Neorhizobium galegae (DSM 11542, ATCC 43677, CIP 105585)

Name: Neorhizobium galegae (DSM 11542, ATCC 43677, CIP 105585)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13626

Type strain

Culture col. no. DSM 11542 ATCC 43677 CIP 105585 JCM 20973 LMG 6214 7 more

NCBI tax ID(s) 399

Special Collections DSMZ CCUG JCM CIP

History <- ATCC <- K. Lindström, HAMBI 540 IAM 13631 <-- HAMBI 540 <-- K. Lindström gal1261. CIP <- 1998, Z. Terefework, Helsinki Univ., Helsinki, Finland: strain HAMBI 540 <- K. Lindström

Links

Neorhizobium galegae DSM 11542 is a Gram-negative, motile, rod-shaped bacterium that was isolated from root nodules from Galega orientalis.

Gram-negative motile rod-shaped genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 4763

LMG 6214,ATCC 43677,HAMBI 540,CCUG 27880,CIP 105585,DSM 11542,NCIMB 13151,IFO 14965,IAM 13631,CECT 4399,NBRC 14965,CBS 613.72,BCRC 15725,CCRC 15725,CCT 5090,CCT 4166,KACC 10639,JCM 20973,CFBP 7307

@ref 20215

Last LPSN update 2026-02-09 11:21:12

Domain Bacteria

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Hyphomicrobiales

Family Rhizobiaceae

Genus Neorhizobium

Species Neorhizobium galegae

Full scientific name Neorhizobium galegae (Lindström 1989) Mousavi et al. 2015

Synonyms (1)

Rhizobium galegae

BacDive ID	Other strains from **Neorhizobium galegae** (2)	Type strain
163043	N. galegae JCM 20974, HAMBI 490, IAM 13632
163077	N. galegae JCM 21073, HAMBI 1183, IAM 14208

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
119505	negative	rod-shaped
125438	negative		97.819
125439	negative		97.7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
4408	RHIZOBIUM MEDIUM (DSMZ Medium 98)	Medium recipe at MediaDive	Name: RHIZOBIUM MEDIUM (DSMZ Medium 98) Composition: air-dried garden soil 80.0 g/l Agar 15.0 g/l Mannitol 10.0 g/l Yeast extract 1.0 g/l Na2CO3 0.2 g/l Distilled water
34062	MEDIUM 1 - for Acetobacter, Azotobacter, Gluconobacter, Gluconacetobacter, Mesorhizodium ciceri and Pseudomonas doudoroffii		Distilled water make up to (1000.000 ml);Agar (15.000 g);Yeast extract (5.000 g);Peptone (3.000 g);Mannitol (25.000 g)
119505	CIP Medium 1	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
4408	positive	growth	30
34062	positive	growth	25
67770	positive	growth	25
119505	positive	growth	10-30
119505	negative	growth	37
119505	negative	growth	41
119505	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	96.3

Spore formation

@ref	Spore formation	Confidence
125438		93.119
125439		97.5

Halophily

@ref	Salt	Growth	Tested relation	Concentration
119505	NaCl	positive	growth	0 %
119505	NaCl		growth	2 %
119505	NaCl		growth	4 %
119505	NaCl		growth	6 %
119505	NaCl		growth	8 %
119505	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
119505	4853 ChEBI	esculin	+	hydrolysis
119505	606565 ChEBI	hippurate	-	hydrolysis
119505	17632 ChEBI	nitrate	-	reduction
119505	17632 ChEBI	nitrate	-	respiration
119505	16301 ChEBI	nitrite	+	reduction

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
119505	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
119505	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
119505	15688 ChEBI	acetoin	-
119505	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
119505	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
119505	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
119505	catalase	-	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	-		from API zym
119505	gamma-glutamyltransferase	-	2.3.2.2
119505	gelatinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
119505	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
119505	ornithine decarboxylase	-	4.1.1.17
119505	oxidase	+
119505	phenylalanine ammonia-lyase	+	4.3.1.24
68382	trypsin	+	3.4.21.4	from API zym
119505	tryptophan deaminase	-
119505	urease	+	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
119505	-	+	+	-	-	+	-	+	+	-	+	+	-	-	-	-	+	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
119505	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+	-	+	+	-	-	-	-	+	-	-	-	-	-	-	-	+	-	+	-	-	-	-	-	-	+	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root nodule

