Levilactobacillus parabrevis (CIP 109963, LMG 11984, ATCC 53295)

Name: Levilactobacillus parabrevis (CIP 109963, LMG 11984, ATCC 53295)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 137411

Type strain

Culture col. no. CIP 109963 LMG 11984 ATCC 53295 BCRC 81080 JCM 30911

NCBI tax ID(s) 357278

Special Collections JCM CIP

History LMG 11984 <-- ATCC 53295 (Lactobacillus brevis) <-- M. A. Spiller; RODS-DW, SLB-MAS (Sporolactobacillus sp.). CIP <- 2009, BCCM/LMG <- 1922, ATCC as Lactobacillus brevis <- M. Spiller as Sporolactobacillus sp. RODS-DW, Spiller SLB-MAS

Links

Levilactobacillus parabrevis CIP 109963 is a facultative anaerobe, mesophilic, Gram-positive prokaryote that was isolated from Wheat.

Gram-positive rod-shaped facultative anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 3232

LMG 11984,ATCC 53295,CIP 109963,JCM 30911

@ref 20215

Last LPSN update 2026-02-09 11:32:53

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Levilactobacillus

Species Levilactobacillus parabrevis

Full scientific name Levilactobacillus parabrevis (Vancanneyt et al. 2006) Zheng et al. 2020

Synonyms (1)

Lactobacillus parabrevis

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
125438			90.5
125438	positive		93.527
37888	positive	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Growth	Composition	Medium link
37888	MEDIUM 40- for Lactobacillus and Leuconostoc		Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
37888	CIP Medium 40			Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
37888	positive	growth	37	mesophilic
37888	positive	growth	15-37
37888	negative	growth	45
67770	positive	growth	28	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
37888	facultative anaerobe
125439	obligate aerobe	90.6

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
37888	17632 ChEBI	nitrate	-	reduction
37888	17632 ChEBI	nitrate	+	respiration
37888	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
37888	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
37888	15688 ChEBI	acetoin	+

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
37888	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	+	3.2.1.31	from API zym
37888	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
37888	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
37888	ornithine decarboxylase	+	4.1.1.17
37888	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
37888	urease	-	3.5.1.5
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
37888	-	-	+	+	-	+	+	-	-	-	+	+	-	-	+	+	+	+	-	-

Isolation, sampling and environmental information

Isolation

@ref	Sample type
67770	Wheat
37888	Food, Wheat

Taxonmaps

69479

Global distribution of 16S sequence AM158249 (>99% sequence identity) for Levilactobacillus from Microbeatlas

Aquatic Samples	28
Soil Samples	53
Animal Samples	264
Plant Samples	61
Total Samples	406

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
37888	1	Risk group (French classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM38343v1 assembly for Levilactobacillus parabrevis ATCC 53295 RODS-DW	scaffold	GCA_000383435	1267003.3	2519103178	1267003	60.4
67770	ASM143405v1 assembly for Levilactobacillus parabrevis ATCC 53295	contig	GCA_001434055	1267003.4	2657245447	1267003	52.92
66792	ASM143751v1 assembly for Levilactobacillus parabrevis LMG 11984	scaffold	GCA_001437515	357278.3	2667527803	357278	38.98

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
67770	Lactobacillus parabrevis 16S rRNA gene, type strain LMG 11984T	AM158249	1518		357278

GC content

@ref	GC-content (mol%)	Method
67770	49	high performance liquid chromatography (HPLC)
67770	49.1	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Levilactobacillus parabrevis ATCC 53295 RODS-DW
NCBI: GCA_000383435

