Name: Saccharopolyspora hirsuta DSM 43463
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13447

Type strain:

Species: Saccharopolyspora hirsuta

Culture col. no.: DSM 43463, AS 4.1704, ATCC 27875, CBS 420.74, DSM 43402, IFO 13919, IMET 9709, IMRU 1558, JCM 3170, KCC A-0170, NBRC 13919, NCIB 11079, NRRL B-5792, CECT 3304, CGMCC 4.1317, CGMCC 4.1493, CGMCC 4.1704, IMSNU 20123, KACC 20037, KCTC 9481, NCIMB 11079, NRRL B-16205, RIA 1561, VKM Ac-666, VKM Ac-693

Strain history: KCC A-0170 <-- J. Lacey A1143.

NCBI tax ID(s): 1837 (species)

Other strains from Saccharopolyspora hirsuta

S. hirsuta DSM 43401, IMRU 1461
S. hirsuta A 153, Lacey A 153, DSM 46073, ...
S. hirsuta A 1145, Lacey A 1145, DSM 46074, ...
S. hirsuta A 1132, Lacey A 1132, DSM 46203, ...

Section

Saccharopolyspora hirsuta DSM 43463 is a spore-forming, mesophilic bacterium that was isolated from sugar cane bagasse.

spore-forming
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Pseudonocardiales*
	Family	*Pseudonocardiaceae*
	Genus	*Saccharopolyspora*
	Species	**Saccharopolyspora hirsuta**
	Full Scientific Name (PNU)	Saccharopolyspora hirsuta Lacey and Goodfellow 1975 (Approved Lists 1980)

Information on morphological and physiological properties Morphology

[Ref.: #19573]	Colony color	Beige
[Ref.: #19573]	Incubation period	10-14 days
[Ref.: #19573]	Cultivation medium used	ISP 2

[Ref.: #19573]	Incubation period	10-14 days
[Ref.: #19573]	Cultivation medium used	ISP 3

[Ref.: #19573]	Incubation period	10-14 days
[Ref.: #19573]	Cultivation medium used	ISP 4

[Ref.: #19573]	Incubation period	10-14 days
[Ref.: #19573]	Cultivation medium used	ISP 5

[Ref.: #19573]	Incubation period	10-14 days
[Ref.: #19573]	Cultivation medium used	ISP 6

[Ref.: #19573]	Incubation period	10-14 days
[Ref.: #19573]	Cultivation medium used	ISP 7

[Ref.: #19573]	Multicellular complex forming ability	no
[Ref.: #19573]	Medium Name (multicellularity)	ISP 2

[Ref.: #19573]	Multicellular complex forming ability	no
[Ref.: #19573]	Medium Name (multicellularity)	ISP 3

[Ref.: #19573]	Multicellular complex forming ability	no
[Ref.: #19573]	Medium Name (multicellularity)	ISP 4

[Ref.: #19573]	Multicellular complex forming ability	no
[Ref.: #19573]	Medium Name (multicellularity)	ISP 5

[Ref.: #19573]	Multicellular complex forming ability	no
[Ref.: #19573]	Medium Name (multicellularity)	ISP 6

[Ref.: #19573]	Multicellular complex forming ability	no
[Ref.: #19573]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

[Ref.: #11038]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #11038]

Culture medium growth

[Ref.: #11038]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #11038]

Culture medium composition

expand / minimize

[Ref.: #19573]

Culture medium

ISP 2

[Ref.: #19573]

Culture medium growth

[Ref.: #19573]

Culture medium composition

expand / minimize

[Ref.: #19573]

Culture medium

ISP 3

[Ref.: #19573]

Culture medium growth

[Ref.: #19573]

Culture medium composition

expand / minimize

[Ref.: #19573]

Culture medium

ISP 4

[Ref.: #19573]

Culture medium growth

[Ref.: #19573]

Culture medium composition

expand / minimize

[Ref.: #19573]

Culture medium

ISP 5

[Ref.: #19573]

Culture medium growth

[Ref.: #19573]

Culture medium composition

expand / minimize

[Ref.: #19573]

Culture medium

ISP 6

[Ref.: #19573]

Culture medium growth

[Ref.: #19573]

Culture medium composition

expand / minimize

[Ref.: #19573]

