Dinoroseobacter shibae DFL 12 | Type strain | DSM 16493

Strain identifier

BacDive ID: 13662

Type strain:

Species: Dinoroseobacter shibae

Strain Designation: DFL 12

Culture col. no.: DSM 16493

Section

Name and taxonomic classification
Morphology and physiology
Culture and growth conditions
Isolation, sampling and environmental information
Application and interaction
Molecular biology
Strain availability

External links

Information on the name and the taxonomic classification.Name and taxonomic classification

[Ref.: #6375]	Domain	Bacteria
[Ref.: #6375]	Phylum	Proteobacteria
[Ref.: #6375]	Class	Alphaproteobacteria
[Ref.: #6375]	Order	Rhodobacterales
[Ref.: #6375]	Family	Rhodobacteraceae
[Ref.: #6375]	Genus	Dinoroseobacter
[Ref.: #6375]	Species	Dinoroseobacter shibae
[Ref.: #6375]	Full Scientific Name	Dinoroseobacter shibae Biebl et al. 2005
[Ref.: #6375]	Strain Designation	DFL 12
[Ref.: #6375]	Type strain

		Prokaryotic Nomenclature Up-to-date (PNU)	Taxonomical status	Literature reference
	Last PNU update	14-08-2019 (DD-MM-YYYY)
[Ref.: #20215]	Domain	Bacteria
[Ref.: #20215]	Phylum	Proteobacteria
[Ref.: #20215]	Class	Alphaproteobacteria		Int. J. Syst. Evol. Microbiol. 56:1
[Ref.: #20215]	Family	Rhodobacteraceae		Int. J. Syst. Evol. Microbiol. 56:1
[Ref.: #20215]	Genus	Dinoroseobacter	gen. nov. (VP)	Int. J. Syst. Evol. Microbiol. 55:1089*
[Ref.: #20215]	Species	Dinoroseobacter shibae	sp. nov. (VP)	Int. J. Syst. Evol. Microbiol. 55:1089*
[Ref.: #20215]	Full Scientific Name	Dinoroseobacter shibae Biebl et al. 2005

Information on morphological and physiological propertiesMorphology and physiology

[Ref.: #31424]	Gram stain	negative
[Ref.: #31424]	Cell length	0.65 µm
[Ref.: #31424]	Cell width	0.5 µm
[Ref.: #31424]	Cell shape	ovoid-shaped
[Ref.: #31424]	Motility	yes

[Ref.: #6375]

Incubation period

2-3 days

	Enzyme	Enzyme activity	EC number
[Ref.: #6375]	catalase	+	1.11.1.6
[Ref.: #6375]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #31424]	gelatinase	+

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31424]		NaCl		growth	1.0-7.0	%
[Ref.: #31424]		NaCl		optimum	4	%

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #6375]	6231	+	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31424]	Acetic acid	+	carbon source
[Ref.: #31424]	Citric acid	+	carbon source
[Ref.: #31424]	Fructose	+	carbon source
[Ref.: #31424]	Glucose	+	carbon source
[Ref.: #31424]	Glutamic acid	+	carbon source
[Ref.: #31424]	Glycerol	+	carbon source
[Ref.: #31424]	Lactic acid	+	carbon source
[Ref.: #31424]	Malic acid	+	carbon source
[Ref.: #31424]	Nitrate	+	reduction
[Ref.: #31424]	Pyruvic acid	+	carbon source
[Ref.: #31424]	Succinic acid	+	carbon source
[Ref.: #31424]	Tween 80	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

[Ref.: #31424]

Oxygen tolerance

aerobe

Information on culture and growth conditionsCulture and growth conditions

[Ref.: #6375]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514), 30°C

[Ref.: #6375]

Culture medium growth

[Ref.: #6375]

Culture medium link

medium recipe provided by DSMZ

		Temperature
[Ref.: #6375]	growth	30 ̊C
[Ref.: #31424]	growth	15-38 ̊C
[Ref.: #31424]	optimum	33 ̊C

[Ref.: #6375]	Temperature range	mesophilic
[Ref.: #31424]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31424]			optimum	7.75
[Ref.: #31424]			growth	6.2-8.9

Information on isolation source, the sampling and environmental conditionsIsolation, sampling and environmental information

[Ref.: #6375]

Sample type/isolated from

Culture of Prorocentrum lima (isolated at seaside near Vigo, Spain), dinoflagellate culture collection Helgoland

[Ref.: #6375]

Host species

Prorocentrum lima

[Ref.: #6375]

Geographic location (country and/or sea, region)

Helgoland

[Ref.: #6375]

Country

Germany

[Ref.: #6375]

Continent

Europe

Isolation sources categories

#Engineered	#Laboratory	#Lab enrichment
#Host	#Protozoa	#Dinoflagellate

What are isolation sources categories?

* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hostsApplication and interaction

[Ref.: #6375]

Biosafety level

Risk group (German classification)

Information on genomic background e.g. entries in nucleic sequence databassMolecular biology

[Ref.: #31424]

GC-content

64.8 mol%

Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence database	Sequence accession description	Sequence accession number	Sequence type	Sequence length (bp)	Associated NCBI tax ID
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	TPA: Dinoroseobacter shibae DFL 12 transfer-messenger mRNA Dinor_shiba_DFL12, two-piece tmRNA, Acceptor RNA	HG790644		77	398580	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	TPA: Dinoroseobacter shibae DFL 12 transfer-messenger mRNA Dinor_shiba_DFL12, two-piece tmRNA, Coding RNA	HG790645		209	398580	*
[Ref.: #20218]	INSDC Sequence	JP 2014508531-A/19: GENETICALLY MODIFIED CELL AND PROCESS FOR USE OF SAID CELL	HW575895		1458	398580	*
[Ref.: #20218]	INSDC Sequence	Sequence 32 from Patent WO2012064195	JC323548		1458	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Ala-GGC-1-1 gene	LK006326		76	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Ala-TGC-1-1 gene	LK006327		76	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Ala-TGC-1-2 gene	LK006328		76	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Arg-ACG-1-1 gene	LK006329		77	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Arg-CCG-1-1 gene	LK006330		77	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Arg-TCT-1-1 gene	LK006332		77	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Asn-GTT-1-1 gene	LK006333		75	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Asp-GTC-1-1 gene	LK006334		77	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Asp-GTC-1-2 gene	LK006335		77	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Cys-GCA-1-1 gene	LK006336		74	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Gln-TTG-1-1 gene	LK006337		75	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Glu-TTC-1-1 gene	LK006338		75	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Gly-GCC-1-1 gene	LK006340		75	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Gly-TCC-1-1 gene	LK006341		74	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-His-GTG-1-1 gene	LK006342		77	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Ile-GAT-1-1 gene	LK006343		77	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Ile-GAT-1-2 gene	LK006344		77	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Leu-CAA-1-1 gene	LK006345		87	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Leu-CAG-1-1 gene	LK006346		87	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Leu-GAG-1-1 gene	LK006347		87	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Leu-TAA-1-1 gene	LK006348		86	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Leu-TAG-1-1 gene	LK006349		85	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Lys-TTT-1-1 gene	LK006350		76	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Met-CAT-1-1 gene	LK006351		77	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Met-CAT-2-1 gene	LK006352		77	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Phe-GAA-1-1 gene	LK006353		75	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Pro-CGG-1-1 gene	LK006354		77	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Pro-GGG-1-1 gene	LK006355		77	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Pro-TGG-1-1 gene	LK006356		77	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Ser-CGA-1-1 gene	LK006357		90	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Ser-GCT-1-1 gene	LK006358		90	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Ser-GGA-1-1 gene	LK006359		90	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Ser-TGA-1-1 gene	LK006360		90	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Thr-CGT-1-1 gene	LK006361		76	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Thr-GGT-1-1 gene	LK006362		75	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Thr-TGT-1-1 gene	LK006363		76	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Trp-CCA-1-1 gene	LK006364		76	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Tyr-GTA-1-1 gene	LK006365		84	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Val-CAC-1-1 gene	LK006366		75	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Val-GAC-1-1 gene	LK006367		75	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-Val-TAC-1-1 gene	LK006368		76	398580	*
[Ref.: #20218]	INSDC Sequence	TPA: Dinoroseobacter shibae DFL 12 = DSM 16493 tRNA-iMet-CAT-1-1 gene	LK006369		77	398580	*
[Ref.: #6375]	GenBank Genome project data	Dinoroseobacter shibae DFL 12, complete genome	CP000830	WGS	3789584	398580
[Ref.: #31424]	GenBank/EMBL/DDBJ		AJ534211

Availability in culture collectionsStrain availability

[Ref.: #6375]

Culture collection no.

DSM 16493

[Ref.: #6375]

Strain history

<- H. Biebl <- M. Allgaier; DFL 12

[Ref.: #20218]

Associated Passport(s) in StrainInfo

387731

References

#6375

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16493

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#31424

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 ) - originally annotated from #27731 (see below)

#27731

IJSEM 1089 2005 ( DOI 10.1099/ijs.0.63511-0 )

* These References are textmined

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