Name: Oceanobacillus iheyensis HTE831
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1372

Type strain:

Species: Oceanobacillus iheyensis

Strain Designation: HTE831

Culture col. no.: DSM 14371, JCM 11309, CGMCC 1.8876, CIP 107618, KCTC 3954

Strain history: CIP <- 2002, JCM <- 2001, H. Takami: strain HTE831

NCBI tax ID(s): 182710 (species)

SI-ID 100830

DSM 14371, JCM 11309, CIP 107618, KCTC 3954

Section

Oceanobacillus iheyensis HTE831 is a mesophilic, Gram-positive, motile bacterium that was isolated from deep sea sediment, 1050 m depth.

Gram-positive
motile
rod-shaped
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Oceanobacillus*
	Species	**Oceanobacillus iheyensis**
	Full Scientific Name (LPSN)	Oceanobacillus iheyensis Lu et al. 2002

Information on morphological and physiological properties Morphology

[Ref.: #120046]	Gram stain	positive

[Ref.: #120046]	Cell shape	rod-shaped

[Ref.: #120046]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5326]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #5326]

Culture medium growth

[Ref.: #5326]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5326]

Culture medium composition

expand / minimize

[Ref.: #37958]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #37958]

Culture medium growth

[Ref.: #37958]

Culture medium composition

expand / minimize

[Ref.: #120046]

Culture medium

CIP Medium 648

[Ref.: #120046]

Culture medium growth

[Ref.: #120046]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #120046]

Culture medium

CIP Medium 72

[Ref.: #120046]

Culture medium growth

[Ref.: #120046]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #5326]		growth	28 °C
[Ref.: #37958]		growth	30 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #120046]		growth	22-37 °C
[Ref.: #120046]	no	growth	10 °C
[Ref.: #120046]	no	growth	45 °C

[Ref.: #5326]	Temperature range	mesophilic
[Ref.: #37958]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #120046]	Temperature range	psychrophilic
[Ref.: #120046]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #120046]		no	growth	6

Information on physiology and metabolism Physiology and metabolism

	Salt	Tested relation		Salt conc.
[Ref.: #120046]	NaCl		growth	4-10	%
[Ref.: #120046]	NaCl	no	growth	0	%
[Ref.: #120046]	NaCl	no	growth	2	%

[Ref.: #67770]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #120046]	citrate ChEBI	-	carbon source
[Ref.: #120046]	esculin ChEBI	-	hydrolysis
[Ref.: #120046]	hippurate ChEBI	+	hydrolysis
[Ref.: #120046]	nitrate ChEBI	-	reduction
[Ref.: #120046]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #120046]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #120046]		acetoin ChEBI	-
[Ref.: #120046]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #120046]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #120046]	amylase	-
[Ref.: #120046]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #120046]	caseinase	-	3.4.21.50
[Ref.: #120046]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #120046]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #120046]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #120046]	gelatinase	-
[Ref.: #120046]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #120046]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #120046]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #120046]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #120046]	oxidase	-
[Ref.: #120046]	protease	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #120046]	tween esterase	-
[Ref.: #120046]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #120046]	2347	not determinedn.d.	+	-	-	-	-	-	-	-	-	+	+	-	-	-	-	+	+	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120046]	10513	-	-	+	+	-	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5326]	Sample type/isolated from	deep sea sediment, 1050 m depth
[Ref.: #5326]	Geographic location (country and/or sea, region)	Nanse,Okinawa, Iheya Ridge
[Ref.: #5326]	Country	Japan
[Ref.: #5326]	Country ISO 3 Code	JPN
[Ref.: #5326]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Deep-sea mud sample collected at a depth of 1,050 m on the Iheya Ridge of the Nansei Islands

[Ref.: #120046]	Sample type/isolated from	Environment, Deep sea mud
[Ref.: #120046]	Geographic location (country and/or sea, region)	Samples collected from the Iheya Ridge of the Nansei Islands (1050 m deep) by means of the manned submersible Shinkai 2000 ((Takagawa et al. 1989)
[Ref.: #120046]	Country	Japan
[Ref.: #120046]	Country ISO 3 Code	JPN
[Ref.: #120046]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB010863 (>99% sequence identity) for Oceanobacillus from Microbeatlas

Aquatic Samples	673
Soil Samples	2801
Animal Samples	830
Plant Samples	952
Total Samples	5256

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5326]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120046]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5326]	Oceanobacillus iheyensis HTE831 gene for 16S ribosomal RNA, partial sequence	AB010863	1525	ENA	221109 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Oceanobacillus iheyensis HTE831	GCA_000011245	complete	GenBank	221109 tax ID	*
[Ref.: #66792]	Oceanobacillus iheyensis HTE831	221109.4	complete	PATRIC	221109 tax ID	*
[Ref.: #66792]	Oceanobacillus iheyensis HTE831	637000201	complete	JGI IMG/M	221109 tax ID	*

