Actinomycetospora chiangmaiensis (DSM 45062, JCM 15998, NBRC 104400)

Name: Actinomycetospora chiangmaiensis (DSM 45062, JCM 15998, NBRC 104400)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13499

Type strain

Culture col. no. DSM 45062 JCM 15998 NBRC 104400 YIM 0006 CCTCC AA 205017 1 more

NCBI tax ID(s) 1120948 402650

Special Collections DSMZ JCM Wink compendium Literature strains

History <- J. Wiese, IFM Geomar, Kiel <- Y. Jiang DSM 45062 <-- J. Wiese <-- Y. Jiang YIM 0006.

Links

Actinomycetospora chiangmaiensis DSM 45062 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from soil sample from tropical rain forest.

spore-forming Gram-positive aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 389478

TBRC 8064,DSM 45062,JCM 15998,LMG 28635,NBRC 104400

@ref 20215

Last LPSN update 2026-02-09 11:21:09

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Pseudonocardiales

Family Pseudonocardiaceae

Genus Actinomycetospora

Species Actinomycetospora chiangmaiensis

Full scientific name Actinomycetospora chiangmaiensis Jiang et al. 2008

Morphology

Cell morphology

@ref	Gram stain	Motility	Confidence
32109	positive
125439	positive		97.9

Colony morphology

@ref	Colony color	Incubation period	Medium used
20161	Melon yellow (1028)	10-14 days	ISP 2
20161	Melon yellow (1028)	10-14 days	ISP 3
20161	Melon yellow (1028)	10-14 days	ISP 4
20161	Melon yellow (1028)	10-14 days	ISP 5
20161	Melon yellow (1028)	10-14 days	ISP 6
20161	Melon yellow (1028)	10-14 days	ISP 7

Multicellular morphology

@ref	Forms multicellular complex	Medium name
20161		ISP 2
20161		ISP 3
20161		ISP 4
20161		ISP 5
20161		ISP 6
20161		ISP 7

Pigmentation

32109

Productionyes

Multimedia

@ref:	66793
multimedia content:	EM_DSM_45062_1.jpg
multimedia.multimedia content:	EM_DSM_45062_1.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_45062_2.jpg
multimedia.multimedia content:	EM_DSM_45062_2.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_45062_3.jpg
multimedia.multimedia content:	EM_DSM_45062_3.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_45062_4.jpg
multimedia.multimedia content:	EM_DSM_45062_4.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_45062_5.jpg
multimedia.multimedia content:	EM_DSM_45062_5.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
12307	BHI MEDIUM (DSMZ Medium 215)	Medium recipe at MediaDive	Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
12307	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
20161	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20161	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20161	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20161	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20161	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20161	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

Culture temp

@ref	Growth	Type	Temperature (°C)
12307	positive	growth	28
20161	positive	optimum	28
67770	positive	growth	28

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
32109	aerobe
125439	obligate aerobe	98

Spore formation

32109

Spore formationyes

Observation

67770

Observationquinones: MK-9(H₄), MK-8(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
20161	22599 ChEBI	arabinose	-
32109	22653 ChEBI	asparagine	+	carbon source
20161	62968 ChEBI	cellulose	-
20161	28757 ChEBI	fructose	+
32109	28757 ChEBI	fructose	+	carbon source
20161	17234 ChEBI	glucose	+
32109	17234 ChEBI	glucose	+	carbon source
32109	17754 ChEBI	glycerol	+	carbon source
32109	15428 ChEBI	glycine	+	carbon source
32109	27570 ChEBI	histidine	+	carbon source
32109	17716 ChEBI	lactose	+	carbon source
20161	29864 ChEBI	mannitol	+
20161	17268 ChEBI	myo-inositol	+
20161	16634 ChEBI	raffinose	-
20161	26546 ChEBI	rhamnose	-
32109	26546 ChEBI	rhamnose	+	carbon source
32109	33942 ChEBI	ribose	+	carbon source
32109	30911 ChEBI	sorbitol	+	carbon source
20161	17992 ChEBI	sucrose	+
32109	17992 ChEBI	sucrose	+	carbon source
20161	18222 ChEBI	xylose	-
32109	18222 ChEBI	xylose	+	carbon source