Isolation

@ref	Sample type	Host species	Country	Country ISO 3 Code	Continent
4408	root nodules from Galega orientalis	Galega orientalis	Finland	FIN	Europe
48993	Galega orientalis		Finland	FIN	Europe
67770	Galega orientalis	Galega orientalis	Finland	FIN	Europe
119505	Plant, Root nodule, Galega orientalis		Finland	FIN	Europe

Taxonmaps

69479

Global distribution of 16S sequence X67226 (>99% sequence identity) for Neorhizobium from Microbeatlas

Aquatic Samples	391
Soil Samples	1052
Animal Samples	295
Plant Samples	2045
Total Samples	3783

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
4408	1	Risk group (German classification) According to TRBA 466
119505	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	RG540_Ch_pA assembly for Neorhizobium galegae bv. orientalis str. HAMBI 540	complete	GCA_000731315	1028800.3	2585427634	1028800	99.23

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Rhizobium galegae gene for 16S rRNA, complete sequence, type strain: ATCC 43677	D11343	1466	399
20218	Rhizobium galegae strain HAMBI 540 16S-23S intergenic spacer, partial sequence	AF271646	1160	1028800
20218	R.galegae gene for 16S ribosomal RNA (partial)	X63823	260	1028800
20218	Rhizobium galegae 16S rRNA gene, partial, strain HAMBI540	Y12355	818	1028800
20218	Rhizobium galegae gene for 16S rRNA, strain: IAM 13631	D12793	1434	399
20218	Rhizobium galegae partial 16S rRNA gene, strain LMG 6214	X67226	1433	399
20218	Rhizobium galegae gene for 16S rRNA, partial sequence, strain: NBRC 14965	AB680726	1406	399

