Lactobacillus crispatus (CIP 103603, NCTC 4505, NCIMB 4505)

Name: Lactobacillus crispatus (CIP 103603, NCTC 4505, NCIMB 4505)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 135309

Type strain

Culture col. no. CIP 103603 NCTC 4505 NCIMB 4505 CCUG 42897 LMG 11440 1 more

NCBI tax ID(s) 47770

Special Collections CCUG CIP

History CIP <- 1992, F. Gasser, Inst. Pasteur, Paris, France, Lactobacillus acidophilus, group A2 <- J.L. Johnson, Blacksburg, USA: strain VPI 11086 <- NCIB <- NCTC <- R. Cruickshank

Links

Lactobacillus crispatus CIP 103603 is an anaerobe, mesophilic, Gram-positive prokaryote that was isolated from Human vagina,pregnant woman.

Gram-positive rod-shaped anaerobe mesophilic genome sequence

Name and taxonomic classification

SI-ID 3274

LMG 11440,NCFB 5,NCIMB 4505,NCTC 4505,CIP 103603,NCIB 4505,NCDO5,CCUG 42897,NCCB 98068,LMD 98.68

@ref 20215

Last LPSN update 2025-12-16 10:02:16

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Lactobacillus

Species Lactobacillus crispatus

Full scientific name Lactobacillus crispatus (Brygoo and Aladame 1953) Moore and Holdeman 1970 (Approved Lists 1980)

Synonyms (1)

"Eubacterium crispatum"

BacDive ID	Other strains from **Lactobacillus crispatus** (34)	Type strain
6442	L. crispatus DSM 20584, ATCC 33820, CCUG 30722, JCM 1185, ... (type strain)
6441	L. crispatus A 12, DSM 20356, JCM 2123, CIP 105000, LMG ...
135308	L. crispatus CIP 103602, CG 97, VPI 1784
135310	L. crispatus VJ-A, CIP 103604, VPI 6317
135311	L. crispatus CIP 103605, VPI 7635
135475	L. crispatus F3, CIP 105003, JCM 8780, LMG 18201
136226	L. crispatus A11, CIP 103608
137068	L. crispatus 58-098-135, CIP 104459
138142	L. crispatus F5, CIP 105004, JCM 8781, CCUG 31411, LMG ...
138805	L. crispatus A5, CIP 103606, VPI 11697
139286	L. crispatus Sp34, CIP 105001, JCM 5808, KCTC 3176, LMG ...
139287	L. crispatus CIP 105002, JCM 5810, KCTC 3178, LMG 18191
144917	L. crispatus CCUG 27076 A
145367	L. crispatus CCUG 28280
146793	L. crispatus CCUG 32128
148293	L. crispatus CCUG 35170
149929	L. crispatus CCUG 38700
150970	L. crispatus CCUG 42898, NCIMB 4504, NCTC 4504, LMG 12005
151041	L. crispatus CCUG 43131
151380	L. crispatus CCUG 44015
151479	L. crispatus CCUG 44128
152257	L. crispatus CCUG 46228
152773	L. crispatus CCUG 47593
155480	L. crispatus CCUG 57836
160075	L. crispatus Cla-CZ-47, DSM 108970
160903	L. crispatus JCM 1030, KCTC 3147, LMG 12003, NCFB 2172, ...
162742	L. crispatus JCM 2009, ATCC 33197, LMG 12004, VPI 7635
165301	L. crispatus JCM 5806, KCTC 3174, LMG 18187
165564	L. crispatus JCM 7696
165565	L. crispatus JCM 7697
165568	L. crispatus JCM 7702
165950	L. crispatus JCM 8778, LMG 18199
165951	L. crispatus JCM 8779, LMG 18200
172658	L. crispatus 2082, CRBIP24.77