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	+		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
20161	not determinedn.d.	-	+	+	+	+	+	-	-	-	+	-	-	-	-	+	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Forest
#Environmental	#Terrestrial	#Soil
#Climate	#Hot	#Tropical

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
12307	soil sample from tropical rain forest	Chiang Mai	Thailand	THA	Asia
67770	Soil from a tropical rainforest	Chiang Mai	Thailand	THA	Asia

Taxonmaps

69479

Global distribution of 16S sequence AM398646 (>99% sequence identity) for Actinomycetospora chiangmaiensis from Microbeatlas

Aquatic Samples	82
Soil Samples	537
Animal Samples	303
Plant Samples	72
Total Samples	994

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
12307	1	Risk group (German classification) According to TRBA 466
20161	1	German classification According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM37962v1 assembly for Actinomycetospora chiangmaiensis DSM 45062	scaffold	GCA_000379625	1120948.3	2515154015	1120948	71.59

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
12307	Actinostreptospora chiangmaiensis partial 16S rRNA gene, type strain YIM 0006T	AM398646	1476		402650

GC content

@ref	GC-content (mol%)	Method
12307	69.03
32109	69
67770	69	high performance liquid chromatography (HPLC)
67770	74	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Actinomycetospora chiangmaiensis DSM 45062
NCBI: GCA_000379625