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Neorhizobium galegae bv. orientalis str. HAMBI 540
PATRIC: 1028800.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	96.30	no
125439	gram_stain	BacteriaNetⓘ	negative	97.70	no
125439	motility	BacteriaNetⓘ	yes	66.00	no
125439	spore_formation	BacteriaNetⓘ	no	97.50	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Neorhizobium galegae bv. orientalis str. HAMBI 540 HAMBI 540
NCBI: GCA_000731315.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	97.82	no
125438	anaerobic	anaerobicⓘ	no	95.80	no
125438	aerobic	aerobicⓘ	yes	85.51	no
125438	spore-forming	spore-formingⓘ	no	93.12	no
125438	thermophilic	thermophileⓘ	no	100.00	yes
125438	flagellated	motile2+ⓘ	yes	68.54	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Phenanthrene-Degrading and Nickel-Resistant Neorhizobium Strain Isolated from Hydrocarbon-Contaminated Rhizosphere of Medicago sativa L.	Golubev S, Rasterkovskaya M, Sungurtseva I, Burov A, Muratova A.	Microorganisms	10.3390/microorganisms12081586	2024
	Phylogeny and Phylogeography of Rhizobial Symbionts Nodulating Legumes of the Tribe Genisteae.	Stepkowski T, Banasiewicz J, Granada CE, Andrews M, Passaglia LMP.	Genes (Basel)	10.3390/genes9030163	2018
Pathogenicity	Guidelines for the description of rhizobial symbiovars.	Martinez-Romero E, Peix A, Hungria M, Mousavi SA, Martinez-Romero J, Young P.	Int J Syst Evol Microbiol	10.1099/ijsem.0.006373	2024
Genetics	Closed genomes of commercial inoculant rhizobia provide a blueprint for management of legume inoculation.	Kohlmeier MG, O'Hara GW, Ramsay JP, Terpolilli JJ.	Appl Environ Microbiol	10.1128/aem.02213-24	2025
Phylogeny	Presence of an Agrobacterium-Type Tumor-Inducing Plasmid in Neorhizobium sp. NCHU2750 and the Link to Phytopathogenicity.	Haryono M, Tsai YM, Lin CT, Huang FC, Ye YC, Deng WL, Hwang HH, Kuo CH.	Genome Biol Evol	10.1093/gbe/evy249	2018
Phylogeny	Novel, non-symbiotic isolates of Neorhizobium from a dryland agricultural soil.	Soenens A, Imperial J.	PeerJ	10.7717/peerj.4776	2018
Metabolism	Genome sequencing of two Neorhizobium galegae strains reveals a noeT gene responsible for the unusual acetylation of the nodulation factors.	Osterman J, Marsh J, Laine PK, Zeng Z, Alatalo E, Sullivan JT, Young JP, Thomas-Oates J, Paulin L, Lindstrom K.	BMC Genomics	10.1186/1471-2164-15-500	2014
	Redundancy and Specificity of Type VI Secretion vgrG Loci in Antibacterial Activity of Agrobacterium tumefaciens 1D1609 Strain.	Santos MNM, Cho ST, Wu CF, Chang CJ, Kuo CH, Lai EM.	Front Microbiol	10.3389/fmicb.2019.03004	2019
Genetics	Genomic and Biotechnological Characterization of the Heavy-Metal Resistant, Arsenic-Oxidizing Bacterium Ensifer sp. M14.	diCenzo GC, Debiec K, Krzysztoforski J, Uhrynowski W, Mengoni A, Fagorzi C, Gorecki A, Dziewit L, Bajda T, Rzepa G, Drewniak L.	Genes (Basel)	10.3390/genes9080379	2018
	Comparative in silico analysis of ftsZ gene from different bacteria reveals the preference for core set of codons in coding sequence structuring and secondary structural elements determination.	Pal A, Saha BK, Saha J.	PLoS One	10.1371/journal.pone.0219231	2019
Phylogeny	Rhizobium deserti sp. Nov Isolated from Biological Soil Crusts Collected at Mu Us Sandy Land, China.	Liu L, Liang L, Xu L, Chi M, Zhang X, Li L	Curr Microbiol	10.1007/s00284-019-01831-4	2019
Metabolism	Genomic features separating ten strains of Neorhizobium galegae with different symbiotic phenotypes.	Osterman J, Mousavi SA, Koskinen P, Paulin L, Lindstrom K	BMC Genomics	10.1186/s12864-015-1576-3	2015
	Persistence, population dynamics and competitiveness for nodulation of marker gene-tagged Rhizobium galegae strains in field lysimeters in the boreal climatic zone.	Pitkajarvi J, Rasanen LA, Langenskiold J, Wallenius K, Niemi M, Lindstrom K	FEMS Microbiol Ecol	10.1016/S0168-6496(03)00210-1	2003
	Stability of Markers Used for Identification of Two Rhizobium galegae Inoculant Strains after Five Years in the Field.	Lindstrom K, Lipsanen P, Kaijalainen S	Appl Environ Microbiol	10.1128/aem.56.2.444-450.1990	1990
Phylogeny	Neorhizobium xiangyangii sp. nov., isolated from a highland barley cultivation soil in Qamdo, Tibet.	Pan H, Zhou ZQ, He GW, Zhou J, Jin K, Liu HH, Wang C, Yang H, Yu YB, Tian Y	Arch Microbiol	10.1007/s00203-022-02961-3	2022
Phylogeny	Neorhizobium tomejilense sp. nov., first non-symbiotic Neorhizobium species isolated from a dryland agricultural soil in southern Spain.	Soenens A, Gomila M, Imperial J	Syst Appl Microbiol	10.1016/j.syapm.2018.09.001	2018
Phylogeny	Pseudorhizobium pelagicum gen. nov., sp. nov. isolated from a pelagic Mediterranean zone.	Kimes NE, Lopez-Perez M, Flores-Felix JD, Ramirez-Bahena MH, Igual JM, Peix A, Rodriguez-Valera F, Velazquez E	Syst Appl Microbiol	10.1016/j.syapm.2015.05.003	2015
Phylogeny	Rhizobium vignae sp. nov., a symbiotic bacterium isolated from multiple legume species.	Ren DW, Chen WF, Sui XH, Wang ET, Chen WX	Int J Syst Evol Microbiol	10.1099/ijs.0.023143-0	2010
Phylogeny	Rhizobium huautlense sp. nov., a symbiont of Sesbania herbacea that has a close phylogenetic relationship with Rhizobium galegae.	Wang ET, van Berkum P, Beyene D, Sui XH, Dorado O, Chen WX, Martinez-Romero E	Int J Syst Bacteriol	10.1099/00207713-48-3-687	1998

References

#4408	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 11542
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#34062	; Curators of the CIP;
#48993	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 27880
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#119505	Collection of Institut Pasteur ; Curators of the CIP; CIP 105585
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Neorhizobium galegae (DSM 11542, ATCC 43677, CIP 105585)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API zym

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000731315

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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