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	90.60	no
125439	gram_stain	BacteriaNetⓘ	positive	85.20	no
125439	motility	BacteriaNetⓘ	yes	53.40	no
125439	spore_formation	BacteriaNetⓘ	yes	53.30	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Levilactobacillus parabrevis ATCC 53295 RODS-DW
NCBI: GCA_000383435.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	93.53	no
125438	anaerobic	anaerobicⓘ	no	84.65	yes
125438	aerobic	aerobicⓘ	no	96.88	no
125438	spore-forming	spore-formingⓘ	no	87.92	no
125438	thermophilic	thermophileⓘ	no	96.00	yes
125438	flagellated	motile2+ⓘ	no	90.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Companilactobacillus crustorum LMG 23699 and Wickerhamomyces anomalus IMDO 010110 form a candidate, stable, mixed-strain starter culture for sourdough production.	Pradal I, Gonzalez-Alonso V, Wardhana YR, De Vuyst L.	Int J Food Microbiol	10.1016/j.ijfoodmicro.2025.111278	2025
	Lacto-fermented garlic handcrafted in the Lower Silesia Region (Poland): Microbial diversity, morpho-textural traits, and volatile compounds	Cardinali F, Botta C, Harasym J, Ferrocino I, Reale A, Boscaino F, Di Renzo T, Milanovic V, Garofalo C, Rampanti G, Aquilanti L, Osimani A.	Food Research International.		2024
Biotechnology	Does strain-level persistence of lactobacilli in long-term back-slopped sourdoughs inform on domestication of food-fermenting lactic acid bacteria?	Pham VD, Xu ZS, Simpson DJ, Zhang JS, Ganzle MG.	Appl Environ Microbiol	10.1128/aem.01892-24	2024
	Slow food for lactobacilli: characterization of 1,2-propanediol metabolism in Levilactobacillus parabrevis FUA3697 in sourdough.	Pham VD, Ganzle MG.	Appl Environ Microbiol	10.1128/aem.01207-25	2025
Biotechnology	Lacto-fermented garlic handcrafted in the Lower Silesia Region (Poland): Microbial diversity, morpho-textural traits, and volatile compounds.	Cardinali F, Botta C, Harasym J, Ferrocino I, Reale A, Boscaino F, Di Renzo T, Milanovic V, Garofalo C, Rampanti G, Aquilanti L, Osimani A.	Food Res Int	10.1016/j.foodres.2024.114484	2024
	A multi-functional survey of the properties of Lacticaseibacillus paracasei subsp. tolerans NOC-122, Levilactobacillus parabrevis NOC-111 and Latilactobacillus curvatus NOC-110.	Ozdemir N.	Microbiology (Reading)	10.1099/mic.0.001239	2022
	Indigenous Lactic Acid Bacteria Isolated from Raw Graviera Cheese and Evaluation of Their Most Important Technological Properties.	Psomas E, Sakaridis I, Boukouvala E, Karatzia MA, Ekateriniadou LV, Samouris G.	Foods	10.3390/foods12020370	2023
	Characterization of microbiota of naturally fermented sauerkraut by high-throughput sequencing.	Zhang S, Zhang Y, Wu L, Zhang L, Wang S.	Food Sci Biotechnol	10.1007/s10068-022-01221-w	2023
	Evaluation of the variations in chemical and microbiological properties of the sourdoughs produced with selected lactic acid bacteria strains during fermentation.	Boyaci Gunduz CP, Agirman B, Gaglio R, Franciosi E, Francesca N, Settanni L, Erten H.	Food Chem X	10.1016/j.fochx.2022.100357	2022
	Microbial Communities and Physiochemical Properties of Four Distinctive Traditionally Fermented Vegetables from North China and Their Influence on Quality and Safety.	Liang T, Xie X, Wu L, Li L, Li H, Xi Y, Feng Y, Xue L, Chen M, Chen X, Zhang J, Ding Y, Wu Q.	Foods	10.3390/foods11010021	2021
	Microbial communities of a variety of 75 homemade fermented vegetables.	Thierry A, Madec MN, Chuat V, Bage AS, Picard O, Grondin C, Rue O, Mariadassou M, Marche L, Valence F.	Front Microbiol	10.3389/fmicb.2023.1323424	2023
	Metabolic and Gut Microbiota Responses to Sourdough Pasta Consumption in Overweight and Obese Adults.	Shah S, Brown PDS, Mayengbam S, Ganzle MG, Wang W, Mu C, Lettrari S, Bertagnolli C, Shearer J.	Front Nutr	10.3389/fnut.2020.615003	2020
	Physicochemical and sensory characterization of functional synbiotic Labneh fortified with the bacteriocin-producing Lactiplantibacillus plantarum strain GA7 and nano-encapsulated Tirmania pinoyi extract.	Daba GM, Elkhateeb WA, Saleh SAA, Soliman TN, El-Dein AN.	Microb Cell Fact	10.1186/s12934-024-02631-7	2025
	Screening of GABA-Producing Lactic Acid Bacteria from Thai Fermented Foods and Probiotic Potential of Levilactobacillus brevis F064A for GABA-Fermented Mulberry Juice Production.	Kanklai J, Somwong TC, Rungsirivanich P, Thongwai N.	Microorganisms	10.3390/microorganisms9010033	2020
	Surface layer proteins in species of the family Lactobacillaceae.	Palomino MM, Allievi MC, Gordillo TB, Bockor SS, Fina Martin J, Ruzal SM.	Microb Biotechnol	10.1111/1751-7915.14230	2023
Enzymology	Purification, properties and structural aspects of a thermoacidophilic alpha-amylase from Alicyclobacillus acidocaldarius atcc 27009. Insight into acidostability of proteins.	Schwermann B, Pfau K, Liliensiek B, Schleyer M, Fischer T, Bakker EP.	Eur J Biochem	10.1111/j.1432-1033.1994.00981.x	1994
Biotechnology	Lacticaseibacillus salsurae sp. nov. and Levilactobacillus muriae sp. nov., isolated from traditional Chinese pickle.	Song ST, Wang TY, Zhu XY, Gu CT.	Int J Syst Evol Microbiol	10.1099/ijsem.0.006816	2025
	Pyomelanin-Producing Brevundimonas vitisensis sp. nov., Isolated From Grape (Vitis vinifera L.).	Jiang L, Jeon D, Kim J, Lee CW, Peng Y, Seo J, Lee JH, Paik JH, Kim CY, Lee J.	Front Microbiol	10.3389/fmicb.2021.733612	2021
Phylogeny	Meganema perideroedes gen. nov., sp. nov., a filamentous alphaproteobacterium from activated sludge.	Thomsen TR, Blackall LL, de Muro MA, Nielsen JL, Nielsen PH.	Int J Syst Evol Microbiol	10.1099/ijs.0.02916-0	2006
Phylogeny	Characterization of two novel pentose-fermenting and GABA-producing species: Levilactobacillus tujiorum sp. nov. and Secundilactobacillus angelensis sp. nov. Isolated from a solid-state fermented zha-chili.	Zhang Z, Wang Y, Dong Y, Xiang F, Zhang Y, Zhang H, Sun Y, Guo Z	Syst Appl Microbiol	10.1016/j.syapm.2022.126344	2022

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#37888	Collection of Institut Pasteur ; Curators of the CIP; CIP 109963
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Levilactobacillus parabrevis (CIP 109963, LMG 11984, ATCC 53295)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

Isolation, sampling and environmental information

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000383435

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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