Ahrensia kielensis B9 | Type strain | DSM 5890, ATCC 25656, IAM 12618, JCM 20689, CIP 106964, IFO 15762, LMG 133, NBRC 15762, NCIMB 2205 | BacDiveID:13649

Strain identifier

BacDive ID: 13649

Type strain:

Species: Ahrensia kielensis

Strain Designation: B9

Culture col. no.: DSM 5890, ATCC 25656, IAM 12618, JCM 20689, CIP 106964, IFO 15762, LMG 133, NBRC 15762, NCIMB 2205

Strain history: CIP <- 2001, D. Mazel, Inst. Pasteur, Paris, France <- 2001, IAM, Agrobacterium kielense <- ATCC <- R. Ahrens: strain B9

NCBI tax ID(s): 1120961 (strain), 76980 (species)

SI-ID 413

LMG 133, ATCC 25656, IAM 12618, LMG 8175, IFO 15762, NCMB 2205, CIP 106964, DSM 5890, NCIMB 2205, CECT 4270, NBRC 15762, JCM 20689

Section

Ahrensia kielensis B9 is a mesophilic, Gram-negative bacterium that was isolated from brackish water.

Gram-negative
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Ahrensiaceae*
	Genus	*Ahrensia*
	Species	**Ahrensia kielensis**
	Full Scientific Name (LPSN)	Ahrensia kielensis corrig. (ex Ahrens 1968) Uchino et al. 1999

Synonym

Ahrensia kieliense

Information on morphological and physiological properties Morphology

[Ref.: #120412]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.982

[Ref.: #120412]	Cell shape	rod-shaped

[Ref.: #120412]	Motility	yes

Multimedia content

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Information on culture and growth conditions Culture and growth conditions

Culture medium

MARINE AGAR (DSMZ Medium 123)

Culture medium growth

Culture medium link

Medium recipe at MediaDive

Culture medium composition

expand / minimize

Culture medium

Marine agar (MA)

Culture medium growth

Culture medium composition

expand / minimize

[Ref.: #120412]

Culture medium

CIP Medium 13

[Ref.: #120412]

Culture medium growth

[Ref.: #120412]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #2348]			growth	26 °C
[Ref.: #39626]			growth	25 °C
[Ref.: #67770]			growth	25 °C
[Ref.: #120412]			growth	5-37 °C
[Ref.: #120412]		no	growth	41 °C
[Ref.: #120412]		no	growth	45 °C

[Ref.: #2348]	Temperature range	mesophilic
[Ref.: #39626]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #120412]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.992

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #120412]		NaCl		growth	2-4	%
[Ref.: #120412]		NaCl	no	growth	0	%
[Ref.: #120412]		NaCl	no	growth	6	%
[Ref.: #120412]		NaCl	no	growth	8	%
[Ref.: #120412]		NaCl	no	growth	10	%

Observation

quinones: Q-10

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]		amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		D-fructose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		D-glucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #120412]		esculin ChEBI	-	hydrolysis
[Ref.: #68371]		esculin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #120412]		glucose ChEBI	+/-	degradation
[Ref.: #68371]		glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #120412]		hippurate ChEBI	-	hydrolysis
[Ref.: #68371]		inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #120412]		malonate ChEBI	-	assimilation
[Ref.: #68371]		maltose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #120412]		nitrate ChEBI	-	reduction
[Ref.: #120412]		nitrate ChEBI	-	respiration
[Ref.: #120412]		nitrite ChEBI	-	reduction
[Ref.: #68371]		Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		xylitol ChEBI	-	builds acid from	* from API 50CH acid
		Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #120412]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #120412]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #120412]		acetoin ChEBI	-
[Ref.: #120412]		glucose ChEBI		-

