Name: Ensifer sojae DSM 26426
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13562

Type strain:

Species: Ensifer sojae

Culture col. no.: DSM 26426, CCBAU 05684, HAMBI 3098, LMG 25493

Strain history: <- LMG <- X. Sui <- Q. Li, China Agric. Univ., College Biol. Sci., Beijing

NCBI tax ID(s): 716928 (strain), 716925 (species)

SI-ID 368414

LMG 25493, HAMBI 3098, DSM 26426

Section

Ensifer sojae DSM 26426 is a mesophilic, motile bacterium that was isolated from effective nodules of Glycine max.

motile
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Rhizobiaceae*
	Genus	*Ensifer*
	Species	**Ensifer sojae**
	Full Scientific Name (LPSN)	Ensifer sojae Li et al. 2011

Synonym

Sinorhizobium sojae

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.992

[Ref.: #69480]	Motility	yes Confidence in %90.207

[Ref.: #18216]

Incubation period

2-3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #18216]

Culture medium

RHIZOBIUM MEDIUM (DSMZ Medium 98)

[Ref.: #18216]

Culture medium growth

[Ref.: #18216]

Culture medium link

Medium recipe at MediaDive

[Ref.: #18216]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #18216]			growth	30 °C

[Ref.: #18216]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.997

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	+	3.2.1.21	* from API 20NE
[Ref.: #18216]	catalase	-	1.11.1.6
[Ref.: #18216]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #68369]	gelatinase	-		* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #18216]	23936	-	-	-	-	+/-	+	-	+	+	+	-	+	+	+	+	-	-	+/-	-	-	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #18216]	Sample type/isolated from	effective nodules of Glycine max
[Ref.: #18216]	Host species	Glycine max
[Ref.: #18216]	Geographic location (country and/or sea, region)	Hebei
[Ref.: #18216]	Country	China
[Ref.: #18216]	Country ISO 3 Code	CHN
[Ref.: #18216]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root nodule

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence GU593061 (>99% sequence identity) for Rhizobiaceae from Microbeatlas

Aquatic Samples	1562
Soil Samples	5353
Animal Samples	1094
Plant Samples	1478
Total Samples	9487

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18216]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #18216]	Ensifer sojae CCBAU 05684 16S ribosomal RNA gene, partial sequence	GU593061	1351	ENA	716928 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Ensifer sojae CCBAU 05684	2802429268	complete	JGI IMG/M	716928 tax ID	*
[Ref.: #66792]	Sinorhizobium sojae CCBAU 05684	GCA_000261485	contig	GenBank	716928 tax ID	*
[Ref.: #66792]	Ensifer sojae CCBAU 05684	716928.14	plasmid	PATRIC	716928 tax ID	*
[Ref.: #66792]	Ensifer sojae CCBAU 05684	716928.13	plasmid	PATRIC	716928 tax ID	*
[Ref.: #66792]	Ensifer sojae CCBAU 05684	716928.12	complete	PATRIC	716928 tax ID	*
[Ref.: #66792]	Ensifer sojae CCBAU 05684	716928.3	wgs	PATRIC	716928 tax ID	*

[Ref.: #18216]

GC-content

60.9 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Ensifer sojae CCBAU 05684 CCBAU 05684 NCBI: GCA_002288525.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Ensifer sojae CCBAU 05684 CCBAU 05684 NCBI: GCA_002288525.1
[Ref.: #69480]	flagellated	no	77.963	no
[Ref.: #69480]	gram-positive	no	98.601	no
[Ref.: #69480]	anaerobic	no	98.607	no
[Ref.: #69480]	aerobic	yes	91.406	no
[Ref.: #69480]	halophile	no	83.124	no
[Ref.: #69480]	spore-forming	no	96.041	no
[Ref.: #69480]	glucose-util	yes	92.286	no
[Ref.: #69480]	thermophile	no	98.502	yes
[Ref.: #69480]	motile	yes	89.539	no
[Ref.: #69480]	glucose-ferment	no	89.928	yes

Availability in culture collections External links

[Ref.: #18216]

Culture collection no.

DSM 26426, CCBAU 05684, HAMBI 3098, LMG 25493

[Ref.: #82750]

SI-ID 368414

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Ensifer alkalisoli sp. nov. isolated from root nodules of Sesbania cannabina grown in saline-alkaline soils.	Li Y, Yan J, Yu B, Wang ET, Li X, Yan H, Liu W, Xie Z	Int J Syst Evol Microbiol	10.1099/ijsem.0.001510	2016	*
Phylogeny	Ensifer sojae sp. nov., isolated from root nodules of Glycine max grown in saline-alkaline soils.	Li QQ, Wang ET, Chang YL, Zhang YZ, Zhang YM, Sui XH, Chen WF, Chen WX	Int J Syst Evol Microbiol	10.1099/ijs.0.025049-0	2010	*
	[Biological characteristics of bacteriophages infecting three typic rhizobia of legume].	Liu JJ, Liu ZX, Yu H, Yao Q, Yu ZH, Wang GH	Ying Yong Sheng Tai Xue Bao	10.13287/j.1001-9332.201908.029	2019	*

References

#18216

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 26426

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#82750

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID368414.1 )

* These data were automatically processed and therefore are not curated

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Ensifer sojae CCBAU 05684 CCBAU 05684

Ensifer sojae CCBAU 05684 CCBAU 05684

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