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
34393	positive	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Growth	Composition	Medium link
34393	MEDIUM 40- for Lactobacillus and Leuconostoc		Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
34393	CIP Medium 40			Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
34393	positive	growth	37	mesophilic
34393	positive	growth	22-45
34393	negative	growth	10
34393	negative	growth	15
55597	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
55597	anaerobe
55597	microaerophile
34393	facultative anaerobe

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68381	40585 ChEBI	alpha-cyclodextrin	-	builds acid from	from API rID32STR
68371	27613 ChEBI	amygdalin	+	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	+	builds acid from	from API 50CH acid
68381	29016 ChEBI	arginine	-	hydrolysis	from API rID32STR
68380	29016 ChEBI	arginine	-	hydrolysis	from API rID32A
68371	17057 ChEBI	cellobiose	+	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68381	18333 ChEBI	D-arabitol	-	builds acid from	from API rID32STR
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
68379	17634 ChEBI	D-glucose	+	fermentation	from API Coryne
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68381	16899 ChEBI	D-mannitol	-	builds acid from	from API rID32STR
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68380	16024 ChEBI	D-mannose	+	fermentation	from API rID32A
68381	16988 ChEBI	D-ribose	-	builds acid from	from API rID32STR
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68381	16443 ChEBI	D-tagatose	-	builds acid from	from API rID32STR
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68379	4853 ChEBI	esculin	+	hydrolysis	from API Coryne
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68379	5291 ChEBI	gelatin	-	hydrolysis	from API Coryne
68371	28066 ChEBI	gentiobiose	+	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68381	28087 ChEBI	glycogen	-	builds acid from	from API rID32STR
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
68381	606565 ChEBI	hippurate	-	hydrolysis	from API rID32STR
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68381	30849 ChEBI	L-arabinose	-	builds acid from	from API rID32STR
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68380	29985 ChEBI	L-glutamate	-	degradation	from API rID32A
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68381	17716 ChEBI	lactose	-	builds acid from	from API rID32STR
68379	17716 ChEBI	lactose	+	fermentation	from API Coryne
68381	17306 ChEBI	maltose	+	builds acid from	from API rID32STR
68379	17306 ChEBI	maltose	+	fermentation	from API Coryne
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68381	6731 ChEBI	melezitose	-	builds acid from	from API rID32STR
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68381	28053 ChEBI	melibiose	-	builds acid from	from API rID32STR
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68381	320055 ChEBI	methyl beta-D-glucopyranoside	-	builds acid from	from API rID32STR
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
68379	17632 ChEBI	nitrate	-	reduction	from API Coryne
34393	17632 ChEBI	nitrate	-	reduction
34393	17632 ChEBI	nitrate	+	respiration
68380	17632 ChEBI	nitrate	-	reduction	from API rID32A
34393	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68381	27941 ChEBI	pullulan	-	builds acid from	from API rID32STR
68381	16634 ChEBI	raffinose	-	builds acid from	from API rID32STR
68380	16634 ChEBI	raffinose	+	fermentation	from API rID32A
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	+	builds acid from	from API 50CH acid
68381	30911 ChEBI	sorbitol	-	builds acid from	from API rID32STR
68381	17992 ChEBI	sucrose	+	builds acid from	from API rID32STR
68379	17992 ChEBI	sucrose	+	fermentation	from API Coryne
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68381	27082 ChEBI	trehalose	+	builds acid from	from API rID32STR
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68380	27897 ChEBI	tryptophan	-	energy source	from API rID32A
68381	16199 ChEBI	urea	-	hydrolysis	from API rID32STR
68380	16199 ChEBI	urea	-	hydrolysis	from API rID32A
68379	16199 ChEBI	urea	-	hydrolysis	from API Coryne
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68381	15688 ChEBI	acetoin		from API rID32STR
68380	35581 ChEBI	indole		from API rID32A

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68381	15688 ChEBI	acetoin	-		from API rID32STR
34393	15688 ChEBI	acetoin	-
68380	35581 ChEBI	indole		-	from API rID32A

