Saccharopolyspora phatthalungensis SR8.15 | Type strain | DSM 45584, BCC 35844, NRRL B-24798, TISTR 1921, JCM 16708, NBRC 106097

Caption:

Medium 554 28°C

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

Culture medium

N-Z-AMINE-MEDIUM (DSMZ Medium 554)

Culture medium growth

Medium recipe at MediaDive

Culture medium link

Culture medium composition

Culture medium

GPHF-MEDIUM (DSMZ Medium 553)

Culture medium growth

Medium recipe at MediaDive

Culture medium link

Culture medium composition

Culture medium

ISP 2

Culture medium growth

Culture medium composition

Culture medium

ISP 3

Culture medium growth

Culture medium composition

Culture medium

ISP 4

Culture medium growth

Culture medium composition

Culture medium

ISP 5

Culture medium growth

Culture medium composition

Culture medium

ISP 7

Culture medium growth

Culture medium composition

Culture medium

Suter with tyrosine

Culture medium growth

Culture medium composition

Culture medium

Suter without tyrosine

Culture medium growth

Culture medium composition

What are isolation sources categories?

		Temperature
[Ref.: #17714]	growth	28 °C
[Ref.: #29665]	growth	18-42 °C
[Ref.: #29665]	optimum	31 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #17714]	Temperature range	mesophilic
[Ref.: #29665]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #29665]			growth	05-09

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29665]

Oxygen tolerance

aerobe

[Ref.: #29665]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %97.594

	Salt		Salt conc.
[Ref.: #21402]	NaCl	maximum	7.5	%
[Ref.: #29665]	NaCl	growth	0-7	%
[Ref.: #29665]	NaCl	optimum	2.5	%

[Ref.: #67770]

Observation

quinones: MK-9(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #21402]	arabinose ChEBI	+
[Ref.: #21402]	cellulose ChEBI	+
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #29665]	esculin ChEBI	+	hydrolysis
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #21402]	fructose ChEBI	+
[Ref.: #29665]	fructose ChEBI	+	carbon source
[Ref.: #29665]	galactose ChEBI	+	carbon source
[Ref.: #68379]	gelatin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #21402]	glucose ChEBI	+
[Ref.: #29665]	glucose ChEBI	+	carbon source
[Ref.: #29665]	glycerol ChEBI	+	carbon source
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #29665]	mannitol ChEBI	+	carbon source
[Ref.: #21402]	mannose ChEBI	+
[Ref.: #29665]	mannose ChEBI	+	carbon source
[Ref.: #21402]	myo-inositol ChEBI	+
[Ref.: #29665]	myo-inositol ChEBI	+	carbon source
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #21402]	raffinose ChEBI	-
[Ref.: #21402]	rhamnose ChEBI	-
[Ref.: #29665]	ribitol ChEBI	+	carbon source
[Ref.: #29665]	ribose ChEBI	+	carbon source
[Ref.: #21402]	sucrose ChEBI	+
[Ref.: #29665]	trehalose ChEBI	+	carbon source
[Ref.: #21402]	xylose ChEBI	-
[Ref.: #29665]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #29665]		hydrogen sulfide ChEBI	yes

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #29665]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68379]	alkaline phosphatase	-	3.1.3.1	* from API Coryne
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	-	3.2.1.20	* from API Coryne, API zym
[Ref.: #68382]	alpha-mannosidase	+	3.2.1.24	* from API zym
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne, API zym
[Ref.: #68379]	beta-glucosidase	+	3.2.1.21	* from API Coryne
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #29665]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #29665]	gelatinase	+
[Ref.: #68379]	gelatinase	+		* from API Coryne
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #29665]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #21402]	528	-	not determinedn.d.	-	-	-	-	-	-	+	+/-	+	not determinedn.d.	-	-	-	-	-	-	not determinedn.d.	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #21402]	1043	not determinedn.d.	+	+	+	+/-	+/-	-	+	+/-	+/-	+	+/-	-	-	-	-	-	+	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #17714]	Sample type/isolated from	rhizospheric soil of a rubber tree (Hevea brasiliensis)
[Ref.: #17714]	Geographic location (country and/or sea, region)	Phatthalung Province
[Ref.: #17714]	Country	Thailand
[Ref.: #17714]	Country ISO 3 Code	THA
[Ref.: #17714]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Rhizospheric soil of a rubber tree (Hevea brasiliensis)
[Ref.: #67770]	Host species	Hevea brasiliensis
[Ref.: #67770]	Geographic location (country and/or sea, region)	Phatthalung Province
[Ref.: #67770]	Country	Thailand
[Ref.: #67770]	Country ISO 3 Code	THA
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Tree
#Host Body-Site	#Plant	#Rhizosphere

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #17714]	Saccharopolyspora phatthalungensis strain SR8.15 16S ribosomal RNA gene, partial sequence	GQ381309	1448	ENA	664693 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Saccharopolyspora phatthalungensis NRRL B-24798	GCA_000715915	scaffold	GenBank	664693 tax ID
[Ref.: #66792]	Saccharopolyspora phatthalungensis DSM 45584	GCA_014203395	contig	GenBank	664693 tax ID	*
[Ref.: #66792]	Saccharopolyspora phatthalungensis strain DSM 45584	664693.10	wgs	PATRIC	664693 tax ID	*
[Ref.: #66792]	Saccharopolyspora phatthalungensis DSM 45584	2863067949	draft	JGI IMG/M	664693 tax ID	*

[Ref.: #17714]	GC-content	70.3 mol%
[Ref.: #67770]	GC-content	70.3 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Saccharopolyspora phatthalungensis NRRL B-24798 NCBI: GCA_000715915.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Saccharopolyspora phatthalungensis strain DSM 45584 PATRIC: 664693.10
[Ref.: #69480]	gram-positive	yes	91.166	yes
[Ref.: #69480]	anaerobic	no	98.817	yes
[Ref.: #69480]	halophile	no	72.736	no
[Ref.: #69480]	spore-forming	yes	93.836	yes
[Ref.: #69480]	glucose-util	yes	87.462	yes
[Ref.: #69480]	thermophile	no	93.203	yes
[Ref.: #69480]	flagellated	no	98.262	yes
[Ref.: #69480]	motile	no	94.3	yes
[Ref.: #69480]	aerobic	yes	92.263	no
[Ref.: #69480]	glucose-ferment	no	92.179	yes

Availability in culture collections External links