Culture medium

ISP 7

[Ref.: #19573]

Culture medium growth

[Ref.: #19573]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #11038]	growth	28 °C
[Ref.: #19573]	optimum	30 °C
[Ref.: #67770]	growth	40 °C

[Ref.: #11038]	Temperature range	mesophilic
[Ref.: #19573]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #19573]	Spore description	Formation of spores, spore surface smooth
[Ref.: #19573]	Type of spore	spore
[Ref.: #19573]	Ability of spore formation	yes

[Ref.: #11038]	Name of produced compound	KA-5685 (aminoglycoside antibiotic)
[Ref.: #20216]	Name of produced compound	Antibiotic KA-5685 (aminoglycoside)
[Ref.: #19573]	Name of produced compound	Destomycin
[Ref.: #19573]	Name of produced compound	Nargenicin
[Ref.: #19573]	Name of produced compound	Saccharocin
[Ref.: #19573]	Name of produced compound	Sporaricin
[Ref.: #19573]	Name of produced compound	Vanoxonin

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #19573]		NaCl		maximum	2.5	%

[Ref.: #67770]

Observation

quinones: MK-9(H₄), MK-9(H₂)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	+	assimilation	* from API 20NE
[Ref.: #19573]	arabinose ChEBI	-
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE, API 20E
[Ref.: #19573]	cellulose ChEBI	-
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #19573]	fructose ChEBI	+
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE, API 20E
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #19573]	glucose ChEBI	+
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	+	assimilation	* from API 20NE
[Ref.: #19573]	mannitol ChEBI	-
[Ref.: #19573]	myo-inositol ChEBI	+
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #19573]	raffinose ChEBI	-
[Ref.: #19573]	rhamnose ChEBI	-
[Ref.: #19573]	sucrose ChEBI	+
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE, API 20E
[Ref.: #68369]	urea ChEBI	+	hydrolysis	* from API 20NE, API 20E
[Ref.: #19573]	xylose ChEBI	-
	Only first 10 entries are displayed. Click here to see all.

	Metabolite
[Ref.: #68368]	acetoin ChEBI	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	* from API 20E
[Ref.: #68369]	indole ChEBI	* from API 20NE, API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68369]		indole ChEBI		-	* from API 20NE, API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE, API 20E
[Ref.: #68368]	beta-galactosidase	-	3.2.1.23	* from API 20E
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym, API 20NE
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68369]	gelatinase	+		* from API 20NE, API 20E
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68369]	urease	+	3.5.1.5	* from API 20NE, API 20E
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #19573]	424	-	-	-	-	+	-	+	-	-	+	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #11038]	22687	-	-	-	-	+	+	+	+	+	+	+	+	+	+	+	-	+	+	+	+/-	not determinedn.d.
[Ref.: #11038]	22688	-	-	-	-	+	+	+	+	+	+/-	+	+	+	+	+	-	+	+	+	+/-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #19573]	410	not determinedn.d.	+	-	+	-	-	+	+	+	+	+	+	-	+	-	+	+	+	+	-
[Ref.: #11038]	6486	-	+	+	+	-	+	-	-	-	-	+	-	-	+/-	-	+	+	+	-	-
[Ref.: #11038]	6485	-	+	+	+	-	+	-	-	-	-	+	+	-	-	-	+	+	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #11038]	Sample type/isolated from	sugar cane bagasse

[Ref.: #67770]	Sample type/isolated from	Sugar-cane bagasse
[Ref.: #67770]	Geographic location (country and/or sea, region)	Trinidad, West Indies
[Ref.: #67770]	Country	Trinidad and Tobago
[Ref.: #67770]	Country ISO 3 Code	TTO
[Ref.: #67770]	Continent	North America
* marker position based on {}

Isolation sources categories

#Host

#Plants

#Herbaceous plants (Grass,Crops)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence DQ381814 (>99% sequence identity) for Saccharopolyspora from Microbeatlas

Aquatic Samples	198
Soil Samples	1276
Animal Samples	987
Plant Samples	377
Total Samples	2838

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11038]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #19573]	Biosafety level	1	Hazard group

