Name: Halobacillus salsuginis DSM 21185
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1356

Type strain:

Species: Halobacillus salsuginis

Culture col. no.: DSM 21185, KCTC 13236, JSM 078133

Strain history: <- X-L Cui, Yunnan Inst., China

NCBI tax ID(s): 578693 (species)

SI-ID 400176

DSM 21185

Section

Halobacillus salsuginis DSM 21185 is an aerobe, moderately halophilic, spore-forming bacterium that forms circular colonies and was isolated from From a subterranean brine from the Xiangli Salt Mine.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
aerobe
mesophilic
moderately halophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Halobacillus*
	Species	**Halobacillus salsuginis**
	Full Scientific Name (LPSN)	Halobacillus salsuginis Chen et al. 2009

Information on morphological and physiological properties Morphology

[Ref.: #22993]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive

[Ref.: #22993]	Cell length	1.5-3.0 µm

[Ref.: #22993]	Cell width	0.5-0.9 µm

[Ref.: #22993]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #22993]	Motility	yes
[Ref.: #67771]	Motility	yes

[Ref.: #22993]	Flagellum arrangement	peritrichous
[Ref.: #67771]	Flagellum arrangement	peritrichous

[Ref.: #22993]	Colony size	1.0-2.0 mm
[Ref.: #22993]	Colony color	cream
[Ref.: #22993]	Colony shape	circular
[Ref.: #22993]	Incubation period	3-5 days
[Ref.: #22993]	Cultivation medium used	MA5

Information on culture and growth conditions Culture and growth conditions

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Culture medium

MODIFIED BACTO MARINE BROTH (DSMZ Medium 514d)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #15493]

Culture medium composition

expand / minimize

[Ref.: #22993]

Culture medium

MA5

[Ref.: #22993]

Culture medium growth

		Temperature
[Ref.: #15493]	growth	28 °C
[Ref.: #22993]	growth	10.0-45.0 °C
[Ref.: #22993]	optimum	30.0 °C
[Ref.: #67771]	growth	30 °C

[Ref.: #15493]	Temperature range	mesophilic
[Ref.: #22993]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #22993]			optimum	7.5
[Ref.: #22993]			growth	6.0-10.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #22993]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #22993]	Spore description	ellipsoidal, subterminal,in swollen sporangia
[Ref.: #22993]	Type of spore	endospore
[Ref.: #22993]	Ability of spore formation	yes
[Ref.: #67771]	Type of spore	endospore
[Ref.: #67771]	Ability of spore formation	yes

[Ref.: #22993]

Halophily / tolerance level

moderately halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #22993]		NaCl		growth	1.0-23.0	%(w/v)
[Ref.: #22993]		NaCl		optimum	5.0-10.0	%(w/v)

[Ref.: #67771]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #22993]	casein	-	hydrolysis
[Ref.: #22993]	cellobiose ChEBI	-	builds acid from
[Ref.: #22993]	cellulose ChEBI	-	hydrolysis
[Ref.: #22993]	D-arabitol ChEBI	+	builds acid from
[Ref.: #22993]	D-fructose ChEBI	-	builds acid from
[Ref.: #22993]	D-galactose ChEBI	-	builds acid from
[Ref.: #22993]	D-glucose ChEBI	+	builds acid from
[Ref.: #22993]	D-mannitol ChEBI	-	builds acid from
[Ref.: #22993]	D-mannose ChEBI	-	builds acid from
[Ref.: #22993]	D-ribose ChEBI	-	builds acid from
[Ref.: #22993]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #22993]	D-xylose ChEBI	-	builds acid from
[Ref.: #22993]	dna ChEBI	+	hydrolysis
[Ref.: #22993]	esculin ChEBI	+	hydrolysis
[Ref.: #22993]	galactitol ChEBI	-	builds acid from
[Ref.: #22993]	gelatin ChEBI	+	hydrolysis
[Ref.: #22993]	glucose ChEBI	-	fermentation
[Ref.: #22993]	glycerol ChEBI	-	builds acid from
[Ref.: #22993]	hypoxanthine ChEBI	-	hydrolysis
[Ref.: #22993]	L-arabinose ChEBI	-	builds acid from
[Ref.: #22993]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #22993]	lactose ChEBI	-	builds acid from
[Ref.: #22993]	maltose ChEBI	+	builds acid from
[Ref.: #22993]	melezitose ChEBI	-	builds acid from
[Ref.: #22993]	melibiose ChEBI	-	builds acid from
[Ref.: #22993]	myo-inositol ChEBI	-	builds acid from
[Ref.: #22993]	nitrate ChEBI	+	reduction
[Ref.: #22993]	nitrite ChEBI	-	reduction
[Ref.: #22993]	raffinose ChEBI	-	builds acid from
[Ref.: #22993]	ribitol ChEBI	-	builds acid from
[Ref.: #22993]	salicin ChEBI	-	builds acid from
[Ref.: #22993]	starch ChEBI	+	hydrolysis
[Ref.: #22993]	sucrose ChEBI	+	builds acid from
[Ref.: #22993]	trehalose ChEBI	+	builds acid from
[Ref.: #22993]	tween 20 ChEBI	-	hydrolysis
[Ref.: #22993]	tween 40 ChEBI	+	hydrolysis
[Ref.: #22993]	tween 60 ChEBI	-	hydrolysis
[Ref.: #22993]	tween 80 ChEBI	-	hydrolysis
[Ref.: #22993]	tyrosine ChEBI	-	hydrolysis
[Ref.: #22993]	xanthine ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #22993]	ampicillin ChEBI	yes	30	µg (disc)
[Ref.: #22993]	carbenicillin ChEBI	yes	30	µg (disc)
[Ref.: #22993]	chloramphenicol ChEBI	yes	30	µg (disc)
[Ref.: #22993]	gentamicin ChEBI	yes	10	µg (disc)
[Ref.: #22993]	kanamycin ChEBI	yes	30	µg (disc)
[Ref.: #22993]	lincomycin ChEBI	yes	2	µg (disc)
[Ref.: #22993]	nalidixic acid ChEBI	no			yes	20	µg (disc)
[Ref.: #22993]	nitrofurantoin ChEBI	no			yes	30	µg (disc)
[Ref.: #22993]	novobiocin ChEBI	no			yes	30	µg (disc)
[Ref.: #22993]	polymyxin b ChEBI	no			yes	30	µg (disc)
[Ref.: #22993]	rifampicin ChEBI	yes	5	µg (disc)
[Ref.: #22993]	streptomycin ChEBI	yes	10	µg (disc)
[Ref.: #22993]	tetracycline ChEBI	no			yes	30	µg (disc)
[Ref.: #22993]	tobramycin ChEBI	no			yes	10	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #22993]	acetoin ChEBI	no
[Ref.: #22993]	hydrogen sulfide ChEBI	no
[Ref.: #22993]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #22993]		acetoin ChEBI	-
[Ref.: #22993]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #22993]	catalase	+	1.11.1.6
[Ref.: #22993]	cytochrome oxidase	+	1.9.3.1
[Ref.: #22993]	lysine decarboxylase	-	4.1.1.18
[Ref.: #22993]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #22993]	phenylalanine deaminase	+	4.3.1.5
[Ref.: #22993]	urease	-	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67771]	Sample type/isolated from	From a subterranean brine from the Xiangli Salt Mine
[Ref.: #67771]	Geographic location (country and/or sea, region)	Hunan Province
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia

[Ref.: #22993]	Sample type/isolated from	subterranean brine sample
[Ref.: #22993]	Geographic location (country and/or sea, region)	Xiangli Salt Mine,Hunan Province
[Ref.: #22993]	Country	China
[Ref.: #22993]	Country ISO 3 Code	CHN
[Ref.: #22993]	Continent	Asia
[Ref.: #22993]	Enrichment culture	Difco marine agar 2216 (MA) supplemented with 5 % (w/v) NaCl
[Ref.: #22993]	Enrichment culture duration	14 days
[Ref.: #22993]	Enrichment culture temperature	30.0 ̊C

[Ref.: #15493]	Sample type/isolated from	subterranean brine
[Ref.: #15493]	Geographic location (country and/or sea, region)	Hunan Province, Xiangli Salt Mine
[Ref.: #15493]	Country	China
[Ref.: #15493]	Country ISO 3 Code	CHN
[Ref.: #15493]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	-
#Condition	#Saline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence FJ456889 (>99% sequence identity) for Halobacillus from Microbeatlas

Aquatic Samples	147
Soil Samples	114
Animal Samples	43
Plant Samples	19
Total Samples	323

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15493]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15493]	UNVERIFIED: Halobacillus salsuginis strain JSM078133 16S ribosomal RNA gene, partial sequence	FJ456889	1541	ENA	578693 tax ID

[Ref.: #15493]	GC-content	42.2 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67771]	GC-content	42.2 mol%

Availability in culture collections External links

[Ref.: #15493]

Culture collection no.

DSM 21185, KCTC 13236, JSM 078133

[Ref.: #71007]

SI-ID 400176

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Halobacillus andaensis sp. nov., a moderately halophilic bacterium isolated from saline and alkaline soil.	Wang K, Zhang L, Yang Y, Pan Y, Meng L, Liu H, Hong S, Huang H, Jiang J	Int J Syst Evol Microbiol	10.1099/ijs.0.000198	2015	*
Phylogeny	Halobacillus salsuginis sp. nov., a moderately halophilic bacterium from a subterranean brine.	Chen YG, Zhang YQ, Liu ZX, Zhuang DC, Klenk HP, Tang SK, Cui XL, Li WJ	Int J Syst Evol Microbiol	10.1099/ijs.0.010801-0	2009	*

References

#15493

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21185

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#22993

Yi-Guang Chen,Yu-Qin Zhang,Zhu-Xiang Liu,Da-Chun Zhuang,Hans-Peter Klenk,Shu-Kun Tang,Xiao-Long Cui,Wen-Jun Li: Halobacillus salsuginis sp. nov., a moderately halophilic bacterium from a subterranean brine. IJSEM 59: 2505 - 2509 2009 ( DOI 10.1099/ijs.0.010801-0 , PubMed 19622644 )

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#71007

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID400176.1 )

* These data were automatically processed and therefore are not curated

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