Lactobacillus acidophilus (CIP 103596, ATCC 4355, VPI 0328)

Name: Lactobacillus acidophilus (CIP 103596, ATCC 4355, VPI 0328)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 135293

Type strain

Strain Designation R-1-1

Culture col. no. CIP 103596 ATCC 4355 VPI 0328 LMG 11469 KCTC 3140 2 more

NCBI tax ID(s) 1579

Special Collections JCM KCTC CIP

History T. Mitsuoka S1-64 <-- ISL <-- ATCC 4355 <-- W. L. Kulp R-1-1. CIP <- 1992, F. Gasser, Inst. Pasteur, Paris, France: strain VPI 0328, Lactobacillus acidophilus, group A1 <- ATCC <- W.L. Kulp: strain R-1-1

Links

Lactobacillus acidophilus R-1-1 is a facultative anaerobe, mesophilic, Gram-positive prokaryote of the family Lactobacillaceae.

Gram-positive rod-shaped facultative anaerobe mesophilic 16S sequence

Name and taxonomic classification

SI-ID 3198

LMG 11469,ATCC 4355,JCM 1021,CIP 103596,KCTC 3140

@ref 20215

Last LPSN update 2026-02-09 11:32:23

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Lactobacillus

Species Lactobacillus acidophilus

Full scientific name Lactobacillus acidophilus (Moro 1900) Hansen and Mocquot 1970 (Approved Lists 1980)

Synonyms (1)

"Bacillus acidophilus"

BacDive ID	Other strains from **Lactobacillus acidophilus** (35)	Type strain
6404	L. acidophilus L917, Scav, DSM 20079, ATCC 4356, NCDO 1748, ... (type strain)
6403	L. acidophilus A 16, DSM 9126, ATCC 13651, NCDO 1360
6405	L. acidophilus DSM 20242, JCM 2124
135119	L. acidophilus CIP 104455
135306	L. acidophilus 4B, CIP 103597, ATCC 832, NCDO 1, NCTC 1723, ...
135307	L. acidophilus 43, CIP 103598, ATCC 314, JCM 2121
136312	L. acidophilus CIP 104457, ATCC 11975, NCDO 2, NCTC 1899, ...
137096	L. acidophilus CIP 103600, VPI 11473
137247	L. acidophilus A18, CIP 103601
137642	L. acidophilus Pak, CIP 103595, ATCC 4357, NCDO 1697, NCIMB ...
137667	L. acidophilus 65 AC, CIP 104463
137731	L. acidophilus 184.85, CIP 101490
137879	L. acidophilus 174-86, CIP 102104
137884	L. acidophilus 219-86, CIP 102108
138689	L. acidophilus CIP 102079
141878	L. acidophilus CCUG 8032
142532	L. acidophilus CCUG 12853, LMG 8151
145069	L. acidophilus CCUG 27599
145073	L. acidophilus CCUG 27603
145405	L. acidophilus CCUG 28373
145772	L. acidophilus CCUG 29549 B
146144	L. acidophilus CCUG 30451
146219	L. acidophilus CCUG 30632
147334	L. acidophilus CCUG 33386 A
147807	L. acidophilus CCUG 34264
149472	L. acidophilus CCUG 37804
160902	L. acidophilus JCM 1028, KCTC 3145, LMG 18178
160907	L. acidophilus JCM 1033, KCTC 3150
160908	L. acidophilus JCM 1034, KCTC 3151
160915	L. acidophilus JCM 1035, KCTC 3152
161124	L. acidophilus JCM 11047
161209	L. acidophilus JCM 1229, KCTC 3168
165573	L. acidophilus JCM 7711
170959	L. acidophilus 61N, CRBIP24.117
170960	L. acidophilus A31, CRBIP24.142

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility	Confidence
125438	negative			99.5
34370	positive	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Growth	Composition	Medium link
34370	MEDIUM 40- for Lactobacillus and Leuconostoc		Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
34370	CIP Medium 40			Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
34370	positive	growth	37	mesophilic
34370	positive	growth	30-45
34370	negative	growth	5
34370	negative	growth	10
34370	negative	growth	15
67770	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

34370

Oxygen tolerancefacultative anaerobe

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	+	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	+	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	+	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	+	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	+	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
34370	17632 ChEBI	nitrate	-	reduction
34370	17632 ChEBI	nitrate	+	respiration
34370	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	+	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	+	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
34370	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
34370	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
34370	catalase	-	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	-		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
34370	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
34370	ornithine decarboxylase	-	4.1.1.17
34370	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
34370	-	-	+	-	-	+	+	+	-	-	+	+	+	+	-	+	+	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
34370	not determinedn.d.	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	-	+	+	+	+	+	+	+	+	-	+	+	-	-	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
34370	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
34370		USA	USA	North America
67770	Rat intestine
34370	Animal, Rat	United States of America	USA	North America