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Saccharopolyspora hirsuta strain ATCC 27875 16S ribosomal RNA gene, complete sequence	DQ381814	1422	ENA	1837 tax ID	*
[Ref.: #20218]	S.hirsuta 16S ribosomal RNA	M20388	1284	ENA	1837 tax ID	*
[Ref.: #20218]	Saccharopolyspora hirsuta strain ATCC 27875 16S ribosomal RNA gene, partial sequence	U93341	1487	ENA	1837 tax ID	*
[Ref.: #20218]	Saccharopolyspora hirsuta 16S rRNA (part.)	X53196	1381	ENA	1837 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Saccharopolyspora hirsuta VKM Ac-666	GCA_008630535	contig	GenBank	1837 tax ID

[Ref.: #67770]

GC-content

76.9 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #11038]

Culture collection no.

DSM 43463, AS 4.1704, ATCC 27875, CBS 420.74, DSM 43402, IFO 13919, IMET 9709, IMRU 1558, JCM 3170, KCC A-0170, NBRC 13919, NCIB 11079, NRRL B-5792, CECT 3304, CGMCC 4.1317, CGMCC 4.1493, CGMCC 4.1704, IMSNU 20123, KACC 20037, KCTC 9481, NCIMB 11079, NRRL B-16205, RIA 1561, VKM Ac-666, VKM Ac-693

[Ref.: #20218]

Associated Passport(s) in StrainInfo

168339, 236678, 106078, 229440, 168342, 168343, 168344, 168345, 168346, 106081, 333515, 106082, 106083

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Saccharopolyspora soli sp. nov., isolated from Northern Cyprus soil.	Veyisoglu A, Tatar D, Saygin H, Cetin D, Guven K, Sahin N	Arch Microbiol	10.1007/s00203-022-02994-8	2022	*
Metabolism	Steroid Metabolism in Thermophilic Actinobacterium Saccharopolyspora hirsuta VKM Ac-666(T).	Lobastova T, Fokina V, Tarlachkov S, Shutov A, Bragin E, Kazantsev A, Donova M	Microorganisms	10.3390/microorganisms9122554	2021	*
Genetics	Draft Genome Sequence of the Moderately Thermophilic Actinobacterial Steroid-Transforming Saccharopolyspora hirsuta subsp. hirsuta Strain VKM Ac-666(T).	Lobastova TG, Fokina VV, Bragin EY, Shtratnikova VY, Starodumova IP, Tarlachkov SV, Donova MV	Microbiol Resour Announc	10.1128/MRA.01327-19	2020	*
Metabolism	Microbiological synthesis of stereoisomeric 7(alpha/beta)-hydroxytestololactones and 7(alpha/beta)-hydroxytestolactones.	Lobastova TG, Khomutov SM, Shutov AA, Donova MV	Appl Microbiol Biotechnol	10.1007/s00253-019-09828-6	2019	*
Phylogeny	Saccharopolyspora antimicrobica sp. nov., an actinomycete from soil.	Yuan LJ, Zhang YQ, Guan Y, Wei YZ, Li QP, Yu LY, Li WJ, Zhang YQ	Int J Syst Evol Microbiol	10.1099/ijs.0.65532-0	2008	*
Phylogeny	[Isolation and comparative analysis of glycolipid fractions in bifidobacteria].	Novik GI, Astapovich NI, Grzegorzewicz A, Gamian A	Mikrobiologiia		2005	*
Phylogeny	Biological characterization of induced phages from Saccharopolyspora hirsuta 367 and comparison with phage JHJ-1.	Gaudreau LR, Lavoie JM, Dery CV	J Gen Microbiol	10.1099/00221287-137-10-2347	1991	*
Phenotype	Saccharopolyspora hirsuta strain 367 releases JHJ-1, a bacteriophage capable of propagation on old mycelium.	Haket J Jr, Desmarais D, Mehindate K, Dery CV	J Gen Microbiol	10.1099/00221287-136-3-573	1990	*
Phylogeny	A new broad-spectrum aminoglycoside antibiotic complex, sporaricin. II. Taxonomic studies on the sporaricin producing strain Saccharopolyspora hirsuta subsp. Kobensis nov. subsp.	Iwasaki A, Itoh H, Mori T	J Antibiot (Tokyo)	10.7164/antibiotics.32.180	1979	*

References

#11038

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43463

#19573

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20216

Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) Curators of the HKI: Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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