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68380	alanine arylamidase	+	3.4.11.2	from API rID32A
68381	Alanyl-Phenylalanyl-Proline arylamidase	+		from API rID32STR
34393	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68379	alkaline phosphatase	-	3.1.3.1	from API Coryne
68381	alkaline phosphatase	-	3.1.3.1	from API rID32STR
68380	alkaline phosphatase	-	3.1.3.1	from API rID32A
68380	alpha-arabinosidase	-	3.2.1.55	from API rID32A
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68380	alpha-fucosidase	-	3.2.1.51	from API rID32A
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68381	alpha-galactosidase	-	3.2.1.22	from API rID32STR
68380	alpha-galactosidase	+	3.2.1.22	from API rID32A
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	-	3.2.1.20	from API Coryne
68380	alpha-glucosidase	+	3.2.1.20	from API rID32A
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68381	arginine dihydrolase	-	3.5.3.6	from API rID32STR
68380	arginine dihydrolase	-	3.5.3.6	from API rID32A
68381	beta-galactosidase	+	3.2.1.23	from API rID32STR
68380	beta-galactosidase	+	3.2.1.23	from API rID32A
68379	beta-galactosidase	+	3.2.1.23	from API Coryne
68380	beta-Galactosidase 6-phosphate	-		from API rID32A
68382	beta-glucosidase	+	3.2.1.21	from API zym
68380	beta-glucosidase	+	3.2.1.21	from API rID32A
68379	beta-glucosidase	+	3.2.1.21	from API Coryne
68381	beta-glucosidase	-	3.2.1.21	from API rID32STR
68381	beta-glucuronidase	-	3.2.1.31	from API rID32STR
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68380	beta-glucuronidase	-	3.2.1.31	from API rID32A
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
68381	beta-mannosidase	-	3.2.1.25	from API rID32STR
34393	catalase	-	1.11.1.6
68379	catalase	-	1.11.1.6	from API Coryne
68382	esterase lipase (C 8)	-		from API zym
68379	gelatinase	-		from API Coryne
68380	glutamate decarboxylase	-	4.1.1.15	from API rID32A
68380	glutamyl-glutamate arylamidase	-		from API rID32A
68380	glycin arylamidase	-		from API rID32A
68381	glycyl tryptophan arylamidase	-		from API rID32STR
68380	histidine arylamidase	-		from API rID32A
68380	L-arginine arylamidase	+		from API rID32A
68382	leucine arylamidase	+	3.4.11.1	from API zym
68380	leucine arylamidase	+	3.4.11.1	from API rID32A
68380	leucyl glycin arylamidase	-	3.4.11.1	from API rID32A
68382	lipase (C 14)	-		from API zym
34393	lysine decarboxylase	-	4.1.1.18
68381	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API rID32STR
68380	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API rID32A
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
34393	ornithine decarboxylase	-	4.1.1.17
34393	oxidase	-
68380	phenylalanine arylamidase	+		from API rID32A
68380	proline-arylamidase	-	3.4.11.5	from API rID32A
68379	pyrazinamidase	-	3.5.1.B15	from API Coryne
68381	pyrrolidonyl arylamidase	-	3.4.19.3	from API rID32STR
68380	pyrrolidonyl arylamidase	-	3.4.19.3	from API rID32A
68379	pyrrolidonyl arylamidase	-	3.4.19.3	from API Coryne
68380	serine arylamidase	+		from API rID32A
68382	trypsin	-	3.4.21.4	from API zym
68380	tryptophan deaminase	-	4.1.99.1	from API rID32A
68380	tyrosine arylamidase	+		from API rID32A
68381	urease	-	3.5.1.5	from API rID32STR
68380	urease	-	3.5.1.5	from API rID32A
68379	urease	-	3.5.1.5	from API Coryne

Fatty acid profile

Metadata FA analysis

@ref

55597

Fatty acid	Percentage	ECL
C18:2 ω6,9c/C18:0 ANTE	34.8	17.724
C18:1 ω9c	22.8	17.769
C16:0	21.2	16
C18:0	16.3	18
C14:0	2	14
C16:1 ω7c	1.7	15.819
C19:0 CYCLO ω9c	1.3	18.87