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #68382]		acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #120412]		alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]		alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]		alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]		alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]		alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]		alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]		alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #120412]		amylase	-
[Ref.: #120412]		beta-galactosidase	+	3.2.1.23
[Ref.: #68382]		beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]		beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]		beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #120412]		caseinase	-	3.4.21.50
[Ref.: #120412]		catalase	+	1.11.1.6
[Ref.: #68382]		cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]		esterase (C 4)	+		* from API zym
[Ref.: #68382]		esterase lipase (C 8)	+		* from API zym
[Ref.: #120412]		gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #120412]		gelatinase	-
[Ref.: #120412]		lecithinase	-
[Ref.: #68382]		leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #120412]		lipase	-
[Ref.: #68382]		lipase (C 14)	-		* from API zym
[Ref.: #120412]		lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]		N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]		naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #120412]		ornithine decarboxylase	-	4.1.1.17
[Ref.: #120412]		oxidase	+
[Ref.: #120412]		phenylalanine ammonia-lyase	+	4.3.1.24
[Ref.: #120412]		protease	-
[Ref.: #68382]		trypsin	+	3.4.21.4	* from API zym
[Ref.: #120412]		tryptophan deaminase	-
[Ref.: #120412]		tween esterase	-
[Ref.: #120412]		urease	-	3.5.1.5
[Ref.: #68382]		valine arylamidase	+		* from API zym
		Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #120412]	10773	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120412]	9950	-	+	+	+	-	+	+	+	+	-	+	-	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #2348]	Sample type/isolated from	brackish water
[Ref.: #2348]	Geographic location (country and/or sea, region)	Baltic Sea

[Ref.: #67770]	Sample type/isolated from	Brackish water
[Ref.: #67770]	Geographic location (country and/or sea, region)	Baltic Sea

[Ref.: #120412]	Sample type/isolated from	Environment, Brackish water
[Ref.: #120412]	Country	Baltic Sea
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Brackish

What are isolation sources categories?

Global distribution of 16S sequence D88524 (>99% sequence identity) for Ahrensia kielensis subclade from Microbeatlas

Aquatic Samples	758
Soil Samples	102
Animal Samples	73
Plant Samples	10
Total Samples	943

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2348]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120412]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #2348]	Agrobacterium kieliense gene for 16S rRNA, partial sequence	D88524	1407	ENA	76980 tax ID
[Ref.: #20218]	Ahrensia kielensis gene for 16S rRNA, partial sequence, strain: NBRC 15762	AB680960	1411	ENA	76980 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Ahrensia kielensis DSM 5890	GCA_000374465	scaffold	GenBank	1120961 tax ID
[Ref.: #66792]	Ahrensia kielensis DSM 5890	1120961.3	wgs	PATRIC	1120961 tax ID	*
[Ref.: #66792]	Ahrensia kielensis DSM 5890	2517572121	draft	JGI IMG/M	1120961 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Ahrensia kielensis DSM 5890 DSM 5890 NCBI: GCA_000374465.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Ahrensia kielensis DSM 5890 PATRIC: 1120961.3
[Ref.: #69480]	motile	yes	90.171	no
[Ref.: #69480]	flagellated	yes	59.304	no
[Ref.: #69480]	gram-positive	no	98.834	no
[Ref.: #69480]	anaerobic	no	97.929	no
[Ref.: #69480]	aerobic	yes	94.203	no
[Ref.: #69480]	halophile	yes	59.049	no
[Ref.: #69480]	spore-forming	no	97.461	no
[Ref.: #69480]	thermophile	no	96.536	yes
[Ref.: #69480]	glucose-util	yes	92.43	no
[Ref.: #69480]	glucose-ferment	no	90.944	no

Availability in culture collections External links

Culture collection no.

DSM 5890, ATCC 25656, IAM 12618, JCM 20689, CIP 106964, IFO 15762, LMG 133, NBRC 15762, NCIMB 2205

SI-ID 413

*

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Ahrensia marina sp. nov., a dimethylsulfoniopropionate-cleaving bacterium isolated from seawater, and emended descriptions of the genus Ahrensia and Ahrensia kielensis.	Liu J, Wang Y, Liu Y, Zhang XH	Int J Syst Evol Microbiol	10.1099/ijsem.0.000805	2015	*
Phylogeny	Pseudahrensia aquimaris gen. nov., sp. nov., isolated from seawater.	Jung YT, Park S, Lee JS, Oh TK, Yoon JH	Int J Syst Evol Microbiol	10.1099/ijs.0.034793-0	2011	*
Phylogeny	Reclassification of marine Agrobacterium species: Proposals of Stappia stellulata gen. nov., comb. nov., Stappia aggregata sp. nov., nom. rev., Ruegeria atlantica gen. nov., comb. nov., Ruegeria gelatinovora comb. nov., Ruegeria algicola comb. nov., and Ahrensia kieliense gen. nov., sp. nov., nom. rev.	Uchino Y, Hirata A, Yokota A, Sugiyama J	J Gen Appl Microbiol	10.2323/jgam.44.201	1998	*

References

#2348

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 5890

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#39626 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66793

Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#82832

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID413.1 )

#120412 Collection of Institut Pasteur ; Curators of the CIP; CIP 106964
* These data were automatically processed and therefore are not curated

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