Name: Halobacillus opalescens CIP 108072
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 134682

Type strain:

Species: Halobacillus opalescens

Culture col. no.: CIP 108072, YIM KN2

Strain history: CIP <- 2003, W.J. Li, Yunnan Univ., Kunming, China: strain YIM KN2

Section

Halobacillus opalescens CIP 108072 is a spore-forming, mesophilic, Gram-positive prokaryote of the family Not assigned to family.

Gram-positive
motile
rod-shaped
spore-forming
mesophilic

Information on the name and the taxonomic classification. Name and taxonomic classification

[Ref.: #33197]	Phylum	Not assigned to order
[Ref.: #33197]	Class	Not assigned to order
[Ref.: #33197]	Order	Not assigned to order
[Ref.: #33197]	Family	Not assigned to family
[Ref.: #33197]	Genus	Halobacillus
[Ref.: #33197]	Species	Halobacillus opalescens
[Ref.: #33197]	Type strain

Information on morphological and physiological properties Morphology

[Ref.: #33197]	Gram stain	positive

[Ref.: #33197]	Cell shape	rod-shaped

[Ref.: #33197]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #33197]

Culture medium

Marine agar (MA)

[Ref.: #33197]

Culture medium growth

[Ref.: #33197]

Culture medium composition

expand / minimize

[Ref.: #33197]

Culture medium

CIP Medium 13

[Ref.: #33197]

Culture medium growth

[Ref.: #33197]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #33197]		growth	25 °C
[Ref.: #33197]		growth	15-37 °C
[Ref.: #33197]	no	growth	5 °C
[Ref.: #33197]	no	growth	41 °C

[Ref.: #33197]	Temperature range	mesophilic
[Ref.: #33197]	Temperature range	psychrophilic
[Ref.: #33197]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #33197]

Ability of spore formation

yes

	Salt	Tested relation		Salt conc.
[Ref.: #33197]	NaCl	no	growth	0	%
[Ref.: #33197]	NaCl	no	growth	2	%
[Ref.: #33197]	NaCl	no	growth	4	%
[Ref.: #33197]	NaCl	no	growth	6	%
[Ref.: #33197]	NaCl	no	growth	8	%
[Ref.: #33197]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #33197]	citrate ChEBI	-	carbon source
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68368]	D-glucose ChEBI	-	fermentation	* from API 20E
[Ref.: #33197]	esculin ChEBI	-	hydrolysis
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #33197]	glucose ChEBI	-	fermentation
[Ref.: #33197]	lactose ChEBI	-	fermentation
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #33197]	nitrate ChEBI	-	reduction
[Ref.: #68368]	nitrate ChEBI	-	reduction	* from API 20E
[Ref.: #33197]	nitrite ChEBI	-	reduction
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #33197]	sodium thiosulfate ChEBI	-	builds gas from
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #33197]	indole ChEBI	no
[Ref.: #68368]	acetoin ChEBI	no	* from API 20E
[Ref.: #68368]	dinitrogen ChEBI	no	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E
[Ref.: #68368]	nitrite ChEBI	no	* from API 20E

	Metabolite	Voges-Proskauer-test	Methylred-test	Indole test
[Ref.: #33197]	acetoin ChEBI	-
[Ref.: #33197]	glucose ChEBI		-
[Ref.: #68368]	acetoin ChEBI	-			* from API 20E
[Ref.: #68368]	indole ChEBI			-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #33197]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #33197]	amylase	+
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #33197]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68368]	beta-galactosidase	-	3.2.1.23	* from API 20E
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #33197]	caseinase	+	3.4.21.50
[Ref.: #33197]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #33197]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #33197]	gelatinase	+
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #33197]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #33197]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #33197]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #33197]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #33197]	oxidase	+
[Ref.: #33197]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #33197]	protease	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #33197]	tryptophan deaminase	-
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #33197]	tween esterase	-
[Ref.: #33197]	urease	-	3.5.1.5
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #33197]	15655	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #33197]	10862	-	+	+	+	-	-	-	-	-	-	+	+	-	+	-	-	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #33197]	Sample type/isolated from	Environment, Hypersaline soil
[Ref.: #33197]	Geographic location (country and/or sea, region)	Qinghai
[Ref.: #33197]	Country	China
[Ref.: #33197]	Country ISO 3 Code	CHN
[Ref.: #33197]	Continent	Asia
[Ref.: #33197]	Isolation date	2002

[Ref.: #33197]	Geographic location (country and/or sea, region)	Qinghai Province
[Ref.: #33197]	Country	China
[Ref.: #33197]	Country ISO 3 Code	CHN
[Ref.: #33197]	Continent	Asia
* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #33197]

Biosafety level

Risk group (French classification)

Availability in culture collections External links

[Ref.: #33197]

Culture collection no.

CIP 108072, YIM KN2

References
#33197 Collection of Institut Pasteur ; Curators of the CIP; CIP 108072

#68368

Automatically annotated from API 20E .

#68382

Automatically annotated from API zym .

* These data were automatically processed and therefore are not curated

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