Metadata FA analysis

@ref

55597

Fatty acid	Percentage	ECL
C18:2 ω6,9c/C18:0 ANTE	29.6	17.724
C16:0	24.2	16
C18:1 ω9c	21	17.769
C18:0	18.8	18
C16:1 ω7c	4	15.819
C14:0	2.5	14

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
55597	-	-	-	-	-	+	-	-	+	-	-	-	+	-	-	-	+	+	+	-	-

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
55597	-	-	-	-	-	+	-	-	-	-	+	+	+	+	-	+	+	-	-	-
34393	-	-	+	-	-	+	+	+	-	-	+	+	+	-	-	+	+	+	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
55597	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	+	+	+	+	+	+	+	+	+	+	+	+	-	-	+	+	+	-	+	+	-	-	-	-	-	-	-	-	-
34393	not determinedn.d.	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	-	+	+	+	+	+	+	+	-	-	+	+	-	-	-	-	-	-	+	-	-	+	-	-	-	-	-	-	-

API rID32A

@ref	URE	ADH (Arg)	alpha GAL	beta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha GLU	beta GLU	alpha ARA	beta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	MNE	RAF	GDC	alpha FUC	Reduction of nitrateNIT	IND	PAL	L-arginine arylamidaseArgA	ProA	LGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	PyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
55597	-	-	+	+	-	+	+	-	-	-	+	+	-	-	-	-	-	+	-	-	+	+	-	+	+	-	-	-	+

API rID32STR

@ref	ADH (Arg)	beta GLU	beta GAR	beta GUR	alpha GAL	PAL	RIB	MAN	SOR	LAC	TRE	RAF	SAC	LARA	DARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	HIP	GLYG	PUL	MAL	MEL	MLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	TAG	beta MAN	URE
55597	-	-	+	-	-	-	-	-	-	-	+	-	+	-	-	-	-	+	+	-	-	-	-	-	-	+	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Human	#Female
#Host Body-Site	#Urogenital tract	#Vagina
#Infection	#Patient	-

Isolation

@ref	Sample type
55597	Human vagina,pregnant woman
34393	Human, Pregnant woman, vagina

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
34393	1	Risk group (French classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM3800146v1 assembly for Lactobacillus crispatus LMG11440	contig	GCA_038001465	47770	45.54

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	The role of acidification in the inhibition of Neisseria gonorrhoeae by vaginal lactobacilli during anaerobic growth.	Graver MA, Wade JJ.	Ann Clin Microbiol Antimicrob	10.1186/1476-0711-10-8	2011
	The highly autoaggregative and adhesive phenotype of the vaginal Lactobacillus plantarum strain CMPG5300 is sortase dependent.	Malik S, Petrova MI, Claes IJ, Verhoeven TL, Busschaert P, Vaneechoutte M, Lievens B, Lambrichts I, Siezen RJ, Balzarini J, Vanderleyden J, Lebeer S.	Appl Environ Microbiol	10.1128/aem.00926-13	2013
	Protease activities of vaginal Porphyromonas species disrupt coagulation and extracellular matrix in the cervicovaginal niche.	Lithgow KV, Buchholz VCH, Ku E, Konschuh S, D'Aubeterre A, Sycuro LK.	NPJ Biofilms Microbiomes	10.1038/s41522-022-00270-7	2022

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#34393	Collection of Institut Pasteur ; Curators of the CIP; CIP 103603
#55597	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 42897
#68371	Automatically annotated from API 50CH acid .
#68379	Automatically annotated from API Coryne .
#68380	Automatically annotated from API rID32A .
#68381	Automatically annotated from API rID32STR .
#68382	Automatically annotated from API zym .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Lactobacillus crispatus (CIP 103603, NCTC 4505, NCIMB 4505)

Name and taxonomic classification