Saccharopolyspora phatthalungensis (DSM 45584, BCC 35844, NRRL B-24798)

Name: Saccharopolyspora phatthalungensis (DSM 45584, BCC 35844, NRRL B-24798)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13474

Type strain

Strain Designation SR8.15

Culture col. no. DSM 45584 BCC 35844 NRRL B-24798 TISTR 1921 SR8.15 2 more

NCBI tax ID(s) 664693

Special Collections DSMZ JCM Wink compendium Literature strains

History <- D. P. Labeda, NRRL; NRRL B-24798 TISTR 1921 <-- K. Duangmal SR8.15.

Links

Saccharopolyspora phatthalungensis SR8.15 is an aerobe, spore-forming, Gram-positive bacterium that builds an aerial mycelium and was isolated from rhizospheric soil of a rubber tree .

spore-forming Gram-positive aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 400947

JCM 16708,DSM 45584

@ref 20215

Last LPSN update 2026-02-09 11:21:09

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Pseudonocardiales

Family Pseudonocardiaceae

Genus Saccharopolyspora

Species Saccharopolyspora phatthalungensis

Full scientific name Saccharopolyspora phatthalungensis Duangmal et al. 2010

Morphology

Cell morphology

@ref	Gram stain	Confidence
29665	positive
125439	positive	99.9
125439		96.4

Colony morphology

@ref	Colony color	Incubation period	Medium used
21402	Maize yellow	10-14 days	ISP 2
21402		10-14 days	ISP 3
21402		10-14 days	ISP 4
21402		10-14 days	ISP 5
21402	Nut brown (8011)	10-14 days	ISP 7
21402	Ochre brown	10-14 days	Suter with tyrosine
21402	Beige (1001)	10-14 days	Suter without tyrosine

Multicellular morphology

@ref	Complex name	Complex color	Medium name
21402			ISP 2
21402	Aerial Mycelium	Pure white	ISP 3
21402			ISP 4
21402			ISP 5
21402	Aerial Mycelium	Signal white (9003)	ISP 7
21402	Aerial Mycelium	Traffic white	Suter with tyrosine
21402			Suter without tyrosine

Pigmentation

29665

Productionyes

Multimedia

@ref:	17714
multimedia content:	DSM_45584.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_45584.jpg
caption:	Medium 554 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
17714	N-Z-AMINE-MEDIUM (DSMZ Medium 554)	Medium recipe at MediaDive	Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water
17714	GPHF-MEDIUM (DSMZ Medium 553)	Medium recipe at MediaDive	Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
21402	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
21402	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
21402	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
21402	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
21402	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
21402	Suter with tyrosine		Name: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes
21402	Suter without tyrosine		Name: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes

Culture temp

@ref	Growth	Type	Temperature (°C)
17714	positive	growth	28
29665	positive	growth	18-42
29665	positive	optimum	31
67770	positive	growth	28

Culture pH

@ref	Ability	Type	PH	PH range
29665	positive	growth	05-09	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
29665	aerobe
125438	aerobe	93.291
125439	obligate aerobe	99.8

Spore formation

@ref	Spore formation	Confidence
29665
125439		97.4

Halophily

@ref	Salt	Growth	Tested relation	Concentration
21402	NaCl	positive	maximum	7.5 %
29665	NaCl	positive	growth	0-7 %
29665	NaCl	positive	optimum	2.5 %

Observation

67770

Observationquinones: MK-9(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
21402	22599 ChEBI	arabinose	+
21402	62968 ChEBI	cellulose	+
68379	17634 ChEBI	D-glucose	-	fermentation	from API Coryne
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
29665	4853 ChEBI	esculin	+	hydrolysis
68379	4853 ChEBI	esculin	+	hydrolysis	from API Coryne
21402	28757 ChEBI	fructose	+
29665	28757 ChEBI	fructose	+	carbon source
29665	28260 ChEBI	galactose	+	carbon source
68379	5291 ChEBI	gelatin	+	hydrolysis	from API Coryne
21402	17234 ChEBI	glucose	+
29665	17234 ChEBI	glucose	+	carbon source
29665	17754 ChEBI	glycerol	+	carbon source
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
29665	29864 ChEBI	mannitol	+	carbon source
21402	37684 ChEBI	mannose	+
29665	37684 ChEBI	mannose	+	carbon source
21402	17268 ChEBI	myo-inositol	+
29665	17268 ChEBI	myo-inositol	+	carbon source
68379	17632 ChEBI	nitrate	-	reduction	from API Coryne
21402	16634 ChEBI	raffinose	-
21402	26546 ChEBI	rhamnose	-
29665	15963 ChEBI	ribitol	+	carbon source
29665	33942 ChEBI	ribose	+	carbon source
21402	17992 ChEBI	sucrose	+
29665	27082 ChEBI	trehalose	+	carbon source
21402	18222 ChEBI	xylose	-
29665	18222 ChEBI	xylose	+	carbon source