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	54.10	no
125439	motility	BacteriaNetⓘ	no	70.10	no
125439	gram_stain	BacteriaNetⓘ	positive	97.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.00	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Actinomycetospora chiangmaiensis DSM 45062
PATRIC: 1120948.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	89.05	no
125438	anaerobic	anaerobicⓘ	no	95.54	yes
125438	spore-forming	spore-formingⓘ	yes	76.58	yes
125438	aerobic	aerobicⓘ	yes	87.42	yes
125438	thermophilic	thermophileⓘ	no	93.90	yes
125438	flagellated	motile2+ⓘ	no	86.69	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
	Revealing reaction intermediates in one-carbon elongation by thiamine diphosphate/CoA-dependent enzyme family.	Kim Y, Lee SH, Gade P, Nattermann M, Maltseva N, Endres M, Chen J, Wichmann P, Hu Y, Marchal DG, Yoshikuni Y, Erb TJ, Gonzalez R, Michalska K, Joachimiak A.	Commun Chem	10.1038/s42004-024-01242-y	2024
	Mechanistic details of the actinobacterial lyase-catalyzed degradation reaction of 2-hydroxyisobutyryl-CoA.	Zahn M, Konig G, Pham HVC, Seroka B, Lazny R, Yang G, Ouerfelli O, Lotowski Z, Rohwerder T.	J Biol Chem	10.1016/j.jbc.2021.101522	2022
Metabolism	Discovery of gene cluster for mycosporine-like amino acid biosynthesis from Actinomycetales microorganisms and production of a novel mycosporine-like amino acid by heterologous expression.	Miyamoto KT, Komatsu M, Ikeda H.	Appl Environ Microbiol	10.1128/aem.00727-14	2014
Phylogeny	iSeq 100 for metagenomic pathogen screening in ticks.	Kim JY, Yi MH, Mahdi AAS, Yong TS.	Parasit Vectors	10.1186/s13071-021-04852-w	2021
	Microbial communities in the tropical air ecosystem follow a precise diel cycle.	Gusareva ES, Acerbi E, Lau KJX, Luhung I, Premkrishnan BNV, Kolundzija S, Purbojati RW, Wong A, Houghton JNI, Miller D, Gaultier NE, Heinle CE, Clare ME, Vettath VK, Kee C, Lim SBY, Chenard C, Phung WJ, Kushwaha KK, Nee AP, Putra A, Panicker D, Yanqing K, Hwee YZ, Lohar SR, Kuwata M, Kim HL, Yang L, Uchida A, Drautz-Moses DI, Junqueira ACM, Schuster SC.	Proc Natl Acad Sci U S A	10.1073/pnas.1908493116	2019
Genetics	Genome-Based Taxonomic Classification of the Phylum Actinobacteria.	Nouioui I, Carro L, Garcia-Lopez M, Meier-Kolthoff JP, Woyke T, Kyrpides NC, Pukall R, Klenk HP, Goodfellow M, Goker M.	Front Microbiol	10.3389/fmicb.2018.02007	2018
Metabolism	Actinobacterial Degradation of 2-Hydroxyisobutyric Acid Proceeds via Acetone and Formyl-CoA by Employing a Thiamine-Dependent Lyase Reaction.	Rohwerder T, Rohde MT, Jehmlich N, Purswani J	Front Microbiol	10.3389/fmicb.2020.00691	2020
Phylogeny	Actinomycetospora aeridis sp. nov., Actinomycetospora flava sp. nov. and Actinomycetospora aurantiaca sp. nov., endophytic actinobacteria isolated from wild orchid (Aerides multiflora Roxb).	Suriyachadkun C, Ngaemthao W, Pujchakarn T, Sakdapetsiri C, Ayawong C, Chairote K, Chunhametha S.	Int J Syst Evol Microbiol	10.1099/ijsem.0.006505	2024
Phylogeny	Description of Actinomycetospora chibensis sp. nov., Actinomycetospora chlora sp. nov., Actinomycetospora cinnamomea sp. nov., Actinomycetospora corticicola sp. nov., Actinomycetospora lutea sp. nov., Actinomycetospora straminea sp. nov. and Actinomycetospora succinea sp. nov. and emended description of the genus Actinomycetospora.	Tamura T, Ishida Y, Hamada M, Otoguro M, Yamamura H, Hayakawa M, Suzuki KI.	Int J Syst Evol Microbiol	10.1099/ijs.0.024166-0	2011
Phylogeny	Actinomycetospora endophytica sp. nov., isolated from wild orchid (Podochilus microphyllus Lindl.) in Thailand.	Sakdapetsiri C, Ngaemthao W, Suriyachadkun C, Duangmal K, Kitpreechavanich V	Int J Syst Evol Microbiol	10.1099/ijsem.0.002938	2018
Phylogeny	Actinomycetospora atypica sp. nov., a novel soil actinomycete and emended description of the genus Actinomycetospora.	Zhang Y, Liu C, Zhang J, Shen Y, Li C, He H, Wang X, Xiang W	Antonie Van Leeuwenhoek	10.1007/s10482-014-0143-2	2014
Phylogeny	Actinomycetospora iriomotensis sp. nov., a novel actinomycete isolated from a lichen sample.	Yamamura H, Ashizawa H, Nakagawa Y, Hamada M, Ishida Y, Otoguro M, Tamura T, Hayakawa M	J Antibiot (Tokyo)	10.1038/ja.2011.15	2011
Phylogeny	Actinomycetospora rishiriensis sp. nov., isolated from a lichen.	Yamamura H, Ashizawa H, Nakagawa Y, Hamada M, Ishida Y, Otoguro M, Tamura T, Hayakawa M	Int J Syst Evol Microbiol	10.1099/ijs.0.028753-0	2010
Phylogeny	Actinomycetospora chiangmaiensis gen. nov., sp. nov., a new member of the family Pseudonocardiaceae.	Jiang Y, Wiese J, Tang SK, Xu LH, Imhoff JF, Jiang CL	Int J Syst Evol Microbiol	10.1099/ijs.0.64976-0	2008
Phylogeny	Actinomycetospora soli sp. nov., isolated from the rhizosphere soil of Averrhoa carambola L.	Chantavorakit T, Duangmal K	Int J Syst Evol Microbiol	10.1099/ijsem.0.005277	2022

References

#12307	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45062
#20161	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#28354	IJSEM 408 2008 ( DOI 10.1099/ijs.0.64976-0 , PubMed 18218940 )
#32109	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28354
#66793	Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Actinomycetospora chiangmaiensis (DSM 45062, JCM 15998, NBRC 104400)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Pigmentation

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Observation

Metabolite utilization

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000379625

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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