[Ref.: #5326]	GC-content	35.8 mol%
[Ref.: #67770]	GC-content	35.8 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Oceanobacillus iheyensis HTE831 HTE831 NCBI: GCA_000011245.1
[Ref.: #69480]	motile	yes	86.252	no
[Ref.: #69480]	flagellated	yes	84.798	no
[Ref.: #69480]	gram-positive	yes	94.077	no
[Ref.: #69480]	anaerobic	no	99.449	no
[Ref.: #69480]	halophile	yes	92.543	no
[Ref.: #69480]	spore-forming	yes	95.287	no
[Ref.: #69480]	thermophile	no	99.503	yes
[Ref.: #69480]	glucose-util	yes	88.523	no
[Ref.: #69480]	aerobic	yes	95.297	no
[Ref.: #69480]	glucose-ferment	no	86.87	no

Availability in culture collections External links

[Ref.: #5326]

Culture collection no.

DSM 14371, JCM 11309, CGMCC 1.8876, CIP 107618, KCTC 3954

[Ref.: #71023]

SI-ID 100830

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Oceanobacillus pacificus sp. nov., isolated from a deep-sea sediment.	Yu C, Yu S, Zhang Z, Li Z, Zhang XH	Int J Syst Evol Microbiol	10.1099/ijs.0.056481-0	2014	*
Metabolism	Biochemical and mutational analysis of a novel nicotinamidase from Oceanobacillus iheyensis HTE831.	Sanchez-Carron G, Garcia-Garcia MI, Zapata-Perez R, Takami H, Garcia-Carmona F, Sanchez-Ferrer A	PLoS One	10.1371/journal.pone.0056727	2013	*
Phylogeny	Oceanobacillus neutriphilus sp. nov., isolated from activated sludge in a bioreactor.	Yang JY, Huo YY, Xu XW, Meng FX, Wu M, Wang CS	Int J Syst Evol Microbiol	10.1099/ijs.0.016295-0	2009	*
Enzymology	Characterization of endogenous pyridoxal 5'-phosphate-dependent alanine racemase from Bacillus pseudofirmus OF4.	Ju J, Xu S, Wen J, Li G, Ohnishi K, Xue Y, Ma Y	J Biosci Bioeng	10.1016/j.jbiosc.2008.11.005	2009	*
Genetics	CGAS: a comparative genome annotation system.	Choi K, Yang Y, Kim S	Methods Mol Biol	1-59745-514-8:133	2007	*
Phylogeny	Oceanobacillus chironomi sp. nov., a halotolerant and facultatively alkaliphilic species isolated from a chironomid egg mass.	Raats D, Halpern M	Int J Syst Evol Microbiol	10.1099/ijs.0.64502-0	2007	*
Metabolism	Multidimensional analysis of the insoluble sub-proteome of Oceanobacillus iheyensis HTE831, an alkaliphilic and halotolerant deep-sea bacterium isolated from the Iheya ridge.	Graham RL, Pollock CE, O'Loughlin SN, Ternan NG, Weatherly DB, Tarleton RL, McMullan G	Proteomics	10.1002/pmic.200600665	2007	*
Phylogeny	Oceanobacillus oncorhynchi subsp. incaldanensis subsp. nov., an alkalitolerant halophile isolated from an algal mat collected from a sulfurous spring in Campania (Italy), and emended description of Oceanobacillus oncorhynchi.	Romano I, Lama L, Nicolaus B, Poli A, Gambacorta A, Giordano A	Int J Syst Evol Microbiol	10.1099/ijs.0.63575-0	2006	*
Metabolism	D-Amino acid dipeptide production utilizing D-alanine-D-alanine ligases with novel substrate specificity.	Sato M, Kirimura K, Kino K	J Biosci Bioeng	10.1263/jbb.99.623	2005	*
Genetics	Identification and distribution of new insertion sequences in the genome of the extremely halotolerant and alkaliphilic Oceanobacillus iheyensis HTE831.	Takaki Y, Matsuki A, Chee GJ, Takami H	DNA Res	10.1093/dnares/11.4.233	2004	*
Genetics	Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments.	Takami H, Takaki Y, Uchiyama I	Nucleic Acids Res	10.1093/nar/gkf526	2002	*
Phylogeny	Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge.	Lu J, Nogi Y, Takami H	FEMS Microbiol Lett	10.1111/j.1574-6968.2001.tb10963.x	2001	*

References

#5326

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14371

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#37958 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#71023

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID100830.1 )

#120046 Collection of Institut Pasteur ; Curators of the CIP; CIP 107618
* These data were automatically processed and therefore are not curated

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Oceanobacillus iheyensis HTE831 HTE831

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