Metabolite production

@ref	Chebi-ID	Metabolite	Production
29665	16136 ChEBI	hydrogen sulfide

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
29665	alkaline phosphatase	+	3.1.3.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68379	alkaline phosphatase	-	3.1.3.1	from API Coryne
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68379	alpha-glucosidase	-	3.2.1.20	from API Coryne
68382	alpha-mannosidase	+	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68379	beta-galactosidase	-	3.2.1.23	from API Coryne
68382	beta-glucosidase	-	3.2.1.21	from API zym
68379	beta-glucosidase	+	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
29665	catalase	+	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
29665	gelatinase	+
68379	gelatinase	+		from API Coryne
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
68379	pyrrolidonyl arylamidase	-	3.4.19.3	from API Coryne
29665	urease	+	3.5.1.5
68382	valine arylamidase	-		from API zym

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
21402	-	not determinedn.d.	-	-	-	-	-	-	+	+/-	+	not determinedn.d.	-	-	-	-	-	-	not determinedn.d.	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
21402	not determinedn.d.	+	+	+	+/-	+/-	-	+	+/-	+/-	+	+/-	-	-	-	-	-	+	+	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Tree
#Host Body-Site	#Plant	#Rhizosphere

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent
17714	rhizospheric soil of a rubber tree (Hevea brasiliensis)		Phatthalung Province	Thailand	THA	Asia
67770	Rhizospheric soil of a rubber tree (Hevea brasiliensis)	Hevea brasiliensis	Phatthalung Province	Thailand	THA	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
17714	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1420339v1 assembly for Saccharopolyspora phatthalungensis DSM 45584	contig	GCA_014203395	664693.10	2863067949	664693	75.5
67770	ASM71591v1 assembly for Saccharopolyspora phatthalungensis NRRL B-24798	scaffold	GCA_000715915	664693.3	2775506925	664693

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
17714	Saccharopolyspora phatthalungensis strain SR8.15 16S ribosomal RNA gene, partial sequence	GQ381309	1448		664693

GC content

@ref	GC-content (mol%)	Method
17714	70.3
67770	70.3	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Saccharopolyspora phatthalungensis NRRL B-24798
NCBI: GCA_000715915

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	97.40	no
125439	motility	BacteriaNetⓘ	no	96.40	no
125439	gram_stain	BacteriaNetⓘ	positive	99.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.80	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Saccharopolyspora phatthalungensis strain DSM 45584
PATRIC: 664693.10

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	87.90	yes
125438	anaerobic	anaerobicⓘ	no	95.94	yes
125438	spore-forming	spore-formingⓘ	yes	85.05	yes
125438	aerobic	aerobicⓘ	yes	93.29	no
125438	thermophilic	thermophileⓘ	no	96.00	no
125438	flagellated	motile2+ⓘ	no	87.61	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
	Gromomycins: An Unprecedented Class of Triterpene Antibiotics Produced by a Novel Biosynthetic Pathway.	Tistechok S, Bratiichuk D, Sucipto H, Gummerlich N, Stierhof M, Gromyko O, Fries F, Fedorenko V, Muller R, Zapp J, Myronovskyi M, Luzhetskyy A.	Angew Chem Int Ed Engl	10.1002/anie.202422270	2025
Genetics	Mining Translation Inhibitors by a Unique Peptidyl-Aminonucleoside Synthetase Reveals Cystocin Biosynthesis and Self-Resistance.	Alferova VA, Zotova PA, Baranova AA, Guglya EB, Belozerova OA, Pipiya SO, Kudzhaev AM, Logunov SE, Prokopenko YA, Marenkova EA, Marina VI, Marina VI, Novikova EA, Komarova ES, Starodumova IP, Bueva OV, Evtushenko LI, Ariskina EV, Kovalchuk SI, Mineev KS, Babenko VV, Sergiev PV, Lukianov DA, Terekhov SS.	Int J Mol Sci	10.3390/ijms252312901	2024
Phylogeny	Saccharopolyspora hattusasensis sp. nov., isolated from soil.	Veyisoglu A, Saygin H, Tatar D, Bektas KI, Sahin N	Antonie Van Leeuwenhoek	10.1007/s10482-017-0921-8	2017
Phylogeny	Saccharopolyspora phatthalungensis sp. nov., isolated from rhizosphere soil of Hevea brasiliensis.	Duangmal K, Mingma R, Thamchaipenet A, Matsumoto A, Takahashi Y	Int J Syst Evol Microbiol	10.1099/ijs.0.018275-0	2009

References

#17714	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45584
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#21402	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#26052	IJSEM 1904 2010 ( DOI 10.1099/ijs.0.018275-0 , PubMed 19783611 )
#29665	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26052
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Saccharopolyspora phatthalungensis (DSM 45584, BCC 35844, NRRL B-24798)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Pigmentation

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Enzymes

API coryne

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_014203395

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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