Name: Clostridioides difficile W 1194
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 135237

Type strain:

Species: Clostridioides difficile

Strain Designation: W 1194

Culture col. no.: CIP 104903, ATCC 43594, CCUG 37779

Strain history: CIP <- 1996, J.P. Carlier, Inst. Pasteur, Paris, France: strain AIP 10131 Clostridium difficile A <- M. Delmée, Leuven, Belgium: strain W 1194

NCBI tax ID(s): 1496 (species)

SI-ID 41929

ATCC 43594, CCUG 37779, CIP 104903

Other strains from Clostridioides difficile

Section

Clostridioides difficile W 1194 is an anaerobe, mesophilic, Gram-positive bacterium of the family Peptostreptococcaceae.

Gram-positive
rod-shaped
anaerobe
mesophilic
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Peptostreptococcaceae*
	Genus	*Clostridioides*
	Species	**Clostridioides difficile**
	Full Scientific Name (LPSN)	Clostridioides difficile (Hall and O'Toole 1935) Lawson et al. 2016

	Synonym	"Bacillus* difficilis"*
	Synonym	"Peptoclostridium* difficile"*
	Synonym	Clostridium difficile

Information on morphological and physiological properties Morphology

[Ref.: #34221]	Gram stain	positive

[Ref.: #34221]	Cell shape	rod-shaped

[Ref.: #34221]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #34221]

Culture medium

MEDIUM 20 - for Anaerobic bacteria

[Ref.: #34221]

Culture medium growth

[Ref.: #34221]

Culture medium composition

expand / minimize

[Ref.: #34221]

Culture medium

CIP Medium 20

[Ref.: #34221]

Culture medium growth

[Ref.: #34221]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #34221]			growth	37 °C
[Ref.: #53865]			growth	37 °C

[Ref.: #34221]	Temperature range	mesophilic
[Ref.: #53865]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #53865]	Oxygen tolerance	anaerobe
[Ref.: #34221]	Oxygen tolerance	anaerobe

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #34221]		nitrate ChEBI	-	reduction
[Ref.: #34221]		nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #34221]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #34221]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #34221]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #34221]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #34221]	8288	-	-	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #53865]	Sample type/isolated from	Human feces,patient with pseudomembranous colitis
[Ref.: #53865]	Geographic location (country and/or sea, region)	Brussels
[Ref.: #53865]	Country	Belgium
[Ref.: #53865]	Country ISO 3 Code	BEL
[Ref.: #53865]	Continent	Europe

[Ref.: #34221]	Sample type/isolated from	Human, Feces
[Ref.: #34221]	Country	Belgium
[Ref.: #34221]	Country ISO 3 Code	BEL
[Ref.: #34221]	Continent	Europe

[Ref.: #34221]	Country	Belgium
[Ref.: #34221]	Country ISO 3 Code	BEL
[Ref.: #34221]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host Body Product	#Gastrointestinal tract	#Feces (Stool)
#Host Body-Site	#Gastrointestinal tract	#Large intestine
#Host	#Human	-
#Infection	#Inflammation	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #34221]

Biosafety level

Risk group (French classification)

Availability in culture collections External links

[Ref.: #34221]

Culture collection no.

CIP 104903, ATCC 43594, CCUG 37779

[Ref.: #92633]

SI-ID 41929

Literature:

Title	Authors	Journal	DOI	Year
Physical map of the Clostridium difficile chromosome.	Norwood DA Jr, Sands JA	Gene	10.1016/s0378-1119(97)00443-5	1997	*
Exosporial membrane plasticity of Clostridium sporogenes and Clostridium difficile.	Panessa-Warren BJ, Tortora GT, Warren JB	Tissue Cell	10.1016/s0040-8166(97)80031-6	1997	*
Macrofragment localization of the toxin A and toxin B genes of Clostridium difficile.	Norwood DA Jr, Sands JA	Clin Diagn Lab Immunol	10.1128/cdli.3.6.782-785.1996	1996	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#34221 Collection of Institut Pasteur ; Curators of the CIP; CIP 104903
#53865 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 37779

#68382

Automatically annotated from API zym .

#92633

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID41929.1 )

* These data were automatically processed and therefore are not curated

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