Name: Sinorhizobium americanum MexA1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13570

Type strain:

Species: Sinorhizobium americanum

Strain Designation: MexA1

Culture col. no.: DSM 15007, ATCC BAA-532, CIP 108390, CFNEI 156

Strain history: CIP <- 2004, E. Martinez-Romero, Centr. Investig. Fijacion Nitrogeno, Mexico Univ., Morelos, Mexico: strain CFNEI 156

NCBI tax ID(s): 194963 (species)

SI-ID 134445

ATCC BAA-532, CIP 108390, DSM 15007, LMG 22684

Section

Sinorhizobium americanum MexA1 is a mesophilic, Gram-negative, rod-shaped bacterium that was isolated from nitrogen fixing nodules of Acacia plants.

Gram-negative
rod-shaped
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Rhizobiaceae*
	Genus	Sinorhizobium
	Species	Sinorhizobium americanum
	Full Scientific Name (LPSN)	Sinorhizobium americanum corrig. Toledo et al. 2004

	Synonym	Sinorhizobium americanus
	Synonym	Ensifer americanum
	Synonym	**Ensifer americanus**

Information on morphological and physiological properties Morphology

[Ref.: #116327]	Gram stain	negative

[Ref.: #116327]	Cell shape	rod-shaped

[Ref.: #116327]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5662]

Culture medium

RHIZOBIUM MEDIUM (DSMZ Medium 98)

[Ref.: #5662]

Culture medium growth

[Ref.: #5662]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5662]

Culture medium composition

expand / minimize

[Ref.: #33237]

Culture medium

MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulum

[Ref.: #33237]

Culture medium growth

[Ref.: #33237]

Culture medium composition

expand / minimize

[Ref.: #116327]

Culture medium

CIP Medium 57

[Ref.: #116327]

Culture medium growth

[Ref.: #116327]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #5662]		growth	28-30 °C
[Ref.: #33237]		growth	30 °C
[Ref.: #116327]		growth	25-37 °C
[Ref.: #116327]	no	growth	10 °C
[Ref.: #116327]	no	growth	41 °C
[Ref.: #116327]	no	growth	45 °C

[Ref.: #5662]	Temperature range	mesophilic
[Ref.: #33237]	Temperature range	mesophilic
[Ref.: #116327]	Temperature range	mesophilic
[Ref.: #116327]	Temperature range	psychrophilic
[Ref.: #116327]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

	Salt	Tested relation		Salt conc.
[Ref.: #116327]	NaCl		growth	0	%
[Ref.: #116327]	NaCl	no	growth	2	%
[Ref.: #116327]	NaCl	no	growth	4	%
[Ref.: #116327]	NaCl	no	growth	6	%
[Ref.: #116327]	NaCl	no	growth	8	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #116327]	citrate ChEBI	-	carbon source
[Ref.: #116327]	esculin ChEBI	-	hydrolysis
[Ref.: #116327]	hippurate ChEBI	-	hydrolysis
[Ref.: #116327]	nitrate ChEBI	+	reduction
[Ref.: #116327]	nitrite ChEBI	-	reduction

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #116327]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #116327]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #116327]		acetoin ChEBI	-
[Ref.: #116327]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	+	3.2.1.24	* from API zym
[Ref.: #116327]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #116327]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #116327]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #116327]	gelatinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #116327]	oxidase	-
[Ref.: #116327]	phenylalanine ammonia-lyase	+	4.3.1.24
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #116327]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #116327]	2730	not determinedn.d.	+	+	-	+	-	-	+	+	-	-	+	+	+	+	+	-	-	-	-	+	-	-	-	+	+	-	+	-	-	-	-	+	+	+	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	+	+	-	+	-	+	-	-	-	+	+	-	-	-	-	-	-	-	+	+	+	+	-	+	-	+	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116327]	11142	-	+	+	+	-	+	-	-	+	-	+	+	-	+	-	+	+	+	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5662]	Sample type/isolated from	nitrogen fixing nodules of Acacia plants
[Ref.: #5662]	Host species	Acacia
[Ref.: #5662]	Geographic location (country and/or sea, region)	Morelos, Sierra de Huautla
[Ref.: #5662]	Country	Mexico
[Ref.: #5662]	Country ISO 3 Code	MEX
[Ref.: #5662]	Continent	North America
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Shrub (Scrub)
#Host Body-Site	#Plant	#Root nodule

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AF506513 (>99% sequence identity) for Rhizobiaceae from Microbeatlas

Aquatic Samples	1562
Soil Samples	5353
Animal Samples	1094
Plant Samples	1478
Total Samples	9487

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5662]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116327]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5662]	Sinorhizobium americanum strain CFNEI 156 16S ribosomal RNA gene, partial sequence	AF506513	1437	ENA	194963 tax ID

Availability in culture collections External links

[Ref.: #5662]

Culture collection no.

DSM 15007, ATCC BAA-532, CIP 108390, CFNEI 156

[Ref.: #82757]

SI-ID 134445

Literature:

	Title	Authors	Journal	DOI	Year
Phylogeny	Sinorhizobium americanum symbiovar mediterranense is a predominant symbiont that nodulates and fixes nitrogen with common bean (Phaseolus vulgaris L.) in a Northern Tunisian field.	Mnasri B, Saidi S, Chihaoui SA, Mhamdi R	Syst Appl Microbiol	10.1016/j.syapm.2012.04.003	2012	*
	Exo-Metabolites of Phaseolus vulgaris-Nodulating Rhizobial Strains.	Montes-Grajales D, Esturau-Escofet N, Esquivel B, Martinez-Romero E	Metabolites	10.3390/metabo9060105	2019	*

References

#5662

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15007

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#33237 ; Curators of the CIP;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#82757

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID134445.1 )

#116327 Collection of Institut Pasteur ; Curators of the CIP; CIP 108390
* These data were automatically processed and therefore are not curated

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