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
34370	1	Risk group (French classification)

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
124043	Lactobacillus acidophilus gene for 16S rRNA, partial sequence, strain: JCM 1021.	AB289011	681		1579

GC content

@ref	GC-content (mol%)	Method
67770	33	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Klebsiella pneumoniae ATCC 4355
NCBI: GCA_010374775.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	99.50	no
125438	anaerobic	anaerobicⓘ	no	97.68	no
125438	aerobic	aerobicⓘ	no	59.10	yes
125438	spore-forming	spore-formingⓘ	no	89.85	no
125438	thermophilic	thermophileⓘ	no	98.50	yes
125438	flagellated	motile2+ⓘ	no	78.34	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Screening and Characterization of Some Lactobacillaceae for Detection of Cholesterol-Lowering Activities.	Frappier M, Auclair J, Bouasker S, Gunaratnam S, Diarra C, Millette M.	Probiotics Antimicrob Proteins	10.1007/s12602-022-09959-9	2022
Enzymology	The domestication of the probiotic bacterium Lactobacillus acidophilus.	Bull MJ, Jolley KA, Bray JE, Aerts M, Vandamme P, Maiden MC, Marchesi JR, Mahenthiralingam E.	Sci Rep	10.1038/srep07202	2014
	Freeze-Drying Injury of Lactobacillus Acidophilus	Bozoglu TF, Gurakan GC.	J Food Prot		1989
Enzymology	Development of a differential medium for bile salt hydrolase-active Lactobacillus spp.	Dashkevicz MP, Feighner SD.	Appl Environ Microbiol	10.1128/aem.55.1.11-16.1989	1989
	The isolation and cultivation of Lactobacillus bifidus; a comparison of branched and unbranched strains.	NORRIS RF, FLANDERS T, TOMARELLI RM, GYORGY P.	J Bacteriol	10.1128/jb.60.6.681-696.1950	1950
Metabolism	Lactobacillus surface layer proteins: structure, function and applications.	Hynonen U, Palva A.	Appl Microbiol Biotechnol	10.1007/s00253-013-4962-2	2013
Phylogeny	Use of colony-based bacterial strain typing for tracking the fate of Lactobacillus strains during human consumption.	Mahenthiralingam E, Marchbank A, Drevinek P, Garaiova I, Plummer S.	BMC Microbiol	10.1186/1471-2180-9-251	2009
	In vitro prebiotic effects and quantitative analysis of Bulnesia sarmienti extract.	Reza MA, Hossain MA, Lee SJ, Kim JC, Park SC.	J Food Drug Anal	10.1016/j.jfda.2016.03.015	2016
	Deciphering the anti-obesity mechanisms of pharmabiotic probiotics through advanced multiomics analysis.	Kim YR, Choi TR, Jo SH, Song WS, Kim T, Kim MG, Baek JH, Kwon SY, Choi BG, Seo SW, Jang CS, Yang YH, Kim YG.	iScience	10.1016/j.isci.2025.111890	2025
	The Potential Role of Phytonutrients Flavonoids Influencing Gut Microbiota in the Prophylaxis and Treatment of Inflammatory Bowel Disease.	Wang L, Gao M, Kang G, Huang H.	Front Nutr	10.3389/fnut.2021.798038	2021
Metabolism	Lactic acid bacteria convert human fibroblasts to multipotent cells.	Ohta K, Kawano R, Ito N.	PLoS One	10.1371/journal.pone.0051866	2012
	Mature Biofilm Degradation by Potential Probiotics: Aggregatibacter actinomycetemcomitans versus Lactobacillus spp.	Jaffar N, Ishikawa Y, Mizuno K, Okinaga T, Maeda T.	PLoS One	10.1371/journal.pone.0159466	2016
	Freeze-Drying Injury of Lactobacillus Acidophilus.	Bozoglu TF, Gurakan GC	J Food Prot	10.4315/0362-028X-52.4.259	1989
Phylogeny	Isolation and partial amino acid sequence of bacteriocins produced by Lactobacillus acidophilus.	Tahara T, Kanatani K	Biosci Biotechnol Biochem		1997
Phylogeny	Isolation and Partial Amino Add Sequence of Bacteriocins Produced by Lactobacillus acidophilus.	Tahara T, Kanatani K	Biosci Biotechnol Biochem	10.1271/bbb.61.884	1997

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#34370	Collection of Institut Pasteur ; Curators of the CIP; CIP 103596
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Lactobacillus acidophilus (CIP 103596, ATCC 4355, VPI 0328)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Metabolite utilization

Metabolite tests

Enzymes

API zym

API 50CHac

API biotype100

Isolation, sampling and environmental information

Isolation

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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