 tax ID	*
[Ref.: #20218]	Roseobacter litoralis 16S rRNA gene, 23S rRNA gene and internal transcribed spacer (ITS), strain DSM 6996	AJ012707	984	ENA	391595 tax ID	*
[Ref.: #20218]	Roseobacter litoralis strain NBRC15278 16S ribosomal RNA gene, partial sequence	DQ915624	1307	ENA	42443 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Roseobacter litoralis Och 149	GCA_000154785	complete	GenBank	391595 tax ID
[Ref.: #66792]	Roseobacter litoralis Och 149	391595.25	plasmid	PATRIC	391595 tax ID	*
[Ref.: #66792]	Roseobacter litoralis Och 149	391595.26	plasmid	PATRIC	391595 tax ID	*
[Ref.: #66792]	Roseobacter litoralis Och 149	391595.27	plasmid	PATRIC	391595 tax ID	*

[Ref.: #67770]

GC-content

57-57.4 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #2939]

Culture collection no.

DSM 6996, ATCC 49566, OCh 149, JCM 21268, CIP 104265, IAM 14593, IFO 15278, NBRC 15278, ATCC 46566

[Ref.: #82943]

SI-ID 14629

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Roseobacter cerasinus sp. nov., isolated from a fish farm.	Muramatsu S, Kanamuro M, Sato-Takabe Y, Hirose S, Muramatsu Y, Takaichi S, Hanada S	Int J Syst Evol Microbiol	10.1099/ijsem.0.004360	2020	*
Phylogeny	Roseobacter ponti sp. nov., isolated from seawater.	Jung YT, Park S, Lee JS, Yoon JH	Int J Syst Evol Microbiol	10.1099/ijsem.0.001922	2017	*
Metabolism	Proteomic responses of Roseobacter litoralis OCh149 to starvation and light regimen.	Zong R, Jiao N	Microbes Environ	10.1264/jsme2.me12029	2012	*
Metabolism	Formation of polyhydroxyalkanoate in aerobic anoxygenic phototrophic bacteria and its relationship to carbon source and light availability.	Xiao N, Jiao N	Appl Environ Microbiol	10.1128/AEM.05955-11	2011	*
Genetics	Comparative genome analysis and genome-guided physiological analysis of Roseobacter litoralis.	Kalhoefer D, Thole S, Voget S, Lehmann R, Liesegang H, Wollher A, Daniel R, Simon M, Brinkhoff T	BMC Genomics	10.1186/1471-2164-12-324	2011	*
Metabolism	Genome organization and localization of the pufLM genes of the photosynthesis reaction center in phylogenetically diverse marine Alphaproteobacteria.	Pradella S, Allgaier M, Hoch C, Pauker O, Stackebrandt E, Wagner-Dobler I	Appl Environ Microbiol	10.1128/AEM.70.6.3360-3369.2004	2004	*

References

#2939

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 6996

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#41077 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#82943

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID14629.1 )

#120634 Collection of Institut Pasteur ; Curators of the CIP; CIP 104265
* These data were automatically processed and therefore are not curated

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